Handling Practices Research Articles

Food handling practices and sanitary conditions of charitable food assistance programs in eThekwini District, KwaZulu-Natal, South Africa

Unsafe food handling practices by food handlers have dire health and financial implications worldwide. Each year, approximately 600 million people, or about 1 in 10 people, are said to become ill from eating contaminated food, and 420,000 people inadvertently die. According to the 2019 World Bank report on the economic burden of foodborne diseases, the annual cost of treating foodborne illnesses is estimated to be US$ 15 billion, and the total productivity loss caused by foodborne diseases in low- and middle-income countries is estimated to be US$ 95.2 billion annually. The purpose of this study was to assess the food handling practices and sanitary conditions of the charitable food assistance programs (CFAPs) in the eThekwini District of KwaZulu-Natal, South Africa. A descriptive cross-sectional study was conducted among 196 CFAPs in eight study settings across five municipal planning regions (MPRs) of the eThekwini District between January 2021 and May 2021. Data were collected using a standardized 37-item observational checklist and analysed through Stata Statistical Software (TX: StataCorp. 2021 LLC.: Release 17. College Station). Compliance levels were calculated using the compliance score (C-score), whereby 0.0–0.20 (0–20%), 0.21–0.40 (21–40%), 0.41–0.60 (41–60%), 0.61–0.80 (61–80%), and 0.81–1.00 (81–100%) were determined as very poor, poor, average, good, and very good, respectively. Statistically significant associations were declared at p < 0.05. Compliance with food hygiene, storage, and packaging was very poor (C-score = 0.003), as were personal hygiene and staff facilities (C-score = 0.147), as well as product information/labelling (C-score = 0.003). Similarly, waste management and pest control systems (C-score = 0.203), compliance with health and hygiene education/training (C-score = 0.335), as well as use and maintenance of transport (C-score = 0.333), all scored ‘poor’. Only the design of premises and facilities had an average compliance score (C-score = 0.43). Given CFAPs’ role in mitigating the impact of poverty, their strict compliance with hygiene protocols is of utmost importance. Systems for identifying and correcting common noncompliance in CFAPs are required.

Farmers’ Perception on Pesticide Use and Pest Management of Vegetable Crops: In the Upper Blue Nile Basin, Ethiopia, in the Case of Fogera Plain

This study examines the impact of training on smallholder farmers&amp;apos; pesticide handling practices, perceptions, and behaviors, with a focus on the correlation between training and several key factors, including pesticide storage, pest identification skills, pest management strategies, pesticide application frequency, information sources, protective equipment use, pesticide mixing habits, and disposal of empty pesticide containers. The research highlights that training plays a significant role in improving farmers&amp;apos; pesticide handling practices, although some areas still require additional education or interventions for further improvement. Significant associations were found between training and pest identification abilities. Farmers who received training were more adept at recognizing pests such as onion thrips (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 17.130, p &amp;lt; .001), downy mildew (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 10.221, p = 0.001), fruit borers (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 26.246, p &amp;lt; .001), and white flies (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 3.226, p = 0.072) compared to untrained farmers. Trained farmers were also more likely to seek reliable information from extension workers (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 13.18, p &amp;lt; 0.001), chemical dealers (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 15.44, p &amp;lt; 0.001), personal experience (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 8.03, p = 0.005), and product labels (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 14.36, p &amp;lt; 0.001), whereas untrained farmers were more inclined to guess or rely on informal sources (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 21.85, p &amp;lt; 0.001). Regarding pesticide storage, trained farmers were more likely to store pesticides safely, away from living areas, children, and animals, whereas untrained farmers often stored pesticides indoors. Training also affected mixing practices, with trained farmers less likely to mix pesticides near water sources (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 6.4, p = 0.01) and more likely to mix them in the field (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 51.38, p &amp;lt; 0.001). However, there were no significant differences in practices such as mixing pesticides according to the recommended doses (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 3.15, p = 0.08) or mixing different types of pesticides (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 0.31, p = 0.58). Trained farmers demonstrated better adherence to safety protocols, such as using personal protective equipment (PPE) and avoiding pesticide mixing at home. They were also more likely to triple-rinse spray tanks and avoid discharging pesticides into irrigation ditches. However, no significant differences were observed in reading pesticide instructions before spraying (X&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; = 0.24, p = 0.63), indicating a need for further emphasis on this aspect during training. Overall, the study confirms that training significantly improves safe pesticide handling but suggests areas for further education and intervention.

Práticas higiênico-sanitárias em feiras de arte e gastronomia de Niterói/RJ, Brasil

Introduction: Street food is an important market segment with an impact on the generation of jobs, income, and appreciation of food culture. However, it poses a challenge for public health because it increases the risk of transmission of pathogens from foodborne disease. Objective: Evaluate the hygienic-sanitary practices of food handlers at fairs and the physical-functional conditions of gastronomy and arts fairs in the city of Niterói/RJ. Method: The descriptive methodology was applied, using a qualitative and quantitative approach. For data collection, two scripts were developed and applied to evaluate the functional conditions of the fairs and the hygienic-sanitary practices of the handlers during sales, and a questionnaire was administered to outline the socioeconomic profile of the workers. Results: 68.1% were women; average income of 3 minimum wages; 51.1% had completed higher education; 46.8% had never taken a Good Food Handling Practices course; 70.5% did not use hair protection; and 86.8% used adornments. In 40% of the fairs visited, there was the presence of animals/vectors, as well as the absence of toilet facilities for handlers. Conclusions: Thus, despite its socioeconomic importance, the street food trade represents a potential health risk. Therefore, it is necessary to expand the hygienic-sanitary training of handlers and improve the environmental conditions of fairs.

Induced Resistance in Fruit and Vegetable Enhancing Host Defence to Curve Post-harvest Diseases

Induced resistance (IR) is an emerging, environmentally friendly strategy to manage post-harvest diseases in fruits and vegetables by enhancing the plant’s innate immune system. This approach leverages the plant's natural defense mechanisms, including the activation of defense-related enzymes like chitinases, glucanases, and peroxidases, accumulation of secondary metabolites such as phytoalexins and phenolics, and the strengthening of cell walls to prevent pathogen invasion. Additionally, the production of reactive oxygen species (ROS) plays a crucial role in both signaling and direct pathogen inhibition. Despite its potential, the effectiveness of IR varies across different crops and pathogens, and its success is heavily influenced by environmental factors, such as humidity, temperature, and light. There are also concerns regarding possible trade-offs, such as reduced yield or changes in fruit quality due to the diversion of energy towards defense responses. However, the integration of IR with other disease management strategies, including the use of biological control agents and conventional fungicides, has shown promising results in reducing post-harvest losses. Case studies in fruits like apples, tomatoes, and citrus, as well as vegetables like potatoes and peppers, demonstrate the practical applications of IR in commercial agriculture. Advances in genetic engineering are opening new pathways for enhancing IR by manipulating key genes involved in defense pathways, while new formulations and precision agriculture technologies offer greater control and efficiency in IR applications. These innovations, along with optimized post-harvest handling and storage practices, hold the potential to make IR a more reliable and sustainable solution for managing post-harvest diseases. However, ongoing research is necessary to address the variability in effectiveness and to explore long-term sustainability, especially as pathogens evolve and environmental conditions change. Induced resistance, when integrated with a comprehensive disease management approach, offers a significant step forward in reducing post-harvest losses and improving food security globally.

Impact of Various Washing Protocols on the Mitigation of Escherichia coli Contamination in Raw Salad Vegetables.

Vegetables are an essential component of a balanced diet. The consumption of ready-to-eat foods may lead to the risk of infections and illnesses due to microbial contamination. To mitigate the potential of microbial contamination risks, it is critical to promote safe handling practices among consumers. In this study, our research evaluated the efficacy of different vegetable washing methods, specifically with lettuce, tomato, and cucumber, to establish optimal practices for reducing microbial contamination. This study consisted of two phases. Initially, a survey was distributed to 150 volunteers using snowball sampling to assess everyday vegetable handling and washing methods. The survey's results identified four predominant methods: washing with a 5% vinegar solution for 3 min followed by tap water rinse (37.3% of participants), rinsing with tap water for 1 min (29.3%), washing with a 5% salt solution (vegetable soap) for 3 min followed by a tap water rinse (16.6%), and a 3 min tap water rinse (14%). A minor segment (3.33%) reported not washing their vegetables at all. The survey's findings guided the second phase, which tested the aforementioned washing protocols' effectiveness in reducing Escherichia coli (E. coli) levels on spiked contaminated salad vegetables. The tested vegetables were sterilized using UV light, inoculated with 0.5 McFarland E. coli, and then washed using the four identified methods. After that, E. coli enumeration after washing was performed using 3M™ Petrifilm and the comparison was analyzed via one-way ANOVA. During this study, it was revealed that the cucumbers had the highest E. coli contamination levels in comparison to the lettuce and tomato after washing. Interestingly, by comparing the three washing methods, it was found that washing the vegetables with vinegar proved to be the most effective solution for reducing microbial presence on both lettuce and cucumbers. Notably, the natural smoothness of tomato skin led to no significant differences in contamination levels across washing methods. In summary, vinegar washing effectively reduces microbial contamination from salad vegetables, highlighting the need for informed consumer practices to prevent foodborne outbreaks. This study emphasizes the importance of monitoring contamination sources and using safe washing techniques.

RETAINING WALL PLANNING IN WASTE MANAGEMENT

This study aims to analyze the performance and productivity of heavy equipment as well as the volume of waste produced in a waste disposal area (TPSA) located in Kuningan Regency. The research was carried out at the Ciniru TPSA located in Jalaksana District, Kuningang Regency, with the aim of finding out the data on the volumes of waste, transport trucks, and excavator heavy equipment. The results show that the amount of existing waste in the area reaches 630 m3 with an average amount of irritation of 71 times per day. In addition, the production of heavy machinery for garbage trucks, excavators, and bulldozers reaches 128.07 m3/hour, which is a working time of 8 hours and operational costs of Rp 244,957/hour, respectively. The findings of this study offer valuable information on the effectiveness of retaining walls in mitigating environmental impact, protecting agricultural areas, and improving waste handling practices. Moreover, investigating methods to extend landfill lifespan through improved spatial planning and capacity forecasting will be crucial, especially for regions facing waste overflow challenges in the near future.

Investigation of Shiga toxin-producing Escherichia coli and evaluation of the microbiological quality of raw vegetable sal-ads

Foodborne diseases (FBD) are a significant public health concern worldwide, affecting millions of people annually. Symptoms of FBD range from mild gastrointestinal discomfort to more severe conditions, which in some cases can lead to death. Raw vegetables, especially those consumed in ready-to-eat (RTE) salads, are particularly susceptible to microbial contamination. This study aimed to investigate the presence of Shiga toxin-producing Escherichia coli (STEC) and to evaluate the microbiological quality of 77 RTE raw vegetable salads from 20 restaurants in Niterói, RJ, Brazil. None samples presented STEC, nor the safety indicators (SI) Salmonella spp. and Listeria monocytogenes. However, 89.6% of the samples were considered unfit for human consumption, since they presented at least one hygiene indicator (HI) outside the established standards. The majority of samples (95.5%) were unsatisfactory for Enterobacteriaceae (EB), followed by total aerobic bacteria (TAB) (76.6%), thermotolerant coliforms (C45) (32.5%) and Escherichia coli (EC) (1.3%). The difference between the occurrences of hygiene indicators was not significant. Given the high percentage of inadequate samples found in the study, it is clear that there is a need for stricter surveillance and adequate hygiene practices in restaurants. To reduce the risks of microbial contamination in RTE raw vegetable salads, several approaches can be implemented. These include continuous training of food handlers, implementation of food safety management systems such as Hazard Analysis and Critical Control Points (HACCP), and regular inspections. In addition, consumers also play an important role by demanding safe food and following safe food handling and consumption practices at home.

BACTERIOLOGICAL AND CHEMICAL ASSESSMENT OF RAW MILK SOLD IN SÃO RAIMUNDO DAS MANGABEIRAS, MARANHÃO, BRAZIL

Owing to its nutrient content, milk is among the most consumed foods; however, milk can be easily contaminated or adulterated because of inadequate handling and hygiene practices during its production chain. Therefore, in this study, we aimed to evaluate the bacteriological, and chemical compositions of raw milk sold in São Raimundo das Mangabeiras in Northeast Brazil. Ten samples were obtained between November 2022 and June 2023. Mesophilic bacteria were enumerated, total (TC) and thermotolerant (TTC) coliforms were quantified, and the presence of Escherichia coli and Salmonella spp. were evaluated. Lactose (%), protein (%), fat (%), and added water (%) contents were also determined. Results showed mesophilic bacterial growth in 80% of our samples, with the highest value being 1×103 colony-forming units (CFU)/mL. In contrast, 100% of our samples contained TC and TTC, with the highest values ranging from 150–210 most probable number (MPN)/mL. None of the samples contained Salmonella spp.; however, one sample (10%) contained E. coli. Moreover, 80% of our samples had low fat, 30% low lactose, and 20% low protein content. In addition, 1% added water was detected in one sample (10%). Therefore, some raw milk sold in São Raimundo das Mangabeiras are not suitable for consumption, indicating inadequate handling of this food product.

Modelling a Western Lifestyle in Mice: A Novel Approach to Eradicating Aerobic Spore-Forming Bacteria from the Colonic Microbiome and Assessing Long-Term Clinical Outcomes.

The environmentally acquired aerobic spore-forming (EAS-Fs) bacteria that are ubiquitous in nature (e.g., soil) are transient colonisers of the mammalian gastro-intestinal tract. Without regular exposure, their numbers quickly diminish. These species of bacteria have been suggested to be essential to the normal functioning of metabolic and immunogenic health. The modern Western lifestyle restricts exposure to these EAS-Fs, possibly explaining part of the pathogenesis of many Western diseases. To date, the only animal studies that address specific microbiome modelling are based around germ-free animals. We have designed a new animal model that specifically restricts exposure to environmental sources of bacteria. A new protocol, termed Super Clean, which involves housing mice in autoclaved individually ventilated cages (IVCs), with autoclaved food/water and strict ascetic handling practice was first experimentally validated. The quantification of EAS-Fs was assessed by heat-treating faecal samples and measuring colony-forming units (CFUs). This was then compared to mice in standard conditions. Mice were housed in their respective groups from birth until 18 months. Stool samples were taken throughout the experiment to assess for abundance in transiently acquired environmental bacteria. Clinical, biochemical, histological, and gene expression markers were analysed for diabetes, hypercholesterolaemia, obesity, inflammatory bowel disease, and non-alcoholic fatty liver disease (the "diseases of the West"). Our results show that stringent adherence to the Super Clean protocol produces a significantly decreased abundance of aerobic spore-forming Bacillota after 21 days. This microbiomic shift was correlated with significantly increased levels of obesity and impaired glucose metabolism. There was no evidence of colitis, liver disease or hypercholesterolaemia. This new murine model successfully isolates EAS-Fs and has potential utility for future research, allowing for an investigation into the clinical impact of living in relative hygienic conditions.

Complementary feeding and food handling practices among caregivers in a semi-urban setting in Northern Uganda

Background: Complementary feeding practices influence the nutritional status and health of children, especially in developing countries where sub-optimal infant feeding practices are the major cause of childhood undernutrition, morbidity, and mortality. This study sought to characterize the adequacy of complimentary feeding and food handling practices among caregivers of children aged 6-23 months in a semi-urban setting. Methods: Exploratory cross-sectional study was conducted in Adyel division, Lira district in Northern Uganda among 127 child-caregiver pairs randomly selected from three villages. Caregivers were interviewed about their child-feeding practices, complementary foods used, and common practices in handling and preparation of foods used as complementary feeds. Results: Overall, children’s feeding patterns met the recommended dietary intake when assessed using the child feeding index (CFI) whereby children aged 6-8, 9-11, and 12-23 months scored 8.03, 9.17, and 7.59, respectively. However, most children were complemented with foods from the household meals and dietary diversity was limited by frequent use of food leftover from the previous meals. Conclusions: Inappropriate meal patterns and food handling practices identified are likely to compromise child nutrition and health. Nutrition education is needed to improve the caregivers’ child-feeding and food handling practices within their socio-economic and cultural context. Keywords: Complementary feeding practices; food handling; diet quality; food safety; children; Uganda.

Assessment of the Present Status of Milk Handling and Management Practices in Some Areas of Bangladesh

Milk contamination by dairy workers' spoiled hands, unclean utensils, microbes, and polluted water has caused many milk-borne epidemics. Most milk-borne zoonoses are also contracted by drinking infected milk. Bangladesh lacks milking hygiene and farmer awareness, and the milk cleanliness of dairy farmers awareness and practices in different regions was accessed. This study was aimed at documenting the present milking and management practices of dairy producers in particular districts of Bangladesh. From June 2020 to June 2022, 300 small dairy cow owners in twelve areas were surveyed using a preset questionnaire. Results revealed that male respondents (59.5%) outnumbered female respondents (41.33%) across various age categories. Most farmers milked animals at the shed and washed their hands before milking. Over half of dairy farmers cleansed the udder and teat before milking. More than two-thirds of farmers let the calf suckle before and after milking, while 98.02 percent used concentrate feed for teat massage. Most farmers (95.58%) manually milked cows twice daily. The majority of farmers sold their milk (77.25%) rather than drink it (10.17%). The study found that 93.92 percent of milkmen cleaned the milking pail using water, while 3.5-2.5 percent utilized sand, ash, or detergent. Only 53.66% of dairy farmers washed their cows' udders and teats after milking, which may not be aware of potential contamination from dipping teats in sterile cream. Mastitis prevention was not practiced by 85.42 percent of the respondents. Four to eight percent of producers know about antibiotics, milk adulteration, milk safety, and food safety issues. The application of scientific management approaches to milking is still lacking in the study areas. Therefore, training programs on improved milking management procedures can help farmers produce clean milk, increase dairy animal production, and increase farming income.

Assessing foodborne outbreak risks in Chinese households: A national survey analysis of pork handling practices

ObjectiveThis study aimed to assess the risk of foodborne outbreaks associated with household food handling practices in China and identify the consumer groups that are at higher risk for foodborne outbreaks. MethodsA national survey of household pork handling practices was conducted in China. The risk of foodborne outbreaks stemming from consumers’ improper handling practices was assessed utilizing the Weighted Harmonic Outbreak Prevention Index (WHOPI). The index categorized these practices into three levels of good hygienic practice (GHP): low (WHOPI≤0.35), medium (WHOPI: 0.36–0.70), and high (WHOPI>0.7). Logistic regression analyses were used to identify groups at higher risk for foodborne outbreaks. ResultsA total of 2730 households from seven provinces in mainland China were analyzed. Based on WHOPI, only 0.4 % of households demonstrated a high level of GHP, while 55.4 % showed a low level of GHP. The primary non-conforming stage was separation practices, followed by cooking and purchasing practices. Nearly two-thirds of households (64.8 %) didn’t have a separate cutting board for raw meat, and 44.9 % of them claimed to use the same board to handle ready-to-eats afterward. Logistic regression indicated more attention is required for those living in southern and rural areas, working in non-public institutions, possessing lower levels of education, and being single or widowed. ConclusionThe household food handling practices of Chinese consumers involve serious food safety risks. Education programs and interventions concerning safe handling practices in households are urgently needed.

Impact of pneumatic tube transportation on the aggregation of monoclonal antibodies in clinical practice.

Postproduction handling and in-hospital transportation of antibody drugs cause mechanical stress, including interfacial and shear stress, that can induce antibody unfolding and aggregation. The handling practices differ significantly between hospitals and the impact on protein stability is unknown. For example, the mechanical stress caused by transport via pneumatic tube systems (PTS) on therapeutic antibody aggregation is a potential safety and quality gap. The aim of this study was to investigate whether mechanical stress and PTS transportation in a hospital cause aggregation of five commonly used antibody drugs diluted in infusion bags. Orthogonal analytical methods showed that the handling and PTS transportation in this hospital did not cause aggregation of the investigated mAbs. The absence of aggregation could be explained by the reduction of interfacial stress due to headspace removal from the infusion bags and a mechanical sensor indicated that there was also only a moderate amount of mechanical stress caused by transportation with this particular PTS. Although this case study focuses on five antibody drugs and the practices in one hospital, the work demonstrates how to evaluate whether other handling and transportation practices cause significant mechanical stress that could compromise the quality and safety of antibody drugs.

The use of digital health services to combat E-waste health hazards: A review on the impact and awareness in Southwest Nigeria

The rapid advancement of technology has led to a significant increase in electronic waste (e-waste), posing serious health and environmental risks, particularly in developing regions like Southwest Nigeria. This review explores the utilization of digital health services to combat the health hazards associated with e-waste exposure. Digital health technologies, including mobile health applications, telemedicine, and electronic health records, play a critical role in raising awareness about e-waste toxicity, monitoring health impacts, and providing remote healthcare services to at-risk populations. This article discusses the effectiveness of these technologies in promoting safer e-waste handling practices and mitigating health risks, highlighting their potential in supporting public health interventions in underserved areas. The study underscores the need for strategic investments and policy support to enhance the adoption of digital health solutions in managing e-waste hazards by analyzing current challenges and opportunities, ultimately contributing to better health outcomes and environmental sustainability.

Assessment of Equipment Handling Practices in the Hospitality Industry: A Case Study of Kathmandu’s Key Departments

This study investigates equipment handling practices within the hospitality industry in Kathmandu, focusing on the frequency of use, types of equipment handled, training provided, and safety measures observed. A descriptive research design was employed, utilizing a quantitative survey approach that targeted 125 participants across three key departments: food production, food and beverage service, and housekeeping. Random sampling techniques were applied to conduct in-depth inspections, evaluate staff training, and assess the documentation and maintenance routines. The findings reveal that a significant portion of staff handles equipment daily, with food production equipment being the most commonly used. While training on equipment handling is regularly provided, issues such as the lack of written guidelines, limited access to personal protective equipment (PPE), and infrequent maintenance checks were identified as critical concerns. Additionally, safety protocols, particularly for heavy equipment, and the clarity of operational instructions were found to be inadequate in some cases. The study underscores the importance of consistent training, proper documentation, and the implementation of safety protocols to enhance equipment handling practices in the hospitality sector.

An outbreak of acute diarrhoeal disease caused by Shigella sonnei in a village in Dibrugarh district, Assam.

Background & objectives Food and waterborne illnesses remain a neglected public health issue in India. Events with large gatherings frequently witness outbreaks of acute diarrheal diseases due to consumption of contaminated food or water or poor food handling practices. In the present study, an outbreak of acute diarrhoeal disease (ADD) occurring among the attendees of a birthday party in rural Dibrugarh district in the northeastern Indian State of Assam was investigated. Methods Sociodemographic information along with details of ADD outbreak that included information about source of foods, food handlers, illness details, etc., were collected using an outbreak investigation form for descriptive and analytical epidemiology. Rectal swabs from affected individuals and food handlers were collected along with bore-well water samples and tested in the laboratory by performing bacterial culture, biochemical analysis and polymerase chain reaction. Due to the delayed report on the outbreak, collecting leftover food for laboratory testing and analysis was impossible. Results A total of 25 cases of ADD had similar signs and symptoms. The mean incubation period for developing acute diarrhoea was 26.36±8.76 (± standard deviation) hours from food consumption. The overall attack rate was 60.04 per cent (25/41); 20 per cent (5/25) required hospitalization. Thirteen rectal swab samples were tested for pathogens and found positive for Shigella sonnei. Antibiotic susceptibility test of isolated S. sonnei showed resistance to nalidixic acid, ciprofloxacin and cefotaxime. Consumption of one of the food items - chicken curry was significantly associated with illness (Odds Ratio=14.8; 95% Confidence Interval: 2.75-85.11); P value<0.05 and Population Attributable Fraction (PAF) was 70.18 per cent. The water samples were found satisfactory for human consumption. Interpretation & conclusions The findings suggested that S. sonnei infection could be implicated in the investigated food-borne diarrhoeal disease outbreak and that there was a potential for human-poultry cross-infection. Additionally, the study revealed concerning levels of S. sonnei resistance to recommended antibiotics and drew attention to their public health relevance.

Unveils key proteins in Xinjiang goat muscle linked to post-mortem meat quality: A TMT-based proteomic analysis

An extensive proteomic analysis utilizing the tandem mass tag (TMT) method was conducted to investigate the changes in protein expression in the longissimus dorsi muscle of Xinjiang goats over various post-mortem intervals: immediately after death within 0 h, 12 h, 24 h and 48 h. The investigation carefully identified around 108 proteins that showed significant changes in expression during these intervals. Among these proteins, six were highlighted for their crucial roles in muscle growth and differentiation of muscle fibers post-mortem. These proteins, namely COL12A1, MRPL46, CTNNB1, MYH1, CAPZA1, and MYL9, have a direct effect on the meat's quality attributes, such as tenderness and color. Further discuss observed a progressive increase in the expression of proteins linked with oxidative metabolism (MSRB2, ENOX1, LOC102170282, GSTM1, and AOC3) as the post-mortem aging period extended, particularly between 24 h to 48 h. These proteins are instrumental in defining the color and flavor profiles of goat meat, underscoring the importance of precise processing and storage conditions to preserve meat quality during the critical aging phase. This enhanced understanding of protein expression dynamics offers significant implications for optimizing meat quality and provides a scientific basis for post-mortem handling practices in the goat meat industry.

Non-typhoidal Salmonella among slaughterhouse workers and in the pork value chain in selected districts of Uganda.

Non-typhoidal Salmonella (NTS) is a major cause of gastroenteritis worldwide, often associated with meat consumption and meat processing. Research on NTS infection and circulating serovars in meat value chains in Uganda is limited. We aimed to establish NTS prevalence, antimicrobial resistance, and risk factors among slaughterhouse workers, and to identify potentially zoonotic serovars in the pork value chain. We conducted a nationwide cross-sectional survey, collecting 364 stool samples from livestock slaughterhouse workers and 1,535 samples from the pork value chain: mesenteric lymph nodes, fecal samples, swabs of carcass splitting floor, cleaning water, meat handlers hand swabs, carcass swabs, raw pork, cooked pork, and mixed raw vegetables. Samples were cultured for isolation of NTS, and subsequently serotyped according to White-Kauffmann-Le Minor scheme. Antimicrobial resistance profiles were determined using tube microdilution and Sensititre® EUVSEC3® plates. Semi- structured questionnaires with 35 questions were used to collect data on demographics, work related risk factors and activities outside the slaughterhouse. Overall NTS prevalence was 19.2% (365/1899). Proportions at slaughter were; 46.7% in floor swabs, 30.5% in carcass swabs, 20.5% in pig faeces,19.2% in mesenteric lymph nodes,18.4% in hand swabs, 9.5% in water and 5.2% in slaughterhouse workers. At retail, proportions were 33.8% in pork chopping surface, 33.1% in raw pork, 18.9% in hand swabs, 4.0% in cooked pork and 0.7% in vegetables. Sixty-one serovars were identified, with significant overlap between humans and the pork value chain. Overall, zoonotic S. Zanzibar, monophasic serovars of S. subspecies salamae (II) and subspecies enterica (I), S. Typhimurium and S. Newport, were the most prevalent. S. Typhimurium was predominant in humans and exhibited multi-drug resistance. NTS infection was significantly associated with eating, drinking, or smoking while working (OR = 1.95, 95% CI: 0.67-2.90%, p = 0.004). The detected NTS serovars in slaughterhouse workers could be a potential indicator of circulating serovars in the general population. The persistent presence of NTS along the pork value chain highlights occurrence of cross-contamination and the potential for transmission to consumers and slaughterhouse workers. This emphasizes the need to reduce Salmonella prevalence on pig farms and improve hygiene and pork handling practices at slaughter and retail points.

Microbiological quality assessment of exposed &lt;i&gt;Garri&lt;/i&gt; sold in selected markets in Oye-Ekiti, Nigeria

A common Nigerian fermented staple meal, garri can become contaminated during manufacturing, marketing and storage by a variety of germs, which could be harmful to consumers' health. In this work, isolates from garri sold in Oye-Ekiti Local Government Area were phenotypically and molecularly characterized. Nine samples were purchased from three different markets using sterile paper bags and examined in the laboratory. Based on morphological characteristics and biochemicaltests, the organisms isolated and their frequency of occurrence were Staphylococcus spp. (15 %), Bacillus spp. (70 %) and Enterobacter spp. (15 %) and Aspergillus spp. (100 %). Molecular characterization of two distinct Bacillus spp. identified them as Bacillus subtilis and Brevibacillus brevis exhibiting 85.9 % and 98.8 % percentage similarities, respectively. In order to minimize the level of contamination of garri by hazardous microorganisms, local processors of the product should be trained and retrained on food handling, food packaging, sanitation and hygienic practices.

Risk Factors for Bacterial Keratitis and Severe Disease in Hydrogel Contact Lens Users: A Multicenter Case-Control Study and Case-Only Analysis.

To assess risk factors for contact lens (CL)-related bacterial keratitis, cases and high-risk controls were enrolled. Using high-risk controls can help elucidate whether certain CL types or modalities are attributable to disease burden if risky wear patterns are similar between the cases and controls. This analysis identified whether such CL factors were associated with the occurrence of bacterial keratitis. In addition, a case-only analysis determined CL factors associated with severe disease. From 2018 to 2021, 158 controls were enrolled at University Hospitals of Cleveland Eye Institute, and 153 bacterial keratitis cases were enrolled across 14 sites in the United States. Cases were soft CL wearers with either culture-proven bacterial keratitis or a corneal infiltrate with an overlying epithelial defect within the central 4 mm of the cornea, uveitis, or significant pain. Fungal, protozoan, or nonsoft CL wear-related microbial keratitis cases were excluded. Controls were recruited from high-risk CL wearers with no history of disease. All participants completed a questionnaire related to demographics, type of CL used, wearing schedule, lens handling practices, and storage case handling. Cases with ulcer/infiltrate size ≥2 mm in size, presence of hypopyon, or had fortified antibiotics prescribed were classified as severe keratitis. Univariate and multivariable logistic regression was used to assess association of CL variables with the occurrence of bacterial keratitis as well as occurrence of severe disease among the cases only. Compared with the control cohort, cases were older (mean age 45.6 vs. 38.9 years), had more males (42.5% vs. 23.6%), and had more current or former smokers (41.7% vs. 12.9%). There were no significant associations between CL material (silicone hydrogel vs. not) or CL type (daily disposable vs. reusable) and occurrence of bacterial keratitis. More than two-thirds (67.3%) of cases were classified as severe. Among cases only, univariate analyses found current smokers to have increased risk of severe disease (OR=2.87; 95% CI 1.13-7.26, P=0.03). Adjusting for age, sex, and smoking among the cases only, daily disposable lenses were protective against severe disease (OR=0.32; 95% CI 0.11-0.89, P=0.03). Reusable lenses increased risk of severe microbial keratitis between 3.0- and 4.4-fold compared with compliant daily disposability. Compared with a high-risk control cohort, no specific lens factors were associated with occurrence of CL-associated bacterial keratitis. Among cases only, current smokers and patients wearing reusable lenses are at increased risk of severe keratitis. Daily disposable lenses were protective even when noncompliance to daily disposability was considered.