Introduction: The attitude of clinicians will steer the direction of clinical care provided to patients with suicidal behavior. However, there is limited local data to suggest the attitude of health-care workers toward suicidal patients in the health-care settings. Objective: We aimed to assess the attitude of health-care professionals toward suicidal behavior and the factors influencing their attitude. Methods: A cross-sectional survey was conducted in a single tertiary health-care facility in northwest Malaysia, using a validated modified Suicide Opinion Questionnaire, distributed to medical doctors, nurses, and assistant medical officers. Results: A total of 487 health-care workers from 13 clinical departments participated in the study. We found that health-care professionals generally held unfavorable attitudes toward suicidal behavior, with 70.0% of the respondents generated low attitude scores in overall section. Independent factors that were found to be significantly associated with unfavorable attitudes toward suicidal behavior include religion (P = 0.027), education level (P = 0.007), and occupation (P = 0.007). Multivariate analysis with logistic regression showed that medical doctors were found to be 2.11 times more likely to hold unfavorable attitudes toward suicidal behavior as compared to nurses (95% confidence interval: 1.15–4.30, P = 0.009). Conclusion: Our findings highlighted the suicide stigma that is prevalent even among professional health-care workers in Malaysia. The unfavorable attitude toward suicidal behavior contrasted the elements of empathy and care that should be the core practice in health-care services. Therefore, we suggest for the implementation of training workshops incorporating interactive communication skills and reflective thinking to improve understanding and attitude toward suicidal behavior among health-care workers.