Plant Height Research Articles

Haplotype-based association mapping of genomic regions associated with Zymoseptoria tritici resistance using 217 diverse wheat genotypes

BackgroundSeptoria tritici blotch (STB) is considered to be one of the most destructive foliar wheat diseases and is caused by Zymoseptoria tritici. The yield losses are severe and in Northwestern Europe can reach up to 50%. The efficacy of fungicides is diminishing due to changes in the genetic structure of the pathogen. Therefore, resistance breeding is the most effective strategy of disease management. Recently, genome-wide association studies (GWAS) have become more popular due to their robustness in dissecting complex traits, including STB resistance in wheat. This was made possible by the use of large mapping populations and new sequencing technologies. High-resolution mapping benefits from historical recombination and greater allele numbers in GWAS.ResultsIn our study, 217 wheat genotypes of diverse origin were phenotyped against five Z. tritici isolates (IPO323, IPO88004, IPO92004, IPO86036 and St1-03) and genotyped on the DArTseq platform. In polytunnel tests two disease parameters were evaluated: the percentage of leaf area covered by necrotic lesions (NEC) and the percentage of leaf area covered by lesions bearing pycnidia (PYC). The disease escape parameters heading date (Hd) and plant height (Ht) were also measured. Pearson’s correlation showed a positive effect between disease parameters, providing additional information. The Structure analysis indicated four subpopulations which included from 28 (subpopulation 2) to 79 genotypes (subpopulation 3). All of the subpopulations showed a relatively high degree of admixture, which ranged from 60% of genotypes with less than 80% of proportions of the genome attributed to assigned subpopulation for group 2 to 85% for group 4. Haplotype-based GWAS analysis allowed us to identify 27 haploblocks (HBs) significantly associated with analysed traits with a p-value above the genome-wide significance threshold (5%, which was –log10(p) > 3.64) and spread across the wheat genome. The explained phenotypic variation of identified significant HBs ranged from 0.2% to 21.5%. The results of the analysis showed that four haplotypes (HTs) associated with disease parameters cause a reduction in the level of leaf coverage by necrosis and pycnidia, namely: Chr3A_HB98_HT2, Chr5B_HB47_HT1, Chr7B_HB36_HT1 and Chr5D_HB10_HT3.ConclusionsGWAS analysis enabled us to identify four significant chromosomal regions associated with a reduction in STB disease parameters. The list of valuable HBs and wheat varieties possessing them provides promising material for further molecular analysis of resistance loci and development of breeding programmes.

Effects of Various Salinity and Water Stress Levels on Physiological and Biochemical Traits of Pepper

ABSTRACT Salinity and drought are among the most important abiotic stress factors with vital impacts on plant growth and development. Effects of different salinity and water stress levels on physiological and biochemical traits of pepper were investigated in this study. Five different salinity (S1: 0.3 dS/m; S2: 2.5 dS/m; S3: 5dS/m; S4: 7.5 dS/m; S5: 10 dS/m) and three different irrigation levels (I100: full irrigation; I75: 25% deficit; I50: 50% deficit) were used. Plant water consumption (ET) values decreased in increasing salinity levels. Pepper yield was significantly affected by both salinity and water stress. There was a 62.8% yield loss under salt stress as compared to the control treatment and a 70.1% yield loss under 50% water deficit (I50) as compared to full irrigation (I100). Irrigation water salinity and water deficits caused significant differences in physiological and photosynthetic parameters (plant height, plant fresh and dry weight, leaf area, chlorophyll, and carotenoid content). Salinity and water stress had significant impacts on enzymatic and non-enzymatic contents such as superoxide dismutase (SOD), peroxide (POD), catalase (CAT), proline, malondialdehyde (MDA), total phenolic content (TPC) and total flavonoid content (TFC). It was concluded based on present findings that irrigation waters with a salinity level up to 5 dS/m could be used in pepper cultivation, but water deficits should be avoided.

Formulations of synergistic microbial consortia for enhanced systemic resistance against Fusarium wilt in cumin.

The study aimed to understand the dynamic interplay between plants and their associated microbes to develop an efficient microbial consortium for managing Fusarium wilt of cumin. A total of 601 rhizospheric and endophytic bacteria and fungi were screened for antagonistic activity against Fusarium oxysporum f.sp. cumini (Foc). Subsequently, ten bacteria and ten fungi were selected for characterizing their growth promotion traits and ability to withstand abiotic stress. Furthermore, a pot experiment was conducted to evaluate the bioefficacy of promising biocontrol isolates-1F, 16B, 31B, and 223B in mono and consortium mode, focusing on disease severity, plant growth, and defense responses in cumin challenged with Foc. Promising isolates were identified as Trichoderma atrobruneum 15F, Pseudomonas sp. 2B, Bacillus amyloliquefaciens 9B, and Bacillus velezensis 32B. In planta, results revealed that cumin plants treated with consortia of 15F, 2B, 9B, and 32B showed highest percent disease control (76.35%) in pot experiment. Consortia of biocontrol agents significantly enhanced production of secondary metabolites and activation of antioxidant-defense enzymes compared to individual strain. Moreover, consortium treatments effectively reduced electrolyte leakage over the individual strain and positive control. The four-microbe consortium significantly enhanced chlorophyll (~ 2.74-fold), carotenoid content (~ 2.14-fold), plant height (~ 1.8-fold), dry weight (~ 1.96-fold), and seed yield (~ 19-fold) compared to positive control in pot experiment. Similarly, four microbe consortia showed highest percent disease control (72.2%) over the positive control in field trial. Moreover, plant growth, biomass, yield, and yield attributes of cumin were also significantly increased in field trial over the positive control as well as negative control.

Morphological, anatomical, and transcriptomics analysis reveals the regulatory mechanisms of cassava plant height development

BackgroundCassava is one of three major potato crops and the sixth most important food crop globally. Improving yield remains a primary aim in cassava breeding. Notably, plant height significantly impacts the yield and quality of crops; however, the mechanisms underlying cassava plant height development are yet to be elucidated.ResultsIn this study, we investigated the mechanisms responsible for cassava plant height development using phenotypic, anatomical, and transcriptomic analyses. Phenotypic and anatomical analysis revealed that compared to the high-stem cassava cultivar, the dwarf-stem cassava cultivar exhibited a significant reduction in plant height and a notable increase in internode tissue xylem area. Meanwhile, physiological analysis demonstrated that the lignin content of dwarf cassava was significantly higher than that of high cassava. Notably, transcriptome analysis of internode tissues identified several differentially expressed genes involved in cell wall synthesis and expansion, plant hormone signal transduction, phenylpropanoid biosynthesis, and flavonoid biosynthesis between the two cassava cultivars.ConclusionsOur findings suggest that internode tissue cell division, secondary wall lignification, and hormone-related gene expression play important roles in cassava plant height development. Ultimately, this study provides new insights into the mechanisms of plant height morphogenesis in cassava and identifies candidate regulatory genes associated with plant height that can serve as valuable genetic resources for future crop dwarfing breeding.

Enhancing zinc biofortification and mitigating cadmium toxicity in soil–earthworm–spinach systems using different zinc sources

Cadmium (Cd) pollution poses significant threats to soil organisms and human health by contaminating the food chain. This study aimed to assess the impact of various concentrations (50, 250, and 500 mg·kg–1) of zinc oxide nanoparticles (ZnO NPs), bulk ZnO, and ZnSO4 on morphological changes and toxic effects of Cd in the presence of earthworms and spinach. The results showed that Zn application markedly improved spinach growth parameters (such as fresh weight, plant height, root length, and root-specific surface area) and root morphology while significantly reducing Cd concentration and Cd bioconcentration factors (BCF-Cd) in spinach and earthworms, with ZnO NPs exhibiting the most pronounced effects. Earthworm, spinach root, and shoot Cd concentration decreased by 82.3 %, 77.0 %, and 75.6 %, respectively, compared to CK. Sequential-step extraction (BCR) analysis revealed a shift in soil Cd from stable to available forms, consistent with the available Cd (DTPA-Cd) results. All Zn treatments significantly reduced Cd accumulation, alleviated Cd-induced stress, and promoted spinach growth, with ZnO NPs demonstrating the highest Cd reduction and Zn bioaugmentation efficiencies compared to bulk ZnO and ZnSO4 at equivalent concentrations. Therefore, ZnO NPs offer a safer and more effective option for agricultural production and soil heavy metal pollution management than other Zn fertilizers.

Fusarium oxysporum f. sp. apii race 4 threatening celery production in South Florida.

Fusarium wilt, caused by Fusarium oxysporum f. sp. apii (Foa), constitute a vascular disease affecting celery. This soil-borne pathogen is classified into four distinct pathogenic races: 1, 2, 3, and 4. Notably, race 4 emerges as the most virulent, representing the latest evolutionary development of this pathogen, which was first reported in 2013 in California. In 2022, celery plants in South Florida exhibited typical Fusarium wilt symptoms, with the disease reaching a 100% incidence and causing yield losses ranging from 20% to 100%. Given the significance of celery as a vegetable crop and the severity of this outbreak, the primary objective of this study was to identify and characterize the causal agent of Fusarium wilt in South Florida. The second goal aimed to test the pathogenicity and virulence of the Fusarium isolates from Florida on celery and parsley plants. Using race-specific primers and dual-loci phylogenetic analyses, the isolates surveyed in this study were identified as Foa race 4. Pathogenicity assays in the greenhouse showed that the Foa race 4 isolate from celery induced disease not only on the two celery cultivars (Duda 30 and Duda 71) but also on two commonly cultivated parsley varieties (Curly and Italian). Our study also revealed that Foa race 4 significantly (P < 0.05) affected plant health attributes in all cultivars, including plant height, total plant weight, and root weight. Interestingly, the pathogen exhibited higher (P < 0.0001) virulence on parsley than celery based on vascular discoloration. These findings strongly indicate the urgency of comprehending and managing Fusarium wilt on celery and related crops. Furthermore, the ability of Foa race 4 to affect different plant species highlights a potential threat to agricultural production, emphasizing the need for proactive measures to mitigate the impact of this virulent pathogen.

Assessment of Genetic Variability and Component Relationship of Seed Yield and Its Contributing Traits in Greengram (Vigna radiata L. Wilczek)

After pigeonpea and chickpea, greengram is the third most significant pulse crop in Asia. Pulses are the primary source of protein needed to satisfy both the daily need and the daily consumption for a balanced diet. Pulses are essential to achieving the diet's protein requirements for humans. Because they are the least expensive type of protein, they are referred to as poor people's meat. It is the primary source of vital amino acids for India's vegetarian population. There are twenty genotypes of Greengram in this experiment, plus one check. Three replications of the experiment were carried out at RBD during the month of Zaid in 2023. Thirteen characters' worth of data were collected in order to investigate genetic advancement, variability, heritability, correlation, and path analysis. The majority of the traits under study have significant genetic diversity in the available germplasm, according to analysis of variance. According to the mean performance of grain yield and its constituents, CO-7 performed better than AMULYA, SML-20-10, and IPM-2-14. A detailed examination of the variability coefficients showed that there was no environmental influence on the expression of the traits under study, as evidenced by the small difference between PCV and GCV. For biological yield, number of pods per plant, and number of seed yield per plant, high to moderate estimations of GCV &amp; PCV were noted. For the seed index, plant height, harvesting index, and quantity of seeds per pod, high estimates of heritability were noted. Since they are less affected by environmental factors and have a greater potential for genetic determination, high heritability estimates can be used for genetic improvement. For the seed index, number of branches per plant, and number of clusters per plant, low estimations of genetic advance were noted. According to correlation, plant height, major branches per plant, clusters per plant, pods per plant, biological yield, and harvest index all positively and significantly correlated with grain yield per plant. Path analysis revealed that plant height, cluster size per plant, biological yield, and harvest index have a direct impact on yield at both the phenotypic and genotypic levels. These characteristics are therefore acknowledged as the effective and prospective characteristics for direct selection in order to increase Greengram productivity in the current experimental materials.

Performance of Rosy Adenium (Adenium obesum) Genotype under Prayagraj Agro-Climatic Conditions

Aim: The experiment was conducted in the Department of Horticulture, Sam Higginbottom University of Agriculture Technology and Science, Prayagraj, during 2023-2024. Study Design: The experiment was laid out in Completely Randomized Design (CRD). Methodology: 10 Rosy adenium genotypes and each genotype was replicated 3 times. The different genotypes used in the experiment were R4, R5, R23, R33, R35, R53, R73, R124, R163, R169. Results: The result obtained showed that the genotype R73 showed significantly higher performance in parameters like plant height, caudex diameter, graft length, graft diameter, days taken to bud initiation, number of petals per flower, flower diameter, self-life and which was found to be at par with genotype R33 in plant height, caudex diameter, graft length, graft diameter, days taken to bud initiation, number of petals per flower, flower diameter, self-life. Conclusion: Hence, genotype R73 and R33 is excellent in terms of performance and plant growth under Prayagraj agro-climatic conditions.

Resistances and Physiological Responses of Impatiens uliginosa to Copper Stress

The phytoremediation of soil and water that has been significantly contaminated with metals has potential ecological and economical ramifications, as well as the advantages of high efficiency, and is an environmentally friendly method of ecological pollution control. This study aimed to examine the impact of varying concentrations of Copper (Cu2+) (0, 5, 10, 15, 20, and 25 mg·L−1) on the growth, development, physiology, biochemistry, mineral elements, and features of Cu2+ enrichment of Impatiens uliginosa. This plant is endemic to Yunnan Province in China and is a wetland species. The results showed that the root lengths, stem diameters, plant height, and stem and leaf biomass of I. uliginosa showed a phenomenon of “low promotion and high inhibition,” while the root biomass showed a trend of gradual decreasing. At the early stage of Cu2+ stress (day 6), the activities of peroxidase and catalase and the contents of malondialdehyde (MDA) of I. uliginosa were directly proportional to the concentration of Cu2+. As the treatment time increased, the activation of a defense mechanism in vivo enabled I. uliginosa to adapt to the high Cu2+ environment, and the content of MDA gradually decreased. As the concentration of Cu2+ increased, its contents in the roots, stems, and leaves also gradually increased. In particular, when the concentration of Cu2+ reached 25 mg·L−1, its contents in the roots of I. uliginosa increased by 39.16-fold compared with that of the control group (CK). The concentration-dependent influence of the contents of iron (Fe) and zinc (Zn) in the roots and leaves were observed. Low concentrations of Cu2+ promoted iron content in roots and leaves, and vice versa, while Zn content decreased with the increasing concentration of Cu2+. It was conclusively shown that I. uliginosa has the potential to remediate low concentrations of Cu2+ pollution in water and is a textbook ornamental plant to remediate bodies of water that are polluted with Cu2+.

Relationship between the Orientation of Leaves in Space and the Rice Development Process

The objective of this study was to evaluate plants of SPU-79-96 forms with a short, dense, non-drooping panicle and SPU78-96 plants with a long, drooping panicle. This characteristic of these forms is the vertical arrangement of the leaves on the stem. Methodology and Results: Four growth components were measured after sowing in the field: the angle of deviation of the leaf from the main stem, leaf area, plant height, and length of the main panicle. The experimental design was a randomized split-plot with three (3) replicates. The lowest values were observed in plants SPU-78-96(10,3 to 11,00), highest in SPU-79-96 (10,7 to 12,7 0), the leaf deviation angle in the control variety Nerica 4 was 23,7 to 25,50. The ratio of panicle length to plant height was 23,3 to 29%. Plant yield was higher (1082,4 to 1384,5g/m2) in the long panicle and was 59,13% higher than in the control variety. Conclusion: It can be concluded that the vertical arrangement of the leaves plays a role in the production process, plants with a higher PAR efficiency accumulate more biomass, thus increasing yields.

Exploring population genetics for mutant mungbean [Vigna radiata (L.) R. Wilczek] lines: Insights from augmented block design

AbstractThe current study was initiated to assess mungbean [Vigna radiata (L.) R. Wilczek] mutant lines for yield and their attributes in the mutation generation third (M3) generation using an augmented block design. Note that 1200 mutated mungbean lines were selected from the γ‐irradiated (400–700 Gy) population and distributed into 21 blocks, along with three popular varieties (checks) replicated in each block. To evaluate mean performance, variance, and population genetics, the observations on days to flowering, days to maturity, plant height (cm), number of clusters per plant, number of pods per plant, pod length (cm), protein percentage, seed index, and seed yield (g) were measured and analyzed. All the lines under consideration showed highly significant variations for all the variables, except for days to flowering. Seventy‐nine mutant lines were found to have significantly better yield attributes than checks and are currently being evaluated under station trial. Skewness and kurtosis analysis unveiled the presence of gene interactions, offering opportunities for targeted improvement and efficacy of γ rays as a mutagen, facilitating the release of variability within the population. Future mungbean breeding programs will benefit from the successful isolation of mutant plants with yield‐enhancing traits, such as up to 20 clusters per plant, 71 pods per plant, 8.55 cm pod length, 26.69% protein content (5% higher than parent), and 29 gram seeds per plant. In early selection cycles without the need for replicated trials, this study demonstrated the effectiveness of the mutagen and augmented design in creating novel variations, evaluating and identifying superior genotypes with improved yield potential.

Performance of Different Aglaonema (Aglaonema commutatum) Varieties under Prayagraj Agro-climatic Conditions

The present investigation was undertaken in the Department of Horticulture, Sam Higginbottom University of Agriculture, Technology And Sciences, Prayagraj, during August, 2023 to April, 2024. The experiment was laid out in completely randomized design with eight different aglaonema varieties viz., Dove, Earnest Round Leaf, Watson Dwarf, Butterfly, Ice, Red Lipstick, Nitidum and Rotundum, replicated thrice and carried out under 50% shade net conditions. Variety Butterfly reported significantly better performance compared to other varieties in terms of growth parameters like plant height (48.6 cm), number of leaves (5.4), plant spread (31.2 cm2), stem girth (5.7 cm), leaf area (129.6 cm2), minimum leaf production interval (12.6 days), number of new sprouts (7), chlorophyll content (21.6), plant growth index based on height of the plants (7.5), plant growth index based on number of leaves (12.6) and survival percentage (100%).

Assessment of Genetic Variability, Correlation, and Path Coefficient for Yield and Its Contributing Traits in Pigeon Pea [Cajanus cajan (L.) Millspaugh

The experimental material consisted of 155 pigeon pea genotypes sown in a Randomized Block Design (RBD) with three replications during the Kharif, 2023-2024 crop season at the N. E. B. Crop Research Centre of G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand. The genetic variance components, correlation, and path coefficient for seed yield and its components were estimated using these 155 pigeon pea genotypes. The highest phenotypic coefficient of variation (PCV) values (&gt; 20%) were recorded for traits such as the number of pods per plant (26.45%), number of secondary branches per plant (22.72%), and seed yield per plant (20.27%). In contrast, the lowest PCV estimates (&lt; 10%) were found for days to maturity (7.39%) and days to 50% flowering (6.73%). High estimates of the genotypic coefficient of variation (GCV) (&gt; 20%) were recorded only for the number of pods per plant (23.6%). The lowest GCV estimates (&lt; 10%) were observed for hundred seed weight (9.63%), number of seeds per pod (9.55%), plant height (9.49%), pod length (7.18%), days to maturity (6.85%), and days to 50% flowering (6.5%). The hundred seed weight showed a high positive direct effect on seed yield per plant (Genotypic = 0.58, Phenotypic = 0.47) along with a positive and significant correlation (Genotypic correlation coefficient, (rg = 0.599); Phenotypic correlation coefficient, (rp = 0.485)). Based on this investigation, it is evident that exploring genetic variability, correlations, and path analyses provides a more effective approach for selecting superior cultivars for yield and related traits.

Effect of Different Levels of NPK, Vermicompost and Sulphur on Growth, Yield and Economics Attributes of Mustard (Brassica juncea L.) var. Giriraj

A field experiment was conducted at Research farm, Department of Soil Science and Agricultural Chemistry, Naini Agricultural Institute, SHUATS, Prayagraj (U.P.) during the Rabi season of 2023-24 with the objective to differentiate levels of NPK, Vermicompost and Sulphur on yield attributes of Mustard (Brassica juncea L.) var. Giriraj. The experiment was laid out in a Randomized Block Design with nine treatment combinations, consisting of three N, P and K levels (0, 50 and 100%), Vermicompost (0, 50 and 100%) and Sulphur (0, 50 and 100%). It was observed that the treatment of the application of 100% @ (NPK) +100% @ VC + 100 % @ S (T9) was best in terms of growth parameters in maximum plant height (124.01 cm), Number of branches (12.28), No. of leaves (75.96), number of silique plant-1 (168.67), test weight (4.24) and total yield was highest i.e. (19.90 qha-1).

The effect of biostimulants and red mud on the growth and yield of shallots in post-unlicensed gold mining soil

Abstract Shallot production in West Kalimantan is still low. Land that has the potential for the development of shallots is the soil from post-unlicensed gold mining (PUGM). The soil at PUGM was exploited very extensively and can be used to develop shallots, but it is obstacled by low pH values and nutrient content. This research aims to investigate the impact of biostimulants and red mud on the growth and yield of shallots. The experiment was carried out in two stages: (1) investigated the ratio of the red mud and PUGM soil at the laboratory and (2) investigated the biostimulants and red mud effect on two varieties of shallots in a screen house. For the second experiment, there were three factors. The first factor was the biostimulant (0, 2, 3, and 4%); the second factor was the dose of red mud (0, 2, and 4 tons ha−1); and the third factor was the shallot varieties (Bima Brebes and Tajuk). The results of the first research indicated that the application of the red mud and PUGM soil in a ratio of 1:500 and 1:1,000 had a pH of 7.88 (slightly alkaline) and 7.24 (neutral), respectively. The second study found that a 2% concentration of biostimulant increased plant height and tuber count by 23.78 cm and 6.55 tubers, respectively. The Bima Brebes variety produced better fresh tubers weight clump−1 and dry tubers weight clump−1 than the Tajuk variety, with, respectively, 42.08 g (7.36 tons ha−1) and 32.60 g (5.71 tons ha−1). These findings show that the use of the biostimulants and red mud together in integrated management can help shallots grow and produce more, especially on PUGM land that has obstacles with low soil fertility. This research contributes to the identification of sustainable solutions for agricultural development in environments that have experienced severe land degradation, such as unlicensed gold mining activities.

Effect of Transient Waterlogging Stress on Growth, Physiology and Yield of Cowpea (Vigna unguiculata L.) in Semi-Arid Region

Cowpea cultivation during rainy season is highly affected by the waterlogging stress due to unpredicted high-intensity rains. The studies on assessment of waterlogging effect on different growth stages of cowpea are necessary for planning mitigation strategies. Hence study was conducted during kharif (June to September) 2022 under factorial randomized block design (FRCBD) set up. The first factor consisted of seven waterlogging durations (3 to 15 days), and second factor was three growth stages of cowpea (15 DAE; Days after emergence, 25 DAE and at 50% flowering). The results revealed that regardless of growth stages, growth and yield attributes were drastically decreased with increased duration of waterlogging. The highest plant height (25.07 cm), number of branches plant–1 (5.33) and leaf area (205.27 cm2 plant–1), and number of pods plant–1 (4.27), pod length (15.24 cm), number of seeds pod–1 (14.27), grain (6.27 g plant–1) and haulm yield (15.62 g plant–1) were recorded with 3 days of waterlogging, whereas lowest values were reported with 15 days of waterlogging. Regarding growth stages, highest growth, and yield attributes were recorded with waterlogging during 50% flowering, followed by 25 DAE and 15 DAE. Moreover, the correlation study indicated that physiological parameters such as leaf protein content (r = 0.95) and Normalized Difference Vegetation Index NDVI (r = 0.97) were positively related to grain yield. It was found that, cowpea is sensitive to high-intensity waterlogging (beyond 3–5 days) especially during the early growth stage (15 DAE).

Induced genetic diversity through mutagenesis in wheat gene pool and significant use of SCoT markers to underpin key agronomic traits

BackgroundThis research explores the efficacy of mutagenesis, specifically using sodium azide (SA) and hydrazine hydrate (HZ) treatments, to introduce genetic diversity and enhance traits in three wheat (Triticum aestivum L.) genotypes. The experiment entails subjecting the seeds to different doses of SA and HZ and cultivating them in the field for two consecutive generations: M1 (first generation) and M2 (second generation). We then employed selective breeding techniques with Start Codon Targeted (SCoT) markers to select traits within the wheat gene pool. Also, the correlation between SCoT markers and specific agronomic traits provides insights into the genetic mechanisms underlying mutagenesis-induced changes in wheat.ResultsIn the study, eleven genotypes were derived from parent varieties Sids1, Sids12, and Giza 168, and eight mutant genotypes were selected from the M1 generation and further cultivated to establish the M2 generation. The results revealed that various morphological and agronomical characteristics, such as plant height, spikes per plant, spike length, spikelet per spike, grains per spikelet, and 100-grain weight, showed increases in different genotypes from M1 to M2. SCoT markers were employed to assess genetic diversity among the eleven genotypes. The bioinformatics analysis identified a correlation between SCoT markers and the transcription factors ABSCISIC ACID INSENSITIVE3 (ABI3) and VIVIPAROUS1 (VP1), crucial for plant development, growth, and stress adaptation. A comprehensive examination of genetic distance and the function identification of gene-associated SCoT markers may provide valuable insights into the mechanisms by which SA and HZ act as mutagens, enhancing wheat agronomic qualities.ConclusionsThis study demonstrates the effective use of SA and HZ treatments to induce gene diversity through mutagenesis in the wheat gene pool, resulting in the enhancement of agronomic traits, as revealed by SCoT markers. The significant improvements in morphological and agronomical characteristics highlight the potential of mutagenesis techniques for crop improvement. These findings offer valuable information for breeders to develop effective breeding programs to enhance wheat quality and resilience through increased genetic diversity.

Once in Three-Year Deep-Banding and Annual Shallow-Banding of P, K and Cu Fertilizer Effects on Crops and Soil Nutrients

Deep-banding of the immobile nutrients (P, K, and Cu) is considered one technique that may improve crop production under temperate dryland systems, where moister soil conditions prevail at seeding followed by relatively drier soil during the growing period. The objectives were to compare growth and yield of canola (Brassica napus L.), field pea&amp;rsquo;s (Pisum sativum L.) and wheat (Triticum aestivum L.); and nutrient concentrations in crops and soil from once in three-year deep-banding (12.5-15 cm) and annual shallow-banding (3.75 to 5 cm diagonally deeper than seeding depth) of P, K and Cu under direct seeding systems. Small plot trials were conducted at the three sites; representing Brown (Dark Brown Chernozem), Black (Black Chernozem), and Grey (Dark Grey Luvisol) soil zones in Alberta, Canada; which represent crop growing conditions for most areas of western Canada. The nine treatments were annual shallow-banding and once in three year deep-banding of P, K, Cu and PKCU nutrients plus a control (no P, K, or Cu fertilizer). Applied thrice the recommended rate for deep-banding (in 2018) treatments and the recommended rate for the annual shallow-banding treatments in 2018, 2019 and 2020. All three phases of pea&amp;rsquo;s-wheat-canola rotation were grown at each site to generate nine years of data for each crop. The density, height, NDVI (normalized difference vegetation index) and biomass of plants, yield for crops and nutrient concentrations in seed and biomass of crops and soil showed significantly positive responses to P, K, and PKCu additions in some cases; generally not linked to the deep or shallow placement of tested nutrients. Changes in nutrient concentrations in seed and biomass of crops in response to nutrient additions were not consistent. Treatments with P showed response more often than with the K and Cu. The soil P and Cu concentrations increased in response to their additions in some cases, but no change was observed from the K addition. Once in three-year deep-banding or annual shallow-banding methods were equally effective. The findings provide another option (once in three year deep-banding) for applying the P, K and Cu fertilizers.

Manifestation of the Effect of Growth Heterosis in Intraspecific Hybrids of Soft Wheat

The presented article presents the results of the study of height inheritance in hybrid combinations of the first generation (F1) of bread wheat. The research was conducted in the 2019-2020 growing season at the Apsheron District Experimental Farm under irrigation conditions. In 32 combinations of hybrids of the first generation (F1), obtained as a result of reciprocal crossing of 9 local varieties («Mirbashir-128», «Azeri», «Gobustan», «Fatima», «Gyrmyzy gul-1», «Murov-2», «Askeran», «Matin» and «Onur»). Studied the height of plants in comparison with parental forms and determined the degree of true heterosis and dominance. In the studied hybrid combinations, a variety from high dominance to depression was found in the transmission of heredity. When analyzing hybrids of bread wheat of the first generation (F1) according to the inheritance of plant height, high dominance was determined in 19 combinations (59.4%), depression — in 8 combinations (25.0%), intermediate heredity - in 5 combinations (15.6% ). Positive heterosis was revealed in 19 combinations (59.4%), negative heterosis - in 13 combinations (40.6%). As a result of the research, it is possible to initially conclude that it is possible to obtain low-growing hybrids by involving in the hybridization a variety of the same height.

Influence of Urea Foliar Nutrition and Leaf Harvesting Intensity on Growth, Yield Attributes and Yield in Cowpea [Vigna unguiculta (L.) Walp.

A field experiment was undertaken at Farming Systems Research Station (FSRS), Sadanandapuram, Kollam district, Kerala Agricultural University in summer 2022 for studying the effect of growth, yield attributes and yield of cowpea through foliar application of 2% urea and intensity of leaf harvesting. Experimental treatments comprised of 4 levels of foliar nutrition management (2% urea spray at vegetative stage (f1), 2% urea spray at flowering stage (f2), 2% urea spray at vegetative stage and flowering stage (f3), water spray control (f4) and 3 levels of leaf harvest (20% leaf harvest (l1), 40% leaf harvest (l2), and no leaf harvest (l3). Among the foliar nutrition management treatments, f3 showed significantly the taller plants (91.97 cm), more number of branches (15.13), leaves (34.76), leaf area/ plant (884.29 cm2), leaf area index (1.96) dry matter production (42.39 g) and chlorophyll content (3.544 mg/g) and were comparable with f1. The nodule parameters such as nodule number (37.44) and effective number of nodules (32.11) were the highest in treatment f3 and was on par with f1. Further, days to 50 per cent flowering (53.65 days), pod length (17.27 cm), 100 seed weight (12.40 g), pod number (24.58), foliage yield (1484.57 kg ha-1), grain yield (925.85 kg ha-1) and haulm yield (2722.70 kg ha-1) were the highest in treatment f3 and was on par with f1 except for pod weight (21.19 g). Among the leaf harvest intensity, treatment l3 recorded the taller plants (92.45 cm), leaf area per plant (902.71cm2) leaf area index (2.01), less days to 50% flowering (47.60 days) and haulm yield (2754.85 kg ha-1). It was on par with l1 pertaining to plant height. The number of branches (15.03), dry matter production per plant (40.78g), pod weight/plant (19.43 g), pod number/plant (23.59) and grain yield (925.85 kg ha-1) were the highest in treatment l1.