The objective of the present work was to define further the hormonal factor or factors which participate in the maintenance of primate luteal cells in culture, including both luteotropic and luteolytic processes. Corpora lutea (CL) were obtained from rhesus monkeys during midluteal phase (17–19 days after onset of menses) of the menstrual cycle and were dissociated with collagenase. In the first experiment the basic culture medium (F10 + 10% fetal calf serum) was supplemented by the nongonadotropin hormones insulin (I), thyroxin (T4), and cortisol (C), and/or cholesterol (Ch), in the presence or absence of human chorinoic gonadotropin (hCG). Concentrations of progesterone (P) and estradiol (E2) were determined by radioimmunoassay of unextracted samples. P synthesis by luteal cells in the basic medium progressively declined throughout the 10-day culture period. Although we were unable to stabilize P production under any condition, with the addition of I-T4-C and in the presence of hCG, P synthesis was significantly higher (P<0.05) throughout the 10-day incubation period. E2 production showed a similar declining pattern, regardless of the composition of the culture medium. Ch alone had no beneficial effects on maintenance of high levels of P secretion, whereas in the presence of hCG production of P was enhanced (P<0.01) for the first 2 days of culture. In the second experiment the combination of any two nongonadotropin hormones with hCG appeared equally beneficial during the first 2 days of culture, after which P secretion declined rapidly. In an additional experiment, luteal cells were initially cultured with I-T4-C for 2, 4, 6 or 8 days, at which times hCG was added for the remaining days of culture. When hCG was initially added on Days 2 or 4, P production was significantly elevated on Days 4 and 6, and on Day 6, respectively, followed by a gradual decline. The results demonstrate that the combination of nongonadotropin hormones, when in concert with hCG, is beneficial in maintaining P synthesis at higher levels for longer intervals. Addition of hCG slowed the declining pattern of P production when introduced on culture Days 2 or 4; nevertheless, the declining pattern of P output continued. The current state of this culture system does not permit in vitro imitation of chorionic gonadotropin-induced “rescue” of CL, as it occurs in the fertile menstrual cycle.