Background Effective gender-based violence (GBV) interventions depend on precise tools to assess healthcare providers' knowledge, attitudes, and practices (KAP). This study aimed to develop and validate a culturally appropriate GBV questionnaire on the KAP of healthcare workers in India using a modified e-Delphi method. Methodology The study used a modified e-Delphi approach, conducted online, to validate a GBV questionnaire. The process included the following three rounds: content validation, quantitative validation, and finalization. It began with a literature review and questionnaire drafting in May 2022, followed by validation from September 2022 to December 2023. The expert panel included healthcare professionals, public health experts, and researchers. The questionnaire included the following four sections: demographic information, knowledge of GBV, attitudes toward GBV, and GBV-related practices. Results In Round 1, significant revisions were made to enhance relevance and clarity, including the addition of a "Don't Know" option and revisions to questions on gender roles and societal expectations. The quantitative validation of Round 2 revealed high scores for Importance (4.980), Usefulness (4.965), Relevance (4.965), Clarity (4.955), Simplicity (4.980), and Ambiguity (4.815), with all items retained and no significant modifications. In Round 3, consensus was achieved, finalizing the questionnaire with expert validation certificates, demonstrating strong reliability and contextual appropriateness. Conclusions The validated KAP questionnaire is a reliable tool for assessing healthcare providers' capacity to manage GBV. It can be used to inform and enhance interventions aimed at improving GBV management in healthcare settings.
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