AbstractOn 1 October 2021, Bepi‐Colombo performed its first flyby of Mercury. During the maneuver, the short wavelength channel (55–155 nm) of “Probing the Hermean Exosphere by UV Spectroscopy” (PHEBUS) was activated for a total duration of 1 hr. The helium resonance line at 58.4 nm was clearly observed during the whole sequence. At large distance from the planet, the emission was due to helium atoms in the interplanetary medium (interplanetary UV glow). Just after crossing the terminator of the planet and entering the dawn side of the exosphere, PHEBUS observed a clear additional emission due to scattering of solar photons by helium atoms in the exosphere of Mercury. The first detection of the 58.4 nm line in the exosphere of Mercury was reported by Broadfoot et al. (1976, https://doi.org/10.1029/gl003i010p00577) following the Mariner 10 flybys in 1974. The PHEBUS observation of exospheric helium emissions is the first for this element since the UVS measurements. In this paper, we present the results of our analysis of the PHEBUS data at 58.4 nm. Calibration of both instruments are compared with observations of the interplanetary glow, showing that the measurements of both instruments are accurate. However, we find that the exospheric density of helium atoms deduced from the PHEBUS data is 4.5–7.5 times lower than the previous estimate from UVS on Mariner 10. Possible explanations are considered. We show that some of the helium atoms present in the exosphere of Mercury could originate from the local interstellar cloud.