Respiratory syncytial virus (RSV) is known as a major cause of respiratory tract infection in adults and children. Human to human transmission occurs via droplets as well as direct and indirect contact (e.g. contaminated surfaces or hands of medical staff). Therefore, applicable hygiene measures and knowledge about viral inactivation are of utmost importance. Here, we evaluated the virucidal efficacy of oral rinses specifically designed for children, WHO-recommended hand rub formulations and ethanol as well as 2-propanol against RSV in a quantitative suspension test (EN14476). Furthermore, we assessed the stability of RSV on stainless steel discs and investigated its inactivation by different surface disinfectants (EN16777). We observed that all tested oral rinses except one reduced infectious viral titres to the lower limit of quantification. The two WHO-recommended hand rub formulations as well as 30% ethanol and 2-propanol completely abolished the detection of infectious virus. Infectious RSV could still be recovered after several days on stainless steel discs. However, RSV could efficiently be inactivated by all tested surface disinfectants based on alcohol, aldehyde, or hydrogen peroxide. In conclusion, oral rinses, all tested hand rub formulations as well as surface inactivation reagents were sufficient for RSV inactivation in vitro.
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