3D-3D registration of facial models, has great advantages in personal individual identification in forensic medicine. However, orthodontic treatment has brought changes in facial soft and hard tissues, which has a potential effect in personal identification. The aim of the study was to explore whether orthodontic treatment affects 3D-3D facial recognition. A total of 68 patients aged between 18 and 38 were selected (30 subjects with tooth extraction, 38 subjects with non-tooth extraction) and a control group consisting of 30 volunteers without orthodontic treatment was selected from a database of 3dMD facial models. 3dMD facial model acquisition was performed twice for each subject. For both extraction and non-extraction groups, T0 was acquired before treatment, and T1 was acquired at the end of treatment. The time span of 2 times 3dMD facial model acquisition in the control group was about 2 years. 3dMD facial models were then registered onto other 3dMD models belonging to the same and different individuals according to the minimum point-to-point distance, getting a cohort of matches and mismatches. Root mean square (RMS) value of the minimum point-to-point distance between two models was then calculated. The intra- and inter-observer repeatability coefficients were 0.986 (P<0.001) and 0.982 (P<0.001). The absolute technical error of measurement (TEM) value was 0.05 mm and 0.04 mm, and relative technical error of measurement (rTEM) value was 4.91 % and 3.60 %, respectively. Possible significant differences between groups were assessed through independent samples t-test or Mann–Whitney U test (p<0.001). The average RMS value was 1.03±0.33 mm in matches of orthodontic group, 0.67±0.22 mm in matches of control group, and 2.66±0.51 mm in mismatches. An RMS value range of 1.70–1.77 mm could distinguish matches from mismatches in 100 % of cases in the present study. This study showed that orthodontic treatment would not affect 3D-3D facial recognition in adults for the purpose of individual identification in forensic medicine.
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