Healthy Farm Research Articles

Evaluation of methods for detection of Campylobacter in raw milk: A multi-country study

Raw milk is considered a high-risk source of Campylobacter due to faecal contamination from healthy cattle and farm environments, thus linking raw milk consumption to global outbreaks. Detection of Campylobacter in raw milk poses challenges due to low contamination levels and antibacterial properties of the milk. Culture-based protocols for Campylobacter detection in milk vary, mainly with regard to pH adjustment and the choice of enrichment broth.This European collaborative study was organised by the EU Reference Laboratory (EURL) for Campylobacter together with eight EU National Reference Laboratories (NRL) for Campylobacter with the purpose to evaluate methods for culture-based detection of Campylobacter in raw cow's milk. The study was divided into two parts, an interlaboratory part and an intralaboratory part, both organised around the same two protocols. The aim of protocol 1 was to evaluate the impact of pH adjustment and storage of the milk on the culturability of Campylobacter over time. Aliquots of the spiked milk were adjusted either to pH 7.0 or pH 7.6 or left unadjusted. The milk was stored up to 48 h at refrigerated temperature and Campylobacter was quantified according to ISO 10272-2 on day 0, 1 and 2. The aim of protocol 2 was to evaluate which enrichment broth, Bolton broth (BB) or Preston broth (PB), showed highest sensitivity in detection of Campylobacter. The spiked milk was enriched in BB and PB as described in ISO 10272-1:2017 or ISO 10272-1:2017/Amd1.2023. In the interlaboratory part, each milk batch was collected locally by each participating NRL/EURL and inoculated with the same Campylobacter strain. In the follow-up intralaboratory part, the EURL-Campylobacter repeated the tests in protocol 1 and 2 but used different Campylobacter strains and strains subjected to thermal stress prior to inoculation.The results show that pH adjustment of raw milk has a negligible impact on culture-based detection of Campylobacter, regardless of strain and level of environmental stress. The composition of milk and properties of the inoculated strain influence culture-based detection of Campylobacter over storage time, and strains subjected to additional stress prior to inoculation in milk are reduced in culturability much faster than the same strains prepared under normal conditions.Finally, the study showed that PB without Campylobacter growth supplement is less effective than BB in detecting Campylobacter in raw milk.

Escherichia coli from healthy farm animals: Antimicrobial resistance, resistance genes andmobile genetic elements.

The use of antibiotics in agriculture and subsequent environmental pollution are associated with the emergence and spread of multidrug-resistant (MDR) bacteria including Escherichia coli. The aim of this study was to detect antimicrobial resistance, resistance genes and mobile genetic elements of 72 E. coli strains isolated from faeces of healthy farm animals. Disk diffusion test showed resistance to ampicillin (59.7%), tetracycline (48.6%), chloramphenicol (16.7%), cefoperazone and ceftriaxone (13.9%), cefepime and aztreonam (12.5%), norfloxacin and ciprofloxacin (8.3%), levofloxacin (6.9%), gentamicin and amikacin (2.8%) among the studied strains. Antibiotic resistance genes (ARGs) were detected by polymerase chain reaction: the prevalence of blaTEM was the highest (59.7% of all strains), followed by tetA (30.6%), blaCTX-M (11.1%), catA1 (9.7%), less than 5% strains contained blaSHV, cmlA, floR, qnrB, qnrS, tetM. 26.4% of E. coli strains had a MDR phenotype. MDR E. coli more often contained class 1 integrons, bacteriophages, conjugative F-like plasmids, than non-MDR strains. ARGs were successfully transferred from faecalE. colistrains into theE. coliNissle 1917 N4i strain by conjugation. Conjugation frequencies varied from (1.0 ± 0.1) * 10-5 to (7.9 ± 2.6) * 10-4 per recipient. Monitoring mobile genetic elements of E. coli for antibiotic resistance is important for farm animal health, as well as for public health and food safety.

Ecological processes of bacterial microbiome assembly in healthy and dysbiotic strawberry farms

The bacterial microbiome plays crucial role in plants’ resistance to diseases, nutrient uptake and productivity. We examined the microbiome characteristics of healthy and unhealthy strawberry farms, focusing on soil (bulk soil, rhizosphere soil) and plant (roots and shoots). The relative abundance of most abundant taxa were correlated with the chemical soil properties and shoot niche revealed the least amount of significant correlations between the two. While alpha and beta diversities did not show differences between health groups, we identified a number of core taxa (16–59) and marker bacterial taxa for each healthy (Unclassified Tepidisphaerales, Ohtaekwangia, Hydrocarboniphaga) and dysbiotic (Udaeobacter, Solibacter, Unclassified Chitinophagales, Unclassified Nitrosomonadaceae, Nitrospira, Nocardioides, Tardiphaga, Skermanella, Pseudomonas, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Curtobacterium) niche. We also revealed selective pressure of strawberry rhizosphere soil and roots plants in unhealthy plantations increased stochastic ecological processes of bacterial microbiome assembly in shoots. Our findings contribute to understanding sustainable agriculture and plant-microbiome interactions.

Novel in-genome based analysis demonstrates the evolution of OmpK37, antimicrobial resistance gene from a potentially pathogenic pandrug resistant Klebsiella pneumoniae MS1 isolated from healthy broiler feces

Antimicrobial resistance transmission from farm animals to humans is a critical health concern and hence a detailed molecular surveillance is essential for tracking the spread and consequent evolution of antimicrobial resistance. In this study, a pan-drug resistant Klebsiella pneumoniae MS1 strain was isolated from a healthy broiler farm and studied. From the results of the study, MS1 was found to be is resistant to 18 tested antibiotics and has a high-risk to be pathogenic to humans with a probability of 0.80. The whole genome sequencing data of MS1 was used to predict the presence of antimicrobial resistance genes and pathogenicity. The genome analysis has revealed MS1 to have 34 AMR genes. Out of these, the AMR gene OmpK37 codes for an important protein involved in cell permeability and hence in antibiotic resistance. Further analysis was carried out by using an in-genome analysis method to understand the evolution of OmpK37 and the underlying reason for the emergence of resistance. From the detailed analysis, the current study could demonstrate for the first time the evolution of OmpK37 from OmpC. Though structurally OmpK37 was very similar to other porins present in MS1, it was found to have higher mutability as a distinguishing feature which makes it an important protein in monitoring the evolving resistances in microorganisms.

Prevention of Ancylostomiasis Transmission in Livestock and Stockmen with Good Hygiene Behavior and Cage Sanitation

Introduction: Livestock farming is a vulnerable area for the transmission of zoonotic diseases. Good cage management affects the health of livestock and workers. Furthermore, clean and healthy farm conditions help to maintain the health of the people living around the farm. This study was centered on the early detection of worm infections in livestock. Methods: This exploratory study was conducted on a village farm containing 52 cows with 20 workers. Helminthiasis screening was performed with the saturated NaCl flotation technique via laboratory testing. Results: It was found that one cow was infected with hookworm, and all the workers were in healthy condition. Conclusion: Transmission of ancylostomiasis can be prevented by maintaining cage sanitation and worker hygiene behavior.

Strategies for reducing noxious gas emissions in pig production: a comprehensive review on the role of feed additives.

The emission of noxious gases is a significant problem in pig production, as it can lead to poor production, welfare concerns, and environmental pollution. The noxious gases are the gasses emitted from the pig manure that contribute to air pollution. The increased concentration of various harmful gasses can pose health risks to both animals and humans. The major gases produced in the pig farm include methane, hydrogen sulfide, carbon dioxide, ammonia, sulfur dioxide and volatile fatty acids, which are mainly derived from the fermentation of undigested or poorly digested nutrients. Nowadays research has focused on more holistic approaches to obtain a healthy farm environment that helps animal production. The use of probiotics, prebiotics, dietary enzymes, and medicinal plants in animal diets has been explored as a means of reducing harmful gas emissions. This review paper focuses on the harmful gas emissions from pig farm, the mechanisms of gas production, and strategies for reducing these emissions. Additionally, various methods for reducing gas in pigs, including probiotic interventions; prebiotic interventions, dietary enzymes supplementation, and use of medicinal plants and organic acids are discussed. Overall, this paper provides a comprehensive review of the current state of knowledge on reducing noxious gas in pigs and offers valuable insights for pig producers, nutritionists, and researchers working in this area.

Metabolic and Hematological Biomarkers Alterations during the Transition Period in Healthy Farm Animals: A Review

Metabolic and Hematological Biomarkers Alterations during the Transition Period in Healthy Farm Animals: A Review

English

The aim of this study is to understand molecular characterization and antimicrobial resistance of diarrheagenic and nondiarrheagenic E. coli from healthy and diseased farm fish from Cypriniformes order. A total of 516 healthy and diseased farmed fish samples belong to Cypriniformes order were collected from various regions of Punjab and screened for E. coli. Biochemical tests and PCR confirmation were conducted using the 16S rRNA and UspA genes. DEC was examined through PCR using different virulence genes. All isolates were tested for various antibiotics using the disk diffusion method. The phenotypic and genotypic resistances of Extended-spectrum β-lactamases (ESBLs) were investigated. A Chi-square test was performed to compare the prevalence of E. coli, virulence genes, and ESBLs genes with a significance level of P ≤ 0.05. Out of the total fish samples, 16.66% and 36.57% of E. coli strains were confirmed through biochemical tests and PCR, respectively. The DEC pathotypes were recorded in 30% and 62.02% of isolates from healthy and diseased fish, respectively. A higher rate of 19.37% of EAEC pathogroup was observed in both healthy and diseased fish, while the EIEC pathogroup was only observed in diseased fish at a rate of 10.07%. The significance (P ≤ 0.05) was observed among the virulence genes. The highest resistance rates among diarrheagenic and non-diarrheagenic strains were observed with tetracycline, amoxicillin, and ceftazidime. A higher prevalence of CTX-M (56.81%) and TEM (43.18%) was recorded in DEC pathotypes, while blaSHV was not detected. The high prevalence of DEC E. coli, and the production of ESBLs, indicate unhygienic practices and the misuse of antibiotics in fish farms.

Severely Asthmatic Horses Residing in a Mediterranean Climate Shed a Significantly Lower Number of Parasite Eggs Compared to Healthy Farm Mates.

The relationship between helminth infection and allergic diseases has long intrigued the scientific community. This interaction was previously studied in a horse family with high incidence of severe equine asthma and in non-related severely asthmatic horses from equine hospital referrals in Switzerland. Our aim was to determine if this interaction would also be observed in a group of non-related client-owned severely asthmatic horses living in a Mediterranean climate and recruited through a first-opinion veterinarian group. Fecal samples from severe equine asthma-affected and healthy horses living in the same farms and subjected to identical environmental and deworming management were evaluated qualitatively and quantitatively. Strongyle-type eggs and Cyathostomum sensu latum larvae were the most abundant parasites in the studied population of horses; no significant differences between the groups were observed regarding the types of egg and infective larvae. However, we observed significant differences in the number of eggs and infective larvae per gram of feces shed, as this number was significantly lower in the SEA group than in the healthy horses. This may indicate that severely asthmatic horses have an intrinsic resistance to gastrointestinal helminths. Further studies in a larger population of horses are required to ascertain the immunological mechanisms responsible for these findings.

Quantitative analysis of rhizosphere microbial population obtained from main-croplands in South Sulawesi

The study aims to investigate microbial species and quantify population richness associated with main crop rhizosphere in South Sulawesi. Survey was conducted in two areas including healthy and infected croplands. In each area about 1 ha, samples were collected to consist of 250 g bulk soil of rhizosphere in healthy and infected farms 1 month after transplanting. For analysis, those samples were carried out at the Plant pathology Lab, Department Plant Pest and Disease, Faculty of Agriculture. 10 g bulk soil separation out of 250 g obtained from rhizosphere was sterilized into water 25 mL and sieved 50-100 mesh prior to transfer to growth medium. After that, 4 % (g/mL) potato dextrose agar (PDA) in the petri dish was earlier prepared. The soil mixture (1g/10 mL) was diluted and transferred into a series dilution and parallelly loaded into solid media kept in dark room with at room temperature. Microbiota successfully growing in the solid media were split and transferred into petridish contained PDA for morphological identification and quantified through a series dilution. The study found 23 native fungal isolates consisting of Genera: Rhizopus sp. Phytophthora sp., Fusarium sp., Rhizoctonia sp., Geotrichum, Aspergillus sp., Gliocladium sp., Gongronella sp., and 14 unknown groups of fungi. Three bacterial isolates were discovered as gram-positive bacteria (GPB) in the infected cropland and the other two were gram-negative bacteria (GNB) found in healthy farm. The finding evidenced that population of microbiota ‘bacterial and fungal’ richness was twofold much higher in the healthy farm than infected farm. Bacterial population was the highest in both healthy and infected farms. There is a tendency of the higher fungal population richness, the lower rice disease incidence in croplands, vice versa.

Species Profiles and Antimicrobial Resistance of Non-aureus Staphylococci Isolated from Healthy Broilers, Farm Environments, and Farm Workers.

Non-aureus staphylococci (NAS), particularly antimicrobial-resistant NAS, have a substantial impact on human and animal health. In the current study, we investigated (1) the species profiles of NAS isolates collected from healthy broilers, farm environments, and farm workers in Korea, (2) the occurrence of antimicrobial-resistant NAS isolates, especially methicillin resistance, and (3) the genetic factors involved in the methicillin and fluoroquinolone resistance. In total, 216 NAS isolates of 16 different species were collected from healthy broilers (n=178), broiler farm environments (n=18), and farm workers (n=20) of 20 different broiler farms. The two most dominant broiler-associated NAS species were Staphylococcus agnetis (23.6%) and Staphylococcus xylosus (22.9%). Six NAS isolates were mecA-positive carrying staphylococcal cassette chromosome mec (SCCmec) II (n=1), SCCmec IV (n=1), SCCmec V (n=2), or non-typeable SCCmec element (n=2). While two mecA-positive Staphylococcus epidermidis isolates from farm workers had SCCmec II and IV, a mecA-positive S. epidermidis isolate from broiler and a Staphylococcus haemolyticus isolate farm environment carried SCCmec V. The occurrence of multidrug resistance was observed in 48.1% (104/216 isolates) of NAS isolates with high resistance rates to β-lactams (>40%) and fusidic acid (59.7%). Fluoroquinolone resistance was confirmed in 59 NAS isolates (27.3%), and diverse mutations in the quinolone resistance determining regions of gyrA, gyrB, parC, and parE were identified. These findings suggest that NAS in broiler farms may have a potential role in the acquisition, amplification, and transmission of antimicrobial resistance.

Bacteriocin-Producing Escherichia coli Q5 and C41 with Potential Probiotic Properties: In Silico, In Vitro, and In Vivo Studies.

Commensal bacteriocin-producing Escherichia coli are of interest for possible use as probiotics to selectively control the spread of pathogenic bacteria. Here, we evaluated the biosafety and efficacy of two new bacteriocin-producing E. coli strains, Q5 (VKM B-3706D) and C41 (VKM B-3707D), isolated from healthy farm animals. The genomes of both strains were sequenced, and genes responsible for the antagonistic and colonization abilities of each strain were identified. In vitro studies have shown that both strains were medium-adhesive and demonstrated antagonistic activity against most enteropathogens tested. Oral administration of 5 × 108 to 5 × 1010 colony-forming units of both strains to rats with drinking water did not cause any disease symptoms or side effects. Short-term (5 days) oral administration of both strains protected rats from colonization and pathogenic effects of a toxigenic beta-lactam-resistant strain of E. coli C55 and helped preserve intestinal homeostasis. Taken together, these in silico, in vitro, and in vivo data indicate that both strains (and especially E. coli Q5) can be potentially used for the prevention of colibacillosis in farm animals.

Review of Green Technologies Use by Farmers: Implications for Environmental Sustainability in Nigeria

Background: The study reviewed green technologies used by farmers. It specifically reviewed what green technology is all about in farming; green technologies used by farmers and its role on environmental sustainability and challenge to use of green technology in farming.
 Materials and Methods: The author reviewed books, journals, bulletin theses, dissertations, research reports etc.
 Results: Findings show that solar photovoltaic, wind energy, biofuel, biogas, biomass, organic farming, integrated pest management, mixed farming, etc. are the most common green technologies use by farmers in Nigeria. The findinds further showed that the adverse effect of conventional technology and modern farming practice on the natural environment were environmental degradation, global warming, climate change, reduction in biodiversity, destruction of soil health and natural habitat, economic loss to farmers, which can be addressed by green technologies use with its potentials of healing damaged natural environments and ensuring profitability as cost of production is reduced by 67% and sustainability of environment in the production process. The benefits of green technologies are quality natural environment, healthy farm produce and sustainable biodiversity while challenges to its use are cost intensive, lack of technical know-how, illiteracy of the farmers, inadequate information on the potentials and limitation of its application. Green technology is the path of rapid agricultural development and welfare improvement as it increases profitability and health of farmers and guarantees environmental sustainability. The study recommends policy measures promoting use of green technologies by farmers for environmental sustainability and profit maximization.

Soil health and synergy of ecological determinants of green cocoa productivity in different soil ecotypes in Ghana

IntroductionSoil health is critical for the efficient management of soil fertility and crop yield in “green” cocoa (GC) (Theobroma cacao L.) agroforestry systems. However, knowledge about agroecosystem factors that affect healthy soil productivity in “green” cocoa agroforestry systems is patchy in West Africa. Based on organic cocoa (OC) and conventional cocoa (CC) agroforestry systems in Ghana, this study examined the soil health and synergy of ecological factors that determine the yield of GC.MethodsUsing multi-stage random sampling, 11 CC and 11 OC farms were sampled from three soil types (ferralsols, lixisols, and leptosols) within selected agroecological zones. Socioeconomic and farm data, including bulked soil samples, were collected at 0–30 cm depth for analysis of soil chemical and physical properties.ResultsThe results showed intricate relationships between the ecological factors and the yield of GC (1.07 t ha−1), which comprised dry beans of OC (1.24 t ha−1) and CC (0.89 t ha−1). The green cocoa yield increased for fields owned by female farmers and for native farmers who inherited or outrightly owned farmlands. The cocoa yield was also positively related to physicochemical factors such as soil organic carbon (0.21%), pH (5.8), and carbon–nitrogen ratio (40.8%). The carbon–nitrogen ratio and pH together exerted the highest positive influence (0.62%) on the yield. Biological factors such as plant density (>7 cocoa trees per 23.4 m2) and black pod rots reduced the cocoa yield.DiscussionThis study provides comprehensive empirical determinants of green cocoa productivity and offers a more reliable estimate of cocoa plant density. The findings suggest that Ghana's cocoa can be much greener if stakeholders promote healthy farm soil productivity and empower women who engage in soil organic carbon-conserving agroforestry.

Plant and soil health in organic strawberry farms – Greater importance of fungal trophic modes and networks than α-diversity of the mycobiome

The mycobiome composition and health status of host plants are closely related. These relationships should be studied and recognized, as they may form the basis of future sustainable agriculture. The aim of this study was to select relevant biological indicators for monitoring soil microbial diversity by revealing characteristic differences between the mycobiomes of various soil compartments (bulk and rhizosphere) and plant niches (roots and shoots) from 13 healthy and diseased organic strawberry farms. The results of this study show that α-diversity of the mycobiome is less important in shaping plant health than fungal trophic mode composition. Healthy soil niches and roots contain less core taxa, but more differentially abundant taxa, than unhealthy ones. The results also suggest that plant health was shaped by more than just the most abundant fungal taxa, indicating that rare taxa should not be neglected in the analysis of mycobiome structure. We also demonstrate that fungal communities in the soil and plant niches from healthy strawberry farms create more stable and steady networks as compared to those from unhealthy farms. Finally, we show that the mycobiome roots-shoot axis is an important trait to consider in unhealthy plants from strawberry farms.

Plasmid-borne mobile colistin resistance (MCR-1) in healthy humans and poultry

Background and aim: The emergence of antimicrobial-resistant bacteria has become a menace to global public health and socio-economic development. Colistin is used as the last resort antibiotic in refractory infections. However, resistant isolates are reported frequently. The aim of this study was to find mcr genes in healthy people poultry farms in Sanandaj, west of Iran. Methods and materials: In this study, faecal samples were collected from two groups of humans and poultry. Colistin-containing screening media as well as colistin-EDTA-containing medium to screen plasmid-mediated colistin resistance. PCR for prevalent mcr genes was performed. Antimicrobial susceptibility testing was done for MCR-harboring isolates. Results: In this study, no colistin-resistant bacteria were isolated from poultry samples (0%) while two human E. coli isolates showed resistance to colistin (0.59%). Of which, one isolate was inhibited by EDTA and harboured the MCR-1 variant. The MIC for colistin was 16 mg/L. Conclusion: The results indicated the low prevalence of this gene in healthy individuals and the poultry industry. To the best of our knowledge, this is the first report of MCR in healthy individuals from Iran.

Immune Response Evaluation in Commercial Turkeys Affected with Clostridial Dermatitis.

Clostridial dermatitis (CD), caused by Clostridium septicum and Clostridium perfringens, is an economically important emerging disease of turkeys characterized by sudden deaths and necrotic dermatitis. Immune responses in CD-affected commercial turkeys are poorly understood. In the present study, C. septicum was isolated from CD-affected commercial turkeys during a recent outbreak, and the tissues (skin, muscle, and spleen) were collected and analyzed for immune gene expression, along with samples from clinically healthy birds. The results showed that CD-affected turkeys had significantly higher levels of IL-1β, IL-6, IFNγ, and iNOS transcripts in the skin, muscle, and spleen tissues compared to healthy birds. Affected turkeys also had a significantly elevated transcription of toll-like receptor (TLR21) gene in the skin and spleen tissues, suggesting a role for this receptor in the immune recognition. The expression of IL-4 and IL-13 genes in the spleen and muscle was also significantly higher in the affected birds. Additional birds from the same affected and healthy farms examined for serology revealed that the CD-affected turkeys had significantly higher levels of serum IgM and IgY antibodies. Furthermore, in vitro stimulation of MQ-NCSU macrophages with C. septicum led to a significant transcriptional upregulation of IL-1β and IFNγ genes, while the IL-10 gene expression was downregulated. The surface expression of MHC-II protein and cellular production of nitric oxide were also significantly increased in the C. septicum-stimulated macrophages, indicating cellular activation. Collectively, our findings suggest that the host responses in CD-affected turkeys involve a robust inflammatory response as well as a response mediated by IL4/IL-13 cytokines that may aid in antibody-mediated immunity.

Impacts of Domestication and Veterinary Treatment on Mobile Genetic Elements and Resistance Genes in Equine Fecal Bacteria.

Antimicrobial resistance in bacteria is a threat to both human and animal health. We aimed to understand the impact of domestication and antimicrobial treatment on the types and numbers of resistant bacteria, antibiotic resistance genes (ARGs), and class 1 integrons (C1I) in the equine gut microbiome. Antibiotic-resistant fecal bacteria were isolated from wild horses, healthy farm horses, and horses undergoing veterinary treatment, and isolates (9,083 colonies) were screened by PCR for C1I; these were found at frequencies of 9.8% (vet horses), 0.31% (farm horses), and 0.05% (wild horses). A collection of 71 unique C1I+ isolates (17 Actinobacteria and 54 Proteobacteria) was subjected to resistance profiling and genome sequencing. Farm horses yielded mostly C1I+ Actinobacteria (Rhodococcus, Micrococcus, Microbacterium, Arthrobacter, Glutamicibacter, Kocuria), while vet horses primarily yielded C1I+ Proteobacteria (Escherichia, Klebsiella, Enterobacter, Pantoea, Acinetobacter, Leclercia, Ochrobactrum); the vet isolates had more extensive resistance and stronger PC promoters in the C1Is. All integrons in Actinobacteria were flanked by copies of IS6100, except in Micrococcus, where a novel IS5 family element (ISMcte1) was implicated in mobilization. In the Proteobacteria, C1Is were predominantly associated with IS26 and also IS1, Tn21, Tn1721, Tn512, and a putative formaldehyde-resistance transposon (Tn7489). Several large C1I-containing plasmid contigs were retrieved; two of these (plasmid types Y and F) also had extensive sets of metal resistance genes, including a novel copper-resistance transposon (Tn7519). Both veterinary treatment and domestication increase the frequency of C1Is in equine gut microflora, and each of these anthropogenic factors selects for a distinct group of integron-containing bacteria. IMPORTANCE There is increasing acknowledgment that a "one health" approach is required to tackle the growing problem of antimicrobial resistance. This requires that the issue is examined from not only the perspective of human medicine but also includes consideration of the roles of antimicrobials in veterinary medicine and agriculture and recognizes the importance of other ecological compartments in the dissemination of ARGs and mobile genetic elements such as C1I. We have shown that domestication and veterinary treatment increase the frequency of occurrence of C1Is in the equine gut microflora and that, in healthy farm horses, the C1I are unexpectedly found in Actinobacteria, while in horses receiving antimicrobial veterinary treatments, a taxonomic shift occurs, and the more typical integron-containing Proteobacteria are found. We identified several new mobile genetic elements (plasmids, insertion sequences [IS], and transposons) on genomic contigs from the integron-containing equine bacteria.

High Prevalence of Clonal Complex 398 Methicillin-Susceptible and -Resistant Staphylococcus aureus in Pig Farms: Clonal Lineages, Multiple Drug Resistance, and Occurrence of the Staphylococcal Cassette Chromosome mec IX

High prevalence of livestock-associated methicillin-susceptible and -resistant Staphylococcus aureus (LA-MSSA and LA-MRSA, respectively) in livestock farms, particularly pig farms, is an increasingly serious threat to food safety and public health. In this study, 173 S. aureus (84 MRSA and 89 MSSA) isolates from healthy pigs, farm environments, and farm workers in Korea were examined to determine the (1) genetic diversity of S. aureus isolates (sequence type [ST], spa, and agr types), (2) staphylococcal cassette chromosome mec (SCCmec) types of MRSA isolates, and (3) multidrug resistance (MDR) phenotypes of MRSA and MSSA isolates. Clonal complex 398 (CC398) genotypes of MRSA and MSSA isolates, particularly CC398-spa type t571-agr I lineages, displaying MDR phenotypes were highly prevalent in pig farms. High prevalence of CC398-t571 MRSA and MSSA was more frequently associated with weaning piglets and growing pigs. Moreover, the same clonal lineages of S. aureus isolates colonized both pigs and farm workers, suggesting the transmission of antimicrobial-resistant CC398 MRSA and MSSA between pigs and humans in the pig farms. Furthermore, two dominant SCCmec types, SCCmec V and SCCmec IX, were identified in CC398 MRSA isolates colonizing healthy pigs. To the best of our knowledge, this is the first report of a CC398 LA-MRSA isolate carrying SCCmec IX in Korea. Collectively, these results suggest widespread distribution of the CC398 lineage among MRSA and MSSA isolates in pigs, farm environments, and farm workers in Korea.

An investigative study on the zoonotic potential of Helicobacter pylori

BackgroundHelicobacter pylori is one of the most common bacterial infections and is widespread globally. It causes a variety of gastrointestinal disorders, though a great proportion of infections are asymptomatic. A total of 143 fresh stool samples were collected from apparently healthy farm and pet animals (43 cattle, 50 buffaloes, 50 sheep, 50 dogs, and 50 cats), in addition to 768 human stool samples. The samples were examined using stool antigen and rapid antibody tests, and further confirmation of glmM “human antigen-positive samples and animal milk samples” was conducted by polymerase chain reaction (PCR).ResultsThe prevalence rates of H. pylori infection in animals were 22.2% and 16% in antibody and stool antigen tests, respectively. The detection rates were 28%, 24%, 12%, 10%, and 4.7% in cats, dogs, buffaloes, sheep, and cattle, respectively. On the other hand, the prevalence rate of H. pylori infection in human stool samples was 74.8%, and a statistically significant association was observed between prevalence and several factors, such as sex, age, and locality. PCR was performed to detect the glmM gene of H. pylori, and this gene was found in 21 of 27 human antigen-positive samples and 5 of 13 animal milk samples.ConclusionsH. pylori was detected in both human and animal samples. Furthermore, glmM was found in milk and human samples. Our findings suggest that pet and farm animals could transmit H. pylori infection to humans.