Hedychium Species Research Articles

Pharmacological Activities of Six Species of Hedychium J. Koenig from Nepal

Species of Hedychium Koenig are perennial herbs. Some of the species like H. ellipticum, H. spicatum, H. coronarium are traditionally used as medicinal plants. In the present study, methanolic rhizome extracts of six different species of Hedychium namely H. spicatum, H. ellipticum, H. thyrsiforme, H. coccineum, H. gardnerianum and H. coronarium were analysed for their antioxidant, antidiabetic and antibacterial activities. The antioxidant activity analysed by DPPH assay showed the highest potential (lowest IC50 value) in H. coccineum (148.82±2.83 µg/ml) and lowest potential (maximum IC50 value) in H. thyrsiforme (996.55±9.42 µg/ml). The rhizome extracts of different species showed moderate α-amylase inhibition activity in vitro. The highest α-amylase inhibition (79.67%) was observed for H. coronarium while the lowest inhibition (64.0%) was observed in H. thyrsiforme. However, these values were found lower than the value (92.37%) obtained for positive control, i.e., Acarbose. The antibacterial activity was determined against two Gram-positive (Bacillus subtilis and Staphylococcus aureus) and two Gram-negative (Klebsiella pneumoniae and Pseudomonas aeruginosa) bacterial strains by agar well diffusion method. Except for H. ellipticum the extracts of all other species showed antibacterial activity against all the bacterial strains tested. The extracts of H. ellipticum showed antibacterial activity only against B. subtilis and K. pneumoniae. The extract of H. coronarium showed the highest zone of inhibition (16.67±1.15 mm) against B. subtilis. However, the antibacterial activity was weak compared to standard antibiotics for all the extracts and at all concentration tested. These results show that rhizomes of other species can also be used in the same manner as that of H. coronarium and H. spicatum, two species most used in various ethnomedicinal applications.

Chemical Composition, Toxicity, and Repellency of Essential Oils from Three Hedychium Species Against Stored-Product Insects.

Stored products are constantly infested by insects, so finding eco-friendly bioinsecticides for insect management is important. The work aimed to assess the insecticidal and repellent activity of essential oil (EO) from Hedychium glabrum S. Q. Tong, Hedychium coronarium Koen., and Hedychium yunnanense Gagnep. against Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila. Results showed that 88 chemical components were identified in the extracted Hedychium EOs, indicating that they exhibited diversity in components. According to principal component analysis (PCA), the composition of the EO from the H. yunnanense stem and leaf (EOHYSL) was significantly different from other EOs due to the different organs and species. The biological activity also varied continuously with plant species and organs. Only the EO of H. yunnanense (EOHY) showed strong fumigant toxicity. While in the contact tests, EOHGR showed the strongest toxicity effect on L. bostrychophila, with a LC50 value of 71.76 μg/cm2, which was closest to the positive control (Pyrethrin). All EOs had remarkable repellent activities against the three target insects, and repellency increased with concentration. According to the results of the comprehensive score, EOHY had the highest potential, which ranged from 0.7999 to 0.8689. Thus, Hedychium EOs possess potential biorational traits to be biological insecticides.

In-vitro, in-vivo and in-silico anti-inflammatory evaluation of hedychenone and its semisynthetic derivative

Hedychium spicatum is a perennial shrub that is commonly found in the Himalayan region. Thin layer chromatography fingerprint screening of various Hedychium species revealed that only the species Hedychium spicatum possesses hedychenone. Purification and isolation of hedychenone was carried out from methanolic extract of Hedychium spicatum rhizomes powder using column chromatography. After crystallization of enriched fraction, 2.19% of hedychenone crystalline powder was yielded. The physiochemical, pharmacokinetic, and pharmacodynamic evaluation of the active pharmaceutical components was conducted using artificial learning and machine learning (AL/ML) approaches. The results indicate that both medications adhere to Lipinski’s rule of five. The docking study indicated that compound 2 (MBE-10.4 kcal/mol) exhibited a more sub-stantial interaction with the cannabinoid receptor 2 compared to hedychenone (MBE -9.85 kcal/mol). A study was done to assess the physical and chemical stability of hedychenone under biological pH conditions. Furthermore, the administration of both medication concentrations, 100 mg/kg and 200 mg/ kg, effectively inhibits the development of acute paw edema caused by ovalbumin. A preliminary investigation of hedychenone and its synthetic derivative indicated that these compounds have the potential to be utilized as active medicinal ingredients in herbal pharmaceutical preparations.

Variation in the Chemical Composition of Endemic Specimens of Hedychium coronarium J. Koenig from the Amazon and In Silico Investigation of the ADME/Tox Properties of the Major Compounds.

Four species of the genus Hedychium can be found in Brazil. Hedychium coronarium is a species endemic to India and Brazil. In this paper, we collected six specimens of H. coronarium for evaluation of their volatile chemical profiles. For this, the essential oils of these specimens were extracted using hydrodistillation from plant samples collected in the state of Pará, Brazil, belonging to the Amazon region in the north of the country. Substance compounds were identified with GC/MS. The most abundant constituent identified in the rhizome and root oils was 1,8-cineole (rhizome: 35.0-66.1%; root: 19.6-20.8%). Leaf blade oil was rich in β-pinene (31.6%) and (E)-caryophyllene (31.6%). The results from this paper allow for greater knowledge about the volatile chemical profile of H. coronarium specimens, in addition to disseminating knowledge about the volatile compounds present in plant species in the Amazon region.

Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India

Ethnopharmacological relevanceThe genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours. Several species of Hedychium have remained under-explored with respect to their chemical composition and biological activities. Aim of the studyThe current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum). Materials and methodsCompound identification was accomplished using a Clarus 580 gas chromatography system in conjunction with mass spectrometry (GC-MS). The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes. MTT assay was employed to visualize the antiproliferative property against MCF7, HepG2 and PC3 cancerous cell lines. The toxicity of essential oils was determined using 3T3-L1 non-tumorigenic/normal cells. Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method. Furthermore, the levels of prostaglandin (PGE2) and pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) was assessed using the ELISA technique. ResultsIn total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents. Monoterpenes (8.5–59.9%) and sesquiterpenes (2.87–54.11%) were the two class of compounds, found as the most prevalent in the extracted essential oils. The multivariate analysis classified the four Hedychium species into three different clusters. Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited the highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively. The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects. Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group. ConclusionThe present findings revealed that the Hedychium species traditionally used in therapeutics could be a potential source of active compounds with antiproliferative and anti-inflammatory properties.

A Comprehensive Review on Pharmacologically Active Phyto-Constituents from Hedychium species.

In this review, we describe and discuss the phytoconstituents present in Hedychium species and emphasize their potential as drug candidates. Though they are widely validated in vitro and in vivo models, to date, no efforts have been made to compile in a single review all the pharmacologically active phytoconstituents from Hedychium species, and their pharmacological and toxicity profile. In this study, we present a reinvestigation of the chemical constituents present in Hedychium species obtained from the essential oil and solvent extraction of the flowers, leaves and rhizomes under consideration. Key databases such as PubMed, Science Direct, Scopus, and Google Scholar amongst others were probed for a systematic search using keywords to retrieve relevant publications on this plant. An exhaustive electronic survey of the related literature on Hedychium species resulted in around 200 articles. Articles published between the years 1975-2021 were included. The studies conducted on either crude extracts, solvent fractions or isolated pure compounds from Hedychium species reported with a varied range of biological effects such as anti-inflammatory, analgesic, antidiabetic, potentially anti-asthmatic, and cytotoxic, among other related activities of the chemical constituents present in its essential oil and solvent extract deployed in this review. Traditional and herbal medication around the world that uses different parts of Hedychium species were considered for anti-inflammatory, skincare, analgesic, anti-asthmatic, anti-diabetic, antidotal uses, among others. These uses support the idea that chemical constituents obtained from solvent extraction may also exert the same action individually or in a synergistic manner. The review concluded that there is scope for computation and biological study to find out possible new targets for strengthening the potency and selectivity of the relevant compounds, and to find a commercial method for extraction of active pharmaceutical ingredients.

A comparative study of the volatiles emitted by flowers of three Hedychium species from La Réunion

ABSTRACT With the objective to develop a scent database that could be used for perfume and cosmetics applications, a comparative analysis of the floral profiles close to one hundred odorous flowering plants found in Reunion Island has been undertaken for the last few years in our laboratory. A part of this work relating to three Hedychium species (the well-known H. coronarium but also H. flavescens and H. gardnerianum) is presented here. To achieve this, the solid-phase microextraction (SPME) on living flowers coupled to GC/MS was used. A total of 53 volatiles were identified belonging to aliphatic ketones, monoterpenes (hydrocarbons and oxygenated), sesquiterpenes (hydrocarbons and oxygenated), phenylpropanoids/benzenoids and miscellaneous. The phenylpropanoid/benzenoid and terpenoid classes were the main volatile classes. (E)-β-ocimene, linalool, (E,E)-α-farnesene, (E)-nerolidol, methyl benzoate, methyl salicylate, (E)-isoeugenol and indole were the major compounds detected. Results also revealed significant aroma variation among the different flowers.

A comparative study of the volatiles emitted by flowers of three Hedychium species from La Réunion

A comparative study of the volatiles emitted by flowers of three Hedychium species from La Réunion

Shoot multiplication of three Hedychium species via immersion temporary bioreactor

ISHS IX International Scientific and Practical Conference on Biotechnology as an Instrument for Plant Biodiversity Conservation (physiological, biochemical, embryological, genetic and legal aspects) Shoot multiplication of three Hedychium species via immersion temporary bioreactor

Essential oil composition of Hedychium species from India

Essential oil composition of Hedychium species from India

Coordinated and High-Level Expression of Biosynthetic Pathway Genes Is Responsible for the Production of a Major Floral Scent Compound Methyl Benzoate in Hedychium coronarium.

Methyl benzoate is a constituent of floral scent profile of many flowering plants. However, its biosynthesis, particularly in monocots, is scarcely reported. The monocot Hedychium coronarium is a popular ornamental plant in tropical and subtropical regions partly for its intense and inviting fragrance, which is mainly determined by methyl benzoate and monoterpenes. Interestingly, several related Hedychium species lack floral scent. Here, we studied the molecular mechanism of methyl benzoate biosynthesis in H. coronarium. The emission of methyl benzoate in H. coronarium was found to be flower-specific and developmentally regulated. As such, seven candidate genes associated with methyl benzoate biosynthesis were identified from flower transcriptome of H. coronarium and isolated. Among them, HcBSMT1 and HcBSMT2 were demonstrated to catalyze the methylation of benzoic acid and salicylic acid to form methyl benzoate and methyl salicylate, respectively. Methyl salicylate is a minor constituent of H. coronarium floral scent. Kinetic analysis revealed that HcBSMT2 exhibits a 16.6-fold lower Km value for benzoic acid than HcBSMT1, indicating its dominant role for floral methyl benzoate formation. The seven genes associated with methyl benzoate biosynthesis exhibited flower-specific or flower-preferential expression that was developmentally regulated. The gene expression and correlation analysis suggests that HcCNL and HcBSMT2 play critical roles in the regulation of methyl benzoate biosynthesis. Comparison of emission and gene expression among four Hedychium species suggested that coordinated and high-level expression of biosynthetic pathway genes is responsible for the massive emission of floral methyl benzoate in H. coronarium. Our results provide new insights into the molecular mechanism for methyl benzoate biosynthesis in monocots and identify useful molecular targets for genetic modification of scent-related traits in Hedychium.

Differential expression and functional analysis of two short-chain alcohol dehydrogenases/reductases in Hedychium coronarium

Abstract In this study, the full cDNA sequences of HcADH2 and HcADH3 were cloned from Hedychium coronarium. The amino acid sequences encoded by them contained three most conserved motifs of short-chain alcohol dehydrogenase (ADH), namely NAD+ binding domain, TGxxx[AG]xG and active site YxxxK. The highest similarity between two genes and ADH from other plants was 70%. Phylogenetic analysis showed that they belonged to a member of the short-chain dehydrogenases/reductases 110C subfamily, but they were distinctly clustered in different clades. Real-time polymerase chain reaction analyses showed that HcADH2 was specifically expressed in bract, and it was expressed higher in no-scented Hedychium forrestii than other Hedychium species, but was undetectable in Hedychium coccineum. HcADH3 was expressed higher in the lateral petal of the flower than in other vegetative organs, and it was expressed the most in H. coronarium that is the most scented among Hedychium species, and its expression levels peaked at the half opening stage. HcADH2 and HcADH3 had almost no significant expression in leaves, but HcADH2 was expressed in response to external stimuli. The mechanical injury and methyl jasmonate (MeJA) treatment could induce expression of HcADH2 in leaves, whereas HcADH3 could have an induced expression only by MeJA. The recombinant HcADH3 protein, but not HcADH2, expressed in Escherichia coli-catalysed conversion of geraniol into citral. It was speculated that HcADH3 had an induced expression in vegetative organ of H. coronarium and took part in monoterpenoid biosynthesis in H. coronarium flowers, but the role of HcADH2 is relevant only for defensive reactions.

Uncharted Source of Medicinal Products: The Case of the Hedychium Genus.

A current research topic of great interest is the study of the therapeutic properties of plants and of their bioactive secondary metabolites. Plants have been used to treat all types of health problems from allergies to cancer, in addition to their use in the perfumery industry and as food. Hedychium species are among those plants used in folk medicine in several countries and several works have been reported to verify if and how effectively these plants exert the effects reported in folk medicine, studying their essential oils, extracts and pure secondary metabolites. Hedychium coronarium and Hedychium spicatum are the most studied species. Interesting compounds have been identified like coronarin D, which possesses antibacterial, antifungal and antitumor activities, as well as isocoronarin D, linalool and villosin that exhibit better cytotoxicity towards tumor cell lines than the reference compounds used, with villosin not affecting the non-tumor cell line. Linalool and α-pinene are the most active compounds found in Hedychium essential oils, while β-pinene is identified as the most widespread compound, being reported in 12 different Hedychium species. Since only some Hedychium species have been investigated, this review hopes to shed some light on the uncharted territory that is the Hedychium genus.

EKSPLORASI JENIS – JENIS ZINGIBERACEAE DI CAGAR ALAM RAYA PASI GUNUNG POTENG KOTA SINGKAWANG KALIMANTAN BARAT

Pasi Nature Reserve of Poteng Mount includes tropical forest type and has many biological diversities, one of them is Zingiberaceae family. Research on the potential of Zingiberaceae in Nature Reserve Poteng Mount Pasi area has never been done, therefore it is necessary to research the types of Zingiberaceae species available in this location. The purpose of this research is to know the types of zingiberaceae in Mount Poteng, and its benefits can provide data and information about the types of Zingiberaceae. This research was conducted on December 23, 2016. The method used is the exploration method and the collection of flora, by exploring every angle of the study sites that can represent the types of habitat or vegetation in the area. The results are 9 species of 7 genus with a total of 37 individuals were found. 1 species of genus Alpinia, 1 species of genus Etlingera, 1 species of genus Globa, 1 species of genus Hedychium, 2 species of genus Hornstedtia, 1 species of genus Scaphoclamys and 2 species of genus Zingiber.Keywords: Exploration, Mount Poteng, Zingiberaceae

The Ginger Prophecy; A Review of the Underexplored Genus, Hedychium against Cancer

Cancer, the seventh most fatal disease in the world, poses a long struggle to combat this deadly disease. Economically and pharmacologically important members of Zingiberaceae plant family have helped to eradicate a number of ailments worldwide. This review highlights gingers and their wide spectrum of medicinal values focussing on their role as anticancer agents with facts and data obtained from literature review performed using PubMed, PMC, ScienceDirect, Google Scholar in a systematic way. Among the many genera underlined in this review, several species of Hedychium have emerged as potential cancer treatments with remarkable activity against different forms of tumour. However this genus is not much explored to unravel its value to medical research. Several species have been found to have cytotoxic, antiinflammatory, antioxidant and antitumour activities but not many attempts made to establish the plant principles as anticancer agents.

Chromosomal and PCR-based molecular characterization of Hedychium spp. of Tripura, North-East India

Hedychium is an important genus of Zingiberaceae because of its medicinal and horticultural importance. Investigations on chromosomal and polymerase chain reaction (PCR) based molecular characterization of the genus Hedychium at inter-specific and intra-specific levels have been undertaken for the detailed study of the karyotypes as well as for assessing the genetic diversity. We have analysed chromosomal and molecular marker based characters of three Hedychium spp., viz., H. coccineum, H. coronarium and H. thyrsiforme from the state of Tripura but because of their restricted distributions in this biodiversity hotspot we included only two populations of each species in intra-species analysis. Numerical data of karyotypes reveal that Pop-I and Pop-II of all the three species have the same chromosome count 2n = 34 with a basic number X = 17. In-spite of the close resemblances of their karyotypes, chromosomal data based clustering pattern and their sub-grouping at intra-species level validates the taxonomic status of the species. The inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) dendrogram obtained by unweighted pair group method with arithmetic mean (UPGMA) analysis depicts a local position effect based clustering pattern with intra-specific diversity. Our results also indicate that SSR marker is more reliable in assessing the genetic relationship between and within the species of Hedychium. As Gower’s similarity coefficient is an indicator of cryptic changes in chromosome structure, we propose that dissimilarity in mean centromeric asymmetry (MCA) value at inter-specific level can be related with molecular markers based genetic diversity having high degree of polymorphism.

A Hedychium coronarium short chain alcohol dehydrogenase is a player in allo-ocimene biosynthesis.

An enzyme is crucial for the formation of Hedychium coronarium scent and defense responses, which may be responsible for the biosynthesis of allo-ocimene in H. coronarium. Hedychium coronarium can emit a strong scent as its main scent constituents are monoterpenes and their derivatives. Among these derivatives, allo-ocimene is not only a very important volatile substance in flower aroma, but is also crucial to plant defense. However, the molecular mechanism of allo-ocimene biosynthesis has not been characterized in plants. In this study, a new alcohol dehydrogenase gene, HcADH, was cloned. The amino acid sequences encoded by HcADH contained the most conserved motifs of short chain alcohol dehydrogenase/reductases (SDRs), which included NAD+ binding domain, TGxxx[AG]xG and active site YxxxK. Real-time PCR analyses showed that the HcADH was highly expressed in the outer labellum but was almost undetectable in vegetative organs. The change in its expression level in petals was positively correlated with the emission pattern of allo-ocimene during flower development. HcADH expression coincides also the release level of allo-ocimene among different Hedychium species. Although HcADH is not expressed in the leaves, HcADH expression and allo-ocimene release in leaves can be induced by mechanical wounding or methyl jasmonate (MeJA) treatment. In addition, the expression of HcADH induced by mechanical wounding can be prevented by acetylsalicylic acid, a jasmonic acid biosynthesis inhibitor, suggesting that jasmonic acid might participate in the transmission of wounding signals. Using the Barley stripe mosaic virus (BSMV)-VIGS method, it was found that BSMV:HcADH335 inoculation was able to down-regulate HcADH expression, decreasing only the release of allo-ocimene in flowers while the content of other volatile substances did not decrese. In vitro characterization showed that recombinant HcADH can catalyze geraniol into citral, and citral is an intermediate of allo-ocimene biosynthesis. HcADH may be responsible for the biosynthesis of allo-ocimene in H. coronarium, which is crucial for the formation of H. coronarium scent and defense function.

Hedychiumziroense (Zingiberaceae), a new species of ginger lily from Northeast India.

We describe Hedychiumziroensesp. nov. from Northeast India (NE India) which was discovered during one of our recent botanical explorations in Arunachal Pradesh. We provide detailed morphological comparison of this species with four other Hedychium species (H.griersonianum R.M.Sm., H.ellipticum Buch.-Ham. ex Sm., H.gomezianum Wall. and H.yunnanense Gagnep.), with which it shares some morphological similarities. The new species is characterised by a dense cylindrical spike, pubescent rachis, folded bracts, 2–3 flowers per cincinnus, deeply cleft labellum and a distinctive late monsoonal flowering phenology from August to September.

Volatile metabolite profiling of ten Hedychium species by gas chromatography mass spectrometry coupled to chemometrics

The Hedychium genus (Zingiberaceae) produces a plethora of secondary metabolites that include terpenoids, some of which are known for their aromatic properties. The volatile compositions of several members of this genus have not been chemically defined, as many species are endemic to remote ecosystems. In the present research, the essential oil composition of ten Hedychium species (H. gardnerianum, H. flavescens, H. thyrisiforme, H. flavum, H. ellipticum, H. aurantiacum, H. gracile, H. greenii, H. spicatum, and H. coronarium) was studied by gas chromatography flame ionization detector (GC-FID) and gas chromatography mass spectrometry (GCMS). The essential oil yield obtained by hydrodistillation varied from 0.05 (%v/w) in H. greenii to 0.75 (%v/w) in H. ellipticum and H. thyrsiforme, respectively. A total of 56 constituents representing 75.93–99.66% of volatile constituents were characterized in the essential oil of 10 Hedychium species. The predominant components were β-pinene in H. gardnerianum, H. gracile, H. thyrsiforme, H. flavum and H. flavescens; bornyl acetate in H. greenii; humulene epoxide I in H. aurantiacum; 1,8-cineole in H. spicatum and H. coronarium. Agglomerative Hierarchical Clustering (AHC) and Principal component analysis (PCA) based on essential oil compositions grouped Hedychium species into three different clusters. In view of the ambiguity existing among different Hedychium species, the developed chemical fingerprint in the present study would assume great significance in identification and classification of Hedychium species.

Distributional note on two Hedychium Species from Nagaland, India

Two Hedychium species Hedychium densiflorum Wall. and Hedychium wardii C.E.C. Fisch. have been collected for the first time from the state of Nagaland, India. Detailed descriptions of the species along with colour photographs have been provided for their identification and reference.