Head Capsule Measurements Research Articles

Biology and morphometric studies of beet armyworm, Spodoptera exigua (Hub.)

The study is mainly focused on the morphology of different stages i.e., egg, larvae, pupa and adult. The head capsule measurements (widest head width) were taken by use of Stereozoom binocular microscope. The beet armyworm undergoes 5 instars with a maximum and minimum head width of 0.26 ± 0.01 and 1.70 ± 0.05 mm respectively. The head capsule width of larval instars showed successive increase in a different ratio at each moult and did not follow regular geometrical progression (Dyar's law). Colour polymorphism was clearly observed in the fifth instar of S. exigua recording about 34 morphs with a range of colours i.e., green, black, pink and yellow being dominant with combination of colours and cannibalism also reported in the late instar stages. The total developmental period lasted for 32.38 ± 5.13 days.

Characterizing Billbug (Sphenophorus spp.) Seasonal Biology Using DNA Barcodes and a Simple Morphometric Analysis.

Simple SummaryBillbugs (Sphenophorus spp.) are a group of grass-feeding weevils considered to be one of the most important and widespread insect pests of turfgrass. However, our limited understanding of regional variation in billbug species composition and inability to identify the damaging larval stage to species level, has hindered our ability to resolve the seasonal biology of many billbug species and constrained development of effective management approaches. In this study, we developed a robust DNA barcoding approach for identification of morphologically cryptic billbug larvae. Using this molecular tool combined with larval head capsule measurements we characterized regional variation in billbug species and developed larval phenology charts. Our approach provides researchers with the molecular tools necessary to fill critical gaps in our understanding of billbug seasonal biology and will facilitate the development of improved turfgrass pest management programs.Billbugs (Sphenophorus spp.) are a complex of grass-feeding weevil species that reduce the aesthetic and functional qualities of turfgrass. Effective billbug monitoring and management programs rely on a clear understanding of their seasonal biology. However, our limited understanding of regional variation in the species compositions and seasonal biology of billbugs, stemming primarily from our inability to identify the damaging larval stage to species level, has hindered efforts to articulate efficient IPM strategies to growers. We used a combination of DNA barcoding methods and morphometric measures to begin filling critical gaps in our understanding of the seasonal biology of the billbug species complex across a broad geographic range. First, we developed a DNA barcoding reference library using cytochrome oxidase subunit 1 (COI) sequences from morphologically identified adult billbugs collected across Indiana, Missouri, Utah and Arizona. Next, we used our reference library for comparison and identification of unknown larval specimens collected across the growing season in Utah and Indiana. Finally, we combined our DNA barcoding approach with larval head capsule diameter, a proxy for developmental instar, to develop larval phenology charts. Adult COI sequences varied among billbug species, but variation was not influenced by geography, indicating that this locus alone was useful for resolving larval species identity. Overlaid with head capsule diameter data from specimens collected across the growing season, a better visualization of billbug species composition and seasonal biology emerged. This approach will provide researchers with the tools necessary to fill critical gaps in our understanding of billbug biology and facilitate the development of turfgrass pest management programs.

Developmental biology of Aspavia armigera (Fabricius, 1775) (Hemiptera: Pentatomidae) on rice (Oryza sativa L.) (Poaceae) and three other hosts in Nigeria

Abstract The developmental biology of Aspavia armigera on four rice varieties ITA 301, ITA 305, ITA 307 and ITA 315 was studied under ambient conditions at the Plant Biology Laboratory, Olabisi Onabanjo University, Ago-Iwoye, Nigeria. Alternative host plants were also used in the experiment. The results showed that the development of A. armigera depended on its hosts. Oviposition periods were 41.7 days, 30.8 days, 22.6 days and 18.1 days on rice varieties ITA 301, ITA 305, ITA 307 and ITA 315, respectively. Fecundity ranged from 80.3 eggs on ITA 301, 79.6 eggs on ITA 307, 76.1 eggs on ITA 305 and 65.6 eggs on ITA 315. The differences in fecundity were not statistically significant. The eggs incubated for 4 to 6 days before hatching into nymphs on all the varieties. The longevity of mated and unmated males and mated females on the rice varieties were not statistically different, while unmated females lived significantly longer on ITA 305. There were five nymphal instars, which persisted for 16.8-19.5 days depending on the variety. The developmental period was significantly (p<0.05) delayed on ITA 315, and the mean growth ratio of head capsule measurements was 1.27. Oviposition periods ranged between 5 and 20 days on cowpea and 6 and 18 days on rice. A significantly higher number of eggs (fecundity) was laid by mated females of A. armigera fed on soybean than those fed on cowpea. Mean developmental periods were 25.7, 25.5, 21.4, and 20.3 days on soybean, amaranthus, rice and cowpea, respectively. Mated females lived for a significantly shorter period on cowpea (21.9 days) than on the other hosts.

Description, Biology, and Impact of the Fruit-Feeding Moth, Mompha luteofascia sp. n. (Lepidoptera: Momphidae), on Miconia calvescens (Melastomataceae) in Costa Rica

AbstractFruits of Miconia calvescens DC. (Melastomataceae) were dissected to study insect frugivory in this plant in its native habitat in Costa Rica. Larvae of an unknown Mompha species (Lepidoptera: Momphidae), Anthonomus monostigma Champion (Coleoptera: Curculionidae), and unidentified Diptera and Hymenoptera were found in M. calvescens fruits. The Mompha species, described here as new as Mompha luteofascia Koster & Badenes-Pérez, was the most abundant insect frugivore in M. calvescens, infesting up to 38.1% of the fruits sampled. Feeding damage by M. luteofascia was positively correlated with fruit maturity, and resulted in significantly reduced numbers of seeds and rates of seed germination. Miconia calvescens fruits with medium damage (50–75% of the pulp missing) and high damage (75–100% of the pulp missing) had an average of 96 and 99 seeds, respectively, whereas undamaged fruits contained an average of 127 seeds per fruit. In fruits with medium and high fruit damage, only 1.9 and 0.1% of the seeds germinated, respectively, whereas 34.8% of the seeds germinated in undamaged fruits. Mompha luteofascia developed through three instars, as determined from measurement of head capsules. Larvae usually fed as one individual per each fruit attacked, exiting fruit to pupate in foliage or litter. Parasitism of M. luteofascia larvae was substantial, averaging 64 and 38% at the two study sites. Although host specificity of M. luteofascia has not been evaluated, the significant reductions in seed numbers and seed germination caused by its larvae indicate that this species deserves further assessment as a potential biological control agent of M. calvescens.

Biology and Laboratory Rearing of Codling Moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae) on Its Natural Host “Green Immature Apple” Malus domestica (Borkh) (Rosales: Rosaceae)

The
 codling moth, Cydia pomonella Linnaeus, 1758 (Lepidoptera: Tortricidae) is an economically
 important pest of apple trees in Turkey and also in worldwide. The aim of this
 study was to report a continuous rearing method on green immature apples (Malus domestica cv. 'Gala') for C. pomonella and fundamental information
 of its biology. The experiments were conducted from 2017 to 2019 under the
 laboratory conditions. Eggs were laid individually or disorderly in small
 masses on the oviposition substrate in the adult cages. Adult food had a
 positive effect on the number of eggs laid per female and egg viability. Larvae
 developed through five instars based on head capsule measurements. Larval development
 was completed in 15.89±0.25 days. Survival rate of larvae ranged from 24.1% and
 92.6% based on larval density per apple. The duration of pupal development was
 8.90±0.10 days with 87.5% adult emergence. The life cycle was completed on
 average in 29.5±0.3 days. Adult longevity was 12.2±0.60 days for
 females and 16.7±1.90 days for males. A single female laid about 12.3 eggs/day
 and a total of 85-200 eggs with 83.9% viability. The codling moth has been reared continuously in the laboratory for two years
 with no evidence of disease in the colony.

Development, growth and metabolic rate of Hermetia illucens larvae

AbstractThe larvae of Hermetia illucens are known to successfully bio‐convert a vast range of organic substrates into high protein and fat biomass, but little is known about the larval instars. During this research, larval head capsules and biomass growth were measured daily and the specific metabolic rate of larger instars were considered. The head capsule measurements revealed that H. illucens pass through 6 actively feeding larval stadia before entering the last nonfeeding but migrating 7th stadium. Larval growth follows a sigmoid curve with slowly accelerating growth in the earlier stadia and decelerating growth in the latest stadia. In contrast, development was fast until reaching stadium 6 and then slowed down. Accordingly, the specific metabolic rate was high in instars 3, 4 and 5 and reduced in instars 6 and 7.

Predicting Larval Stage Distribution of Lobesia botrana (Lepidoptera: Tortricidae) at Three Constant Temperatures.

European grapevine moth, Lobesia botrana (Denis and Schiffermüller), is an invasive pest species subject to regulatory control under the International Plant Protection Convention. An extensive infestation of this insect detected in California vineyards in October 2009 resulted in the initiation of postharvest research intended to ensure continuing trade of California table grapes by preventing the unintentional introduction of L. botrana to uninfested export destinations. Having the ability to accurately predict and confirm the particular stadia of larval development of insects obscured from direct observations, such as those being mass-reared in diet for use in phytosanitary control experiments, is necessary to be able to ensure that the most tolerant life stage is accurately targeted. Lobesia botrana were reared from egg to eclosion at three different temperatures: 16, 20, and 24°C (60% RH; 16:8 [L:D] h). Daily samples (≥15) were taken of larvae and pupae to determine the chronological distribution of life stages until daily samples comprised 100% pupae, after which only adults were counted. Head capsule measurements of all sampled larvae were taken (n = 2,820) and analyzed using nonlinear least squares (NLLS) fitting to Gaussian curves to describe stadia-specific head capsule demarcations and the probability of misclassifying instars due to overlapping life stage distributions.

Life History of the Emerald Jewel Wasp Ampulex compressa

The Emerald Jewel Wasp Ampulexcompressa (Fabricius) is an endoparasitoid of the American cockroach Periplanetaamericana (Linnaeus). Its host subjugation strategy is unusual in that envenomation is directed into the host central nervous system, eliciting a long-term behavior modification termed hypokinesia, turning stung cockroaches into a lethargic and compliant, but not paralyzed, living food supply for wasp offspring. A.compressa manipulates hypokinesic cockroaches into a burrow, where it oviposits a single egg onto a mesothoracic leg, hatching three days later. Herein we describe the life history and developmental timing of A.compressa. Using head capsule measurements and observations of mandibular morphology, we found that the larvae develop through three instars, the first two ectoparasitoid, and the third exclusively endoparasitoid. The first two instars have mandibles sufficient for piercing and cutting the cuticle respectively, while the third instar has a larger and blunter mandibular structure. During ecdysis to the third instar, the larva enters the body cavity of the cockroach, consuming internal tissues selectively, including fat body and skeletal muscle, but sparing the gut and Malpighian tubules. The developmental timing to pupation is similar between males and females, but cocoon volume and mass, and pupation duration are sexually dimorphic. Further, we show that the difference in cocoon mass and volume can be used to predict sex before eclosion, which is valuable for studies in venom pharmacology, as only females produce venom.

Biological Traits of Cadra cautella (Lepidoptera: Pyralidae) Reared on Khodari Date Fruits Under Different Temperature Regimes.

The almond moth, Cadra cautella (Walker) (Lepidoptera: Pyralidae), is a serious worldwide pest of dates and other crops, both in the field and in storage. Infestation by this pest significantly limits the sale and export of dates. Modified atmospheres, the temperature manipulations, are the best eco-friendly alternatives in stored product protection. We examined temperature regulation as an ecologically friendly method of pest management in stored food commodities, in particular, its effects on characteristics of C. cautella biology, including development time, oviposition period, fecundity, fertility, and longevity of adults reared on date cultivar 'Khodari,' at temperatures of 15, 25, and 35 ± 1 °C, with 65 ± 5% relative humidity and a photoperiod of 15:9 (L:D) h. Six larval instars were recorded at each temperature. Larval development was sluggish at 15 °C; therefore, daily observations were terminated after 180 d of the larval span for this temperature. Total larval spans of 48.95 ± 0.76 and 32.12 ± 1.08 d were recorded at 25 °C and 35 °C, respectively. A mean ovipositional period of 5.80 ± 0.44 d yielding 213.60 ± 13.41 eggs per female, with hatchability of 85.94 ± 2.97%, was recorded at 25 °C, whereas at 35 °C, a mean ovipositional period of 1.60 ± 0.26 d yielded 19.80 ± 5.11 eggs per female, with no hatchability. These differences were highly significant. Our observations of poor development at 15 °C, lower numbers of eggs and absence of hatchability at 35 °C, together with head capsule measurements and growth ratios, can be of benefit when planning management strategies to reduce C. cautella infestation.

Head Capsule Width is Useful for Determining Larval Instar inHeilipus lauri(Coleoptera: Curculionidae)

SummaryThe purpose of this study was to determine the number of larval instars for Heilipus lauri Boheman (Coleoptera: Curculionidae). We reared 385 larvae under laboratory conditions and measured the width of the larval head capsules from digital images. Data were analyzed using the Hcap program and Dyar’s rule. Analysis of the frequency distribution showed the existence of 4 distinct peaks. The mean Dyar’s constant was 1.42, where the relationship between the natural logarithm of the head capsule widths and the number of larval instars resulted in a near-perfect geometric growth for each instar. The excellent fit of the data to a linear model suggests that there was nearly no overlap among the 4 instars in the head capsule measurements, making it possible to distinguish the 4 instars of H. lauri accurately. SumarioEl objetivo de este estudio fue determinar el numero de estadios larvarios de Heilipus lauri Boheman (Coleoptera: Curculionidae). Un total de 385 larvas fueron criadas en condiciones de laboratorio. El ancho de la capsula cefalica de cada larva fue medido por medio de un sistema computarizado de analisis de imagenes. Los datos fueron analizados con el programa HCap y la regla de Dyar. El grafico de distribucion de frecuencias mostro cuatro picos de distribucion del ancho de la capsula cefalica. El promedio de la constante de Dyar fue de 1.42 donde la relacion entre el logaritmo natural del ancho de la capsula cefalica y el numero de estadio larvario resulto en un perfecto crecimiento geometrico de cada estadio larvario. El excelente ajuste lineal de las datos sugiere que no existen instares traslapados y es posible distinguir cuatro estadios larvarios en H. lauri.View this article in BioOne

Life History and Biology of the Elm Bark Beetle <i>Scolytus kashmirensis</i> Schedl (Coleoptera: Curculionidae: Scolytinae) Infesting <i>Ulmus villosa</i> in Kashmir

Elm bark beetle Scolytus kashmirensis is a key pest of elm nurseries in the social forestry ecosystem of Kashmir. The biological and morphological characters of this species were determined both in field and in the laboratory. The larvae remained inactive throughout the winter which resumed their activity from the third week of March. Pupation started from the first week of April and the first swarming adults appeared from the last week of April. After emergence, the adults fly to suitable trees and undergo maturation feeding for 8.4 (±0.68 SD) days and females laid 46 (±9.45 SD) eggs on an average. The eggs hatched after an incubation period of 11 - 13 days. The five larval instars were distinguished from each other on the basis of the width of head capsule measurements. The larvae completed their development in 40 - 46 days. The pupal stage lasted for 10 - 20 days and finally adults emerge that lived for 45 - 60 days. The single longitudinal maternal gallery measured 42.67 (±11.07 SD) mm (on average) long and the larval gallery was 43 (±10.96 SD) mm (on average) in length. The seasonal distribution of various life stages and the number of generations were also recorded.

REARING OF FIELD COLLECTED LARVAE OF THE MAHOGANY SHOOT BORER, Hypsipyla robusta (MOORE) ON AN ARTIFICIAL DIET A PRELIMINARY STUDY

Hypsipyla robusta (Morre) (Lepidoptera: Pyralidae) is an important pest of mahogany,Swietenia macrophylla (King) in Sri Lanka and world over. Larval attack at an earlystage of growth, leads to branching which reduces the quality and value of timber,significantly. Work is in progress to study the effect of shade on the growth anddevelopment of mahogany and attack by the shoot borer. This paper presents aspects ofdevelopment of shoot borer larvae reared on an artificial diet, as well as field informationrelated to damage and parasitism. Larvae collected from two field sites at fortnightintervals were reared in the laboratory on an artificial diet at 27-32° C. Development offield collected larvae were followed until the emergence of adults, during which duration ofthe developmental stages, adult sex ratio, parasitism level and mortality were recorded.Measurement of head capsule widths of larvae collected and their location on the shoot,was also made. A total of 774 larvae were collected and reared during the study, out ofwhich 120 Hypsipyla adults emerged giving a male: female sex ratio of 1:8. Nearly 40%of the field collected larvae were parasitized by Cotesia app. (Hymenoptera: Braconidae).Head capsule measurements indicated five larval instars that were found to infest differentparts of the growing shoot. Duration of development of larvae differed depending onwhether they were parasitized or not. Several problems encountered during rearing arediscussed.

Examination and revision of the larval life history of Microplitis croceipes (Hymenoptera: Braconidae)

Microplitis croceipes (Braconidae) is a solitary parasitoid of Heliothis virescens (Noctuidae), the tobacco budworm. The larva of M. crocepies develops within the abdominal cavity of its host while the host remains active. Larval development culminates with egression of the larva from its host, and subsequent cocoon spinning and pupation near the host’s body. Previous research indicated that M. croceipes larvae pass through three instars within the host, followed by egression and pupation. Head capsule measurements recorded in this study at several intervals during larval development revealed that the larvae of M. croceipes pass through five instars. The final molt, i.e. into the fifth instar, occurs during larval egression from the host, and the shed exuvium remains lodged in the egression wound, acting as a plug that prevents exsanguination of the host. The host remains alive through parasitoid pupation, and may play a defensive role guarding the parasitoid cocoon during its development to the adult stage.

Systematics and Biology ofCremastobombycia chromolaenae, New Species (Gracillariidae), a Natural Enemy ofChromolaena odorata(L.) King and H. Robinson (Asteraceae)

ABSTRACT. A new species, Cremastobombycia chromolaenae, is described from Florida and Texas, USA. The larva is a leaf miner on Chromolaena odorata (L.) King and H. Robinson, a 2–3 m tall perennial shrub of the family Asteraceae, whose native range extends from southern Florida south to northern Argentina (Fig. 1). The plant is also known to occur in Africa, Asia, and Australia where it is considered exotic and in some countries has become a major weed. The larvae of C. chromolaenae are hypometamorphic and possess two distinct larval body forms and feeding behaviors—an early stage sap-feeding form with a flattened body and prognathous mouthparts and a later stage tissue-feeding form with a more cylindrical body and possessing hypognathous mouthparts. Based on head capsule counts and measurements, the larvae undergo five instars, with the first three instars being of the sap-feeding form and the last two tissue-feeding instars. The larval mine begins as a short, serpentine track which enlarges to a rounded,...

Determination of the Life Cycle of the Olive Fruit Leaf Moth,Palpita unionalis (Lepidoptera:Pyralidae) in the Laboratory

Palpita unionalis (Hübner), the olive leaf moth, is one of the most important pests of olive nurseries and olive orchards in Turkey and also in the Mediterrean Basin generally. In this study, P. unionalis was reared at 24 ± 1 °C, 65% RH and 16:8 h L:D in the laboratory on leaves of its natural olive host plant, Oleae europeae cv. ‘Ayvalık’. Eggs were laid individually or in clusters on the underside of the olive leaves, but at random in the adult cages. There were 6 instars based on molts and head capsule measurements. Larval development was completed in an average of 23.35 ± 2.13 d and the survival rate was 60%. Adult longevity averaged 16.0 ± 1.57 d for females and 16.3 ± 1.21 d for males. The mean fecundity was 352 ± 42.9. Developmental period of immature stages, adults' life span, fecundity and sex ratios were determined in the laboratory.

Ciclo de vida y control de la mariposa asiática de los cítricos, Papilio demoleus (Lepidoptera: Papilionidae), una nueva plaga invasiva en Puerto Rico.

La llegada en el 2006 de la mariposa asiática de los cítricos Papilio demoleus L. a Puerto Rico ha generado interés en conocer detalles sobre su control y sobre su ciclo biológico. Este trabajo estuvo dirigido a describir su ciclo de vida bajo condiciones locales, y a determinar, mediante pruebas de laboratorio, qué plaguicidas biológicos o de riesgo reducido serían efectivos en el manejo de sus poblaciones. Encontramos que en Puerto Rico, la mariposa tuvo un ciclo de vida de alrededor de 30 días. La etapa de huevo dura tres días; los estadios de los estados larvales 1 al 5 son de 3, 2, 3,3 y 5 d, respectivamente; el estado de pupa, 12 días. Las cápsulas cefálicas de las hembras de último estado tienden a ser más grandes que las de los machos. Nuestros datos apuntan hacia la posibilidad de 12 o más generaciones al año de P. demoleus en Puerto Rico. Las pruebas de toxicidad aguda estomacal de terceros estados larvales indican que spinosad y Bacillus thuringiensis subs, kurstaki tienen mortalidades típicas de 100%. Otros plaguicidas probados, como la azadirachtina y una mezcla de piretrinas con rotenona, no fueron tan efectivos. Las posibles ventajas al uso de estos plaguicidas se discute a la luz de iniciativas para fomentar el uso de plaguicidas de "riesgo reducido" en cultivos como los cítricos.

Determination of Paronychiurus kimi (Collembola: Onychiuridae) age structures by head width measurements with reference to cadmium toxicity

The age structure of Paronychiurus kimi (Lee) was determined by analysis of the head capsule width distribution using a H CAP program. The H CAP program successfully separated five distinct peaks, which represented a single age group, with a low misclassification probability of 0.1. The mean head capsule widths for each age group were 0.123, 0.165, 0.258, 0.304 and 0.350 mm, respectively. The age groups of P. kimi could be subdivided into two postembryonic developmental stages, the juvenile stage (groups 1 and 2) and adult stage (groups 3–5), on the basis of stage at first reproduction of P. kimi. Based on this information, we examined the chronic effect of cadmium on the age structure of P. kimi. After 28-day chronic exposure to various cadmium concentrations, the total number of P. kimi individuals decreased in a concentration dependent manner. The proportions of the juvenile and adult stages to the total number of individuals in the cadmium treated soils were significantly different from those in the control. In addition, the overall proportions of age structures were affected by cadmium treatment. These results indicate that head capsule measurements can be used to determine the age group of P. kimi and chronic exposure of cadmium to P. kimi at the individual level may be translated and manifested at the population level, by changing the age structures of P. kimi populations.

Postembryonic development of the unique antenna of Mantophasmatodea (Insecta)

The postembryonic antennal development and life cycle of a member of the insect order Mantophasmatodea ( Lobatophasma redelinghuysense) was investigated using a series of annulus counts and a time sequence of head capsule measurements. The life cycle comprised six instars. Females achieved significantly larger head capsules from instar 2 onwards, resulting in adult females having a larger mean head capsule diameter (2.58 mm) than males (2.27 mm). Antennae of first instar larvae comprised a smooth four-segmented basiflagellum and a seven-segmented, sensilla-rich distiflagellum. Lengthening of the basiflagellum was achieved by the addition of two annuli per moult, generated by division of the basal annulus (meriston). Annulus number and the unique annulation pattern of the distiflagellum remained constant until adulthood. The segmentation pattern of adult antennae (comprising a basiflagellum and a distiflagellum of 14 and seven annuli respectively) and mode of antennal elongation was consistent for all 11 species examined. Subdivisions in basiflagellar annuli were observed in adults of all species examined, although they are not considered to be true annular divisions. The structure of the mantophasmatodean antenna appears to be autapomorphic within Insecta, bearing little resemblance to that of Grylloblattodea, Dictyoptera or Phasmatodea, all putative sister groups of the Mantophasmatodea. However, the mode of flagellar elongation most closely resembles that of Isoptera, Blattaria and Dermaptera.

Biology of the moth Dasyses rugosella Stainton a storage pest of yams (Dioscorea spp.) (Lepidoptera, Tineidae)

Egg laying generally commenced in less than 24 h of adult emergence. Unmated females did not lay any eggs until they had mated. Larval head capsule measurements and direct rearing experiments indicated that D. rugosella underwent 7 larval stages of which the last was the pre-pupa. The duration of development of the immature stages decreased with increased temperature. The lower threshold temperatures of development of the immature stages ranged from 15°C in the pupal stage to 19.4°C in the larval stage. Both fecundity and longevity of adult females were unaffected by feeding or food deprivation (starvation). Adults seemed to have relied on larval nutrition to sustain their biological activities. Tethered flight on pins showed that while males flew for about 22 sec on day 0, unmated and mated females flew for only 12 and 10 sec, respectively. Furthermore, males flew up to the 7th day of their life while unmated and mated females could fly only up to the 5th and 3rd days of their life, respectively.

Descrição dos estágios imaturos e biologia de Chinavia pengue (Rolston) (Hemiptera, Pentatomidae)

Adultos de Chinavia pengue (Rolston, 1983) foram coletados em Garopaba, SC, e criados em laboratório sob condições controladas (24 ± 1°C; UR 70 ± 10%; 12hL:12hE). Como alimento, adultos e ninfas receberam vagens verdes de feijão (Phaseolus vulgaris L.). Os ovos de C. pengue seguem o padrão de coloração e esculturação do cório e coloração e forma dos processos aero-micropilares descrito para as espécies neotropicais de Chinavia. Ninfas de 1º instar possuem uma mancha ovalada no dorso da cabeça e tórax, característica das espécies de Chinavia. Em C. pengue, essa mancha tem coloração laranja-avermelhada, e as manchas abdominais (4+4 manchas laterais e uma mediana) são brancas. Características exclusivas das ninfas de 2º a 5º ínstares de C. pengue são a coloração laranja-avermelhada das manchas do pro- e mesotórax e das manchas circulares no centro das placas abdominais laterais. Não se observou sobreposição nas medidas da largura da cabeça entre os cinco ínstares. Cada fêmea depositou 15,9 ± 4,18 posturas e 218,8 ± 48,60 ovos, sendo 14 ovos/postura o arranjo mais freqüente. A fertilidade foi de 70,0% ± 19,01; a mortalidade no 2º ao 5º estádio foi de 1,6% ± 4,49. A razão sexual obtida foi de 1 macho: 1 fêmea. A duração da fase imatura (ovo a adulto) foi de 45,7 ± 2,99 dias.