Anaplasmosis is endemic in cattle in several areas of the USA, including eastern and central Oklahoma. This rickettsia-induced hemolytic disease caused by Anaplasma marginale commonly occurs during warm months when arthropod vectors, both biological (ticks) and mechanical (biting flies), are abundant. Mechanical transmission by blood-contaminated fomites can occur any time of the year when infected reservoir hosts are present. In January 1995, clinical anaplasmosis was confirmed in a group of 117 cattle in a pasture (unit A) of a larger herd in western Oklahoma (Harper County). A cluster of 5 animals had signs of severe disease within a 2-week period. Each of the affected cattle was treated and recovered. The outbreak was surprising because January is not the usual season for vector activity in Oklahoma and neither clinical anaplasmosis nor seropositivity to the complement fixation (CF) test occur with frequency in cattle from western Oklahoma, even during vector season. The problem was investigated further because of the unusual occurrence of the outbreak and especially because the cattle were infested with the winter tick, Dermacentor albipictus. The role of D. albipictus in transmission of anaplasmosis has not been well documented, especially in Oklahoma. An on-site investigation was conducted on February 1, 1995, 12 days after the first cow was observed to be ill. The herd was observed generally, and several animals were examined carefully. Clotted and unclotted blood samples were collected from 9 cows: 8 randomly selected and 1 known to have had anaplasmosis. Winter ticks (partially engorged nymphs and adults) were collected from all 9 animals. Extensive inquiry into management eventually revealed that the owner had used a nondisinfected saw to dehorn an unspecified number of animals on December 10, 1994, approximately 35 days prior to the onset of clinical anaplasmosis. Thus, the outbreak appears to have resulted from the owner’s dehorning procedure. The tight cluster of cases of disease that occurred soon after surgery suggests exposure to a common source of infection, most likely a reasonably highly parasitemic carrier. Six of the 9 cattle from which blood samples were collected were seronegative by the CF test for A. marginale, 1 was a suspect (< 1:5), and 2 were reactors (Table 1). Reactors included the 1 cow known to have been clinically affected. Randomly selected ticks collected from the 9 cows were split in half with a sharp razor blade; salivary gland from
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