Heavy Metals In Muscle Tissue Research Articles

Comparative Study of Whole-Body Proximate Composition, Dressing Losses and Bioaccumulation of Heavy Metals in Muscle Tissues of Wild and Farm-Raised Labeo Rohita

The muscle tissues of wild and farm-raised rohu were analyzed for proximate composition (Crude protein, crude fat, ash, and dry matter) and uptake of heavy metals (Cd, Ni, Pb, and Cr) using the standard protocols. For this purpose, wild fish samples were collected from the Khanpur Canal, Sheikhupura, and farmed fish samples from a local fish market in Lahore. The results showed that the concentration (%) of protein (17.92±0.36), ash (06.54±0.13), and moisture (76.50±1.53), was observed to be higher in the wild when compared to protein (16.90±0.34), ash (5.07±0.10) and moisture (73.50±1.47) of farm-raised rohu. In contrast, crude fat contents were observed to be higher (4.02±0.08) in farm-raised rohu than wild (03.65±0.07). In terms of dressing losses, wild fish showed higher (26.37%) losses as compared to farm raised (25.83%). Results showed that in wild and farm fish’s muscles, the trend of the heavy metals was Pb > Cd > Ni > Cr. The findings demonstrated that in comparison to Cd, Ni, and Cr, Pb concentration (ppb) was greater in both wild (128.32.82) and farm-raised (111.42.16) rohu. When compared to wild rohu, farmed rohu showed a higher bioaccumulation tendency for Cr and Ni, but Cd and Pb showed the reverse pattern. The findings of this study suggest that fish proximate composition, dressing losses, and bioaccumulation of heavy metals are certainly impacted by the aquatic life of freshwater fish like Labeo rohita both in wild and farmed conditions and these results will be helpful for the aquaculturists as well as the local consumers of the fish.

Relationship of Concentrations of Some Heavy Metals with Fish Size in Muscle Tissue of Carassius gibelio (Bloch, 1782) from the Tigris River (Turkey)

The levels of some heavy metals in muscle tissues (As, Cr, Mn, Co, Ni, Cu and Cd) of Carassius gibelio collected from the Tigris River were analyzed using inductively coupled plasma mass spectrometry (ICP-MS/MS). All the metals in fish muscle were determined. The highest metal concentration in the muscle tissue was found to be Cu and the lowest Co. In regard to heavy metal levels and fish size there were remarkable positive and negative relationships. There was no gender differences in terms of heavy metal accumulation in muscle tissues of Carassius gibelio (p>0.05). The obtained results in this study were lower than the limits for fish which were proposed by WHO, FAO, EPA and TFC.

Influences of Sex and Seasons on Levels of Heavy Metals in Muscle Tissues of Callinectes sapidus Obtained from the Göksu Delta

Influences of Sex and Seasons on Levels of Heavy Metals in Muscle Tissues of Callinectes sapidus Obtained from the Göksu Delta

Composition and human health risk analysis of elements in muscle tissues of wild and farmed fish species from Northeast Mediterranean

It was aimed to determine the concentrations of several heavy metals in muscle tissues of 18 wild and 2 farmed fish species obtained from 6 stations and 3 different fish farms, which were located between Hatay and Antalya provinces in the Northeast Mediterranean. The metal analysis of tissue samples was conducted using the Inductively-coupled Plasma Mass Spectrometry (ICP-MS) method. Given all the tissue samples, the mean metal concentrations were found to be as follows; Li: < 0.001–0.15 mg kg−1 ww, Al: < 0.0006–2.54 mg kg−1 ww, Cr: < 0.001–0.49 mg kg−1 ww, Mn: < 0.0002–1.05 mg kg−1 ww, Fe: < 0.001–0.02 mg kg−1 ww, Cu: < 0.006 – 1.30 mg kg−1 ww, Zn: 1.76–15.4 mg kg−1 ww, As: 0.52–35.1 mg kg−1 ww, Sn: < 0.0004–2.04 mg kg−1 ww. Co, Ni, Cd, and Pb were found to be lower than the detectable limits in all tissue samples. For human consumption estimated weekly intakes (EWI), target hazard quotients (THQ), and cancer risk (CR) levels of the metals were determined in muscle tissues of the wild and farmed fish species. The mean EWI, THQ, and CR levels for As were found 34.82, 3.97E+ 00 ± 4.84E+ 00 and 1.06–03 ± 1.30E-03 for wild species while these values for farmed species were as follows; 2.46, 2.80E-01 ± 1.18E-01 and 7.49E-05 ± 3.15E-05 respectively. When compared to the wild species, metal levels were significantly lower in farmed fishes and consuming these fishes was determined not to cause any health problems. For the wild samples, however, inorganic As concentrations were found to be risky from carcinogenic and non-carcinogenic aspects. Moreover, the mean metal concentrations in muscle tissues of fishes obtained from the Northeast Mediterranean were compared to the national/international codex limits and it was found that all the metal concentrations in tissue samples were lower than the upper limits set by Turkish Food Codex (TFC), European Union (EU), and World Health Organization (WHO).

Hg, As, Cr, Sn, Ni, and Se concentrations in the muscle of little tunny (Euthynnus alletteratus) from the western Algerian stock.

Muscle-metal (As, Se, Hg, Sn, Cr, Ni) levels were measured in little tunny (Euthynnus alletteratus) from the western Algerian stock. Relationships between metal levels in the muscle tissues and fish fork length and total weight were examined. The accumulation of metals in muscle tissues varied in the following ascending order: Ni, Cr, Sn, Hg, Se, and As. The range of metal concentrations (mg kg-1/wet weight) were 1.2561-3.8562 (As), 0.6897-3.4123 (Se), 0.3852-1.4235 (Hg), 0.1569-0.6512 (Sn), 0.1254-0.4002 (Cr), and 0.0719-0.3122 (Ni). Significant variations (t > 1.96, p < 0.05) between metal concentrations were observed in muscle tissues of E. alletteratus. The concentrations of all metals investigated increase as the fish increased in size and total weight. Furthermore, the results of exponential regressions showed significant positive relationships (p < 0.05) between metal levels and fish size and weight. Average levels of heavy metals in muscle tissues of E. alletteratus were lower than permissible limits for fish consumption prescribed by FAO/WHO, EC, ASTDR, and USFDA.

Nutritional value and bioaccumulation of heavy metals in muscle tissues of five commercially important marine fish species from the Red Sea.

The study evaluated the nutritional quality and investigated the heavy metals concentration in muscle tissues of five commercially important marine fish species, including brownspotted grouper (Epinephelus chlorostigma), squaretail coralgrouper (Plectropomus areolatus), black pomfret (Parastromateus niger), goldbanded jobfish (Pristipomoides multidens), and blueskin seabream (Polysteganus coeruleopunctatus) from the Red Sea, Jeddah Coast, Saudi Arabia. Significant differences (p < 0.05) were observed in the proximate chemical composition of fish muscles in these species. The highest protein content (17.66 ± 0.58%) was achieved in blueskin seabream while the lowest (15.28 ± 0.46%) was observed in brownspotted grouper. The highest lipid content (2.97 ± 0.45%) was recorded in squaretail coralgrouper while the lowest (1.52 ± 0.26%) was observed in blueskin seabream. Heavy metal concentrations varied significantly within and between fish species under study (p < 0.05). Significant differences in the concentration of heavy metals among fish species were recorded. Results revealed that the bioaccumulation of Cr, Fe, Ni, and Cd in muscles of fish species under study was higher than the standard concentration, but that of Mn, Cu, and Pb were less than the standard concentration recommended in the EU, FAO, and WHO guidelines. In conclusion, these fish species represent a high-quality food source but is unsafe due to the level of certain minerals in their tissues. Results also indicated that the Red Sea environment is contaminated with heavy metals, which was reflected in the tissues of fishes used in this study.

Heavy metal risk assessment of European eels ( Anguilla anguilla, Linnaeus, 1758) from the Asi (Orontes) River, Turkey

The muscle tissues of a total 154 sampled eels from the Asi (Orontes) River were analyzed between January and December 2018 to determine the content of heavy metals (Hg, Pb, Cd and As). Determination of heavy metals in muscle tissue was carried out with an Inductively Coupled Plasma Mass Spectrometer (ICP-MS). To express the risk level for human consumption of heavy metal contaminated eels we used common concepts and criteria such as Hazard Index (HI), Target Hazard Quotient (THQ) and Total THQ (TTHQ). From the human health point of view, the indices HI, THQ, TTHQ values of Hg, Cd, Pb and As indicate no risks for the consumers at assumed intake levels per unit time.

Bio-accumulation of heavy metals in muscle tissues of a few benthic species in South Gujarat, India

Bio-accumulation of heavy metals in muscle tissues of a few benthic species in South Gujarat, India

Heavy Metals in Muscle Tissue of Pterois volitans from the Veracruz Reef System National Park, Mexico.

Concentrations of cadmium (Cd), lead (Pb), vanadium (V), and zinc (Zn) were measured in the muscle of 30 specimens of Pterois volitans, captured on April 2018, in the Veracruz Reef System National Park (VRSNP), Veracruz, Mexico. Concentrations, in the samples, were quantified with atomic absorption spectrophotometry (AAS), after microwave digestion. Results of the mean concentration, in descending order were V = 7.3 ± 0.7; Pb = 0.66 ± 0.07; Zn = 0.43 ± 0.14; and Cd = 0.03 ± 0.01 mg kg−1 dry weight. These values did not exceeded limits established in the Mexican National Standard (NOM-242-SSA1-2009), of Cd and Pb (0.5 mg kg−1) wet weight. This means that consumption of lionfish from this site does not pose a potential risk for human health.

Measure and Compare Some Heavy Metals in Muscle Tissue of Blue Swimming Crab Portunus pelagicus in Some Areas of the Southern Persian Gulf

Measure and Compare Some Heavy Metals in Muscle Tissue of Blue Swimming Crab Portunus pelagicus in Some Areas of the Southern Persian Gulf

Measure and Compare Some Heavy Metals in Muscle Tissue of Blue Swimming Crab Portunus pelagicus in Some Areas of the Southern Persian Gulf

Measure and Compare Some Heavy Metals in Muscle Tissue of Blue Swimming Crab Portunus pelagicus in Some Areas of the Southern Persian Gulf

Determination of heavy metals in muscle tissue of six fish species with different feeding habits from the Danube River, Belgrade-public health and environmental risk assessment.

The aim of this study was to investigate the presence of heavy metals/metalloids (Pb, Cd, Hg, Cu, Fe, Zn, As) in the muscle tissue of fish from the Danube River (two locations: Zemun and Grocka). For the purpose of heavy metal determination in fish muscle, 120 samples of six different fish species, Prussian carp, barbel, bream, carp, pike perch, and catfish were collected. For determining heavy metals, we used microwave oven digestion and atomic absorption spectrometer methods. The highest average content of Pb (0.084±0.004mgkg-1), Cd (0.082±0.003mgkg-1), Hg (0.466±0.006mgkg-1), and As (0.333±0.007mgkg-1) was found in the muscle of carp (an omnivorous fish) from Grocka, while the highest average level of Fe (13.60±0.03mgkg-1) was deposited in bream (also omnivorous) from Zemun. Also, the average Cu level (1.62±0.13mgkg-1) was the highest in catfish muscle (a carnivorous fish) from Grocka, while the highest Zn content (11.16±0.17mgkg-1) was determined in muscle of Prussian carp (an omnivorous fish) from Zemun. The highest content of heavy metals (Cu, Fe, and Zn, respectively) in muscle of the six different types of fish from both locations was symmetrically arranged by species (catfish, barbel, and Prussian carp, respectively). Concentrations of Pb, Hg, and As in the Danube River fish muscle were under the maximum residual levels prescribed by the European Union (EU) and the maximum allowed concentrations (MAC) for Serbia. On the other hand, in all fish muscle from both locations (Zemun and Grocka), higher concentrations of Cd than prescribed (MAC) were found, with the exception of bream and pike perch.

Heavy metals in muscle tissue of healthy crocodiles from the Kruger National Park, South Africa

Heavy metals in muscle tissue of healthy crocodiles from the Kruger National Park, South Africa

Assessment of some Heavy Metals in Muscle Tissue of Silurus triostegus from Derbendikhan Reservoir, Kurdistan Region –Iraq

Derbendikhan reservoir is located on the south east of Sulaimani province between longitude 35° 6' 35”N and latitude, 45° 41' 20 E, and it is the second largest reservoir in the Iraqi Kurdistan region at an altitude of about 485 m above sea level. More than twenty genera of fishes are endemic to this reservoir, Silurus triostegus is one of them, which was selected to determine the amount of heavy metals that accumulated in the muscle tissue. The results revealed that the metals were accumulated in the following descending order (Fe<Ni <Cu <Cd <Mn) (i.e) Iron have the highest accumulation rate mean of 5.36±16.03 and the Manganese have the lowest rate with the mean of 4.19±.41 and also, the results revealed that the nourishment behavior was played an important role in the accumulation of these elements in the muscle of Silurus triostegus.

Heavy metals in muscle tissues and organs of fish species from Lake Baihua

Concentrations and distribution characteristics of six heavy metals(Pb,Cd,Cu,Cr,As and Hg) in Hypophthalmichthys molitrix and Cyprinus carpio sampled from Baihua Lake were studied by analysis of these heavy metals in their organs(gills,liver,kidney and heart) and muscle tissues(back muscle,abdominal muscle and tail muscle),and their associations with these heavy metals in the water were also examined.The results showed that the average levels of these heavy metals in H.molitrix and C.carpio decreased in the order of Cu Pb Cr Cd As Hg;and Cr and Cu showed a significant correlation in the four organs of H.molitrix and C.carpio with the correlation coefficients of r = 0.878(P 0.01) and r = 0.972(P0.01) respectively,while Cr and Hg in C.carpio presented only a negative correlation with the coefficient of r = 0.782(P0.05).There were significant correlations between concentrations of the heavy metals in the organs of these fish species,but the average levels of these heavy metals in each organ presented low correlations with those in the sediment and overlying water samples.A cluster analysis showed that the six heavy metals in the two fish bodies could be divided into three categories.The content of as in the heart of C.carpio was relatively high,but the content of as in the different organs of H.molitrix had little difference.The level of Hg was relatively uniform in the organs and tissues of the two fish species,the content of these six heavy metals in edible muscle tissue of the fish was generally low.Since heavy metals in sediments from Baihua Lake mainly existed as sulfide compounds that were of low bioavailability,the fish species in this lake were not polluted severely with heavy metals.

Heavy Metals Concentration in Mullet Fish, &lt;i&gt;Liza abu&lt;/i&gt; from Petrochemical Waste Receiving Creeks, Musa Estuary (Persian Gulf)

Fish in estuaries, especially those live near petrochemical area, may be exposed to various kinds of contaminants such as heavy metals. This study was to investigate the variations of heavy metals in Liza abu from the Musa estuary which receives petrochemical wastes. Fish samples were obtained from five different creeks. The samples were dissected into liver, gill and muscle, acid digested and their heavy metals level were analyzed. The results showed that the concentration of Cd, Co, Cu, Ni and Pb in muscle ranged 0.08-0.44, ND-1.63, 0.89 - 4.28, 0.48 - 2.73 and 0.5 - 2.50 (µg/g) respectively. Their concentrations in liver were 0.44 - 2.03, 0.5 - 2.80, 5.05 - 36.22, 0.48 - 4.91 and 0.66 - 5.74 (µg/g) respectively. The concentration of these metals in gill was 0.32 - 2.72, 0.29 - 1.10, 4.33 - 6.03, 4.61 - 17.52 and 2.64 - 21.41 (µg/g) respectively. Generally, the level of heavy metals in muscle tissue of Liza abu was lower than the general standard.

Determination of Heavy Metal Levels in Fish Samples Collected from the Middle Black Sea

Summary The purpose of this study is to determine some heavy metals in muscle tissues of fish collected from the Middle Black Sea Coast of Samsun, Sinop, Terme, Fatsa and Ordu in Turkey. A total of 1650 fish samples including Trachurus trachurus, Alosa caspia, Pomatomus saltatrix, Mullus barbatus, Spicara smaris, Engraulis encrasicolus, Gobius cephalarges, Sarda sarda, Merlangius euxmus and Psetta maxima were used as material. Metal concentrations in fish samples were measured by atomic absorption spectrophotometry. The average value of metal concentrations in fish samples were determined as follows: 2.38 μg/g for Cu, 5.41 for Mn, 26.06 for Fe, 3.40 for Ni, 25.74 for Zn, 0.77 for Pb and 0.022 for Cd, but Hg was not detected. These values were compared with FAO/WHO standards and metal concentrations in fish samples were found to be lower than the maximum permissible levels, but lead level was found to be higher.

Heavy metal concentrations in two barb, Barbus xanthopterus and Barbus rajanorum mystaceus from Atatürk Dam Lake, Turkey

Concentration of some heavy metals (Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb and Zn) were determined in muscle, liver, kidney and gill of two barb, Barbus xanthopterus and Barbus rajanorum mystaceus, which have great economic values, in the Atatürk Dam Lake (Turkey). Heavy metal levels in fish samples were analyzed by inductively coupled plasma spectroscopy (ICP/OES). Heavy metal concentrations vary significantly, depending on the type of the tissue in fish species. The metal accumulation in the liver, kidney and gill of Barbus xanthopterus and Barbus rajanorum mystaceus was found to be quite high in comparison with that in the muscle. The mean concentrations of heavy metals in muscle tissues of Barbus xanthopterus were as follows: Co, 0.09; Cr, 0.12; Cu, 0.27; Fe, 5.26; Mn, 0.20; Ni, 0.08; Pb, 0.68; Zn, 1.39, whereas in muscle tissues of Barbus rajanorum mystaceus were as follows: Co, 0.11; Cr, 0.10; Cu, 1.07; Fe, 3.97; Mn, 019; Ni, 0.04; Pb, 0.66; Zn, 1.70 microg/g wet weight. Cd levels in gill and muscle tissues were below detection limits. All metal levels detected in tissues were safe for human consumption and within the limits for fish proposed by FAO/ WHO, EU and Turkish Food Codes.

Relationship between concentration of heavy metals in muscle tissue and body weight of fish from the Swan-Avon estuary, Westren Austarlia

&lt;P&gt;In five species of teleost from the Swan-Avon river system (&lt;I&gt;Mugil cephalus, Aldrichetta forsteri, Amniataba caudavittatus, Acanthopagrus butcheri&lt;/I&gt; and &lt;I&gt;Nematalosa vlaminghi&lt;/I&gt;), the concentration of nine different heavy metals in muscle tissue either declined or remained relatively constant with increasing body weight. The data for the biologically important iron, zinc, copper and manganese were almost invariably represented better by a logarithmic rather than a linear regression. The concentration of these four metals generally ceased to change markedly with body size at weights above approximately 50 g. The mean concentrations of all nine heavy metals in those individuals heavier than 50 g were very low in every species and are probably close to the minimum levels found in these fish in unpolluted environments. The concentrations of heavy metals were greater in fish which ingested bottom material than in those which fed on organisms such as polychaetes. molluscs and crustaceans, and on various forms of plant.&lt;/P&gt;

Zinc, cadmium, copper, and manganese in species of finfish and shellfish caught in the Derwent Estuary, Tasmania

Thirty-nine marine species, including members of the Teleostorni and Elasmobranchii, were obtained from the River Derwent and analysed for four heavy metals known to be accumulated by certain species, particularly molluscs. Of the muscle tissues from edible Teleostomi and Elasmobranchii all contained levels of zinc, cadmium, and copper well below the maximum levels permitted by current Tasmanian food regulations. Manganese levels were also low. Small species differences for zinc and copper levels in muscle tissue are evident among these finfish. These are probably attributable to differences in feeding habits. Comparison of the metal levels in these species with levels reported by various overseas workers indicate no abnormal accumulation of heavy metals in muscle tissues. With some minor exceptions no relationships existed between the tissue metal levels and length of the fish. Two edible species of molluscs, the native oyster and the common mussel, contained much higher levels of the four metals. For both species levels of zinc and cadmium exceeded the levels specified by the food regulations. The data presented indicate that the ability of molluscs to accumulate certain metals, particularly zinc and cadmium, to abnormally high levels is not shared by finfish species. The fact that elevated metal concentrations in seawater are not reflected in the muscle tissues of finfish means that the monitoring of commercial fish species caught in the River Derwent would provide a much less sensitive index of pollution than would the monitoring of oysters or mussels.