Helium-neon Laser Irradiation Research Articles

Effect of three-volt moxibustion with helium-neon laser irradiation on quality of care in patients with lumbar radiculopathy spondylosis.

Lumbar radiculopathy spondylosis is a relatively common orthopedic disease with a high incidence rate. It most commonly occurs in the lumbar 4-5 and lumbar 5-sacral 1 vertebrae, which account for approximately 95% of cases. It mostly occurs in people aged 30-50 years old and greatly affects their quality of life. To determine the effect of triple-voltage acupuncture combined with helium-neon laser irradiation on the quality of care and improvement of symptoms in patients with lumbar radiculopathy spondylolisthesis. In this study, we selected 120 patients with lumbar radiculopathy spondylosis who were treated at our hospital between June 2019 to June 2020. The patients were divided into control and observation groups according to the random number table method, with 60 patients in each group. Patients in the observation group were treated with three-volt moxibustion combined with helium-neon laser irradiation, and those in the control group were treated with lumbar traction. After 1 month of treatment, the lumbar pain scores, lumbar spine motor functions, clinical treatment effects, and nursing satisfaction of the two groups were compared. The results showed that acupuncture combined with laser irradiation significantly improved the patients' clinical symptoms, i.e., reduced their low back pain, significantly lower numerical rating scale pain scores in the observation group than in the control group, and better lumbar spine motility than in the control group, compared to lumbar traction. In addition, they were cared for. The treatment effectiveness rate of the observation group was 95.5%, which was significantly higher than that of the control group (81.67%). Satisfaction with care was higher than 90 points in both groups, but the difference was not statistically significant. Our study provides a clinical rationale for the future treatment of patients with lumbar spine disease. However, further extensive research is needed for validation.

Influence of laser photoactivated graphitic carbon nitride nanosheets and nickel nanoparticles on purple non-sulfur bacteria for biohydrogen production from biomass

The objective of this study is to increase biohydrogen production from biomass using laser photoactivated nanomaterials. Light saturation of photo-fermentation is a vital factor that influences fermentation effectiveness and hydrogen yield. In this study, it is hypothesized that the bio-stimulation of hydrogen-producing purple non-sulfur (PNS) bacteria using laser photoactivated nanomaterials can enhance the endurance ability of such bacteria to unsteady light irradiation, where this leads to overcome the challenge of light saturation. Furthermore, the addition of nanomaterials leads to bio-stimulate the bacterial cells and enhance their activity and growth rate and, therefore, increase biohydrogen production from biomass. A biohydrogen production system and a model of photobioreactor were manufactured and installed. Food wastes were collected from kitchen leftovers of different fast-food suppliers and were used in this study as feedstocks for biohydrogen production. The production process was conducted as following: exposing 16.5 mg/l of graphitic carbon nitride nanosheets on the one hand and 50 mg/l of nickel nanoparticles on the other hand to a helium-neon green laser radiation source with a wavelength of 543 nm for 1 h, then adding them to the bacterial inoculum and then mixing them with biomass and water by a ratio of 0.5:1:2 which were then kept in the photobioreactor exposed to white light emitting diodes (LEDs) with a luminous flux of 3600 lumen and at 30 °C for 26 days with mixing for 5 min every 30 min to produce biohydrogen. By this method, it is possible to improve the bioenvironmental conditions and the bio-responses of bacteria which results in increasing the biohydrogen yield by 287% over the conventional method.

LICHEN SCLEROSUS OF THE VULVA IN PATIENTS WITH LIMITED SCLERODERMA

Modern literature information on the etiopathogenetic prevalence of lichen sclerosus has been presented by the authors; clinical manifestations and classification of the disease have been outlined; diagnosis and treatment issues have been discussed in the article. 3 clinical cases of genital lesions in the form of lichen sclerosus in patients with limited scleroderma have been described. In our opinion, lichen sclerosus is one of the forms of limited scleroderma.
 Purpose. To investigate the conditions for the diagnosis and treatment of genital lesions in the form of lichen sclerosus in patients with limited scleroderma.
 Methods. The first line of therapy for lichen sclerosus is the use of super-powerful topical glucocorticoids. Clobetasol propionate 0.05% is to be used for 3 months (1 month daily, then every other day for a month, then 2 times a week for a month). After 3 months of treatment, if a positive effect is declared, maintenance therapy is recommended once a week. Topical calcineurin inhibitors are recommended as a second-line therapy. Effective and safe excipients are emollients that moisturize the skin and mucous membranes, prevent transdermal fluid loss, and reduce subclinical inflammation. The possibility of using triamcinolone (1-2 mg) onto pathologically altered tissue in women with lichen sclerosus where there are hyperkeratotic sites resistant to GCS can be considered after exclusion of intraepithelial neoplasia or malignant transformation. The effectiveness of physiotherapeutic methods, such as low-intensity helium-neon laser radiation in the red or infrared range, treatment with sunlight and radiation generated by medical equipment, photodynamic therapy has been described.
 Results. One of the important measures is the elimination of irritating factors, as well as careful care of the vulva and the treatment of secondary infection. It consists in adherence to a diet (exclusion of spicy, salty, sweet foods, caffeine-containing products, alcohol) and intimate hygiene rules (limitation / exclusion of soap-containing products, deodorants, synthetic underwear, pads, tampons). The underwear should not be very tight. In addition, patients should exclude mechanical irritation of the affected area, including shaving. With extreme manifestation of itching, it is recommended to prescribe desensitizing therapy and sedatives.
 Conclusions. Clinical cases of practical interest in connection with the clinical manifestations of limited scleroderma on the skin and genitals have been considered. Frequent lesions of the genital organs in the form of a typical clinic of lichen sclerosus in women suffering from limited scleroderma, the similarity of their clinic, histological picture and pathogenesis have been noted. All this gives a reason to consider lichen sclerosus as a manifestation of limited scleroderma.

Laser irradiation pretreatment improves endometrial preparation of frozen-thawed embryo transfer in recurrent implantation failure patients

Recurrent implantation failure (RIF) remains a clinical dilemma. Helium-Neon (He-Ne) laser irradiation has recently become more popular under certain clinical conditions. Given the unique therapeutic effects, we were interested in determining whether pretreatment with He-Ne laser irradiation prior to frozen-thawed embryo transfer (FET) would improve the microcirculation and cause the release of growth factors and cytokines, thus improving endometrial receptivity and the clinical pregnancy rates. Patients chose for themselves whether to proceed with (n = 29) or without (n = 31) pretreatment with He-Ne laser irradiation prior to FET. The clinical pregnancy rate (37.9%) and implantation rate (20.3%) were higher in the laser-treatment group than in the control group (35.5% and 15.9%, respectively, p = .844 and .518, respectively). The live birth rate was higher in the laser-treatment group (27.6% vs. 25.8%, respectively, p = .876) and the miscarriage rate was lower in the laser-treatment group (18.2% and 27.3%, respectively, p = .611). No side effects or complications from laser irradiation were encountered in patients who received the laser treatment. We concluded that pretreatment with He-Ne laser prior to FET may be an alternative choice for RIF-affected women; however, additional well-designed prospective studies are necessary to determine the precise clinical value of this treatment.

Recent advances in cryopreservation of semen and artificial insemination in sheep: A review

Cryopreservation of semen facilitates widespread dissemination of superior germplasm and thus allowing the full exploitation of artificial insemination (AI) benefits. Recent focus is on preserving fertilizing capacity of frozen spermatozoa by improving the composition of extenders and changing cooling/freezing protocols, use of cryoprotectants, antioxidants, including enzymes, vitamins, amino acids and proteins, plant extracts and other compounds. In addition, research is also intensified on role of seminal plasma and its proteins as well as cholesterol-loaded cyclodextrin in semen freezing. Recently it has also been shown that helium-neon laser irradiation of cryopreserved ram sperm increased the post-thaw sperm motility, viability, cytochrome c oxidase activity and ATP contents with no significant changes in DNA and acrosome integrity. Post-thaw sperm motility of cryopreserved semen ranged from 33.3 to 72.2%. Fertility depends on the insemination technique and ranged from 45 to 80% for laparoscopic and 4 to 42% for transcervical insemination. Further, the complex anatomy of ewe's cervix is also a hurdle affecting intrauterine deposition of semen and consequently the fertility of frozen-thawed semen. Researchers are attempting cervical dilation with the use of hormones and chemicals to facilitate catheter penetration in the cervical canal. This review gives a detailed semen processing techniques and their effects on semen quality and AI techniques used in sheep and their success rates.

Effect of Laser Irradiation on Cell Cycle and Mitosis.

Introduction: In this research, low-level helium-neon (He-Ne) laser irradiation effects on monkey kidney cells (Vero cell line) mitosis were studied. Methods: The experiment was carried out on a monkey kidney cell line "Vero (CCL-81)". This is a lineage of cells used in cell cultures and can be used for efficacy and media testing. The monolayer cells were formed on coating glass in a spectral cuvette (20×20×30 mm). The samples divided into two groups. The first groups as irradiated monolayer cells were exposed by a He-Ne laser (PolyaronNPO, L'vov, Ukraine) with λ=632.8 nm, max power density (P) = 10 mW/cm2 , generating linearly polarized and the second groups as the control monolayer cells were located in a cuvette protected by a lightproof screen from the first cuvette and also from the laser exposure. Then, changing functional activity of the monolayer cells, due to the radiation influence on some physical factors were measured. Results: The results showed that low-intensity laser irradiation in the range of visible red could make meaningful changes in the cell division process (the mitosis activity). These changes depend on the power density, exposure time, the presence of a magnetic field, and the duration of time after exposure termination. The stimulatory effects on the cell division within the power density of 1-6 mW/(cm2 ) and exposure time in the range of 1-10 minutes was studied. It is demonstrated that the increase in these parameters (power density and exposure time) leads to destructing the cell division process. Conclusion: The results are useful to identify the molecular mechanisms caused by low-intensity laser effects on the biological activities of the cells. Thus, this study helps to optimize medical laser technology as well as achieving information on the therapeutic effects of low-intensity lasers.

The influence of Helium-Neon laser irradiation on wound healing

The influence of Helium-Neon laser irradiation on wound healing

Features of dogs treatment at fractures of peripheral skeleton

The article deals with the results of research on the distribution of fractures of the bones of the peripheral skeleton in dogs depending on the breed, age, and also the features of treatment of animals in the fracture of the limb bones by the use of osteosynthesis in combination with composite materials, which contributes to the improvement of tactics and methods of injured dogs treating. It has been established that the largest percentage of fractures of the limb bones in dogs falls on young animals, but in general the age range is considerably wider and ranges from 4–5 months to 8 years. Treatment of dogs for fractures of limb bones is carried out by application of various methods of osteosynthesis. However, to prevent complications in the form of infection, the formation of adhesions, the slowing of osteogenesis, etc., use physical methods of stimulation of reparative osteogenesis (variable high-frequency electromagnetic field, low-intensity helium-neon laser irradiation, low-frequency ultrasound). Considering that osteosynthesis is an additional trauma that causes a higher reaction rate of the acute phase, a number of searches have found the feasibility of using pharmacological correction agents, in particular, «Collapan-L», «Biomin-GT», fibrin gel etc. Today optimization of reparative regeneration, in particular of the bones of the peripheral skeleton in dogs, is carried out at the expense of vitamin-mineral, anti-inflammatory, antibacterial, antioxidant, immunomodulatory, hormonal and other groups of pharmacological preparations. However, in veterinary orthopedics the use of osteotropic materials to replace defects of bone tissue and stimulate reparative osteogenesis are devoted only a few research papers. Therefore, the development and implementation of new tactics and methods of treating of injured dogs by the combined use of osteosynthesis and new effective pharmacological medicines remains relevant.

Mutations rate in different lines of drosophila melanogaster after the treatment with caffeine and He-Ne laser radiation

The frequency of dominant lethal mutations (DLM) after combined effect of caffeine (1,3,7-trimethylxan- thine) and helium-neon laser radiation in Drosophila has been studied. Caffeine is a natural substance which is presented in many drinks, widely applied in medicine and reveals moderately toxic action and some mutagen- icity/genotoxicity effects; red laser radiation is applied in medicine and can disrupt the processes of DNA and RNA biosynthesis. The aim of this work was to study the characteristics of the mutation process in Drosophila melanogaster under the influence of caffeine and red laser radiation, depending on the genotype. We used wild type Drosophila melanogaster stocks Canton-S (C-S) and Oregon (Or), carrying an ebony mutation, respectively: ebonyC-S and ebonyOr; and mutant stock ebony. Control flies were grown in standard conditions, and in experiments with caffeine it was added in culture medium in concentration 0.5 mg/ml. Vir- gin flies were exposed to a helium-neon laser light (wavelength of 632,8 nm) with a surface power density of 0.03 mW/cm2, the exposure time was 5 minutes. The influence of caffeine causes an increase in the frequency of early DLM on 40 % in mutant line ebony, and on 70.5 % in the line ebonyC-S. Irradiation by light of helium-neon laser either reduces the incidence DLM, or has no effect in all lines used in this work. The simultaneous effect of two external factors induces diverse answers in different genotypes: it increases early DLM in line C-S and early and late DLM in line ebony; but has no effect in lines ebonyOr and ebonyC-S. The two-way analysis ANOVA F-test showed that the DLM frequency dependence impact of external factors is characterized by F= 3.87; of genotype F= 5.14; of the combined effects of genotype and external factors F = 5.69. The power of influence of external factors — 8.1 %; of genotype 6.9 %; and of the combined effects of the genotype and two external factors studied — 31.4 %. Conclusions. Irradiation with laser light had no mutagenic effect on Drosophila, and even revealed anti- mutagenic effect. Caffeine induced the DLM increase, but the combined caffeine and laser light impact increased DLM rate in line ebony and not influenced it in lines: ebonyOr and ebonyC-S.

Эндоокулярное биостимулирующее воздействие низкоинтенсивного излучения гелий-неонового лазера 0,63 мкм на клетки заднего эпителия роговицы в процессе экстракции катаракты

Эндоокулярное биостимулирующее воздействие низкоинтенсивного излучения гелий-неонового лазера 0,63 мкм на клетки заднего эпителия роговицы в процессе экстракции катаракты

Formation of long-lived reactive species of blood serum proteins induced by low-intensity irradiation of helium-neon laser and their involvement in the generation of reactive oxygen species

It was demonstrated that low-intensity radiation of helium-neon (He-Ne) laser at 632.8nm, which leads to the transition of oxygen to a singlet state, causes the formation of reactive oxygen species (ROS) – hydrogen peroxide, hydroxyl and superoxide (hydroperoxide) radicals – in aqueous solutions. The oxygen effect – dependence of hydrogen peroxide formation on the concentration of molecular oxygen – was shown, and the participation of singlet oxygen, hydroxyl radicals and superoxide (hydroperoxide) radicals in this process was testified. Laser radiation-induced ROS in solutions of blood serum proteins, bovine serum albumin and bovine gamma-globulin, cause the formation of long-lived reactive protein species (LRPS) with a half-life of about 4h. The generation of LRPS caused by laser irradiation results in prolonged several-hour generation of ROS – hydrogen peroxide, hydroxyl and superoxide radicals. As affected by LRPS, coupled radical reactions lead to conversion of dissolved molecular oxygen to hydrogen peroxide. Irradiation with light sources away from the oxygen absorption band is not attended by formation of ROS and LRPS. A consideration is provided for the possible molecular mechanisms of ROS formation under the influence of He-Ne laser irradiation, the role of proteins in their generation and the biological significance of these processes.

In vitro culture responses, callus growth and organogenetic potential of brinjal (Solanum melongena L.) to He-Ne laser irradiation

The present investigation was designed to analyze the influence of Helium-Neon (He-Ne 632.8nm) laser irradiation on defense enzymes, proline content and in vitro responses of callus induction, shoot initiation and on plantlet regeneration potential of brinjal. The seeds of Mattu Gulla (Solanum melongena L.) were irradiated with 20, 25 and 30J/cm2 of He-Ne laser followed by surface sterilization and sprouted on Murashige and Skoog medium without plant growth regulators. The activity of defense enzymes, proline content and the organogenetic potential of hypocotyl, leaf and shoot tip explants were determined from thirty day old seedlings. During seed germination, most of the seedlings showed normal two cotyledons whereas small number of seedlings showed tricotyledonous at 20J/cm2 treatment and no other morphological abnormalities were observed during further growth and development. There was no substantial variation was noted in both β-1,3-glucanase and chitinase activity as well as proline content which proves the He-Ne laser irradiation does not causes any stresses for the plant. The in vitro culture of hypocotyl, leaf and shoot tip explants from laser irradiated seedlings showed differential responses as compared to un-irradiated control. The laser induced enhancement of callus induction, growth rate of callus tissues and shoot tip, percentage of responses of shoot and root initiation, days to shoot and root initiation, shoots formed per callus, number of roots per shoots, length of roots and nuclear DNA content of in vitro raised plants were evaluated. Among the tested laser doses (20, 25 and 30J/cm2), 25J/cm2 showed significant biostimulatory effect over un-irradiated control seedlings. The present observations reveal and endorsed our earlier reports with substantial enhancement of in vitro and ex vitro by He-Ne laser irradiation.

Intravascular low-level helium-neon laser irradiation on blood for acute ischemic stroke

Reason for withdrawal from publication This protocol has been published since 2010. It has been withdrawn from publication because the authors have been unable to complete the full review.

Responses of He-Ne laser irradiation on agronomical characters and chlorogenic acid content of brinjal (Solanum melongena L.) var. Mattu Gulla

Exposure to laser irradiation on seeds brings about the changes in agronomical characteristics of the plants. Solanum melongena L. var. Mattu Gulla, a variety of brinjal is of high economic value due to its unique colour and flavour. The aim of the study was to understand the influence of Helium-Neon (He-Ne) laser irradiation on agronomical characters of Solanum melongena L. var. Mattu Gulla in the field conditions. Various growth characteristics including seed germination percentage, survival rate, plant height, number of branches, and flowers and fruits were estimated during different developmental stages of the brinjal. In addition, the chlorogenic acid content of fruits obtained from the laser irradiated seeds were quantified using reversed-phase high-performance liquid chromatography (RP- HPLC). The plants from the seeds irradiated with different doses (20, 25 and 30J/cm2) of He-Ne laser showed significant enhancement on the growth characteristics when compared to the non-irradiated control groups. He-Ne laser irradiation also improved the yield characteristics of the plants significantly in in vivo conditions in comparison with control group. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed using methanolic extract of matured fruit of Mattu Gulla on HepG2 and fibroblast cell lines. The IC50 values of fruit extract from laser irradiated groups were found to be similar to non-irradiated control groups. Chlorogenic acid content was found to be higher in 20J/cm2 and lower in 30J/cm2 treated fruit tissue. The current study thus elucidates the role of He-Ne laser as a biostimulator on brinjal var. Mattu Gulla not only in the in vitro conditions but also in the in vivo field conditions.

Combined Effect of Low-Intensity Helium-Neon Laser and X-Ray Radiation on in Vivo Cellular Response of the Whole Blood and Lymphoid Organs in Mice.

We studied the effect of exposure to helium-neon laser (dose range 0.16-50 mJ/cm2) on activation of natural protection reserve in mice using the adaptive response test. DNA comets method revealed a protective response manifested in DNA damage level in whole blood leukocytes of mice and in lymphoid organs by the thymus and spleen weight index; preexposure to laser did not induce the adaptive response. ROS level in the whole blood was assessed by the level of zymosan-induced luminol chemiluminescence. In mice subjected to adaptive laser irradiation in doses of 0.16-5 mJ/cm2 followed by X-ray irradiation in a dose of 1.5 Gy, the activation index calculated as the ratio of induced to spontaneous area of luminescence was by 1.4 times lower than that in non-irradiated animals, which attested to reduced ROSgeneration reserve capacity of neutrophils.

Helium-neon laser irradiation of cryopreserved ram sperm enhances cytochrome c oxidase activity and ATP levels improving semen quality

This study examines whether and how helium-neon laser irradiation (at fluences of 3.96–9 J/cm2) of cryopreserved ram sperm helps improve semen quality. Pools (n = 7) of cryopreserved ram sperm were divided into four aliquots and subjected to the treatments: no irradiation (control) or irradiation with three different energy doses. After treatment, the thawed sperm samples were compared in terms of viability, mass and progressive sperm motility, osmotic resistance, as well as DNA and acrosome integrity. In response to irradiation at 6.12 J/cm2, mass sperm motility, progressive motility and viability increased (P < 0.05), with no significant changes observed in the other investigated properties. In parallel, an increase (P < 0.05) in ATP content was detected in the 6.12 J/cm2-irradiated semen samples. Because mitochondria are the main cell photoreceptors with a major role played by cytochrome c oxidase (COX), the COX reaction was monitored using cytochrome c as a substrate in both control and irradiated samples. Laser treatment resulted in a general increase in COX affinity for its substrate as well as an increase in COX activity (Vmax values), the highest activity obtained for sperm samples irradiated at 6.12 J/cm2 (P < 0.05). Interestingly, in these irradiated sperm samples, COX activity and ATP contents were positively correlated, and, more importantly, they also showed positive correlation with motility, suggesting that the improved sperm quality observed was related to mitochondria-laser light interactions.

The effect of low-level helium-neon laser on oral wound healing.

Background:The effectiveness of low power lasers on incisional wound healing, because of conflicting results of previous studies, is uncertain. Therefore, the aim of this study was to evaluate the effects of low-level helium-neon (He-Ne) laser irradiation on wound healing in rat's oral mucosa.Materials and Methods:Sixty-four standardized incisions were carried out on the buccal mucosa of 32 male Wistar divided into four groups of eight animals each. Each rat received two incisions on the opposite sides of the buccal mucosa by a steel scalpel. On the right side (test side), a He-Ne laser (632 nm) was employed on the incision for 40 s. Laser radiation was used just in 1st day, 1st and 2nd day, 1st and 3rd day, and continuous 3 days in groups of A, B, C, and D of rats, respectively. The left side (control side) did not receive any laser. Histological processing and hematoxylin and eosin staining were done on tissue samples after 5 days. Wilcoxon and Kruskal-Wallis tests were used for statistical analysis.Results:Histological analysis showed that the tissue healing after continuous 3 days on the laser irradiated side was better than the control side, but there was no difference between the two sides in each groups (P > 0.05).Conclusion:This study showed that He-Ne laser had no beneficial effects on incisional oral wound healing particularly in 5 days after laser therapy. Future research in the field of laser effects on oral wound healing in human is recommended.

Changes in the germination process and growth of pea in effect of laser seed irradiation

Abstract The aim of this study was to determine the effect of pre-sowing helium-neon (He-Ne) laser irradiation of pea seeds on changes in seed biochemical processes, germination rate, seedling emergence, growth rate, and yield. The first experimental factor was exposure to laser radiation: D0 - no irradiation, D3 - three exposures, D5 - five exposures, and the harvest dates were the second factor. Pre-sowing treatment of pea seeds with He-Ne laser light increased the concentrations of amylolytic enzymes and the content of indole-3-acetic acid (IAA) in pea seeds and seedlings. The exposure of seeds to He-Ne laser light improved the germination rate and uniformity and modified growth stages, which caused acceleration of flowering and ripening of pea plants. Laser light stimulation improved the morphological characteristics of plants by increasing plant height and leaf surface area. Irradiation improved the yield of vegetative and reproductive organs of pea, although the effects varied at the different growth stages. The increase in the seed yield resulted from a higher number of pods and seeds per plant, whereas no significant changes were observed in the number of seeds per pod. Both radiation doses exerted similarly stimulating effects on pea growth, development, and yield.

Біометричний аналіз прооксидантно-антиоксидантної рівноваги за дії гелій-неонового лазерного випромінювання та озонованого фізіологічного розчину в крові хворих на туберкульоз легень на тлі хіміотерапії

Біометричний аналіз прооксидантно-антиоксидантної рівноваги за дії гелій-неонового лазерного випромінювання та озонованого фізіологічного розчину в крові хворих на туберкульоз легень на тлі хіміотерапії

Helium-neon laser therapy in the treatment of hydroxyapatite orbital implant exposure: A superior option.

The aim of the present study was to evaluate the efficacy of helium-neon laser therapy in the treatment of hydroxyapatite orbital implant exposure and compare the results with those of a combined drugs and surgery regimen. A total of 70 patients with hydroxyapatite orbital implant exposure in 70 eyes were randomly divided into two groups: Helium-neon laser therapy (group A) and drugs plus surgery (group B). Each group contained 35 patients. The healing rates and times of the conjunctival wound were recorded and compared following helium-neon laser treatment or the drugs plus surgery regimen. Changes in the hydroxyapatite orbital implant prior to and following helium-neon laser irradiation were analyzed. A similar animal study was conducted using 24 New Zealand white rabbits, which received orbital implants and were then received drug treatment or helium-neon therapy. In the human experiment, the rates for conjunctival wound healing were 97.14% in group A and 74.29% in group B, with a significant difference between the groups (χ2=5.71, P<0.05). Patients with mild exposure were healed after 7.22±2.11 days of helium-neon laser therapy and 14.33±3.20 days of drugs plus surgery. A statistically significant difference was found between the groups (t=8.97, P<0.05). Patients with moderate to severe exposure were healed after 18.19±2.12 days of helium-neon laser therapy and 31.25±4.21 days of drugs plus surgery. The difference between the groups was statistically significant (t=7.91, P<0.05). Enhanced magnetic resonance imaging showed that the helium-neon laser therapy significantly promoted vascularization of the hydroxyapatite orbital implant. These results, combined with pathological findings in animals, which showed that a helium-neon laser promoted vascularization and had anti-inflammatory effects, suggest that helium-neon laser irradiation is an effective method for treating hydroxyapatite orbital implant exposure, thereby avoiding secondary surgery.