Helium-oxygen Mixture Research Articles

Interval inhalation application of an oxygen-helium mixture reverses the effects of cerebral arterial air embolism

Oxygen-helium mixture demonstrates marked efficacy of helium mixtures in animal models of ischemia/reperfusion, which opens the possibility of its use as an emergency measure for therapy of vascular embolism. We modelled cerebral arterial air embolism by injecting an air bubble into the internal carotid artery of awake rats. Inhalation of a heated oxygen-helium mixture immediately after modelling arterial embolism normalizes physiological abnormalities and prevents ischemic brain damage, whereas application of a heated oxygen-helium mixture 2 hours after modelling arterial embolism significantly worsens the condition of the animals.

Use of a Hyperoxic Heated Helium-Oxygen Mixture in an Experimental Model of Cerebral Arterial Air Embolism

Use of a Hyperoxic Heated Helium-Oxygen Mixture in an Experimental Model of Cerebral Arterial Air Embolism

The use of a heated helium-oxygen mixture in patients with severe COVID-19 and ARDS against the background of standard therapy and noninvasive ventilation

Introduction. One of the insufficiently studied methods of Acute Respiratory Distress Syndrome (ARDS) therapy is therapy with a heated helium-oxygen mixture. Due to physico-chemical properties, helium demonstrates high efficiency in the treatment of various diseases of the pulmonological profile.Aim. To study the effect of a heated helium-oxygen mixture on the course of a severe form of COVID-19 occurring with the development of ARDS.Materials and methods. The study involved 58 patients with severe and extremely severe coronavirus infection and ARDS, divided into the NVL + t-He/O2 group (n = 30) and the NVL+O2 control group (n = 28). In addition to standard treatment, patients of the NVL + t-He/O2 group received noninvasive ventilation (NVL) and therapy with a heated helium-oxygen mixture, according to clinical recommendations. In the main group, inhalations took place daily for 60 minutes per day for 5 days.Results. Against the background of therapy with a heated helium-oxygen mixture, a more intense increase in the level of lymphocytes was observed: 1.39 (1.09–1.66) × 109/l in the NVL + t-He/O2 group versus 1.02 (0.78–1.40) × 109/l (p = 0.02). Also, as a result of treatment, there was a significant decrease in RR, D-dimer and CRP in the study group: the level of RR (21 (20–22)/minute versus 22.5 (21–24)/minute, p = 0.003), D-dimer (0.44 (0.29–0.60) FEU ng/ml versus 0.79 (0.42–1.34) FEU ng/ml, p = 0.02) and CRP (1.1 (0.7–3.1)mg/l versus 16.6 (8.5–29.5) mg/l, p = 0.03), respectively. Similarly to the NVL + t-He/O2 group, an increase in the level of PaO2 and the oxygenation index was noted. In addition, there were significantly fewer patients with grade III and IV lung damage in the NVL + t-He/O2 group compared with the control (p = 0.05).Conclusion. The use of a heated helium-oxygen mixture in patients with severe COVID-19, occurring with the development of ARDS, against the background of standard therapy and NIV has shown its safety and effectiveness: there was a decrease in the intensity of inflammation, improved oxygenation and a reduction in the need for respiratory support.

Mathematical modeling of sound propagation through the respiratory tract in a modified gas environment

The aim of the work was to develop a mathematical model of sound propagation through the human respiratory tract when breathing certain respiratory gas mixtures. We based the model on an assembler model of branching of the respiratory tract. For calculations, we presented the human tracheobronchial tree in the form of an equivalent T-shaped cut section diagram, which includes parameters of the walls of the respiratory tract. We carried the simulation out in two main stages. At the first stage, we calculated the impedance of the flow from the small airways to the larger airways. At the second stage, we calculated the sound pressure caused by breathing. As a result, we obtained the curves of the impedance dependence on the frequency spectrum, as well as the sound power from the respiratory flow. We noted that when breathing air, the impedance value remains constant with increasing frequency. Moreover, we also noted that when exposed to a heavier gas than air, the impedance value constantly increases, and when exposed to lighter gases, after reaching a maximum, the impedance value decreases. We found that the highest sound pressure is typical for the oxygen-krypton mixture, and the lowest for the oxygen–helium mixture. We also found that in the case of modeling the laminar flow regime (with a critical Reynolds number equal to 1800), sound affects how many generations of bronchi more than in the case of modeling the transient flow regime (with a critical Reynolds number equal to 2700). Thus, we drew conclusions about the influence of the physical properties of gaseous media on the amount of sound pressure and the propagation of sound through the human respiratory tract.

Effect of a helium and oxygen mixture on physiological parameters of rats with cerebral arterial air embolism.

Introduction: Cerebral arterial air embolism (CAE) is a serious and potentially dangerous condition that can interrupt the blood supply to the brain and cause stroke. One of the promising gas mixtures for emergency treatment of air embolism is an oxygen-helium mixture. Methods: We modeled CAE in awake rats by injecting air into the common carotid artery. Immediately after CAE, animals were either untreated or underwent hyperbaria, oxygen inhalation, heated air inhalation, or helium-oxygen mixture inhalation. Body temperature, locomotor activity, respiratory and cardiovascular parameters were monitored in the animals before CAE modeling, and 3 and 24h after CAE modeling. Results: After 3 hours of CAE modeling in awake rats, depression of the nervous, cardiovascular and respiratory systems, as well as decreased body temperature were observed. 24h after CAE modeling multifocal cerebral ischemia was observed. Normobaric helium-oxygen mixture inhalation, on par with hyperbaric treatment, restored body temperature, locomotor activity, respiratory volume, respiratory rate, and blood pressure 3 hours after CAE, and prevented the formation of ischemic brain damage lesions 24h after CAE. Discussion: Thus, inhalation of a heated oxygen-helium gas mixture (O2 30% and He 70%) immediately after CAE improves the physiological condition of the animals and prevents the foci of ischemic brain damage formation.

Decompression sickness of the inner ear and relationship with a patent oval foramen: a study of 61 cases.

To discuss the link between inner ear decompression sickness and patent foramen ovale. Monocentric and retrospective study on decompression sickness of the inner ear requiring hyperbaric chamber treatment, from 2014 to 2021. Sixty-one patients of inner ear decompression sickness were included in this study. Twenty-four patients had vestibular injuries, 28 cochlear injuries and 9 cochleo-vestibular injuries. Compression chamber treatment was given, using an oxygen-helium mixture with oxygen partial pressure (PIO2) limited to 2.8 atmosphere absolute (ATA). All vestibular accidents completely recovered without clinical sequelae. For cochlear accident only 10 out of 37 patients (27%) recovered completely. A right-left shunt (patent foramen oval or intra-pulmonary shunt) was found in 31.1% of patients with inner ear decompression sickness (p > 0.05). The presence of patent foramen oval in patients with inner ear decompression was not statistically significant in our study. Understanding of the pathophysiology of decompression illness and the physiology and anatomy of the labyrinth would suggest a mechanism of supersaturation with degassing in intra-labyrinthine liquids.

Analysis of guideline recommendations for treatment of asthma exacerbations in children: a Pediatric Emergency Research Networks (PERN) study

RationaleThere is significant practice variation in acute paediatric asthma, particularly severe exacerbations. It is unknown whether this is due to differences in clinical guidelines.ObjectivesTo describe and compare the content and...

Study of the response of the microvascular bed to oxygen-helium therapy using high-frequency Doppler ultrasound in patients with COVID-19

Introduction. For the treatment of patients with hypoxemic form of respiratory failure, thermal oxygen-helium mixture (OHM) therapy is used.The aim of the work is to study the effect of OHM on the state of the microcirculatory bed in viral pneumonia caused by COVID-19 in patients with aggravated and unaggravated anamnesis.Materials and methods. There were 8 patients with an uncomplicated anamnesis, 11 with AH1–2 degree, 11 with AH1–2 degree and type II diabetes. The parameters of blood flow in the MVB were recorded with an ultrasound dopplerograph. OHM therapy (O2 – 30 %, He – 70 %, t=+95 ºС): 5 min breathing, 5 min rest – 3 cycles.Results. Before the OHM therapy, the level of «basic perfusion» was increased in group 3: significantly high Vs (p=0.02) and Vd (p=0.006), low RI (p=0.015) relative to group 1, this indicates a reduced vascular tone and venous congestion. After the OHM session in patients of group 1, the speed of Vs and Vd increased, the indices RI and PI decreased. In group 2 – Vs, Vd and Vam increased, RI decreased. The effect is long lasting. In group 3, the effect was noted on day 5, short-term. Conclusions. OHM therapy has a positive effect.

A Helium Speech Unscrambling Algorithm Based on Deep Learning

Helium speech, the language spoken by divers in the deep sea who breathe a high-pressure helium–oxygen mixture, is almost unintelligible. To accurately unscramble helium speech, a neural network based on deep learning is proposed. First, an isolated helium speech corpus and a continuous helium speech corpus in a normal atmosphere are constructed, and an algorithm to automatically generate label files is proposed. Then, a convolution neural network (CNN), connectionist temporal classification (CTC) and a transformer are combined into a speech recognition network. Finally, an optimization algorithm is proposed to improve the recognition of continuous helium speech, which combines depth-wise separable convolution (DSC), a gated linear unit (GLU) and a feedforward neural network (FNN). The experimental results show that the accuracy of the algorithm, upon combining the CNN, CTC and the transformer, is 91.38%, and the optimization algorithm improves the accuracy of continuous helium speech recognition by 9.26%.

Modelling of Nonthermal Dielectric Barrier Discharge Plasma at Atmospheric Pressure and Role of Produced Reactive Species in Surface Polymer Microbial Purification

A nonthermal atmospheric plasma reactor was used to sterilize polymer surfaces and satisfy safety constraints in a biological medium. A 1D fluid model was developed using COMSOL Multiphysics software® 5.4 with a helium-oxygen mixture at low temperature for the decontamination of bacteria on polymer surfaces. An analysis of the evolution of the homogeneous dielectric barrier discharge (DBD) was carried out through studying the dynamic behavior of the discharge parameters including the discharge current, the consumed power, the gas gap voltage, and transport charges. In addition, the electrical characteristics of a homogeneous DBD under different operating conditions were studied. The results shown that increasing voltage or frequency caused higher ionization levels and maximum increase of metastable species' density and expanded the sterilization area. On the other hand, it was possible to operate plasma discharges at a low voltage and a high density of plasma using higher values of the secondary emission coefficient or permittivity of the dielectric barrier materials. When the discharge gas pressure increased, the current discharges declined, which indicated a lower sterilization efficiency under high pressure. A short gap width and the admixture of oxygen were needed for sufficient bio-decontamination. Plasma-based pollutant degradation devices could therefore benefit from these results.

Identifying High-Performance Metal–Organic Frameworks for Low-Temperature Oxygen Recovery from Helium by Computational Screening

Metal–organic frameworks (MOFs) are an important class of porous crystalline materials for applications ranging from gas adsorption and separation to catalysis. There are thousands of potential MOFs available for separation applications. We developed a computational approach to screen MOFs for the separation of oxygen–helium mixtures at low temperatures (100–200 K), conditions that were motivated by issues associated with propulsion in space-based settings. We used detailed molecular simulations for a small number of MOFs to develop screening methods that were then used to estimate the optimum temperatures for separations using pressure swing adsorption for 2932 MOFs from the CoRE MOF database and the swing capacity and oxygen–helium selectivity at these temperatures. We used the stability of the best-performing structures in the presence of moisture as a means to provide a short list of high-performance materials. In addition to identifying specific materials for oxygen–helium separations, this approach could prove useful for selecting adsorbents for other gas separations.

Use of heated oxygen-helium mixture at the stage of rehabilitation in patients after myomectomy

Use of heated oxygen-helium mixture at the stage of rehabilitation in patients after myomectomy

Use of thermal helium-oxygen mixture for correction of respiratory failure in complex treatment of patients with cicatricial stenosis of larynx and trachea

The relevance of the work is determined by the fact that the result of treatment of patients with chronic larynx and trachea stenosis depends not only on the method of treatment, but also on the effect on postoperative tissue repair in conditions of chronic respiratory failure, which accompanies cicatricial stenosis of the larynx and trachea (CSLT). Development of new noninvasive techniques eliminating the consequences of hypoxia is a promising direction of respiratory support in the complex treatment of patients with chronic laryngeal and tracheal stenosis. The article analyzes the effectiveness of correction of respiratory disorders using a thermal helium-oxygen mixture (Heliox) in patients with chronic cicatricial stenosis of the larynx and trachea. The study included 41 patients aged 19-67 years with a disease duration of 2 months to 16 years. The necessary clinical and radiation, endoscopic and functional examination was performed on an outpatient basis. To detect chronic respiratory failure, the gas and acid-base composition of arterial blood was assessed. In the hospital, all patients underwent surgical intervention in the required volume. All patients in the pre- and postoperative period received inhalation therapy with a thermal helium-oxygen mixture according to the developed algorithm. After discharge from the hospital, a dynamic outpatient observation was carried out for 6 months. The study showed that an integrated approach to the treatment using a thermal helium-oxygen mixture (Heliox) to correct respiratory disorders and manifestations of chronic hypoxia is a promising method for pre- and postoperative management of patients with cicatricial stenosis of the larynx and trachea. Conflicts of interest. The authors have no conflicts of interest to declare. Funding. There was no funding for this study Актуальность работы обусловлена тем, что результат лечения больных хроническим стенозом гортани и трахеи зависит не только от метода лечения, но и от воздействия на послеоперационную репарацию тканей в условиях хронической дыхательной недостаточности, которая сопутствует рубцовому стенозу гортани и трахеи (РСГТ). Разработка новых неинвазивных методик, устраняющих последствия гипоксии, является перспективным направлением респираторной поддержки в комплексном лечении пациентов с хроническим стенозом гортани и трахеи. Цель исследования: анализ эффективности коррекции дыхательных нарушений при помощи использо- вания термической гелий-кислородной смеси (Гелиокс) у пациентов с хроническим РСГТ. Материал и методы. В исследование включен 41 пациент с возрастным интервалом 19–67 лет и дли- тельностью заболевания от 2 месяцев до 16 лет. Амбулаторно было проведено необходимое клинико- лучевое, эндоскопическое и функциональное обследование. Для выявления хронической дыхательной недостаточности исследовали газовый и кислотно-щелочной составы артериальной крови. В стационаре всем пациентам проводилось хирургическое вмешательство в необходимом объеме. Все пациенты в до- и послеоперационном периодах получали ингаляционную терапию термической гелий-кислородной смесью по разработанному алгоритму. После выписки из стационара проводилось динамическое амбулаторное наблюдение в течение 6 месяцев. Результаты. Исследование показало, что комплексный подход к лечению больных с РСГТ с применением термической гелий-кислородной смеси (Гелиокс) для коррекции дыхательных нарушений и проявлений хро- нической гипоксии, является перспективным методом до- и послеоперационного ведения пациентов с РСГТ. Конфликт интересов. Авторы заявляют об отсутствии конфликта интересов. Финансирование. Работа выполнена без спонсорской поддержки.

Sterilization mechanism of helium/helium–oxygen atmospheric-pressure pulsed dielectric barrier discharge on membrane surface

Pulsed dielectric barrier discharge (PDBD) exhibits several applications in different fields; however, the interaction of its components with substances remains a key issue. In this study, we employed experimental and numerical modeling to investigate the interactions between different PDBD components and substances in pure helium and a helium–oxygen mixture. A membrane comprising a Staphylococcus aureus strain was utilized as the treatment object to demonstrate the trace actions of the evolutions and distributions of certain components on the surface of the substance. The results revealed that the shapes and sizes of the discharging area and inhibition zone differed between groups. Under a pure helium condition, a discharge layer existed along the membrane surface, lying beside the main discharging channel within the electrode area. Further, an annulus inhibition zone was formed at the outer edge of the electrode in the pure helium group at 30 s and 1 min, and this zone extended to a solid circle at 2 min with a radius that was ∼50% larger than that of the electrode radius. Nevertheless, the discharging channel and inhibition zone in the helium–oxygen mixture were constrained inside the electrode area without forming any annulus. A 2D symmetrical model was developed with COMSOL to simulate the spatiotemporal distributions of different particles over the membrane surface, and the result demonstrated that the main components, which formed the annulus inhibition zone under the pure helium condition, contributed to the high concentration of the He+ annulus that was formed at the outer edge of the electrode. Moreover, O+ and were the main components that killed the bacteria under the helium–oxygen mixture conditions. These results reveal that the homogenization treatment on a material surface via PDBD is closely related to the treatment time and working gas.

Evaluation of the Efficiency of Oxygen-Helium Therapy for Patients with Covid-19-Associated Pneumonia.

This article summarizes the experience of managing patients with COVID-19-associated pneumonia treated in one of the intensive care units of a multidisciplinary hospital with and without a heated oxygen-helium mixture. It has been shown that the use of a heated oxygen-helium mixture in the complex therapy of pneumonia caused by SARS-CoV-2 is effective. A comparative analysis of the clinical manifestations of the disease and the results of laboratory tests in the main and control groups of patients confirm with a high degree of reliability the improvement of blood oxygenation, the normalization of the acid-base balance and the positive dynamics of the main parameters, which ultimately increases the efficacy and reduces the time of treatment.

Application of pharmaco-physiotherapeutic technologies to improve local light sensitivity in inflammatory optic neuropathy

In different years, various groups of drugs, including in combination with physiotherapeutic methods (laser, electrical and magnetic stimulation, electrophoresis with various drugs), were used to treat patients with optic neuropathies. However, no significant results have been achieved so far, and the issue of developing and applying complex pharmaco-physiotherapeutic programs that include two or more factors to potentiate the effects and increase the treatment efficacy remains relevant. Objective: to study in a comparative aspect the effect of transcranial magnetic-electrical stimulation and endonasal electrophoresis with the neuroprotective drug «Semax» 0.1 % and an extended complex, including oxybarotherapy in alternation with inhalation of the helium-oxygen mixture «Heliox 21» and acupuncture, on local light sensitivity in patients with inflammatory optic neuropathy. Material and research methods. The study included 71 patients (142 eyes) with a verified diagnosis of inflammatory optic neuropathy. The patients were divided into 3 groups comparable in terms of clinical, functional, gender, and age characteristics: the main group included 23 people (46 eyes) who had a course of extended neuro-ophthalmic stimulation, consisting of transcranial magnetic-electrical stimulation, endonasal electrophoresis with the neuroprotective drug «Semax» 0.1 %, oxybarotherapy alternating with inhalation of helium-oxygen mixture «Heliox 21», and acupuncture; control group 1 included 24 patients (48 eyes) who underwent a course of transcranial magnetic-electrical stimulation and endonasal electrophoresis with the Semax neuroprotective drug; control group 2 included 24 patients (48 eyes) who were prescribed basic therapy, including a vitamin complex Berocca for 3 months, then a break of 1 month, and repeated courses with an alternation of 3:1, which served as a background in the main group and control group 1. Results. Under the influence of an extended course of complex physiotherapy, more significant and lasting results were obtained in the form of an increase in indicators of the threshold of light sensitivity both in the best and in the worst eye, which persisted throughout the entire follow-up period (up to 48 weeks). By comparison, when using a course of physiotherapy, including transcranial magnetic-electrical stimulation and endonasal electrophoresis with a neuroprotective drug «Semax» (control 1), the efficiency was significantly lower, and significant positive dynamics was noted only in the worst eye, it was less pronounced and persisted for up to 24 weeks. Conclusion. Using the course of extended neuro-ophthalmic stimulation in patients with inflammatory optic neuropathy, including transcranial magnetic-electrical stimulation, endonasal electrophoresis with the neuroprotective drug «Semax» 0.1 %, oxybarotherapy in alternation with inhalation of helium-oxygen mixture «Heliox 21», and acupuncture, contributes to an increase in the threshold light sensitivity in both the best and worst eye and its long-term preservation during the entire follow-up period (up to 48 weeks).

The Effects of H2S and Recombinant Human Hsp70 on Inflammation Induced by SARS and Other Agents In Vitro and In Vivo.

The ongoing epidemic caused by SARS-CoV-2 infection led to the search for fundamentally new ways and means to combat inflammation and other pathologies caused by this virus. Using a cellular model of lipopolysaccharide (LPS)-induced sepsis (human promonocytes), we showed that both a hydrogen sulfide donor (sodium thiosulfate, STS) and a recombinant Heat shock protein 70 (rHsp70) effectively block all major inflammatory mediators when administrated before and after LPS challenge. The protective anti-inflammatory effect of rHsp70 and H2S was also confirmed in vivo using various animal models of pneumonia. Specifically, it was found that rHsp70 injections prevented the development of the acute respiratory distress syndrome in highly pathogenic pneumonia in mice, increased animal survival, and reduced the number of Programmed death-1 (PD-1)-positive T-lymphocytes in peripheral blood. Based on our model experiments we developed a combined two-phase therapeutic approach for the treatment of COVID-19 patients. This procedure includes the inhalation of hot helium–oxygen mixtures for induction of endogenous Hsp70 in the first phase and STS inhalation in the second phase. The use of this approach has yielded positive results in COVID-19 patients, reducing the area of lung lesions, restoring parameters of innate immunity and T-cell immune response against coronavirus infection, and preventing the development of pulmonary fibrosis and immune exhaustion syndrome.

Intraoperative Management of a Severely Kinked Endotracheal Tube and Difficult Airway.

This figure is a lateral cervical radiograph of a nasally intubated 16-yr-old patient with Crouzon syndrome obtained after completion of cranial fossa surgery for Chiari malformation and returning to supine from a prone position. The standard endotracheal tube was severely kinked in the nasopharynx, as shown in the image, and the location of the kinking is indicated with a red arrow. This was likely caused by the warming of the endotracheal tube and the patient’s unique pharyngeal anatomy. The tracheal intubation of the patient was difficult, requiring 11 attempts due to midface hypoplasia, obesity, macroglossia, and cervical spine instability. After the start of surgery, elevated airway pressures and the inability to pass a suction catheter suggested kinking of the endotracheal tube. Due to extremely difficult intubation, endotracheal tube replacement was not attempted. Because the severely kinked endotracheal tube limited inspiratory and expiratory airflow, adequate tidal volume was achieved by increasing peak inspiratory pressure.1 Intermittent measurement of plateau pressure with an expiratory hold yielded additional information about the pressure at the alveoli. Because expiration was passive, increasing the expiratory time facilitated exhalation and reduced the risk of breath stacking.2 A helium–oxygen mixture can also be used to improve ventilation by reducing gas density, increasing the likelihood of maintaining laminar flow across the point of obstruction, and improving bulk flow.3 If the patient can tolerate permissive hypercapnia, a reduction in minute ventilation is also an option to minimize turbulent flow. The use of a wire-reinforced tube is often recommended to prevent endotracheal tube kinking in such a patient.The authors declare no competing interests.

Вreathing in helium-oxygen gas mixtures as a means of emergency restoration of the working capacity of specialists of hazardous occupations

OBJECTIVES: To evaluate the effectiveness of using helium-oxygen breathing mixtures for emergency restoration of the working capacity of specialists of hazardous occupations.MATERIALS AND METHODS: 18 male military personnel (age 25–40 years), divided into the main group and the comparison group (9 people each). All servicemen showed signs of decreased efficiency. In the main group, a procedure of 40-minute breathing with a heated gas mixture with an Oxygen content of 25% vol., Helium content of 75% vol. was prescribed. The comparison group underwent standard 40-minute Oxygen therapy.RESULTS: Using of helium-oxygen gas media demostrated much more effective means of emergency restoration of the work capacity of specialists than oxygen therapy. This was evidenced by the better dynamics of physical performance indicators and the level of physiological reserves in the main group.CONCLUSIONS & DISCUSSION: Helium-oxygen therapy can be considered as an effective and safe method of restoring working capacity, applicable in the «field» conditions.

The use of heated oxygen-helium mixture in patients with arterial hypertension

BACKGROUND: The spread of arterial hypertension is associated with the presence of such risk factors as smoking, excess body weight, violation of lipid metabolism, the current psycho-emotional load.
 AIMS: to assess the effectiveness of the use of heated oxygen-helium mixture in the rehabilitation of patients with arterial hypertension at the outpatient polyclinic stage.
 MATERIAL AND METHODS: The supervision was 67 people aged from 40 to 56 years (48.254.12 years old) with a verified diagnosis: arterial hypertension I and II art., Low and medium risk, the duration of the disease was 7 years to 15 years (8.982.2 years). Patients were divided into two groups: I Group (n=35) the main, patients underwent a course of rehabilitation with the inclusion of oxygen-helium mixture; group II (n=32) comparisons, patients underwent a course of rehabilitation without the use of mixture. Breathing was carried out in cyclical and fractionated mode: the breathing mixture is 5 minutes then breathing with atmospheric air 5 minutes (one cycle), 3 such cycles for the procedure. The course of treatment consisted of 1 procedure per day within 10 days from the first day of the study.
 To assess the clinical efficiency of reducing treatment, the study was carried out before the start of regenerative treatment and at the end of the course. The effectiveness of this method was assessed by the following indicators: assessment of vegetative status using cardiac rhythm variability indicators; evaluation of blood flow and elastic-elastic properties in the distal arteries of the lower extremities; evaluation of the adaptation of the cardiovascular system to physical exertion with the help of the Harvard Step test, a 6-minute walking test; the level of cortisol in saliva was determined using the immunosyluminescence method; the level of depression and anxiety.
 RESULTS: In the main group of patients, there was a positive dynamics in the form of normalization of vegetative status, an increase in the activity of the parasympathetic department of the autonomic nervous system, a decrease in the tone of the sympathetic nervous system, the improvement of elastic properties at the level of the distal arteries of the leg (rear tibial artery, the artery of the foot). After the course of respiration, an increase in physical exhaust tolerance was revealed from the patients examined with arterial hypertension, which passed the respiratory rate of heated oxygen-helium mixture and increase the speed of adaptation processes of recovery of the cardiovascular system after exercise. This analysis of the dynamics of the level of cortisol in saliva demonstrated a tendency to reduce the level in patients of the main group. The results of the study indicate the improvement of the psycho-emotional state of patients with arterial hypertension.
 CONCLUSION: The use of heated oxygen-helium mixture has a positive effect on the functional state of patients with arterial hypertension.