Healing Methods Research Articles

Autologous platelet-rich fibrin enhances skin wound healing in a feline trauma model

Trauma is a common cause of cutaneous surgical disease with an increased risk of secondary infection in cat clinics. Platelet-rich fibrin (PRF), a platelet and leukocyte concentrate containing multiple cytokines and growth factors, is known to accelerate the healing of wounds. However, how PRF affects wound healing in the cat trauma model has not been fully investigated. The study aimed to examine the impact of PRF on skin wound healing in the cat trauma model. In this study, PRF from cats was successfully produced for our investigation. The models of feline trauma were effectively established. A total of 18 cats were randomly divided into 3 groups (n = 6): (1) Control group (CON); (2) PRF group; (3) Manuka honey group (MAN, as a positive control). Experiments were performed separately on days 7, 14, 21, and 28. Our results showed that PRF was a safe and efficient method of wound healing that did not influence the cat’s body temperature, respiration rate, and heart rate (HR). PRF accelerated skin wound healing in the cat trauma model based on the rate and histological observation of wound healing. In addition, PRF promoted the production of growth factors and suppressed inflammation during wound healing. PRF accelerated wound healing by increasing the formation of collagen fibers, as shown by Masson-trichrome staining. The outcomes of the PRF and MAN groups were comparable. In conclusion, PRF improves the healing of skin wounds in cats by boosting the synthesis of growth factors, reducing inflammation, and enhancing the synthesis of collagen fibers.Graphical

Current Trends on Innovative Technologies in Topical Wound Care for Advanced Healing and Management.

To investigate critically traditional and modern techniques for cutaneous wound healing and to provide comprehensive information on these novel techniques to encounter the challenges with the existing wound healing methods. The financial burden and mortality associated with wounds is increasing, so managing wounds is essential. Traditional wound treatments include surgical and non-surgical methods, while modern techniques are advancing rapidly. This review examines the various traditional and modern techniques used for cutaneous wound healing. Traditional wound treatments include surgical techniques such as debridement, skin flaps, and grafts. Non-surgical treatments include skin replacements, topical formulations, scaffold-based skin grafts, and hydrogel-based skin dressings. More modern techniques include using nanoparticles, growth factors, and bioactive substances in wound dressings. Bioengineered skin substitutes using biomaterials, cells, and growth factors are also being developed. Other techniques include stem cell therapy, growth factor/cytokine therapy, vacuum-assisted wound closure, and 3D-printed/bio-printed wound dressings. Traditional wound treatments have been replaced by modern techniques such as stem cell therapy, growth factor/cytokine therapy, vacuum-assisted wound closure, and bioengineered skin substitutes. However, most of these strategies lack effectiveness and thorough evaluation. Therefore, further research is required to develop new techniques for cutaneous wound healing that are effective, cost-efficient, and appealing to patients.

Application of polymer adhesive hydrogel in wound healing

As the global adhesive industry continues to grow, researchers are discovering that polymer adhesives are playing an increasing role in the medical field, polymer adhesives have attracted more and more attention from the medical industry due to their unique advantages, such as hydrogels, have gradually appeared, which can effectively help wound healing. Most of the hydrogels have good biocompatibility and water absorption, and have the advantages of controllable structure, adjustable performance and easy degradation. Compared with traditional wound healing methods, hydrogels effectively reduce additional trauma and leakage and allow for greater tissue integration. This paper reviews the types of polymeric adhesive hydrogels and examples of some of them being artificially modified to be applied to the field of wound healing. Analyzes their mechanism of action and the advantages and shortcomings of their clinical applications, and finally looks into the potential functions and clinical efficacy of hydrogels in the future.

Harnessing Nature's Potential: Innovating Antibacterial Cream through Garlic and Clove Extracts

The onset of the antibiotics period was characterised by the clinical implementation of penicillin, the inaugural antibiotic, unearthed in 1928 by Alexander Fleming in the green mould Penicillium notatum. Garlic and clove have long been used as traditional treatments for different diseases, including infections. Their antibacterial qualities have been attributed to their sulfur-containing components, such as allicin and ajoene in garlic, and eugenol and isoeugenol in clove. In this study, we wanted to produce a novel antibacterial cream leveraging the synergistic properties of garlic and clove extracts. The cream was manufactured utilizing a simple and scalable technique, and its antimicrobial activity was assessed against a panel of microbes present in our surrounding. The results indicated that the cream had considerable antibacterial action against all tested microorganisms, with minimum inhibitory concentrations (MICs) comparable to conventional antibiotics. This study establishes a groundbreaking, ecologically-oriented method for safeguarding and healing the skin. The tropical antimicrobial cream provides a secure and efficient substitute for traditional antimicrobials that may have harsh effects, while also including the nourishing and rejuvenating qualities of tropical botanicals. This groundbreaking research marks a significant advancement in the realm of natural, holistic skin health solutions. The tropical antimicrobial cream stands as a potent alternative to conventional, potentially harsh antimicrobials, offering a safe and effective way to combat skin infections and irritations. Furthermore, its incorporation of nourishing and restorative tropical botanicals transcends mere protection, promoting overall skin health and well-being. Beyond the individual benefits, this innovation holds immense potential for promoting sustainable practices within tropical ecosystems by leveraging resources responsibly and ethically. This paves the way for a future where nature's wisdom empowers us to safeguard our skin with gentle effectiveness, while simultaneously cherishing and preserving the biodiversity that holds the key to our well-being.

The value of assessing deep disease healing by probe-based confocal laser endomicroscopy and histology for long-term prognosis of ulcerative colitis.

The benefits of deep disease healing need evaluation by long-term clinical research in different populations. Confocal laser endomicroscopy (CLE) is a superior method for evaluating deep disease healing. This prospective study enrolled ulcerative colitis (UC) patients in clinical remission who underwent colonoscopy, CLE, and histological assessment. Patients were monitored for relapse by patient-reported outcomes and colonoscopy evaluation of mucosal healing. The ability of different methods of mucosal healing to predict long-term disease recurrence was assessed using Kaplan-Meier estimation and Cox proportional hazard regression. Forty-two patients in clinical remission were assessed by colonoscopy. Those with Mayo endoscopic subscores (MES)≤1 were enrolled. The 48-month recurrence rates in present healing group, assessed by CLE (colonic barrier assessment and ENHANCE index) and by histological examination (Geboes scale), were 20.0%, 26.7%, and 11.1%, respectively, and were significantly lower than absent healing group (P<0.05). Univariate Cox proportional risk regression analysis in absent of healing disease, determined by the ENHANCE index and Geboes scale, indicated an increased risk of recurrent events, with hazard ratios (HR) of 3.87 (95% CI: 1.18, 12.62) and 8.20 (95% CI: 1.06, 63.30), respectively. Multivariate Cox proportional hazard regression analysis adjusted for the extent of inflammation (E3 or not) showed a significant difference only for the ENHANCE index, with an HR of 3.53 (95% CI: 1.03, 12.10), P=0.045. Deep disease healing has a lower recurrence rate. The colonic barrier healing assessment, ENHANCE index, and histological Geboes scale have superior long-term prognostic value for UC patients.

Aurelia aurita jellyfish collagen: Recovery properties

Wound and burn healing is a complex physiological process that can be facilitated by medications based on marine collagen. In this regard, biomass of the Aurelia aurita jellyfish is a promising alternative source of medical collagen. As the global incidence of burns and wounds continues to grow, new healing methods have become a relevant area of medical science. This study featured acetic acid as a means of marine collagen extraction from A. aurita biomass. The physical and chemical properties of jellyfish collagen were determined gravimetrically and included such indicators as water solubility and water holding capacity. The molecular weight was defined by gel electrophoresis. The spectral studies relied on the method of UV spectroscopy. The regenerative experiments included such parameters as cytotoxicity, antioxidant properties, adhesion, and wound healing rate, as well as a quantitative PCR analysis. The optimal conditions for maximal collagen yield were as follows: 0.5 M acetic acid and 48 h extraction time. However, the collagen yield was very low (≤ 0.0185%). The high water holding capacity showed good prospects for A. aurita collagen to be used as hemostatic sponge. The acid-soluble collagen sample had a molecular weight of 100–115 kDa, which made it possible to classify it as type I. A. aurita jellyfish collagen revealed no cytotoxic properties; it had no effect on adhesion, migration, and proliferation of keratinocytes, neither did it affect the expression of cell differentiation markers. The wound healing model proved that the marine collagen had regenerative properties as it was able to increase the wound healing rate by 24.5%. Therefore, collagen extracted from the biomass of A. aurita jellyfish d emonstrated good p rospects for cosmetology and regenerative medicine.

Evaluation of Çankırı and Iğdır salt caves within the scope of health and recreation tourism

With the increasing popularity of healing methods involving salt, the importance of salt mines has grown significantly over time. Today, as salt therapy (Speleotherapy) becomes more widespread, salt caves in many countries around the world have begun to transform into salt therapy centers. Salt caves in Spain (Cardona), Belarus (Soliharsk), Romania (Slanic, Salina Turda, and Praid), Poland (Wieliczka), Pakistan (Khewra), Germany (Berchtesgaden), Azerbaijan (Nakhchivan), and Turkey (Çankırı and Iğdır Tuzluca) are used for therapeutic purposes. Particularly, Çankırı Salt Cave and Iğdır Tuzluca Salt Cave are important resources in Turkey that can be evaluated within the scope of health tourism. In this study, a literature review, official website analysis, and on-site observations were conducted. In the literature review, previous research on the subject was examined. Through website analysis, the spatial units and recreational activities of the salt caves were identified. The spatial units and recreational activities of each salt cave were determined using the collected data. Subsequently, on-site observations were made by visiting the salt caves in Çankırı and Iğdır. During these observations, the spatial units within the salt caves were visited and photographed individually. Then, the data obtained from the literature, official website analyses, and on-site observations were combined. Finally, the characteristics of all the examined caves were tabulated, and based on the created table, the caves were evaluated. As a result of the evaluation, the Cardona Salt Mine in Spain stands out for its historical and cultural activities; the Nakhchivan in Azerbaijan, Soligorsk in Belarus, Slanic and Praid Salt Mines in Romania are treatment centers offering salt therapy services; the Salina Turda Salt Mine in Romania is an underground theme park; the Wieliczka Salt Mine in Poland, Khewra Salt Mine in Pakistan, and Berchtesgaden Salt Mine in Germany are primarily used for recreational purposes; while the developing Çankırı and Iğdır Tuzluca Salt Mines in Turkey are also noted for their recreational use.

Estimation of the biaxial tensile behavior of ovine esophageal tissue using artificial neural networks

Diseases of the esophagus affect its function and often lead to replacement of long sections of the organ. Current healing methods involve the use of bioscaffolds processed from other animal models. Although the properties of these animal models are not exactly the same as those of the human esophagus, they nevertheless present a reasonable means of assessing the biomechanical properties of the esophageal tissue. Besides, sheep bear many similarities physiologically to humans and they also suffer from same diseases as humans. The morphology of their esophagus is also comparable to that of humans. Thus, in the study, an ovine esophagus was studied. Studies on the planar biaxial tests of the gross esophageal anatomy are limited. The composite nature of the gross anatomy of the esophagus makes the application of structure-based models such as Holzapfel-type models very difficult. In current studies the tissue is therefore often separated into specific layers with substantial collagen content. The effects of adipose tissue and other non-collagenous tissue often make the mechanical behavior of the esophagus widely diverse and unpredictable using deterministic structure-based models. Thus, it may be very difficult to predict its mechanical behavior. In the study, an NARX neural network was used to predict the stress–strain response of the gross anatomy of the ovine esophagus. The results show that the NARX model was able to achieve a correlation above 99.9% within a fitting error margin of 16%. Therefore, the use of artificial neural networks may provide a more accurate way of predicting the biaxial stress–strain response of the esophageal tissue, and lead to further improvements in the design and development of synthetic replacement materials for esophageal tissue.

Skin organoid transplantation promotes tissue repair with scarless in frostbite.

Frostbite is the most common cold injury and is caused by both immediate cold-induced cell death and the gradual development of localized inflammation and tissue ischemia. Delayed healing of frostbite often leads to scar formation, which not only causes psychological distress but also tends to result in the development of secondary malignant tumors. Therefore, a rapid healing method for frostbite wounds is urgently needed. Herein, we used a mouse skin model of frostbite injury to evaluate the recovery process after frostbite. Moreover, single-cell transcriptomics was used to determine the patterns of changes in monocytes, macrophages, epidermal cells and fibroblasts during frostbite. Most importantly, human-induced pluripotent stem cell (hiPSC) -derived skin organoids combining with gelatin-hydrogel were constructed for the treatment of frostbite. The results showed that skin organoid treatment significantly accelerated wound healing by reducing early inflammation after frostbite and increasing the proportions of epidermal stem cells. Moreover, in the later stage of wound healing, skin organoids reduced the overall proportions of fibroblasts, significantly reduced fibroblast-to-myofibroblast transition by regulating the integrin α5β1-FAK pathway, and remodeled the extracellular matrix (ECM) through degradation and reassembly mechanisms, facilitating the restoration of physiological ECM and reducing the abundance of ECM associated with abnormal scar formation. These results highlight the potential application of organoids for promoting the reversal of frostbite-related injury and the recovery of skin functions. This study provides a new therapeutic alternative for patients suffering from disfigurement and skin dysfunction caused by frostbite.

Columbianadin ameliorates rheumatoid arthritis by attenuating synoviocyte hyperplasia through targeted vimentin to inhibit the VAV2/Rac-1 signaling pathway

IntroductionRheumatoid arthritis (RA) is an autoimmune disease pathologically characterized by synovial inflammation. The abnormal activation of synoviocytes seems to accompany the progression of RA. The role and exact molecular mechanism in RA of columbianadin (CBN) which is a natural coumarin is still unclear. ObjectivesThe present research aimed to investigate the effect of vimentin on the abnormal growth characteristics of RA synoviocytes and the targeted regulatory role of CBN. MethodsCell migration and invasion were detected using the wound healing and transwell method. Mechanistically, the direct molecular targets of CBN were screened and identified by activity-based protein profiling. The expression of relevant proteins and mRNA in cells and mouse synovium was detected by western blotting and qRT-PCR. Changes in the degree of paw swelling and body weight of mice were recorded. H&E staining, toluidine blue staining, and micro-CT were used to visualize the degree of pathological damage in the ankle joints of mice. Small interfering RNA and plasmid overexpression of vimentin were used to observe their effects on MH7A cell proliferation, migration, apoptosis, and downstream molecular signaling. ResultsThe TNF-α-induced proliferation and migration of MH7A cells could be significantly repressed by CBN (25,50 μM), and the expression of apoptosis and autophagy-associated proteins could be modulated. Furthermore, CBN could directly bind to vimentin and inhibit its expression and function in synoviocytes, thereby ameliorating foot and paw swelling and joint damage in CIA mice. Silencing and overexpression of vimentin might be involved in developing RA synovial hyperplasia and invasive cartilage by activating VAV2 phosphorylation-mediated expression of Rac-1, which affects abnormal growth characteristics, such as synoviocyte invasion and migration. ConclusionCBN-targeted vimentin restrains the overactivation of RA synoviocytes thereby delaying the pathological process in CIA mice, which provides valuable targets and insights for understanding the pathological mechanisms of RA synovial hyperplasia.

Review on Evaluation Methods of Fracture Healing

Review on Evaluation Methods of Fracture Healing

Care-seeking pathways and treatment practices for snakebite in Odisha, India

PurposeSnakebites represent a pressing global public health concern, with India shouldering a disproportionate burden and often dubbed the "snakebite capital." Traditional and spiritual healing methods prevail in India, yet their efficacy and potential adverse health consequences warrant investigation. This study seeks to elucidate the perspectives of healthcare providers and caretakers regarding snakebite treatment practices, with a specific emphasis on traditional healing modalities.MethodsAn exploratory study was conducted among ten healthcare providers–five traditional and five modern treatment providers, two snake trappers/catchers and 120 snakebite victims or their caretakers across three districts of Odisha, India. Data were collected through in-depth interviews and analysed using content analysis.ResultsThe analysis revealed five main categories: various snakebite treatment practices, care-seeking pathways of victims, perspectives of patients and caretakers on treatment, traditional healers’ views on modern treatment, and allopathic doctors’ views on traditional treatment. Treatment practices observed included allopathic treatment, cold treatment, spiritual healing practices at temples, brass plate treatment, mantra enchanting, herbal treatment, and self-medication. Reasons for treatment delay included strong community beliefs in traditional healing, lack of control over irrational treatment by traditional healers, suboptimal care at primary and secondary healthcare facilities, and poor community literacy on snakebites.ConclusionsThe study emphasizes the need for improved snakebite preparedness strategies, community literacy, active involvement in prevention programs, and strong political commitment to control irrational treatment practices.

Utilizing Indigenous Flora in East Africa for Breast Cancer Treatment: An Overview

Background: Breast cancer is a significant global health challenge, contributing substantially to cancer- related deaths. Conventional treatment methods, including hormone therapy, chemotherapy, surgical interventions, and radiation, have long been utilized. However, these traditional treatments are often associated with serious side effects and drug resistance, limiting their efficacy. Aim: This review aims to explore the potential of medicinal plants used in breast cancer management in East Africa, focusing on their bioactive compounds and anticancer properties. Methods: A comprehensive literature search was conducted to examine the effectiveness of medicinal plants in treating breast cancer across Kenya, Ethiopia, Uganda, Tanzania, and Rwanda. Relevant studies published between 2003 and 2023 were identified using keywords related to breast cancer and medicinal plants. The search was performed across multiple databases, including Google Scholar, PubMed, Scopus, Web of Science Core Collection, and Science Direct. Results: Numerous natural compounds found in East African medicinal plants including Cymbopogon citratus (Lemongrass,) Tabebuia avellanedae, Prunus africana (African Cherry), Euclea divinorum, Berberis holstii, Withania somnifera (Ashwagandha, Curcuma longa (Turmeric), Garcinia mangostana (Mangosteen, Vitis vinifera (Grapevine), Eugenia jambolana (Java Plum), Moringa oleifera (Drumstick Tree), Camellia sinensis (Tea), Glycine max (Soybean), Catharanthus roseus, Madagascar Periwinkle), Rhus vulgaris (Wild Currant) exhibit significant anticancer properties. These compounds have demonstrated the ability to reduce breast cancer aggressiveness, inhibit cancer cell proliferation, and modulate cancer-related pathways. Current research focuses on these natural and dietary compounds to develop more effective strategies for treating breast cancer. Conclusion: The findings suggested that East African medicinal plants hold promise as complementary treatments for breast cancer, offering potential benefits such as affordability, cultural appropriateness, and sustainability. Further research into these plants and their bioactive compounds could revolutionize breast cancer treatment, improving survival rates and addressing the rising incidence of breast cancer-related fatalities. Other: The review underscores the importance of continued research, conservation, and the integration of ancient healing methods to fully harness the potential of East African flora in breast cancer management.

Synergistic integration of electrical stimulation, reactive oxygen species regulation, and pro-angiogenic for accelerated wound healing

Wound healing involves a complex series of coordinated events throughout the inflammatory, proliferative, and maturation phases of tissue repair. Current treatment modalities lack a device catering to all wound healing stages for tissue recovery, angiogenesis and epithelialization. Herein, we developed an integrated wound healing system with multiple functions of self-electrical stimulation (ES), reactive oxygen species (ROS) regulation, and bioactive metal-releasing patch (ERMP) to address all wound healing stages including hemostasis, inflammation, proliferation, and remodeling. The multiple functions of ERMP are ES, generated by the movement of the mice, for tissue regeneration, magnesium (Mg) ion release via iontophoresis triggered by ES for pro-angiogenic effects, and simultaneous regulation of ROS. This system employs self-generating ES utilizing the movements of the individuals to trigger triboelectric nanogenerators (TENGs), which consequently transfer ES through Mg microneedles while effectively delivering enhanced Mg ions with an iontophoresis via ES to the wound site. Specially, Prussian blue (PB) was utilized to not only enhance output performance on TENG as charge trap, but also regulate excessive ROS via modified PB with carbon–nitrogen vacancies in the wound site. The synergistic effects of ES, ROS regulation, and enhanced Mg ions releases led to a nine-fold recovery rates in wound healing compared to the control group in vivo tests. This approach addresses the inherent limitations of conventional wound healing methods and provides a viable treatment for various severe wound types.

Network Practice of Rare Disease Bloggers and Life Narrative: Take ALS as an Example

As a healing method, narrative is widely concerned by the medical community. The characteristics of "Hyper-temporal", "sharing" and "personalized" of new media provide convenient conditions for the self-performance of rare disease groups and also encourage them to re-choose narrative content and strategies. This paper adopts in-depth interview and text analysis to analyze the network practice and narrative behavior of Amyotrophic lateral sclerosis (ALS) web celebrities. The study found that the online narrative of ALS is not only the life practice of bloggers' self-healing, self-persuasion and self-empowerment, but also their positive response to real family and social responsibilities. At the same time, the online celebrity career of rare disease bloggers reflects their life courses in a certain sense. The social media accounts of rare disease bloggers are an important carrier for them to enrich themselves and place their feelings and expectations, and the continuation of their digital life has brought new philosophical and ethical thinking.

The effect of adipose tissue-derived stromal vascular fraction/gel material on wound healing in a rat model of nasal mucosa injury

Background Stromal Vascular fraction/gel (SVF/gel) is prepared mechanically from autologous adipose tissue, and it is known for its regenerative and anti-inflammatory properties. Aims To assess histopathological effects of adipose tissue-derived SVF/gel and nasal steroids on nasal mucosal healing Material and Methods Forty-two Wistar Albino rats with right nasal mucosal injury were randomly divided into three groups: control (saline), Mometasone Furoate (MF), and SVF/gel. Control group (n = 14) received saline for 7 days, while MF group (n = 14) was administered MF to the right nasal cavity for 7 days. SVF/gel group (n = 14) was treated once with SVF/gel in the right nasal cavity. Histological analysis on days 14 and 28 post-injury focused on evaluating epithelial thickness, inflammation, disarray, subepithelial thickness, goblet cell count, subepithelial fibrosis, presence of ciliated cells, lacunae, adhesion, and neo-osteogenesis. Results When comparing the MF and SVF/gel groups, statistically significant differences were found on day 14 in indices of epithelial thickness, subepithelial thickness, goblet cells, subepithelial fibrosis, and ciliated cells. On day 28, SVF/gel group exhibited higher ciliated cell counts and lower subepithelial fibrosis values (p = .027; p = .016). Additionally, epithelial disarray, adhesions, lacunae, and neo-osteogenesis were not observed in the SVF/gel group. Conclusions and Significance SVF/gel accelerates re-epithelialization, reduces fibrosis and adhesions, and enhances cilia formation compared to nasal steroids. These findings suggest that SVF/gel is an autologous and cost-effective treatment for improving nasal mucosal healing post-injury.

Rapid Recovery of Degraded Perovskite Single-Crystal Radiation Detectors via Infrared Healing.

Radiation detectors based on metal halide perovskite (MHP) single crystals (SCs) have exhibited exceptional sensitivity, low detection limit, and remarkable energy resolution. However, the operational stability issue still dramatically impedes their commercialization due to degradation induced by high-energy irradiation and large bias. Here, we propose an innovative infrared healing strategy to restore the devices that have undergone severe damage from both long-term biasing and X-ray irradiation. Compared to the slow and inefficient intrinsic self-healing process of MHPs, the infrared healing method demonstrates the capacity to achieve rapid recovery of the detection performance of the degraded devices within just 1 h. We reveal that the healing mechanism is mainly related to the reduction of the ion-migration activation energy in MHP SCs under infrared illumination, which promotes the back diffusion of the displaced ions to their original lattice positions and remedies defects. Finally, the healing effect is further confirmed through the gamma-ray spectroscopy acquisition with degraded MHP SCs, whose energy resolution at 59.5 keV of 241Am source is improved from 36% to 12% following infrared illumination. These results present infrared healing as a simple and economic method to extend the service life of MHP SC-based detectors.

Tetrahydroisoquinoline reduces angiogenesis by interacting myeloma cells with HUVECs mediated by extracellular vesicles.

Multiple myeloma (MM) is a neoplastic condition resulting from the uncontrolled expansion of B-cell-derived plasma cells. The importance of angiogenesis in MM development has also been demonstrated. Extracellular vesicles (EVs) have vital functions in interactions between neighboring cells, such as angiogenesis. The objective of this in vitro study was to examine the transfection and angiogenesis effects of MM-EVs on endothelial cells (ECs) upon treatment with Tetrahydroisoquinoline (THIQ) as a bioactive organic compound derivative from isoquinoline. Following treatment of multiple myeloma cells (U266) with THIQ, MM-EVs were harvested and transmigrated to human umbilical vein endothelial cells (HUVEC) in a co-culture model. EVs transmigration was traced by flow cytometry. Correspondingly, the expression of angiogenic genes and/or proteins in U266 cells and HUVECs was measured by RT-PCR and ELISA methods. Likewise, the proliferation and migration of HUVECs treated with THIQ-treated MM-EVs were visualized and estimated by performing both tube formation and scratch wound healing methods. Surprisingly, the anti-angiogenic effect of THIQ-treated MM-EVs was evident by the decreased expression of CD34, VEGFR2, and IL-6 at the mRNA and/or protein levels after internalization of MM-EVs in HUVEC. Finally, tube formation and scratch wound healing experiments showed inhibition of HUVEC cell proliferation and migration by THIQ-treated MM-EVs compared to control MM-EVs. MM-EVs derived from THIQ-treated myeloma cells (U266) inhibited angiogenesis in HUVECs. This phenomenon is coordinated by the internalized THIQ-treated MM-EVs in HUVECs, and ultimately the reduction of angiogenic factors and inhibition of tube formation and scratch wound healing.

MIGRATION INHIBITION ACTIVITY BY METHANOL EXTRACT Hibiscus tiliaceus Linn. ON 4T1 BREAST CANCER

The prevalence of breast cancer cases in Indonesia is increasing along with the ability of cancer cells to migrate or move from the primary tumor mass and form new colonies elsewhere. The migration of cancer cells has encouraged the develop anticancer drugs from natural ingredients. Waru leaves have been shown to have cytotoxic activity. This study aims to determine the inhibition activity of migration of breast cancer cells 4T1 from methanol extract of waru leaves. Waru leaves methanol extract was obtained using the maceration method. Cytotoxic test of methanol extract of waru leaves (MEWL) was the migration test used in the scratch wound healing method at concentrations 162.5, 325, and 650 μg/mL at 0, 18, 24, and 42 hours after treatment. Analysis of IC50 using linear regression, while large areas were analyzed using Image-J software. The percentage of data closure was analyzed statistically with the Anova Repeated Measure test. All concentrations of Methanol Extract of Waru Leaves had significant inhibition of cell migration (p&lt;0,05) compared to control at each observation time at 0, 18, 24, and 42 hours after treatment. So, MEWL is able to inhibit migration in 4T1 cells

Assessment of Plants Used in Polyherbal Formulations for Traditional Treatment of Skin Infections in Ibadan Metropolis

IntroductionThere are numerous skin diseases and frequently occurring health problems that affect all ages, from neonates to the elderly, and in many ways cause harm. Natural treatment is cheap and claimed to be safe. It is also suitable for raw materials or the production of new synthetic drugs.The present study was aimed at carrying out an ethnobotanical investigation of polyherbal formulations traditionally used for the treatment of skin infections in the Ibadan metropolis. MethodsA comprehensive ethnobotanical survey was carried out in six local government areas within the Ibadan metropolis of Oyo State, Nigeria, where a substantial number of respondents were administered questionnaires. Information on the respondents' socioeconomic status, the types of plants utilised in polyherbal medicine, and the preparation and skill-acquisition techniques of the local herbal practitioners were all gathered. The generated data were subjected to descriptive statistics analysis. ResultsThe obtained results showed that Daniella oliveri, Olax subcorpioidea, Khaya grandifolia, Piper guineensis, Anogesius leocarpa, and Alstonia boonei are the most often utilised herbs for treating skin infections. Barks are mainly used because the highest occurring mode of preparation recorded was decoction, and barks are mostly decocted to release the active constituents in them for the treatment of fungal skin infections. The most common method of administration of the herbal preparations was drinking the decoction after preparation. ConclusionsThis study confirmed that polyherbal medicines have great potential in treating various types of skin infections and a few rural and urban dwellers still depend on traditional healing methods.