Heart Fatty Acid-binding Protein Gene Research Articles

Expression and single-nucleotide polymorphisms of the H-FABP gene in pigs

Heart fatty acid-binding protein (H-FABP) belongs to a family of intracellular fatty acid-binding proteins that are involved in the transport of long-chain fatty acids. Previous studies have indicated that H-FABP is significantly associated with intramuscular fat (IMF) content in pig. In this study, we compared the mRNA and protein expression of H-FABP between Tibetan pig (with high IMF) and Large White pig (with low IMF). The expression of H-FABP at both mRNA and protein levels in the tissues of backfat, longissimus dorsi muscle and liver was found to be significantly higher in TP than in LW. Single-nucleotide polymorphisms (SNPs) in a 2 kb region upstream of the start codon of the gene were screened using Sanger sequencing. We accordingly identified three SNPs (C-1375G, C-314T and T-158G) between the TP and LW populations and genotyped these based on PCR-restriction fragment length polymorphisms (PCF-RFLPs) analysis. The results showed that the C-1375G site might regulate H-FABP gene expression and thus be associated with fat deposition in pigs. Our study provides important data for further investigating the regulatory mechanism of H-FABP for fat deposition in pigs.

Developmental changes in fatty acid-binding protein (H-FABP) mRNA expression and intramuscular fat (IMF) content in Oula sheep.

Increasing meat consumption by Chinese people has created a focus for improving meat quality for increasing quality of life. Twenty-five Male Oula sheep were selected at 2, 21, 56, 84, and 112 d to investigate the developmental changes associated with age on the intramuscular fat (IMF) content of heart fatty acid-binding protein (H-FABP) mRNA expression in muscle. Longissimus dorsal muscle and biceps femoris muscle were sampled to measure IMF concentrations and total mRNA was extracted to measure H-FABP mRNA expression using real-time polymerase chain reaction (PCR). Growing male Oula sheep demonstrated that the IMF concentrations continuously increased with age and significant differences (P < 0.05) were detected among the age groups; 2. The IMF concentrations among tissues were different; 3. The development changes in H-FABP mRNA expression in longissimus dorsal muscle and biceps femoris muscle were similar with a decrease from 2 to 21 d, followed by continuously increasing concentrations being significant different (P < 0.05) among age groups; 4. The H-FABP mRNA expression in the longissimus dorsal muscle tissue was significantly (P < 0.05) higher compared to the biceps femoris muscle; 5. The muscle H-FABP mRNA expression concentration was positively correlated with IMF concentrations from d 21 to 112; 6. The correlation coefficients were significantly (P < 0.01)different between H-FABP gene mRNA expression in the longissimus dorsal muscle and IMF concentration of 0.815 compared to the biceps femoris muscle and IMF concentration of 0.787,which indicated that the H-FABP gene may be affecting the IMF concentrations in the early developmental stages of Oula sheep. These results support the hypothesis that H-FABP gene and its expression in muscle tissue is related to the IMF concentration of meat.

Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition

The aim of the present study was to investigate the associations of single nucleotide polymor - phisms (SNPs) in candidate genes with fatness traits in the Longissimus dorsi muscle of pigs. The polymorphisms of genes were investigated, which included beta-3-adrenergic receptor gene (ADRB3), heart fatty acid-binding protein gene ( FABP3), and hormone-sensitive lipase gene ( LIPE) as well as lipoprotein lipase gene ( LPL). The intramuscular fat (IMF) content and fatty acid composition contents in Longissimus dorsi muscle samples were measured. Results showed that ADRB3, LIPE, and LPL SNPs were associated with IMF content ( P A had highly significant association with the total monounsaturated fatty acid (MUFA) ( P A was significantly associated with the contents of C12:0 and C14:0 ( P T was significantly associated with the percentage of C16:1 ( P < 0.05) and the percentage of total satu - rated fatty acid (SFA) ( P < 0.05). The pigs with ADRB3 G allele had more MUFA, and the pigs with LPL T allele had less SFA, implying that the ADRB3 G and LPL T in pigs may be beneficial to human health. In conclusion, the results suggest that these genetic markers are important sources of the variations for the pork selection to obtain favourable meat with higher IMF levels and appropriate fatty acid composition.

Effects of different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early‐weaned yaks

This study was conducted to estimate different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early-weaned yaks. Results showed that supplementary dietary protein significantly increased final weight, average daily gain (ADG), intramuscular fat (IMF), serum free fatty acid (FFA), total triglycerides, total cholesterol (Ch), low-density lipoprotein cholesterol (LDL) and high-density lipoprotein cholesterol (HDL) content. There was a quadratic response of ADG, IMF, FFA, Ch, HDL and LDL to dietary crude protein (CP) level. Lipoprotein lipase (LPL), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) enzyme activities were significantly increased by supplementary dietary CP, while hormone-sensitive lipase (HSL) and carnitine palmitoyltransferase-1 (CPT-1) activities were significantly decreased. LPL, ACC and FAS enzyme activities showed quadratic increase as dietary CP increased. Peroxisome proliferator-activated receptor γ (PPARγ), LPL, FAS, sterol regulatory element binding protein 1 (SREBP-1), ACC, stearoyl-CoA desaturase (SCD) and heart fatty-acid binding protein (H-FABP) gene expression were significantly increased by supplementary dietary CP, while HSL and CPT-1 gene expression were significantly decreased. PPARγ, LPL, SREBP-1, ACC and H-FABP gene expression showed quadratic increase as dietary CP increased. These results indicated that supplementary dietary protein increased IMF accumulation mainly to increased intramuscular lipogenic gene expression and decreased lipolytic gene expression.

Investigation of the association of two candidate genes (H-FABP and PSMC1) with growth and carcass traits in Qinchuan beef cattle from China

Growth and carcass traits are economically important quality characteristics of beef cattle and are complex quantitative traits that are controlled by multiple genes. In this study, 2 candidate genes, H-FABP (encoding the heart fatty acid-binding protein) and PSMC1 (encoding the proteasome 26S subunit of ATPase 1) were investigated in Qinchuan beef cattle of China. PCR-SSCP and DNA sequencing methods were used to detect mutations in the H-FABP and PSMC1 genes in Qinchuan cattle, and a T>C mutation in exon 1 of H-FABP and a T>C mutation in exon 9 of PSMC1 were identified. The association of these 2 single nucleotide polymorphisms with growth and carcass traits of Qinchuan cattle was analyzed. The T>C mutation in H-FABP was significantly associated with body length and dressing percentage (P < 0.05) and the T>C mutation in PSMC1 with body length and hip width (P < 0.05), indicating that both of the 2 mutations in H-FABP and PSMC1 had effects on growth and carcass traits in the Qinchuan beef cattle breed. Thus, the results of our study suggest that the H-FABP and PSMC1 gene polymorphisms could be used as genetic markers in marker-assisted selection for improving Qinchuan beef cattle.

Cloning of the heart fatty acid-binding protein (H-FABP) gene and its tissue-specific expression profile in the Lanzhou fat-tailed sheep, Ovis aries

The heart fatty acid-binding protein gene (h-fabp) plays an important role in intracellular fatty acid transport. h-fabp had been cloned and sequenced and is considered a functional candidate gene for assessing the meat quality traits in pig, chicken, and cattle. However, there have only been few studies reported on this gene in sheep. In the present study, we cloned the full-length cDNA of h-fabp in the Lanzhou fat-tailed sheep using RACE technique. Its tissue specific expression profile in the skeletal muscle, liver and 4 fat depots at seven developmental stages was determined by real-time quantitative PCR. A 425bp 5′-RACE cDNA, 231bp 3′-RACE cDNA and 177bp intermediate fragment were spliced to obtain a 748bp full-length cDNA of h-fabp (GenBank accession number JQ780322). The phylogenetic analysis revealed that the Lanzhou fat-tailed sheep is more closely related to the goat, Capra hircus. In skeletal muscle, h-fabp was expressed at a very low level in one-month-old sheep. The expression level increased in three- and five-month-old sheep, and a peak expression level was observed in seven-month-old sheep. In nine- and eleven-month-old sheep, the gene expression level was similar to the mRNA level observed in three- and five-month-old sheep. In thirteen-month-old sheep, the h-fabp mRNA level was similar to the low expression level observed in one-month-old sheep. The expression pattern observed in the skeletal muscle at different developmental stages in sheep indicates that h-fabp expression declines with age. A similar conclusion on the h-fabp expression pattern in the liver was drawn from our findings in this study. The amount of h-fabp mRNA in the subcutaneous (s.c.) and visceral (v.s.) depots at each developmental stage was very low, and there was no significant difference in the expression levels among these depots. When we added the data from the seven developmental stages together, however, the highest h-fabp expression level was observed in the s.c. fat near the wither (s.c.W), followed by the v.c. perirenal (v.c.P), v.c. omental (v.c.O), and s.c. fat near the base of tail (s.c.T). These findings provide basic data that may be useful in future studies that investigate the role h-fabp plays in fat deposition and the regulation of fatty acid metabolism in sheep.

Effect Analysis of Heart Fatty Acid-Binding Protein Gene Assisted Selection on Intramuscular Fat Content in Chicken

Effect Analysis of Heart Fatty Acid-Binding Protein Gene Assisted Selection on Intramuscular Fat Content in Chicken

Polymorphisms of candidate genes associated with meat quality and disease resistance in indigenous and exotic pig breeds of Vietnam

The objectives of this study were to analyse genotype distribution and sequence variations of candidate genes putatively associated with meat quality and disease resistance in exotic and indigenous Vietnamese pig breeds. For this purpose, 340 pigs from four indigenous and two exotic breeds were included in the analysis of the polymorphisms of the heart fatty-acid-binding protein (H-FABP), alpha 1 fucosyltransferase (FUT1), and bactericidal/permeability-increasing protein (BPI) genes by the sequencing and PCR-RFLP methods. For H-FABP, 17 single nucleotide polymorphisms (SNPs) were detected in indigenous pig breeds by direct sequencing of a fragment at intron 2 of the H-FABP gene. The mutation T1556C created a new restriction site for the enzyme MspI, which gave rise to new allelic variants in three indigenous pig breeds. In indigenous breeds, the frequency of the favourable alleles a and d at MspI and HaeIII sites of the H-FABP gene were low. Meanwhile, the frequency of the d allele at the HaeIII site in exotic breeds was significantly higher than those of indigenous pig breeds. No mutation was found in the RFLP-fragment of the FUT1 gene of four indigenous pig breeds by sequencing, while in the BPI gene two mutations were detected in the Tap Na breed. The resistant alleles of the FUT1 and BPI genes in the exotic breeds were significantly higher than those of indigenous pig breeds. Among the indigenous pig breeds, the Tap Na breed possessed a higher frequency of the resistant allele G of BPI gene than the remaining breeds. The T1556C mutation at H-FABP may be important for the genetic improvement of intramuscular fat content and breed. Tap Na may be a source of resistant alleles for local ecologies.

Investigation of four porcine candidate genes (H-FABP, MYOD1, UCP3 and MASTR) for meat quality traits in Large White pigs

Meat quality traits are economically important traits of swine, and are controlled by multiple genes as complex quantitative traits. In the present study four genes, H-FABP (heart fatty acid-binding protein), MASTR (MEF2 activating motif and SAP domain containing transcriptional regulator), UCP3 (uncoupling protein 3) and MYOD1 (myogenic differentiation 1) were researched in Large White pigs. The polymorphisms H-FABP T/C of 5'UTR, MYOD1 g.257 A>C, UCP3 g.1406 G>A in exon 3 and MASTR c.187 C>T have been reported to be associated with meat quality traits in pigs. The aim of this study was to analyze the effect of single and multiple markers for single traits in Large White pigs. The single marker association analysis showed that the H-FABP and MASTR genes were associated with IMF (intramuscular fat content) (P < 0.05), and that the g.257 A>C of MYOD1 gene was most significantly related to muscle pH value (P < 0.01). The multiple markers for IMF were analyzed by combining the markers and quantitative trait modes into the linear regression. The results revealed that H-FABP and MASTR integrate gene networks for IMF. Thus, our study results suggested that H-FABP and MASTR polymorphisms could be used as genetic markers in the marker-assisted selection towards the improvement of IMF in Large White pigs.

Effects of dietary protein/carbohydrate ratio on fat deposition and gene expression of peroxisome proliferator activated receptor γ and heart fatty acid-binding protein of finishing pigs

The present study was conducted to investigate the effect of dietary protein/carbohydrate (CH2O) ratio on fat deposition and expression of peroxisome proliferator activated receptor γ (PPARγ) and heart fatty acid-binding protein (H-FABP) genes in muscle and subcutaneous adipose tissue in pigs. Twenty pigs (73.9±1.2kg BW) were used in a single factorial experiment, and allocated by BW and ultrasound backfat thickness to the two treatments: low protein/CH2O ratio (LP; 11.2% CP and 68.2% CH2O for phase I, and 10.1% CP and 69.3% CH2O for phase II) diet, or high protein/CH2O ratio (HP; 22.7% CP and 58.3% CH2O) diet. Pigs were housed individually in pens, and had ad libitum access to feed and water. After 80-d feeding, 6 pigs from each treatment were selected and slaughtered. Dietary protein/CH2O ratio had no effect on growth performance; however, the intramuscular fat (IMF) in longissimus muscle (LM) was increased (P<0.01) when the LP diet was fed. Correspondingly, Warner–Bratzler sheer force of LM in the LP pigs was lower (P<0.05) than the HP pigs. The LP increased mRNA levels of PPARγ (P<0.05) and H-FABP (P=0.09) in LM but not in subcutaneous fat, although the difference of H-FABP gene expression in LM was not statistically significant. The mRNA abundance of PPARγ in muscle correlated positively with IMF content (P<0.05). The results indicated that an increase in IMF but not subcutaneous fat in pigs fed the LP diet is related to tissue-specific activation of PPARγ and H-FABP mRNA expression, especially the PPARγ gene.

Polymorphisms of MRF4 and H-FABP genes association with growth traits in Qinchuan cattle and related hybrids

PCR-RFLP was applied to analyse polymorphisms within the MRF4 and heart fatty acid-binding protein (H-FABP) gene for correlation studies with growth traits in three-month-old Qinchuan (QQ), Qinchuan × Limousin (LQ) and Qinchuan × Red Angus (AQ) cattle. The results showed that 874 bp PCR products of MRF4 digested with XbaI and 2,075 bp PCR products of H-FABP digested with HaeIII were polymorphic in the three populations. Moreover, the frequencies of allele A at MRF4 locus and allele B at H-FABP locus in the QQ, AQ, and LQ populations were 0.8358/0.8888/0.8273 and 0.8358/0.7500/0.8195 respectively. Allele A at MRF4 locus and allele B at H-FABP locus were dominant in the three populations. No statistically significant differences in growth traits were observed among the genotypes of the all three populations at H-FABP locus. However, the association of MRF4 polymorphism with growth traits was then determined in all three populations. The body weight, withers height, heart girth and height at hip cross of individuals with genotype AA were higher than those with genotype AB or BB (P < 0.05). Therefore, we suggest that the MRF4 gene may function in the control or expression of growth traits, particularly body weight, withers height, heart girth and height at hip cross.

Gene Expression of Heart and Adipocyte Fatty Acid-binding Protein in Chickens by FQ-RT-PCR

This study was to detect the expression of heart fatty acid-binding protein (H-FABP) and adipocyte fatty acid-binding protein (A-FABP) gene mRNA in different tissues of Rugao and Luyuan chickens at 56 d and 120 d by real-time fluorescence quantitative reverse transcription polymerase-chain reaction (FQ-RT-PCR). The primers were designed according to the sequences of H-FABP, A-FABP and GAPDH genes in Gallus gallus, which were used as target genes and internal reference gene, respectively. The levels of H-FABP and A-FABP gene expression were detected by SYBR Green I FQ-RT-PCR. The relative H-FABP and A-FABP gene mRNA expression level was calculated with 2 -ΔCt . Melting curve analysis showed a single peak of three genes. Intramuscular fat (IMF) content in breast muscle and leg muscle of the two chicken breeds at 120 d was higher than at 56 d. IMF content in breast muscle and leg muscle at 56 d and 120 d in Luyuan was significantly higher than in Rugao, however, abdominal fat of Luyuan was significantly lower than that of Rugao. The relative H-FABP gene mRNA expression level in cardiac muscle was the highest in both chicken breeds. The relative H-FABP and A-FABP gene expression of different tissues in Luyuan was higher than in Rugao. H-FABP gene mRNA expression had a negative effect on IMF of leg and breast muscles, and was significantly negatively correlated with IMF content. The relative A-FABP gene mRNA level in abdominal fat was higher than in liver. The A-FABP gene mRNA was not expressed in leg, breast and cardiac muscles. A-FABP gene mRNA expression level was significantly positively correlated with abdominal fat and had a significant effect on abdominal fat but not IMF content.

Study on single nucleotide polymorphisms of &lt;I&gt;H –FABP&lt;/I&gt; gene in sheep

PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48), Ningxia Tan sheep (Tan, 121), Tan x SH F1 (23), Poll Dorset (48) and Suffolk (24) sheep were screened in this study. The result showed: (1)four SNPs at 981(G/A), 1014(A/C) 1019(T/C) and 1058 (-/G ) and nine genotypes (AA, BB, CC, AB, AC, BC, AD, CD and BD) were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content (PIC) in the Suffolk and Tan x SH F1 populations (PIC amp; 0.25) but an intermediate PIC in the remaining three populations (0.25 amp; PIC amp;0.50). It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. (2)Three genotypes (HH, Hh and hh) determined by a SNP at 2407(T-C) were detected using primer 4. The genotype frequencies were in the order of HHHhhh. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low (PIC amp; 0.25). However, there was no polymorphisms in SH, Tan x SH F1 and Suffolk populations.

Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs

The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P&lt;0.05), jowl (P&lt;0.05), sirloin (P&lt;0.10), and kidneys (P&lt;0.01). These results showed the potential of the H-FABP gene in marker-assisted selection programs for carcass traits in pigs.

Cloning and sequencing of GH, H-FABP and HSP72 genes in yak

The growth hormone (GH) gene, the heart fatty acid binding protein (H-FABP) gene and the heat shock protein 72 (HSP72) gene of the yak (Bos grunniens) were cloned and sequenced. The sequences were compared with those of other animals, and phylogenetic trees were constructed by the NJ (neighborhood joining) method. Results showed that the yak GH gene was composed of five exons (13, 161, 117, 162 and 198 bp) and four introns (248, 225, 229 and 275 bp). The cDNA of GH was a 654 bp nucleotide encoding a putative protein of 217 amino acid (AA) residuals, with a signal peptide of 26 AAs and the mature peptide of 191 AAs. The H-FABP gene was composed of four exons (73, 173, 102 and 54 bp) and three introns (3460, 1892 and 1495 bp). The cDNA was 402 bp encoding a putative protein of 133 AAs. The yak HSP72 gene was an intron-free 1926bp nucleotide, encoding a protein of 641 AAs. The data suggest that the three genes from the yak were highly conserved with other species at the nucleic acid level. The results of the phylogenetic trees reflect the molecular evolution relationship among these species, consistent with the zoological classification. The genetic distance calculated by the nucleotide sequence of the GH gene’s coding region indicated that genetic distances among yak, cattle, gayal and zebu were relatively small, but genetic distances between these four species and buffalo were relatively large. Therefore, it was more reasonable to consider yak, cattle, gayal and zebu as independent species of the genus Bos, while buffalo belongs to another category (Bubalus). Key words: Yak, GH gene, H-FABP gene, HSP72 gene, phylogenetic evolution

Identification of Single Nucleotide Polymorphism of H-FABP Gene and Its Association with Fatness Traits in Chickens

: Heart fatty acid-binding protein gene (H-FABP) is an important candidate gene for meat quality. One of the objectives of this study was to screen single nucleotide polymorphisms (SNP) of chicken H-FABP gene among 252 individuals that included 4 Chinese domestic chicken breeds (Fengkai Xinghua (T04), Huiyang Huxu (H), Qingyuan Ma (Q), Guangxi Xiayan (Sl)), 2 breeds developed by the Institute of Animal Science, Guangdong Academy of Agricultural Sciences (Lingnan Huang (DC), dwarf chicken (E4)) and one introduced broiler (Abor Acre (AA)). Another objective of this study was to analyze the associations between polymorphisms of the H-FABP gene and fat deposition traits in chickens. PCR-SSCP was used to analyze SNPs in H-FABP and 4 SNPs (T260C, G675A, C783T and G2778A) were detected. Associations between polymorphic loci and intramuscular fat (IMF), abdominal fat weight (AFW) and abdominal fat percentage (AFP) were analyzed by ANCOVA method. The results showed that the T260C genotypes were significantly associated with IMF (p = 0.0233) and AFP (p = 0.0001); the G675A genotypes were significantly associated with AFW, AFP (p<0.01) and IMF (p<0.05); at the C783T locus, AFW and AFP differed highly between genotypes. However, the G2778A loci did not show any significant effect on fat deposition traits in this study. In addition, we found that there were some differences between AFP and definite haplotypes through a nonparametric statistical method, so the haplotypes based on the SNPs except G2778A loci were also significantly associated with IMF, AFW (g) (p<0.05) and AFP (%) (p<0.001). Significantly and suggestively dominant effects of H4H4 haplotype were observed for IMF and the H2H3 was dominant for AFW (g) and AFP (%). The results also revealed that H5H7 haplotype had a negative effect on IMF, while the H5H6 had a positive effect on AFW (g) and AFP (%).

Polymorphism of Heart Fatty Acid-Binding Protein Gene Associatied with Fatness Traits in the Chicken

Fatty acid-binding proteins (FABP) belong to a superfamily of lipid binding proteins that exhibit a high affinity for long chain fatty acids and appear to function in metabolism and intracellular transportation of lipids. The current study was designed to investigate the effects of heart (H)-FABP gene on chicken growth and body composition traits. The Northeast Agricultural University divergent broiler lines for abdominal fat and a broiler X silkie F2 population were used in this study. Body weight and body composition traits were measured in the populations. Primers were designed according to the chicken H-FABP gene sequence. Polymorphisms between parental lines were detected by DNA sequencing. PCR-RFLP and PCR-fragment length polymorphism methods were developed to genotype the populations. The results showed that the H-FABP gene polymorphisms in the two populations were associated with abdominal fat percentage. It implied that H-FABP gene can be a candidate locus or linked to a major gene(s) that affects abdominal fat content in the chicken.

Cell cultures harbouring constructs of different pig promoter polymorphisms show different transcriptional efficiency in gene reporter systems

Production traits variability among and within breeds, differences among developmental stages or the response to different environments are in part due to genetic factors that affect gene expression. Within the context of an Italian FIRB project, whose objective is to identify genes and molecular mechanisms affecting meat quality and production traits in pig, we studied the promoter regions of candidate genes selected on the basis of their physiological role in animal tissue development or composition. Genomic DNA was isolated from liver or muscle tissue of individuals belonging to Large White and Casertana breed. PCR primers were designed to amplify 5’ upstream region of SCD (Stearoyl-CoA Desaturase), LDLR (Low Density Lipoprotein Receptor), LEP (Leptin), MSTN (Myostatin), ACTA1 (Alpha-actin) and HFABP (Heart Fatty Acid Binding Protein) genes using sequences available at NCBI. A total of 19 single nucleotide polymorphisms (SNPs) not previously described were characterised. Some haplotypes, harbouring SNPs located within, or closely flanking potential cis-acting elements, were used to carry out an in vitro analysis of the efficiency of promoter natural variants. Up to 1200 bases upstream the translation start codon were cloned in pGL3 basic vector in phase with the downstream luciferase reporter gene. The different luminescence intensities showed by constructs harbouring allelic variants suggest transcriptional efficiency influenced by polymorphism. The knowledge of a correlation between a different transcriptional activity and a particular haplotype could be a useful tool to identify variation at genes controlling important traits in pig.

Polymorphism of the H-FABP, MC4R and ADD1 genes in the Meishan and four other pig populations in China

Genes such as the Heart Fatty Acid-Binding Protein (H-FABP), the Melanocortin-4 Receptor (MC4R) and the Adipocyte Determination and Differentiation factor-1 (ADD1) play an important role in meat quality in pigs. The Meishan is one of the most prolific pig breeds in the world, but it is in danger, in China, of being replaced by other exotic pig breeds because of its slow growth rate and high body fat content. To know their present genetic status, the polymorphism of the three genes in the Meishan and four other pig populations (Sutai, Yorkshire×Sutai, Landrace×Sutai and Duroc×Landrace×Yorkshire) in China was analysed, using PCR-RFLP and PCR-SSCP, and were further compared with each other. The results showed that gene and genotype frequencies differed significantly between the Meishan and the other four populations. This is suggested to be one of the reasons for the good meat quality of the Meishan compared to the other breeds. In addition, since half of the genetic background of Sutai is from the Meishan, results also indicated that marker assisted selection (MAS) is very important for the development of new pig breeds. South African Journal of Animal Science Vol. 36(1) 2006: 1-6

Relationship Among H-FABP Gene Polymorphism, Intramuscular Fat Content, and Adipocyte Lipid Droplet Content in Main Pig Breeds with Different Genotypes in Western China

H-FABP (Heart fatty acid-binding protein), a member of FABP family, plays an essential role in long-chain fatty acid uptake and metabolic homeostasis. Its role in pig intramuscular fat content remains poorly understood, especially in local pig breeds in western China. In this study, the genetic variations of 5′-upstream region and the second intron in porcine H-FABP gene were investigated by PCR-RFLP in 256 pigs including Duroc, Large White, Landrace, Neijiang, Rongchang, Bamei pig, Hanjiang Black, Hanzhong White, and the wild ones. The effect of H-FABP gene on the IMF content was analyzed by the least square method. Lipid droplet morphology and content in adipocytes cultured from pigs with different H-FABP genotypes, were studied by oil red O staining and a triglyceride assay kit. Results showed a Hinf I -RFLP in these eight pig breeds and wild pigs, among which Large white, Bamei pig, Hanjiang Black, Hanzhong White, and wild pigs presented with low polymorphism while the other breeds had intermediate polymorphism. There was no Hae III or Msp I -RFLPs in the four Chinese local pig breeds tested, but Duroc, Landrace, Large White, Hanzhong White and wild pig had polymorphism. Landrace, Large White and wild pigs had low levels of Hae III-and Msp I -RFLP, whereas others had intermediate polymorphism. H-FABP genotypes significantly affected the IMF content ( P<0.05). The IMF content ordered by H-FABP genotypes were HH> Hh> hh, DD< Dd< dd, and AA< Aa< aa. The genetic effect values were 3.89, 3.42, 3.17, 2.27, 2.49, 2.91, 2.28, 2.70, and 2.95, respectively. Fat deposition in adipocytes was stronger in the HH, dd and aa genotypes than in others. The results suggest that porcine meat quality may be improved by increasing the frequency of genotype aa- dd- HH in pig breeds.