The wood-based composites have received extensive attention in the field of electromagnetic interference (EMI) due to inherent layered porous structure. In this study, the deposition Ni and Cu-Ni on wood surface and heat treatment temperature were used as variables. The variation law of electromagnetic shielding (EMS) performance of composite materials is analyzed. The results showed that Ni particles were fully filled in the layered porous structure of the wood, and the metal coatings evenly covered the entire wood surface. The Ni content of the wood based composite materials via 260 °C heat treatment was up to 98.04 %. The pore structure was more regular with the increase of the number of deposition Ni. The ideal roughness was 11.33 μm and the conductivity reached a maximum of 46.8 S/cm at 200 °C. The EMS effectiveness of one deposition Ni composite materials showed an increasing trend when the heat treatment was 200 ℃, 220 ℃, 240 ℃, 260 ℃ and 280 ℃, respectively. When the heat treatment temperature is 260 °C, the EMS effectiveness of the wood-based composite material can reach 88.76 dB, and the hydrophobicity can reach 99.55 °. When the temperature is 300 °C, the electromagnetic shielding effectiveness can reach 91 dB, and the hydrophobicity can reach 117 °. The anisotropic internal porous structure of wood matrix and the multi-interface polarization between wood and Cu-Ni are the main reasons for the high EMS performance.