Health Effects Of Environmental Pollutants Research Articles

Environmental pollution, trade openness and the health of middle-aged and elderly people: an analysis of threshold effect based on data from 111 prefecture-level cities in China

BackgroundEnvironmental pollution seriously endangers people’s physical and mental health, especially the health of middle-aged and elderly people. Environmental pollution, trade openness, and population health are interconnected. Environmental pollution may have a nonlinear impact on health, and the impact of trade openness on the health effects of environmental pollution may not be a simple strengthening or weakening effect. However, few studies have used threshold effects model to explore the nonlinear mechanisms of environmental pollution’s impact on health in China. As a result, this study incorporates trade openness into the research framework on the health effects of environmental pollution, aiming to study the mechanism of environmental pollution on health.MethodsUsing the China Health and Retirement Longitudinal Study (CHARLS) data from 2013 to 2020 and the data of 111 prefecture-level cities in China, we combine two-way fixed-effects models and threshold models to explore the effects of environmental pollution on the health of middle-aged and elderly people and the role of trade openness in the path of environmental pollution affecting health.ResultsEnvironmental pollution impairs the health of middle-aged and elderly people, and there is a single threshold effect and regional heterogeneity in this negative impact. Trade openness has the effect of first weakening and then strengthening in the inhibitory effect of environmental pollution on health.ConclusionThe negative impact of environmental pollution on health has regional heterogeneity, and there is a nonlinear relationship between environmental pollution and the health of middle-aged and elderly people. The health effect of environmental pollution is mainly long-term effect, and trade openness has a threshold effect on the impact of environmental pollution on health. Therefore, instead of adopting a one-size-fits-all policy, environmental and economic policies should be customized according to the degree of environmental pollution, trade openness, and regional variations, so as to safeguard the health of middle-aged and elderly individuals through effective environmental governance.

Associations between blood manganese levels and sarcopenia in adults: insights from the National Health and Nutrition Examination Survey.

While increasing concerns arise about the health effects of environmental pollutants, the relationship between blood manganese (Mn) and sarcopenia has yet to be fully explored in the general population. This study aims to investigate the association between blood manganese (Mn) levels and sarcopenia in adults. In our study, we evaluated 8,135 individuals aged 18-59 years, utilizing data from the National Health and Nutrition Examination Survey (NHANES) spanning 2011 to 2018. We employed generalized additive model (GAM) to discern potential non-linear relationships and utilized the two-piecewise linear regression model to probe the association between blood Mn levels and sarcopenia. After adjusting for potential confounders, we identified non-linear association between blood Mn levels and sarcopenia, with an inflection point at 13.45 μg/L. The effect sizes and the confidence intervals on the left and right sides of the inflection point were 1.006 (0.996 to 1.048) and 1.082 (1.043 to 1.122), respectively. Subgroup analysis showed that the effect sizes of blood Mn on sarcopenia have significant differences in gender and different BMI groups. Our results showed that a reverse U-shaped curve between blood Mn levels and sarcopenia, with an identified the inflection point at blood Mn level of 13.45 μg/L.

Health Literacy and Environmental Risks Focusing Air Pollution: Results from a Cross-Sectional Study in Germany.

(1) Background: Environmental risks such as air pollutants pose a threat to human health and must be communicated to the affected population to create awareness, such as via health literacy (HL); (2) Methods: We analyzed HL in the context of environmental health risks, including sources of information and prior knowledge, in a sample from the German general population using Kendall's rank correlations, regression analyses, and explorative parallel mediation analysis; (3) Results: The survey included 412 German participants aged between 18 and 77. HL was found to be problematic to inadequate. The internet, family and friends, and newspapers were the most frequently cited sources of information. Mobile apps were mostly unknown but were requested by sample subjects. Although subjects expressed environmental concerns and exhibited rather good levels of knowledge, the majority perceived no risk to human health and rated air quality quite positively. Knowledge on particulate matter, the term "ultrafine particles", and protective measures was found to be rather low. HL was associated with the use of newspapers and commercials as sources of information. The relationship between age and HL is fully mediated by the use of newspapers and information from TV commercials; (4) Conclusions: HL should be promoted by raising awareness of the health effects of environmental pollutants. In particular, the information channels preferred by the affected population should be used and further information opportunities such as apps should be publicized, e.g., through campaigns. An improved HL can assist policy makers in creating a healthier environment by empowering individuals to become more environmentally aware and protect their own health. This, in turn, has the potential to reduce health-related costs.

Global environmental changes and the epithelial barrier hypothesis

The global burden of noncommunicable diseases (NCD) has seen astrong increase in recent decades and attributable to the influence of environmental factors. For amultitude of diseases an association with epithelial barrier damage has been reported. This article provides an overview of the health effects of environmental pollution in the context of the epithelial barrier hypothesis of Cezmi Akdis. Additionally, exemplary mechanisms of abarrier damage are described. Finally, possible preventive and therapeutic consequences are discussed. The PubMed database was searched for the relevant topics and selected literature was reviewed. Awide variety of substances can damage the epithelial barriers of the skin, lungs and gastrointestinal tract. The rise in the prevalences of atopic diseases could (partly) be due to an increased exposure to barrier-damaging substances, such as particulate matter and laundry detergents. Apossible pathogenetic mechanism is the initiation and maintenance of an immune response by subepithelial penetration of microorganisms through damaged epithelia. Based on the epithelial barrier hypothesis new therapeutic and prevention strategies can be developed. The regulation of hazardous chemicals and the fight against environmental pollution and climate change are necessary to reduce the burden of disease.

Comprehensive Hygienic Assessment of Anthropogenic Load on the Territory of the Voronezh Region

Introduction: A comprehensive assessment of adverse human health effects of environmental pollutants found in residential areas has been carried out in the Voronezh Region. Objective: To conduct a comprehensive hygienic assessment of the anthropogenic load on the territory of the Voronezh Region. Materials and methods: We analyzed the results of ambient air, drinking water, and soil quality monitoring collected by the Center for Hygiene and Epidemiology in the Voronezh Region in 2018–2022 for compliance with the maximum permissible concentrations specified in Regulations SanPiN 1.2.3685–21. The complex load was estimated in accordance with method guidelines MR 01-19/17-17. Results: We established that pollution of ambient air in residential areas contributed the most (69.8 %) to the total anthropogenic load in the town Pavlovsk; drinking water – to that in the Semiluksky district (36.5 %), and soil – in the Rossoshansky district (10.5 %). In the city of Voronezh, the level of complex anthropogenic load exceeded both the regional and district averages. The problem of ambient air pollution dominates in all districts; it should be noted, however, that air sampling is carried out only in urban areas and urban-type settlements. In rural areas, effects of agriculture-associated nitrate pollution of drinking water is more pronounced. The impact of soil pollution is more pronounced in urban residential areas. At the same time, the excess of maximum permissible concentrations of soil pollutants were registered neither in rural nor in urban areas over the study period. Conclusion: In Voronezh, the level of complex anthropogenic load exceeds the average values estimated for the region and districts. Ambient air pollution with vehicle and industrial emissions make the greatest contributions to this load both on the territory of Voronezh and in the districts. Nitrate pollution of potable water associated with agriculture is a challenge in rural areas.

Can the development of renewable energy in China compensate for the damage caused by environmental pollution to residents' health?

China's rapid economic growth in recent decades has caused a growing problem of environmental pollution, which negatively impacts the physical and mental health of residents. In recent years, renewable energy has emerged as a promising solution to alleviate environmental pollution and improve residents' well-being. However, it is unknown whether renewable energy development can counterbalance the health impacts of environmental pollution. Therefore, we conducted a study using data from the China Family Panel Studies (CFPS) to examine the impact of environmental pollution and renewable energy on the health of 20,694 residents. Our analysis showed that renewable energy development can partially offset the negative health effects of environmental pollution. Specifically, we found that a 1% increase in environmental pollution is linked to an average decrease of 0.0911% in physical health (PHY) and 0.0566% in mental health (MEN), whereas each 1% rise in renewable energy corresponds to an average increase of 0.2585% in PHY and 0.1847% in MEN. These positive effects apply to male and female residents, urban and rural residents, young and middle-aged adults, and people with low, medium, and high levels of education. These findings are significant for decision-makers striving to improve Chinese residents' physical and mental health by considering the specific impact of renewable energy and comprehensive environmental pollution.

Population Health Risk and Harm Indicators in the System of New Mechanisms for Air Quality Monitoring and Management

Introduction: The article dwells on major legislative and methodical aspects related to the latest update of the system for air quality management in the country. Objective: To summarize the experience in applying the health risk assessment procedure to the system of new mechanisms for ambient air quality monitoring and management. Materials and methods: The study relied on the results obtained by summary estimates of dispersion of emissions from 1.5 to 6.5 thousand sources per city and health risk assessment in twelve cities included in the Federal Clean Air Project. We also analyzed the results of biomedical studies (about two thousand chemical, immunological, and clinical tests done in 2020–2022) in and outside the zones of influence of mining and chemical industries. Results: Health risks were estimated as unacceptable in the cities included in the experiment on introducing new emission control technologies. In some cases, risks were rated as “very high” for diseases of the respiratory system, blood and blood-forming organs, musculoskeletal system, etc. We substantiated the lists of priority pollutants for emission monitoring and quoting and the lists of priority objects generating unacceptable risks. We demonstrate that the evaluation of health damage as an indicator of realized risks under certain circumstances can serve as yet another evidence of adverse health effects of environmental pollution. We suggest a fundamental algorithm for including the assessment of human health risks and damages into the system of air quality management for elaboration of the existing regulatory and methodological framework of monitoring and standardization. Conclusions: The procedures of assessing health risks and damages are a significant component of air quality management. Their results ensure adequacy of the monitoring programs to the current situation, make managerial actions more targeted and precise, and guarantee optimal achievement of the major goal to preserve public health through safe and comfortable environment.

Health Effects of Environmental Pollutants on Workers in the Libyan Plastic Factories, Part B: Based on Molecular Levels Characteristics

This study aimed to disclose the reasons for the health damage and heritable genetic mutations among workers in plastic manufactories due to negative environmental impacts. Moreover, this study may constitute an initial database that expresses the negative impact on the environment in the plastics factories that may have a negative impact on workers. To study the effects of long-term exposure to plastic and its solvent vapors during plastic manifesting on the blood DNA contents, blood samples were withdrawn from volunteers working in a plastics factory in Kasr Alakhyar city, Libya. Six different samples were collected from Workers exposed to plastic vapor for different periods as follows, control (did not expose to plastic vapor), one-year exposure, three years' exposure, six years' exposure, seven years' exposure, and twelve years' exposure.
 Blood samples were collected and subjected to molecular analysis using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR). Molecular analyses were performed to assess the genotoxicity effect of plastic vapor on the molecular level. On the molecular level, the DNA of the six aforementioned volunteers was extracted and subjected to RAPD analysis. The results showed several differences in the banding patterns was recorded between the control volunteer and volunteer and those who exposed to plastic and its solvents vapor for different exposure periods. These changes in the banding patterns suggested that a modification in the genomic DNA occurred in form of modifications in the nitrogen bases of the genomic DNA as a response to the exposure to plastic vapor for a different time exposure.

Perception of Health Effects of Environmental Pollutants Amongst Gas-Plant workers and Residents of Selected Local Government Areas of Delta State.

Perception of Health Effects of Environmental Pollutants Amongst Gas-Plant workers and Residents of Selected Local Government Areas of Delta State.

Health Effects of Environmental Pollutants on Workers in The Libyan Plastic Factories, Part A: Based on Biochemical Analysis

This study aimed to disclose the reasons for the health damage and heritable genetic mutations among workers in plastic manufactories due to negative environmental impacts. Moreover, this study may constitute an initial database expressing the negative impact on the environment in the plastics factories that may have a negative impact on workers. To study the effects of long-term exposure to plastic and its solvent vapors during plastic manifesting on the blood protein contents, blood samples were withdrawn from volunteers working in a plastics factory in Kasr Alakhyar City, North-west Libya. Six different samples were collected from Clarkes exposed to plastic vapor for different periods as follows, control (did not expose to plastic vapor), one-year exposure, three years exposure, six years exposure, seven years exposure, and twelve years exposure. Blood samples were collected and subjected to protein electrophoresis and isoenzymes analysis for esterase and peroxidase. The results of protein electrophoresis indicated that a total number of 14 protein bands were resolved on polyacrylamide gel electrophoresis ranging from 16.5 KDa to 105 KDa. Five bands out of the resulted from 14 bands were considered common bands with molecular weights of 105, 98, 85, 63, and 16.5 KDa. (i.e. these bands were presented in all the six blood samples under investigation). The results also showed that too many changes were recorded in the protein banding patterns between the control volunteer and those who were exposed to plastic and its solvents vapor for different exposure periods. These changes in proteins suggested that a modification in gene excretion occurred as a response to the different exposure times to plastic during manifesting. Also, in esterase and peroxidase isozymes changes in the banding patterns between the control volunteer and those who were exposed to plastic and its solvents vapor for different exposure periods. These changes in proteins suggested that a modification in gene excretion occurred in the genes conferring these isozymes as a response to the different exposure periods to plastic during manifesting.

Using phenome-wide association studies to examine the effect of environmental exposures on human health

The field of environmental epidemiology has been using “-omics” technologies, including the exposome, metabolome, and methylome, to understand the potential effects and biological pathways of a number of environmental pollutants. However, the majority of studies have focused on a single disease or phenotype, and have not systematically considered patterns of multimorbidity and whether environmental pollutants have pleiotropic effects. These questions could be addressed by examining the relation between environmental exposures and the phenome – the patterns and profiles of human health that individuals experience from birth to death. By conducting Phenome Wide Association Studies (PheWAS), we can generate new hypotheses about new or poorly understood exposures, identify novel associations for established toxicants, and better understand biological pathways affected by environmental pollutants. In this article, we provide a conceptual framework for conducting PheWAS in environmental epidemiology and summarize some of the advantages and challenges to using the PheWAS to study environmental pollutant exposures. Ultimately, by adding the PheWAS to our “-omics” toolbox, we could substantially improve our understanding of the potential health effects of environmental pollutants.

Directions for and prospects of the Environmental Health Study in Korean National Industrial Complexes (EHSNIC): A proposal for the third phase of the EHSNIC.

The Environmental Health Study in the Korean National Industrial Complexes (EHSNIC) is a project that aims to monitor the exposure and health effects of environmental pollution among residents of national industrial complexes, as well as propose appropriate environmental health measures. Since its launch in 2003, this project has been initiated in eight national industrial complexes. Currently, it is necessary to review the accomplishments and limitations of the phases 1 and 2 of this project, and establish the direction of the upcoming the phase 3. Thus, the present study has developed principles and goals for the phase 3, considering the rationale and justification of the EHSNIC, and presented specific research contents accordingly. In the phase 3, it is important to improve the methods for exposure assessment and evaluation of health effects, in order to identify clearly the association between the pollutants released from industrial complexes and their health impacts, to develop and to reinforce communication strategies to promote participation of residents of communities near industrial complexes. Nonetheless, it is also important to maintain the basic goal of continuously monitoring the level of exposure to and health effects of environmental pollutants.

Health effects of environmental pollution in population living near industrial complex areas in Korea.

Several epidemiological studies have reported an association between environmental pollution and various health conditions in individuals residing in industrial complexes. To evaluate the effects of pollution from industrial complex on human health, we performed a pooled analysis of environmental epidemiologic monitoring data for residents living near national industrial complexes in Korea. The respiratory and allergic symptoms and the prevalence of acute and chronic diseases, including cancer, were used as the outcome variables for health effects. Multiple logistic regression analysis was used to analyze the relationship between exposure to pollution from industrial complexes and health conditions. After adjusting for age, sex, smoking status, occupational exposure, level of education, and body mass index, the residents near the industrial complexes were found to have more respiratory symptoms, such as cough (odds ratio [OR], 1.18; 95% confidence interval [CI], 1.06 to 1.31) and sputum production (OR, 1.13; 95% CI, 1.03 to 1.24), and symptoms of atopic dermatitis (OR, 1.10; 95% CI, 1.01 to 1.20). Among residents of the industrial complexes, the prevalence of acute eye disorders was approximately 40% higher (OR, 1.39; 95% CI, 1.04 to 1.84) and the prevalence of lung and uterine cancer was 3.45 times and 1.88 times higher, respectively, than those among residents of the control area. This study showed that residents living in the vicinity of industrial complexes have a high risk of acute and chronic diseases including respiratory and allergic conditions. These results can be used as basic objective data for developing health management measures for individuals residing near industrial complexes.

Using science to conquer haze: an interview with Tong Zhu

Abstract With a doctorate degree from the University of Wuppertal in Germany (1991), Tong Zhu is a Cheung Kong Chair Professor of Environmental Sciences at Peking University (PKU). He is a leading scientist in atmospheric pollution study and has been advising the Chinese government on controlling air pollution in the event of major international activities in China, including the 2008 Beijing Olympics. He served as a co-chair of the scientific steering committee of International Global Atmospheric Chemistry (IGAC) and now is the chair of the Expert Panel of the National Natural Science Foundation of China's Major Research Program, ‘Fundamental researches on the formation and response mechanism of air pollution complex in China’. His research is focused on air pollution related chemical reactions, health effects of environmental pollution, megacity and regional air pollution control, and the air surface exchange and global biogeochemistry. To better understand the current status of the atmospheric pollution research in China and its contribution to air pollution control, the National Science Review made an exclusive interview with Prof. Zhu.

Does wastewater discharge have relations with increase of Turner syndrome and Down syndrome?

The purpose of this study is to examine whether water and air pollutants have a relationship with an increase in the genetic disorders Turner syndrome and Down syndrome, which are caused by congenital chromosomal abnormalities, and to generate a hypothesis about the genetic health effects of environmental pollutants. A panel regression based on random effect was conducted on Korea’s metropolitan councils from 2012 to 2014. The dependent variable was the number of Turner syndrome and Down syndrome cases, and the main independent variables were those regarding the water and air pollution. Air pollutants did not have a significant impact on the number of Turner syndrome and Down syndrome cases; however, the increase in number of wastewater discharge companies did have a significant relationship with the number of cases. The more the number of wastewater discharge companies, the more the number Turner syndrome and Down syndrome cases were observed. Therefore, scientific investigation on water and air pollutants in relation with genetic health effects needs to be performed.

Environmental Pollution Effects on Mortality in an Industrial and a Non-industrial Municipality, in Voiotia Prefecture, Greece

In the Oinofita region, there was strong evidence of air pollution and hexavalent chromium (Cr(VI))-contaminated drinking water due to the increased number of industries, in contrast to the closely-located region of Arachova, which served as a control. To examine the health effects of environmental pollution, we performed an ecological study to compare the all-cause and cause-specific mortality. We considered the registered citizens of both study areas during an 11-year period (1999-2009) and we used the direct method of standardization to calculate the age, gender and period standardized rates (ASRs) and the standardized rate ratios (SRRs), using the greater Prefecture of Voiotia as the standard population. Statistically significantly higher rates of all-cause mortality (SRR = 1.22, 95% confidence intervals (CI) 1.1-1.4), cardiovascular (SRR = 1.36, 95% CI 1.1-1.7) and cerebrovascular diseases (SRR = 2.93, 95% CI 1.8-4.8) were identified for Oinofita compared to Arachova. Furthermore, suggestive results were found for cardiopulmonary diseases among males (SRR = 1.52, 95% CI 1.0-2.4) and leukaemias (SRR = 4.65, 95% CI 0.9-25.3). Elevated SRRs, not statistically significant though, were also observed for respiratory diseases, all cancers and specific cancer sites (lip, oral cavity and pharynx, liver, stomach, pancreas, lung, prostate, colon and kidney and other genitourinary organs among females). Increased mortality rates in the Oinofita region support the hypothesis of adverse health effects association with air pollution and Cr(VI)-contaminated drinking water. Further studies are needed to determine if this association is causal and to establish preventive guidelines and public health recommendations.

Long-term Neurotoxic Effects of Early-life Exposure to Tetrachloroethylene-contaminated Drinking Water

BackgroundTetrachloroethene (PCE) is a common environmental and occupational contaminant and an acknowledged neurotoxicant. From 1968 through 1983, widespread contamination of public drinking water supplies with PCE occurred in the Cape Cod region of Massachusetts. The source of the contamination was a vinyl liner applied to the inner surface of water distribution pipes. ObjectivesA retrospective cohort study (the Cape Cod Health Study) was undertaken to examine possible health consequences of early-life exposure to PCE-contaminated drinking water. This review describes the study methods and findings regarding the effects of prenatal and childhood exposure on neurologic outcomes during early adulthood, including vision, neuropsychological functioning, brain structure, risky behaviors, and mental illness. The review also describes the strengths and challenges of conducting population-based epidemiologic research in this unique setting. MethodsParticipants were identified by cross-matching birth certificates and water system data. Information on health outcomes and confounding variables was collected from self-administered surveys (n = 1689), neuropsychological tests (n = 63), vision examinations (n = 63), and magnetic resonance imaging (n = 42). Early-life exposure to PCE was estimated using a leaching and transport model. The data analysis compared the occurrence of each health outcome among individuals with prenatal and early childhood PCE exposure to unexposed individuals while considering the effect of confounding variables. FindingsThe study found evidence that early-life exposure to PCE-contaminated drinking water has long-term neurotoxic effects. The strongest associations were seen with illicit drug use, bipolar disorder, and post-traumatic stress disorder. Key strengths of the study were availability of historical data on affected water systems, a relatively high exposure prevalence and wide range of exposure levels, and little confounding. Challenges arose mainly from the historical nature of the exposure assessments. ConclusionsThe Cape Cod Health Study demonstrates how scientists can take advantage of unique “natural experiments” to learn about the health effects of environmental pollution. This body of work has improved our understanding of the long-term health effects of early-life exposure to this common environmental contaminant and will help risk assessors and policymakers ensure that drinking water supplies in the United States are safe for vulnerable populations.

Useful biomarkers for assessing the adverse health effects of PCBs in allergic children: pediatric molecular epidemiology.

The incidences of childhood allergies have been increasing in recent years in many parts of the world. The development of allergic disorders is attributed to a complex series of interactions between individuals' genetic backgrounds and their immune and psychoneurotic responses to environmental factors. Among the various possible environmental causes of childhood allergies, the early exposure of developing infants to air pollutants and the presence of persistent chemical pollutants such as pesticides have been suggested most frequently. Therefore, it is very important to obtain epidemiological evidence of direct associations between clearly defined adverse health effects and exposure to low levels of pollutants. However, there are no useful biomarkers for assessing such associations. Thus, we planned to establish reliable health-related biomarkers that could be used to investigate these relationships in children. The serum concentrations of several sub-types of polychlorinated biphenyl (PCB) congeners were found to be significantly correlated with interleukin (IL)-8 mRNA expression among asthmatic children. In addition, IL-22 mRNA expression was found to be particularly useful for detecting the effects of environmental pollutants, especially PCB congeners, in a sub-population of vulnerable children who exhibited positive immunoglobulin E (IgE) responses to milk or egg. Furthermore, we detected significant differences in IL-22 mRNA expression between the IgE-negative non-asthmatic subjects and the asthmatic children who exhibited positive IgE reactions toward egg or milk. In conclusion, IL-8 and IL-22 mRNA expressions could be useful biomarkers for detecting sub-populations of children who are particularly vulnerable to the adverse health effects of environmental pollutants, especially PCBs.

Success of 2013-2020 World Health Organization action plan to control non-communicable diseases would require pollutants control.

To control diabetes epidemic and NCDs, WHO has been promoting its action plans since 2000, but diabetes epidemic is continuing. New action plan for 2013-2020 aim to reduce shared risk factors of NCDs, such as tobacco use, unhealthy (i.e., high fat) diet, physical inactivity and harmful use of alcohol and is strengthening the risk reduction measures, such as taxing on tobacco, alcohol and sugary beverages, banning advertisements of unhealthy foods. However it might fail because it did not consider the contribution of environmental pollution on the pathogenesis of diabetes and NCDs.

Nitration of the Birch Pollen Allergen Bet v 1.0101: Efficiency and Site-Selectivity of Liquid and Gaseous Nitrating Agents

Nitration of the major birch pollen allergen Bet v 1 alters the immune responses toward this protein, but the underlying chemical mechanisms are not yet understood. Here we address the efficiency and site-selectivity of the nitration reaction of recombinant protein samples of Bet v 1.0101 with different nitrating agents relevant for laboratory investigations (tetranitromethane, TNM), for physiological processes (peroxynitrite, ONOO–), and for the health effects of environmental pollutants (nitrogen dioxide and ozone, O3/NO2). We determined the total tyrosine nitration degrees (ND) and the NDs of individual tyrosine residues (NDY). High-performance liquid chromatography coupled to diode array detection and HPLC coupled to high-resolution mass spectrometry analysis of intact proteins, HPLC coupled to tandem mass spectrometry analysis of tryptic peptides, and amino acid analysis of hydrolyzed samples were performed. The preferred reaction sites were tyrosine residues at the following positions in the polypeptide chain: Y83 and Y81 for TNM, Y150 for ONOO–, and Y83 and Y158 for O3/NO2. The tyrosine residues Y83 and Y81 are located in a hydrophobic cavity, while Y150 and Y158 are located in solvent-accessible and flexible structures of the C-terminal region. The heterogeneous reaction with O3/NO2 was found to be strongly dependent on the phase state of the protein. Nitration rates were about one order of magnitude higher for aqueous protein solutions (∼20% per day) than for protein filter samples (∼2% per day). Overall, our findings show that the kinetics and site-selectivity of nitration strongly depend on the nitrating agent and reaction conditions, which may also affect the biological function and adverse health effects of the nitrated protein.