Hawaiian Mesopelagic Boundary Community Research Articles

Observations of the Hawaiian Mesopelagic Boundary Community in Daytime and Nighttime Habitats Using Estimated Backscatter

The Hawaiian mesopelagic boundary community is a slope-associated assemblage of micronekton that undergoes diel migrations along the slopes of the islands, residing at greater depths during the day and moving upslope to forage in shallower water at night. The timing of these migrations may be influenced by environmental factors such as moon phase or ambient light. To investigate the movements of this community, we examined echo intensity data from acoustic Doppler current profilers (ADCPs) deployed at shallow and deep sites on the southern slope of Oahu, Hawaii. Diel changes in echo intensity (and therefore in estimated backscatter) were observed and determined to be caused, at least in part, by the horizontal migration of the mesopelagic boundary community. Generalized additive modeling (GAM) was used to assess the impact of environmental factors on the migration timing. Sunset time and lunar illumination were found to be significant factors. Movement speeds of the mesopelagic boundary community were estimated at 1.25–1.99 km h-1 (35–55 cm s-1). The location at which the migrations were observed is the future site of a seawater air conditioning system, which will cause artificial upwelling at our shallow observation site and may cause animal entrainment at the seawater intake near our deep water observation site. This study is the first to observe the diel migration of the mesopelagic boundary community on southern Oahu in both deep and shallow parts of the habitat, and it is also the first to examine migration trends over long time scales, which allows a better assessment of the effects of seasons and lunar illumination on micronekton migrations. Understanding the driving mechanisms of mesopelagic boundary community behavior will increase our ability to assess and manage coastal ecosystems in the face of increasing anthropogenic impacts.

Broadband backscatter from individual Hawaiian mesopelagic boundary community animals with implications for spinner dolphin foraging

Broadband simulated dolphin echolocation signals were used to measure the ex situ backscatter properties of mesopelagic boundary community (MBC) in order to gain a better understanding of the echolocation process of spinner dolphins foraging on the MBC. Subjects were captured by trawling with a 2-m-opening Isaacs-Kidd Midwater Trawl. Backscatter measurements were conducted on the ship in a 2000 L seawater tank with the transducer placed on the bottom pointed upwards. Backscatter measurements were obtained in both the dorsal and lateral aspects for seven myctophids and only in the dorsal aspect for 16 more myctophids, six shrimps, and three squids. The echoes from the myctophids and shrimps usually had two highlights, one from the surface of the animal nearest the transducer and a second probably from the signal propagating through body of the subject and reflecting off the opposite surface of the animal. The squid echoes consisted mainly of a single highlight but sometimes had a low amplitude secondary highlight. The backscatter results were used to estimate the echolocation detection range for spinner dolphins foraging on the mesopelagic boundary community. The results were also compared with multi-frequency volume backscatter of the mesopelagic boundary community sound scattering layer.

Broadband backscatter measurements of individual Hawaiian mesopelagic boundary community animals

Broadband simulated dolphin echolocation signals were used to measure the ex situ backscatter properties of mesopelagic boundary community animals. The signal had a peak frequency of 100 kHz, 3-dB bandwidth of 35 kHz, and duration of 80μs. Subjects were captured by trawling with a 2-m-opening Isaacs-Kidd midwater trawl. Backscatter measurements were conducted on the deck of the ship in a 2000-L seawater plastic tank with the transducer placed on the bottom, pointed upwards. Anesthetized animals were suspended in the transducer’s beam by nylon monofilament lines connected to a support device constructed out of dowels. Backscatter measurements were obtained in both the dorsal and lateral aspects for seven myctophids and only in the dorsal aspect for the other 13 myctophids, 6 shrimps, and 3 squids. The echoes from the myctophids and shrimps usually had two highlights, one from the surface of the animal nearest the transducer and a second probably from the signal propagating through the body of the subject and reflecting off the opposite surface of the animal. The squid echoes consisted mainly of a single highlight but sometimes with a low-amplitude secondary highlight. The target strength had a rippled pattern in the frequency domain showing a distinct null.

Light and lunar cycle as cues to diel migration of a sound-scattering layer

The Hawaiian mesopelagic boundary community is an island-associated midwater scattering layer comprised of small fishes, shrimps, and squids that undergoes diel vertical as well as horizontal migrations. It has been hypothesized that light levels are an important cue or trigger for vertical migration and presumably, horizontal migration. The migration pattern of the scattering layer was measured over complete lunar cycles while the incident light levels were recorded. Due to differences in the rise and set times of the moon and cloud cover, light and lunar cycle were not completely coupled, allowing separation of the light effects of moon phase and other cues associated with lunar cycle. Four calibrated echosounder moorings were deployed with approximately even spacing, perpendicular to the leeward coast of Oahu. Moorings were deployed for one complete lunar cycle at each of three locations, recording 10 echoes every 15 min. Light sensors measured the nocturnal light intensity at 30-s intervals. Statistical analysis revealed significant effects of both light and other lunar cycle cues. Overall, the effect size was very low considering the light transmission characteristics of the subtropical Pacific, making measurement from stationary acoustic platforms critical.

Diel migration dynamics of an island-associated sound-scattering layer

The Hawaiian mesopelagic boundary community, consisting of island-associated, midwater sound-scattering layers, undergoes diel migrations with both vertical and horizontal components. To understand the dynamics of the community's migration at fine temporal scales, we utilized a bottom-mounted, 200-kHz active-acoustic mooring that transmitted 10 signals every 15 min, from dusk until dawn for 5 days. Five moorings were deployed 1.0–3.0 km from the leeward coast of Oahu in 0.5 km intervals. Two layers within the boundary community were observed to undergo simultaneous diel vertical and horizontal migration. The shallow layer came within 10 m of the surface and 1 km of the shoreline. The deeper layer remained 90 m from the surface and 2.5 km of the shoreline. Vertical migration rates were measured at 0–1.7 m min –1 while the horizontal rate averaged 1.7 km h –1, swamping the vertical movement. The turning point of the migration pattern was observed 45 min before the midpoint between sunset and sunrise. Until the migration's turning point, scattering strength increased relatively constantly as the animals migrated towards shore, with the highest scattering densities found in the shallowest areas at midnight. Total scattering strength measured at the leading and trailing edge of the layer support the hypothesis that increased animal densities nearshore are related to packing as mesopelagic animals avoid the surface and the bottom. We observed high levels of biomass moving rapidly, over a great distance, into shallow waters very close to shore providing insight into the significant link the mesopelagic boundary community provides between nearshore and oceanic systems.

Echo strength and density structure of Hawaiian mesopelagic boundary community patches.

A broadband sonar system and digital camera with strobe lights were mounted on a vertically profiling frame with a depth sensor. The echo strengths and densities of animals within individual mesopelagic boundary community patches were investigated as a function of depth. Time and distance from shore were also investigated. Simultaneous surface echosounder surveys permitted comparison of density estimates from two techniques. Echo strength values suggest nearshore boundary community animals are primarily myctophid fishes, which was confirmed by preliminary photographic evidence. Echo strength varied significantly as a function of distance from the shoreline and time. These measures of echo strength are important for estimating density from a surface echosounder. Density estimates from these revised echo strengths compare well with those made with echo highlight counting, which is independent of echo strength. These density measures suggest that previous density estimates were too low but do not change the conclusions of these studies. Vertical microstructure in density was apparent but animal size and compositional structure was not evident within a patch. Patch edges were abrupt, with no differences in the density or echo strength from patch interiors. These edges were generally straight, with a sharp drop in density to the background density of zero. Estimates of animal size as a function of time provide information about the diel migration patterns of these mesopelagic animals.

Target strength and density structure of Hawaiian mesopelagic boundary community patches

A broadband sonar system and digital camera with strobe lights were mounted on a vertically profiling frame with a depth sensor. The target strengths and densities of animals within individual mesopelagic boundary community patches were investigated as a function of depth. Simultaneous echosounder surveys permitted comparison of density estimates from echo‐energy integration and echo‐highlight counting. Target strength values suggest nearshore boundary community animals are primarily myctophid fishes which was confirmed by preliminary photographic evidence. Target strength varied significantly as a function of distance from the shoreline and time. Echo‐energy integration estimates of density made with these revised target strengths compare well with those made with echo highlight counting. These density measures show that previous density estimates were too low but do not change the conclusions of these studies. Vertical microstructure in density was apparent but animal size and compositional structure was not evident within a patch. Patch edges were abrupt, with no differences in the density or target strength from patch interiors. These edges were generally straight, with a sharp drop in density to the background density of zero. Estimates of animal size as a function of time provide information about the diel migration patterns of these mesopelagic animals.

Spatial dynamics of a nearshore, micronekton sound-scattering layer

AbstractIn the Hawaiian Islands, there is a distinct resident community of micronekton, distributed along a narrow band where the upper underwater slopes of the islands meet the oceanic, mesopelagic environment. This mesopelagic boundary community serves as an important food resource to many animals. The goal of this work was to examine spatial heterogeneity of the Hawaiian mesopelagic boundary biomass at a range of scales, in the context of its diel vertical and horizontal migrations. A modified echosounder was used to sample the coasts of three Hawaiian Islands, permitting a range of scales from several meters to several kilometers to be assessed rapidly. The Hawaiian mesopelagic boundary community fits the hierarchical model of patch structure with patches within patches that are part of a larger-scale matrix of patches. Large differences in the overall distribution patterns of the mesopelagic boundary community exist along with a wide range of overall mesopelagic-animal densities. High animal-density locations have boundary-community layers with a large (kilometers) horizontal extent, and low animal-density locations have small (tens of meters), discrete patches. Higher animal-density locations are also more complex than low-density sites, with more levels of patchiness within the same range of spatial scales. Both time of day and distance from shore significantly affected the geometric and density characteristics as well as the distribution of aggregations within the boundary layer. Horizontal and vertical structures of the mesopelagic boundary community are also coupled. In high-density sites, there is strong vertical layering in acoustic-scattering strength while in low-density sites vertical acoustic structure is absent. The differences observed in the distribution of the mesopelagic boundary community at different levels of overall mesopelagic-animal density suggest biological forcing as the dominant mechanism. A description of heterogeneity in the mesopelagic boundary community in Hawaii is the first step in understanding its importance to both neritic and oceanic ecosystems and its potential for linking these two systems.

Fine-scale diel migration dynamics of an island-associated sound-scattering layer

The Hawaiian mesopelagic boundary community, an island-associated, midwater sound-scattering layer, undergoes diel vertical and horizontal migrations. To understand the dynamics of the community’s migration at small temporal scales and large spatial scales, five bottom-mounted, 200-kHz active-acoustic mooring that transmitted ten signals every 15 min, from dusk until dawn for 5 days. Two layers within the boundary community were observed to undergo simultaneous diel vertical and horizontal migration. Vertical migration rates were measured at 0–1.7 m/min, while the horizontal rate averaged 1.67 km/h, swamping the vertical movement. The vertex of the migration pattern was observed 45 min before the midpoint between sunset and sunrise. Until the vertex, animal density increased relatively constantly as the animals migrated towards shore, with the highest animal densities found in the shallowest areas at midnight. Animal abundance estimates at the leading and trailing edge of the layer support the hypothesis that increased animal densities near shore are related to packing, as mesopelagic animals avoid the surface and the bottom. We observed high levels of biomass moving rapidly, over a great distance, into shallow waters very close to shore, providing insight into the significant link the mesopelagic boundary community provides between near-shore and oceanic systems.

Energy: converting from acoustic to biological resource units.

Acoustic backscattering strength is often used as an index of biomass; however, the relationship between these variables has not been directly validated. Relationships were investigated between acoustic cross section at 200 kHz, measured as part of a previous study, and measured values of length, biovolume, dry weight, ash-free dry weight, and caloric content of the same individual specimens. Animals were part of the Hawaiian mesopelagic boundary community and included shrimps, squids, and myctophid fishes. The strong relationships found between all the variables measured make it possible to approximate any one variable from the measured values of others within a class of animals. The data show that for these midwater animals, acoustic scattering can be used as an index of biomass. Dorsal-aspect acoustic cross section at 200 kHz predicted dry weight and ash-free dry weight at least as well as did body length, a standard predictor. Dorsal-aspect acoustic cross section at 200 kHz was also a strong predictor of total caloric content. The relationship between dorsal-aspect acoustic cross section and caloric content of Hawaiian mesopelagic animals was linear and additive. Consequently, it is possible to directly convert acoustic energy from these animals to organic resource units without having knowledge of the size distribution of the populations being studied.

Target strength measurements of Hawaiian mesopelagic boundary community animals

A 200-kHz echosounder modified to digitize the envelope of the received echoes directly into a computer was used to measure the ex situ target strength (TS) of live animals from the Hawaiian mesopelagic boundary community as a function of animal size, tilt and roll angle, and biological classification. Dorsal aspect TS (in dB//1 m) at 200 kHz was related to the animal’s length: myctophid fish TS=20 log (standard length in cm)−58.8, r2=0.91, squid TS=18.8 log (mantle length in cm)−61.7, r2=0.81, shrimp TS=19.4 log (length in cm)−74.1, r2=0.83. Tilting the fish 5° and 10° changed the measured TS by up to 3.0 dB, decreasing TS as the fish was tilted forward and increasing TS as the fish was tilted backwards. In shrimp, forward tilt increased TS while backward tilt decreased TS by up to 3.3 dB. No consistent trend in squid TS change was observed with tilt angle. Roll angles of 5° and 10° increased the TS of all groups by up to 3.0 dB. Myctophid lateral aspect TS was consistently about 6 dB higher than the dorsal TS. Physiological analysis of the fishes’ swimbladders revealed that the swimbladder is not the dominant scattering mechanism in the myctophid fishes studied.

Diel horizontal migration of the Hawaiian mesopelagic boundary community observed acoustically

The mesopelagic boundary community off the leeward coasts of 2 Hawaiian Islands, Oahu and Hawaii, was investigated with an echosounder modified to read directly into a laptop com- puter. Acoustic sampling was conducted over a total distance of 12.6 km off the Waianae coast of Oahu and 46.3 km off the Kona coast of Hawaii. The density of organisms was determined using echo energy integration, and relative abundance was determined in a way analogous to catch-per-unit- effort. The vertical range of mesopelagic organisms expanded as the mesopelagic layer rose and then compressed as it descended. The vertical range of the layer off the Kona coast was larger than that off the Waianae coast, possibly because of the greater bottom depth off Kona. Near midnight, the bound- ary community 3 km from the shoreline was split into 2 distinct layers, one beginning approximately 25 m from the surface and one beginning approximately 90 m from the surface. The density and the relative abundance of mesopelagic organisms were consistently higher off the Waianae coast than the Kona coast. However, the density of organisms observed in both locations was high, reaching a maximum of 1800 organisms m -3 off Waianae and 700 organisms m -3 off Kona. The maximum relative abundance off Waianae neared 100%, while off Kona it never exceeded 70%. In both locations, organisms were found within 1 km of shore, in waters much shallower than their assumed daytime habitat. The temporal patterns of relative abundance and density of organisms in waters closest to the shores of each island resembled a bell curve, with a peak in relative abundance and density around midnight. In waters further from shore, the temporal patterns in relative abundance and density had a bimodal distribution, with peaks around both 21:00 and 03:00 h. These patterns in relative abun- dance and density are significantly affected by the distance of the sampling location from the shore- line, but not by the depth of the sampling site. The data suggest that the organisms of the mesopelagic boundary community undergo a diel horizontal migration that is reciprocal, 1.8 km in 2 h toward shore and then the same distance in approximately the same amount of time away from shore, in addition to their well-established vertical migration. The temporal patterns of the horizontal com- ponent of the migration are predictable and are conserved between days, phases of the moon, sea- sons, and islands.

Acoustic investigation of the Hawaiian mesopelagic boundary community

A 200 kHz echosounder modified to sample directly into a computer was used to survey the mesopelagic boundary community (MBC) in waters off the leeward coasts of three Hawaiian Islands from dusk to dawn. The target strengths of individual mesopelagic organisms captured in Isaacs-Kidd midwater trawls were measured with the echosounder in a shipboard seawater tank for use in echo-energy integration calculations. Acoustic survey data were mapped in ArcView, allowing analysis of spatial structure. The MBC is spatially heterogeneous both horizontally and vertically. The parameters of patch structure vary significantly throughout a night and between islands. The density of mesopelagic organisms was consistently higher off the coasts of Oahu and Lanai than the coast of Hawaii. However, the density of organisms observed in all locations was high, reaching a maximum of 1800 organisms/m3 off Oahu and 700 organisms/m3 off Hawaii. Patterns in density were significantly affected by the location’s distance from the shoreline, but not by its depth. Patterns in density suggest that the MBC undergoes a significant diel horizontal migration in addition to its well-documented vertical migration. Due to this predictable and conserved horizontal migration, the MBC is found much closer to shore than previously reported.

Cephalopod paralarvae assemblages in Hawaiian Islands waters

The distribution and abundance of cephalopod paralarvae near the Hawaiian Islands are described. Paralarvae were collected during 5 plankton surveys in 1991 to 1993. The 404 tows at 59 stations collected 10375 paralarvae from 21 families and 57 species. The most numerous families were the Ommastrephidae (23% of total catch), Pyroteuthididae (17%), Enoploteuthididae (16%), Onychoteuthididae (14 %), and Chtenopterygidae (8 %). The most numerous species were Ommastrephes bartramii (18%), Pterygioteuthis microlampas (15%), Chtenopteryx sicula (8%), and Onychoteuthis compacta (6%). Analysis of paralarval distribution patterns identified 2 paralarval assemblages: 'island associated' and 'oceanic'. The 15 'island-associated' species showed increased paralarval abundance near the islands, suggesting preferential spawning in this area. Epipelagic nearshore spawners included Onychoteuthis sp. C, Sthenoteuthis oualaniensis, and Nototodarus hawaiiensis. Mesopelagic nearshore spawners included 3 reported members of the Hawaiian Mesopelagic Boundary Community (Abralia trigonura, Liocranchia reinhardti, and Chiroteuthis picteti) and 3 probable new members (Liocranchia valdiviae, Histioteuthis hoylei and Enoploteuthis jonesi).