Healing Effect Research Articles

Evaluating the therapeutic impact of Compound Polymyxin B Ointment on postoperative wound healing in patients with perianal abscesses

IntroductionPerianal abscesses pose a considerable obstacle in the realm of postoperative wound treatment owing to their elevated susceptibility to infection and associated consequences. Polymyxin B Ointment, a compound renowned for its antibacterial qualities, has the potential to provide therapeutic advantages by promoting wound healing and mitigating postoperative problems.MethodsOur institution conducted a thorough retrospective analysis spanning from December 2020 to December 2023 to assess the effectiveness of Compound Polymyxin B Ointment in the management of surgical wounds in patients diagnosed with perianal abscesses. The research encompassed a cohort of 100 individuals, who were classified into two groups: a control group that received conventional postoperative care, and an observation group that received supplementary treatment with Compound Polymyxin B Ointment. The evaluation of clinical outcomes involved measuring wound healing effectiveness, pain intensity using the Visual Analogue Scale (VAS), tissue swelling, exudation, necrotic tissue shedding time, duration of hospital stays, and rate of reduction in wound area.ResultsThe group that received Compound Polymyxin B Ointment had significant enhancements in wound healing, as seen by a noteworthy 46% of participants completing complete healing, in contrast to the control group’s 32%. The VAS was used to quantify pain levels, and the observation group reported a substantial reduction of almost 50% in ratings. Furthermore, this cohort exhibited a 45% decrease in edema and a 50% decline in exudation rates, in addition to a 50% acceleration in the shedding of necrotic tissue. The duration of the hospital stay was reduced by 40%, and the reduction in wound area was 18% higher, suggesting a more effective healing process. In addition, it is worth noting that the observation group had a lower incidence of problems, so underscoring the effectiveness of the ointment in facilitating wound healing and mitigating postoperative difficulties.DiscussionThe utilization of Compound Polymyxin B Ointment as an adjuvant measure in the surgical treatment of perianal abscesses has been found to have a substantial positive impact on wound healing, pain relief, and complication reduction. This finding provides evidence for the possibility of the ointment as a helpful inclusion in post-surgical wound care procedures among this specific group of patients.

Exosomes derived from minor salivary gland mesenchymal stem cells: a promising novel exosome exhibiting pro-angiogenic and wound healing effects similar to those of adipose-derived stem cell exosomes

BackgroundsMinor salivary gland mesenchymal stem cells (MSGMSCs) can be easily extracted and have a broad range of sources. Applying exosomes to wounds is a highly promising method for promoting wound healing. Exosomes derived from different stem cell types have been proven to enhance wound healing, with adipose-derived stem cell (ADSC)-derived exosomes being the most extensively researched. Considering that MSGMSCs have advantages such as easier extraction compared to ADSCs, MSGMSCs should also be a very promising type of stem cell in exosome therapy. However, whether MSGMSC-derived exosomes (MSGMSC-exos) can promote wound healing and how they compare to ADSC-derived exosomes (ADSC-exos) in the wound healing process remain unclear.MaterialsThe effects of MSGMSC-exos and ADSC-exos on angiogenesis in wound healing were investigated in vitro using CCK-8, scratch assays, and tube formation assays. Subsequently, the promotion of wound healing by MSGMSC-exos and ADSC-exos was evaluated in vivo using a full-thickness wound defect model in mice. Immunohistochemistry was used to verify the effects of MSGMSC-exos and ADSC-exos on promoting collagen deposition, angiogenesis, and cell proliferation in the wound. Immunofluorescence staining was performed to investigate the role of MSGMSC-exos and ADSC-exos in modulating the inflammatory response in the wound. Furthermore, proteomic sequencing was conducted to investigate the functional similarities and differences between the proteomes of MSGMSC-exos and ADSC-exos, with key protein contents verified by ELISA.ResultsMSGMSC-exos exhibited similar effects as ADSC-exos in promoting the migration, proliferation, and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro, with a comparable dose-dependent effect. In vivo experiments confirmed that MSGMSC-exos have similar wound healing-promoting functions as ADSC-exos. MSGMSC-exos promoted the neovascularization and maturation of blood vessels in vivo at a level comparable to ADSC-exos. Despite MSGMSC-exos showing less collagen deposition than ADSC-exos, they exhibited stronger anti-scar formation and anti-inflammatory effects. Proteomic analysis revealed that the proteins promoting wound healing in both MSGMSC-exos and ADSC-exos were relatively conserved, with ITGB1 identified as a critical protein for angiogenesis. Further differential analysis revealed that the functions specifically enriched in MSGMSC-exos and ADSC-exos reflected the functions of their source tissue.ConclusionsOur study confirms that MSGMSC-exos exhibit highly similar wound healing and angiogenesis-promoting functions compared to ADSC-exos, and the proteins involved in promoting wound healing in both are relatively conserved. Moreover, MSGMSC-exos show stronger anti-scar formation and anti-inflammatory effects than ADSC-exos. This suggests that MSGMSCs are a promising stem cell source with broad applications in wound healing treatment.

Therapeutic Potential of the Herbal Plant Uncaria gambir: A Systematic Review

Background: Uncaria gambir is an Indonesian medicinal herb with healing effects. This plant contains flavonoids, which can be used as antioxidants and anti-inflammatory agents to accelerate healing. In Indonesia, Uncaria gambir is widely accessible, simple to grow, and has a long history of use as an alternative medicine. Following this, studies are conducted in the medical industry using Uncaria gambir. Objective: This study aimed to comprehensively review the use of Uncaria gambir extract as an herbal plant for treating experimental animals. Methods: This review followed the Preferred Reporting Items for Systematic Reviews and Meta- Analyses guidelines, and the risk of bias was evaluated by modifying the ARRIVE guidelines. Results: A report was presented on review articles from 12 publications sourced from PubMed, ScienceDirect, Wiley Online Library, ProQuest, EBSCO Host, and other relevant studies. The Uncaria gambir isolates tested were catechin and ethyl acetate, used primarily for lesions, pain, edema, and diabetes in experimental Wistar rats. Conclusion: Uncaria gambir is an herbal plant that contains flavonoids, which can be used as antiinflammatory, antioxidant, antibacterial, and hepatoprotective agents and can potentially be applied in the medical field as adjuvant therapy.

ZnCur nanoparticle-enhanced multifunctional hydrogel platform: Synergistic antibacterial and immunoregulatory effects for infected diabetic wound healing

ZnCur nanoparticle-enhanced multifunctional hydrogel platform: Synergistic antibacterial and immunoregulatory effects for infected diabetic wound healing

Bacoside A: A Promising Medication for Treatment of Various Disorders

Abstract: Bacoside A is a triterpenoid saponin and nootropic herb that can be extracted from Bacopa monnieri Linn., Scrophulariaceae. It is a glabrous and succulent herb used traditionally for centuries in treating various illnesses, mainly as a cardiotonic and nerve tonic, and has been considered an eminent Ayurvedic medicine. Various studies reported that the herb consists of a wide range of potentially bioactive, phytochemical constituents with synergistic effects. Bacoside A is a major constituent of B. monnieri, with diverse biological functions and significant therapeutic potential, as given by various researchers and preclinical studies. It possesses various biological activities such as hepatoprotective, anti-osteoporotic, anti-ulcerogenic, anti-inflammatory, anti-tumor, wound healing, and neuroprotective effects. This review compiled the preclinical evidence-based studies and present knowledge of bacoside A's potential benefits and fruitful impact on human health, and we provided a comprehensive perspective of bacoside A in various disease treatments.

A Comprehensive Review on Niosomes as a Strategy in Targeted Drug Delivery: Pharmaceutical, and Herbal Cosmetic Applications.

Niosomes are newly developed, self-assembling sac-like transporters that deliver medication at a specific site in a focused manner, increasing availability in the body and prolonging healing effects. Niosome discovery has increased drugs' therapeutic effectiveness while also reducing adverse effects. This article aims to concentrate on the increase in the worldwide utilization of niosomal formulation. This overview presents a thorough perspective of niosomal investigation up until now, encompassing categories and production techniques, their significance in pharmaceutical transportation, and cosmetic use. The thorough literature review revealed that extensive attention has been given to developing nanocarriers for drug delivery as they hold immense endeavor to attain targeted delivery to the affected area simultaneously shielding the adjacent healthy tissue. Many reviews and research papers have been published that demonstrate the interest of scientists in niosomes. Phytoconstituents, which possess antioxidant, antibiotic, anti-inflammatory, wound healing, anti-acne, and skin whitening properties, are also encapsulated into niosome. Their flexibility allows for the incorporation of various therapeutic agents, including small molecules, proteins, and peptides making them adaptable for different types of drugs. Niosomes can be modified with ligands, enhancing their targeting capabilities. A flexible drug delivery mechanism provided by non-ionic vesicles, which are self-assembling vesicular nano-carriers created from hydrating non-ionic surfactant, cholesterol, or amphiphilic compounds along comprehensive applications such as transdermal and brain-targeted delivery.

Invivo and ex-vivo evaluation and characterization of wound healing effect of topical calendulaofficinalis and turmeric cream in diabetic rats.

Objective: To evaluate wound healing by topically administration of calendulaofficinalis and tumeric cream to diabetic rats. Study Design: Observational Cross-sectional study Setting: Department of Pharmaceutics, Faculty of Pharmacy, BahaUddin Zakariya University, Multan, Pakistan Period: October 2020-march 2021. Method: Different batches of hydrophilic cream bases (C1-C6) were developed and characterized. The optimized base composition (C6) was loaded with calendula Officinal is extract and turmeric powder as an active ingredient (C7) and evaluated the rate at which wounds heal in diabetic rats. Calendula officinal is and turmeric were combined in a cream, as different stability parameters of the creams were analysed at different temperature. Microbiological action of the cream was also examined. Results: The formulation was homogenous, stable with pH 6.29±0.03, viscosity 381± 6.96 Cps and the microbiological quality met the requirements of European pharmacopeia. The total flavonoid content was less than 1. The formulation showed accelerated wound healing with more than 95% wound contraction after tenth day. Conclusion: The result were favorable and suggested that topical administration of Calendula officinal is and turmeric powder combination would enhance wound healing in diabetic rats with potential for diabetic foot ulcer treatment. Moreover, the designed formulation poses good quality emulsion system stabilizing the active ingredient as well as appropriate release of medicinal agents.

Construction of acidic microenvironment by Cu-TCPP nanozyme composited deprotonatable polymeric nanofibers for efficient antibacterial activity

The issue of bacterial resistance caused by conventional antibiotics has emerged as a major challenge in clinical treatment. Nanozymes, based on catalysis and devoid of bacterial resistance, provide a novel approach to eradicating bacteria. However, the practical potential of these nanozymes is limited by their low and readily pH-affected catalytic activity. Herein, the enzyme activity was optimized starting from the synthesis of nanozyme and the selection of carboxylate-containing polymers for the matrix. Specifically, various copper-tetrakis (4-carboxyphenyl) porphyrin (Cu-TCPP) were initially synthesized employing three copper sources, and among them, Cu-TCPP derived from Cu2O with higher catalytic activity was preferred. Subsequently, Cu-TCPP composite fibrous membranes were further obtained by electrospinning from their blend solutions with different polymers with distinct deprotonation capabilities. Thus, an acidic microenvironment conducive to the exertion of enzyme activity was constructed. The fabricated composite nanofibrous membrane could dissociate hydrogen ions to construct a localized acidic microenvironment even under weakly alkaline conditions, thereby enhancing the enzyme activity and improving the antibacterial effect. Further in vivo experiments demonstrated its favorable antibacterial ability and accelerated wound healing effect. The enhancement strategy of enzyme activity in this study brings inspiration for the design of nanozyme composited wound dressings and holds significant potential for clinical antibacterial applications

Vesicular phospholipid gels: A new strategy to improve topical antimicrobial dermatotherapy

Therapeutically effective and biocompatible dermal formulations that can ensure localization of a high level of antimicrobial drug at the site of action for an appropriate duration, while at the same time providing intrinsic reepithelization properties, are of particular importance for the treatment of infected and injured skin. The current research aimed to explore the potentials of using vesicular phospholipid gels (VPGs), semisolid formulations consisting of tightly packed liposomes (100–––200 nm), as innovative local depot drug vehicles for advanced topical dermatotherapy. Ciprofloxacin hydrocholoride (CPX) was selected as a model hydrophilic antibacterial drug and was loaded into several VPGs, differing in their composition. Various CPX-loaded VPGs (CPX-VPGs) were evaluated in vitro for the rheological and physicochemical characteristics, drug release profile, stability under in vivo mimicked conditions and during storage, skin permeability, biocompatibility with the epidermal cells, antibacterial efficacy and wound healing assay, to determine the optimal CPX-VPG for topical dermatotherapy. Viscosity and bilayers fluidity of VPGs affected the release of CPX from CPX-VPGs and its skin localization, limiting CPX percutaneous absorption. All CPX-VPGs exhibited even a 2-fold increase in anti-biofilm activity against both Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate compared to the free drug, while showing no toxic effects on human keratinocytes in vitro. Based on the pronounced proliferative effects on keratinocytes, superior in vitro wound healing effect and drug localization on/inside the skin, CPX-VPGs containing chitosan and hydrogenated phospholipid proved to be the most promising for topical dermatotherapy. These findings, along with increased bioadhesiveness and the slow drug release, with CPX concentrations significantly above the minimum biofilm inhibitory concentrations for bacteria typical in infected wounds, would contribute not only to the improvement of the antimicrobial dermatotherapy, but also to reduction of the frequency of the drug administration.

The Healing Effects of Poetry Reading on Anxiety Disorders: A Study Design Proposal

The Healing Effects of Poetry Reading on Anxiety Disorders: A Study Design Proposal

Healing Effects of Saroglitazar Gel in Thermally Induced Burn in Rats

Healing Effects of Saroglitazar Gel in Thermally Induced Burn in Rats

Comparison of Tegoprazan and Lansoprazole in Patients With Erosive Esophagitis up to 4 Weeks: A Multi-Center, Randomized, Double-Blind, Active-Comparator Phase 4 Trial.

The aims of this study were to confirm the non-inferiority of tegoprazan to lansoprazole up to week 4 in patients with erosive esophagitis (EE) and to evaluate its effectiveness in rapid mucosal healing and symptom relief at week 2. In this multi-center, randomized, double-blind, active-comparator non-inferiority trial, 218 patients with endoscopically confirmed EE (Los Angeles Classification Grades A-D) were randomly allocated to either the tegoprazan (50 mg) or lansoprazole (30 mg) group. The primary endpoint was the cumulative proportion of patients with healed EE up to week 4, as confirmed through endoscopy. The proportion of patients with healed EE at week 2 was also evaluated. Furthermore, CYP2C19 genotypes, symptoms, safety, and tolerability were assessed. In the full-analysis set, 103 and 109 participants in the tegoprazan and lansoprazole groups, respectively, were analyzed. The cumulative healing rates up to week 4 were 95.2% (98/103) and 86.2% (94/109) (difference [95% confidence interval], 8.91 [1.22-16.59]; p < 0.0001 for non-inferiority and 0.0266 for superiority), while those at week 2 were 88.4% (91/103) and 82.6% (90/109) (5.78 [-3.66-15.22], p = 0.0005 for non-inferiority) for tegoprazan and lansoprazole, respectively. Tegoprazan showed consistent healing rates regardless of CYP2C19 genotypes. Tegoprazan was superior to lansoprazole in the treatment of EE up to 4 weeks. Further studies are necessary to confirm these findings and clarify the superiority of tegoprazan, especially in the treatment of severe EE. ClinicalTrials.gov identifier: NCT05267743.

Monoclonal antibodies against jellyfish collagen.

Collagens are abundant structural proteins found in both mammalian and marine species, and attractive biomaterials used in various fields. Jellyfish collagen-based products have become increasingly popular because of their clinically proven health benefits such as the effects of skin wound healing and immune stimulation. To develop detection tools for jellyfish collagen, we generated four monoclonal antibodies, MCOL1, 2, 3, and 4, by immunizing mice with moon jellyfish collagen. The nucleotide and amino acid sequences of the variable regions of the monoclonal antibodies were determined. The antibody-binding kinetics toward collagens from moon jellyfish were evaluated using a surface plasmon resonance (SPR) biosensor, and the binding specificity was evaluated in comparison with binding to collagens from edible jellyfish, fish scales, and pig and cow skins. MCOL1, 3, and 4 specifically bound to moon jellyfish collagen, whereas MCOL2 bound to both moon and edible jellyfish collagens. Considering the results showing that the SPR responses of MCOL2 binding were greater than those seen with the other antibodies, MCOL2 could recognize the common and repetitive sequences of the two jellyfish collagens. Therefore, this monoclonal antibody will be most applicable for detecting jellyfish collagen.

Effectiveness of wide local excision and secondary intention healing in Hidradenitis Suppurativa: a single-center study on quality of life and mental health outcomes.

Hidradenitis suppurativa (HS) is a chronic inflammatory skin condition that significantly impacts patients' quality of life and mental health. Effective management often involves both medical and surgical interventions. The aim of the study was to assess the effectiveness of wide local excision and secondary intention healing in improving quality of life and mental health in patients with moderate to severe HS. A single-center prospective study was conducted with 40 patients suffering from moderate to severe HS, refractory to prior treatments. Pre-surgical ultrasound mapping of lesions was performed using Ultra High-Frequency Ultrasound (UHFUS). Patients underwent wide surgical excision followed by secondary intention healing based on HS-TIME principles. Quality of life was measured using Skindex-16, and mental health was assessed using the Hospital Anxiety and Depression Scale (HADS), with subscales for anxiety (HADS-A) and depression (HADS-D). Assessments were conducted at baseline, 4 weeks post-surgery, and after complete wound healing. Statistical analyses included paired t-tests and multiple linear regression to determine factors influencing outcomes. The study included 14 males and 26 females with a mean age of 39 years. Significant improvements were observed in Skindex-16 scores (pre: 57.92, post: 16.03) and HADS scores (HADS-A: pre: 6.13, post: 2.63; HADS-D: pre: 5.50, post: 3.21), indicating reduced pain, discomfort, and psychological distress. Multivariate analysis revealed that improvements were associated with male sex, HS stage II, longer disease duration, and lower BMI. Wide local excision combined with secondary intention healing significantly improves quality of life and mental health in HS patients. The findings suggest that a comprehensive approach addressing both surgical and psychological aspects can enhance patient outcomes. Future research should focus on long-term benefits and the development of standardized postoperative care protocols.

Wound Healing Potential of Herbal Hydrogel Formulations of Cedrus brevifolia Extracts in Mice.

Wound healing stands as a paramount therapeutic pursuit, imposing significant challenges on healthcare, particularly for vulnerable populations. Cedrus brevifolia, a species endemic to Cyprus, thrives in the Tripylos region, commonly known as Cedar Valley, within the Paphos forest. Despite its endemism, this species exhibits negligible genetic divergence from its Mediterranean related species. This study aims to investigate the potential of C. brevifolia resin and bark extracts in promoting wound healing in a mouse model. Previous in vitro investigations have elucidated the antioxidant and anti-inflammatory potential of extracts and isolates derived from the title plant, warranting further exploration in an in vivo setting. This experimental design employed 40 male SKH-hr2 black and brown mice aged 2-4 months. Wounds measuring 1 cm2 were meticulously induced in the anesthetized mice and the potential healing effect of the herbal hydrogel formulations was evaluated. The healing potential of the C. brevifolia extracts was rigorously assessed through the daily application of gel formulations containing resin concentrations of 5% and 10% w/w, alongside sapwood and heartwood extracts at concentrations of 0.5% and 1% w/w. The evaluation of the treatments encompassed a multifaceted approach, incorporating clinical observations, skin biophysical parameter assessments utilizing an Antera 3D camera, and FT-IR spectroscopy, in addition to histopathological examination. The chemical compositions were also investigated through NMR and bio-guided isolation. The most prominent herbal hydrogel preparation proved to be the 10% resin, followed by the sapwood at 1%. The chemical analysis unveiled abietic acid, manool, and lariciresinol derivatives that potentially contributed to the observed results. Bridging the gap between in vitro observations and in vivo outcomes attempts to shed light on the potential therapeutic benefits of C. brevifolia hydrogels in wound care.

A IMPORTÂNCIA DE ATIVOS NATURAIS NAS FORMULAÇÕES DE SKINCARE

INTRODUCTION: This study investigates the effectiveness of natural products in skincare beyond their aesthetic function, exploring their therapeutic properties for skin care. The analysis focuses on active ingredients such as Aloe Vera and essential oils from the Amazon, highlighting their antioxidant, anti-inflammatory and healing benefits for individualized treatments. GENERAL OBJECTIVE: The objective of this study is to investigate the effectiveness of natural active ingredients in cosmetics such as skincare, exploring their benefits beyond aesthetics. METHODOLOGY: This study presents an integrative bibliographic review on skincare and natural products, analyzing articles from 2020 to 2024 in databases such as BVS, PubMed, Google Scholar and Web of Science. Five relevant articles were selected to develop a review framework, focusing on the potential of cosmetics with natural products in adjuvant treatments in skincare. RESULTS: This study reviewed cosmetic formulations with natural products, focusing on their pharmacodynamic and pharmacokinetic properties to enhance aesthetic and therapeutic benefits, especially in combating skin aging and treating acne. six articles were analyzed that highlighted active ingredients such as Aloe Vera and cocoa extract, proving their antioxidant, anti-inflammatory and healing effects. CONCLUSION: The conclusion highlights that a skin care routine with natural cosmetics, such as Centella Asiatica and Aloe Vera, can prevent premature aging and treat conditions such as acnes, due to their anti-inflammatory and antioxidant properties.

Wound Healing Herbal Formulation from Essential Oil of Lemongrass (Cymbopogon citratus)

Untreated wound may lead to the infection. In some countries, plants are used to cure wound traditionally. Lemongrass (Cymbopogon citratus) has various bioactive compounds, including citronellol, limonene and geraniol that perform some pharmacological activities. In this study, the essential oil of lemongrass was designed into ointment formulation since lemongrass has been reported to have a wound healing effect in mice. The wound healing ointment made from different concentration of the essential oil of Cymbopogon citratus (F1: EOCC 5%, F2: EOCC 10% and F3: EOCC 15%) using a water-removable base and the characteristic as well as the stability (texture, color, smell, homogeneity, and pH) after been stored for 14 days was also evaluated. The result showed that all formulation (F1, F2, and F3) of wound healing formulation revealed good texture, consistency, odor, color, homogeneity and compatible to the skin until 14 days of storage. However, F3 (EOCC 15%) has a strong lemongrass smell compare to the other formulations.

Virtual Healing: Application of Augmented Reality Games in Pet Loss Syndrome

Abstract: As pets gain increasing significance in family life, pet loss syndrome has emerged as a mental health concern that demands urgent attention. When their pets pass away, many pet owners physically and psychologically isolate themselves, which makes it challenging to process unpleasant feelings in a healthy way. The purpose of this study is to investigate and explore how augmented reality (AR) game design can help users cope with the psychological trauma and emotional challenges caused by pet deaths. This study utilized user research, prototype design, user testing, and comparative research to develop and evaluate the usability and healing effects of the AR game. The research results indicate that this AR game has unique advantages in interactivity, immersion, and personalized experience. It can effectively provide users with emotional support, death education, and life enlightenment, helping them deal with negative emotions and gradually accept the reality of losing their pets. This study not only provides a new perspective for the treatment of pet loss syndrome, but also opens up new directions for the application of virtual therapy in the field of mental health, emphasizing the potential of electronic therapy games in emotional healing.

Evaluation of Platonia insignis Mart. (Bacuri Butter) and Biopolymers from the Puree of Allium cepa L. (Yellow Onion Bulb) for Wound Healing in Horses.

Background/Objectives: Skin injuries are common in the equine clinical practice, requiring effective treatment to support natural healing. Bacuri butter is gaining attention for its potential in wound healing and its anti-inflammatory, antimicrobial, and antioxidant properties. Natural polymers such as onion (Allium cepa) bioplastics have been investigated for their potential as occlusive dressings and for tissue regeneration. Methods: This study evaluated the healing process of experimentally induced skin wounds on horses treated with bacuri butter, washed onion film, and unwashed onion film. Clinical and histopathological analyses of the wounds were conducted in six clinically healthy horses over 28 days, with a control group receiving Ringer's lactate solution. The onion films were produced and characterized for their chemical structure and properties, while the bacuri butter was sourced and prepared for application. Results: All treatments, including the control group, promoted wound healing without relevant differences in wound contraction rates, gross aspect, or histopathological parameters. Conclusions: Therefore, despite minor variations observed in the clinical evaluations between the treatment groups, the bacuri butter or onion biopolymer showed no significant healing effect on skin wounds in horses. Additionally, this study showed the potential of equine models in testing novel therapeutic approaches for wound healing, benefiting both veterinary and human medicine.

Enhancing Skin Wound Healing in Diabetic Mice Using SIKVAV-Modified Chitosan Hydrogels.

Diabetic foot ulcers (DFUs), a prevalent chronic disease caused by various factors, significantly impact patients' quality of life due to prolonged healing times and increased infection risks. Current treatment modalities, including pharmacological, physical, and surgical interventions, often yield limited efficacy and adverse effects, highlighting the urgent need for novel therapeutic strategies. The objective of this research is to create SIKVAV-modified chitosan hydrogels with the intention of improving the process of skin wound healing in diabetic mice. We synthesized the hydrogels and established a diabetic mice model with skin wounding to evaluate its healing effects and underlying mechanisms. The results of our study indicate that the SIKVAV-modified chitosan hydrogels markedly enhance the wound healing process in diabetic mice. This effect may be attributed to several mechanisms, including differentiation of fibroblasts, proliferation of keratinocytes, the promotion of angiogenesis, stimulation of collagen synthesis, upregulation of growth factor expression, and possible involvement of the TGF-β1/Smad3 signaling pathway. This research not only provides a new biomaterial for the treatment of diabetic wounds but also elucidates the related molecular mechanisms involved in wound healing of DFUs, offering valuable insights for future clinical applications.