Small indigenous fish (SIF) are recognized as easily available, and abundant source of macronutrients and micronutrients in Bangladesh. The current investigation was undertaken to assess the existing heavy metal, mineral, vitamin, and others nutrient content of small indigenous freshwater fish in Bangladesh. Eight indigenous fish (Mola, Tengra, Baim, Punti, Chanda, Guchi, Koi, and Taki) were selected for this study. The AAS (Atomic Absorption Spectrophotometer), ICP-MS (Inductively Coupled Plasma-Mass Spectrometry), and the Association of Official Analytical Chemists methods outlined were used to determine the proximate compositional analysis of fish. The highest fat content was found in koi fish. Protein was highest in both baim and taki fish. Iron, zinc, vitamin A, and vitamin B12 content were highest in mola fish. Highest calcium and phosphorus were found in the chanda and taki fish. The study concludes that small indigenous fish are valuable sources of fat, protein, ash, iron, zinc, calcium, phosphorus, potassium, vitamin A, and vitamin B12, which are important for maintaining good health. Our findings suggest that small indigenous fish could serve as a viable option for addressing the nutritional needs for the children (6–23 m age), and Pregnant and lactating women in Bangladesh as a tool to fight malnutrition.