Coal mine noise affects human physiology, psychology, and behavior. It causes errors at work and increases accidents. In this study, we built a coal mine noise simulation experiment system. The system not only included an experimental environment simulation system and a physiological indicator test system, but it also added a miners’ working simulation system. This paper aimed to study the effect of different short-term (25 min) noise levels (60 dB, 70 dB, 80 dB, 90 dB, and 100 dB) on human physiology (skin conductivity and heart rate). Critical analysis showed that the stronger the noise intensity is, the shorter the contact time it takes for physiological indicators to present significant changes, and by setting different noises and measuring the skin conductivity and heart rate of human body, it was concluded that the noise level should be reduced to 90 dB to reduce accidents of miners.