Halotydeus Destructor Research Articles

Adapting pest management strategies to changing climates for the redlegged earth mite, Halotydeus destructor

As climate change continues to modify temperature and rainfall patterns, risks from pests and diseases may vary as shifting temperature and moisture conditions affect the life history, activity, and distribution of invertebrates and diseases. The potential consequences of changing climate on pest management strategies must be understood for control measures to adapt to new environmental conditions. The redlegged earth mite (RLEM; Halotydeus destructor [Tucker]) is a major economic pest that attacks pastures and grain crops across southern Australia and is typically controlled by pesticides. TIMERITE® is a management strategy that relies on estimating the optimal timing (the TIMERITE® date) for effective chemical control of RLEM populations in spring. In this study, we assessed the efficacy of control at the TIMERITE® date from 1990 to 2020 across southern Australia using a simulation approach that incorporates historical climatic data and field experimental data on life history, seasonal abundance, and population level pesticide responses. We demonstrate that moisture and temperature conditions affect the life history of RLEM and that changes in the past three decades have gradually diminished the efficacy of the TIMERITE® strategy. Furthermore, we show that by incorporating improved climatic data into predictions and shifting the timing of control to earlier in the year, control outcomes can be improved and are more stable across changing climates. This research emphasises the importance of accounting for dynamic environmental responses when developing and implementing pest management strategies to ensure their long-term effectiveness. Suggested modifications to estimating the TIMERITE® date will help farmers maintain RLEM control outcomes amidst increasingly variable climatic conditions.

Ace and ace-like genes of invasive redlegged earth mite: copy number variation, target-site mutations, and their associations with organophosphate insensitivity.

Invasive Australian populations of redlegged earth mite, Halotydeus destructor (Tucker), are evolving increasing organophosphate resistance. In addition to the canonical ace gene, the target gene of organophosphates, the H. destructor genome contains many radiated ace-like genes that vary in copy number and amino acid sequence. In this work, we characterise copy number and target-site mutation variation at the canonical ace and ace-like genes and test for potential associations with organophosphate insensitivity. This was achieved through comparisons of whole-genome pool-seq data from alive and dead mites following organophosphate exposure. A combination of increased copy number and target-site mutations at the canonical ace was associated with organophosphate insensitivity in H. destructor. Resistant populations were segregating for G119S, A201S, F331Y at the canonical ace. A subset of populations also had copy numbers of canonical ace > 2, which potentially helps overexpress proteins carrying these target-site mutations. Haplotypes possessing different copy numbers and target-site mutations of the canonical ace gene may be under selection across H. destructor populations. We also detected some evidence that increases in copy number of radiated ace-like genes are associated with organophosphate insensitivity, which might suggest potential roles in sequestration or breakdown of organophosphates. Different combinations of target-site mutations and (or) copy number variation in the canonical ace and ace-like genes may provide non-convergent ways for H. destructor to respond to organophosphate selection. However, these changes may only play a partial role in organophosphate insensitivity, which appears to have a polygenic architecture. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

From laboratory to field: laboratory-measured pesticide resistance reflects outcomes of field-based control in the redlegged earth mite, Halotydeus destructor

Resistance to pesticides is typically identified via laboratory bioassays after field control failures are observed, but the results of such assays are rarely validated through experiments under field conditions. Such validation is particularly important when only a low-to-moderate level of resistance is detected in the laboratory. Here we undertake such a validation for organophosphate resistance in the agricultural pest mite Halotydeus destructor, in which low-to-moderate levels of resistance to organophosphorus pesticides have evolved in Australia. Using data from laboratory bioassays, we show that resistance to the organophosphate chlorpyrifos is higher (around 100-fold) than resistance to another organophosphate, omethoate (around 7-fold). In field trials, both these chemicals were found to effectively control pesticide-susceptible populations of H. destructor. However, when applied to a resistant mite population in the field, the effectiveness of chlorpyrifos was substantially decreased. In contrast, omethoate remained effective when tested alone or as a mixture with chlorpyrifos. We also show that two novel (non-pesticide) treatments, molasses and wood vinegar, are ineffective in controlling H. destructor when sprayed to pasture fields at rates of 4 L/ha. These findings suggest a close link between levels of resistance quantified through laboratory bioassays and the field effectiveness of pesticides; however, in the case of H. destructor, this does not necessarily mean all field populations possessing organophosphate resistance will respond similarly given the potentially complex nature of the underlying resistance mechanism(s).

Fitness costs of pyrethroid resistance in the polyphagous pest mite, Halotydeus destructor, under field conditions

The redlegged earth mite, Halotydeus destructor, is an economically important pest of winter field crops and pastures in Australia, and has evolved field resistance to pyrethroid chemicals through a polymorphism in the voltage-gated parasodium channel leading to knockdown resistance (kdr). In this study, we quantified the rate of reversion to susceptibility of partially resistant H. destructor populations under field conditions in the absence of pesticide exposure. This was conducted over multiple years at two geographically distant locations with mite populations known to possess pyrethroid resistance. Fitness costs associated with pyrethroid resistance were identified through reductions in the frequency of kdr resistance alleles in the absence of pesticides. This was assessed using an amplicon sequencing approach targeting known resistance alleles. We also found that resistance can increase rapidly in frequency after only a single pyrethroid application in the field. Our results highlight that, once established in H. destructor populations, pyrethroid resistance will not easily be lost even after several years, emphasizing the importance of limiting the evolution of resistance in the first place. This helps to explain why pyrethroid resistance in H. destructor continues to persist at very high frequencies in the field and continues to expand within Australia despite the existence of fitness costs. Understanding field fitness costs associated with pesticide resistance is important when devising resistance management strategies for this pest.

The redlegged earth mite draft genome provides new insights into pesticide resistance evolution and demography in its invasive Australian range.

Genomic data provide valuable insights into pest management issues such as resistance evolution, historical patterns of pest invasions and ongoing population dynamics. We assembled the first reference genome for the redlegged earth mite, Halotydeus destructor (Tucker, 1925), to investigate adaptation to pesticide pressures and demography in its invasive Australian range using whole-genome pool-seq data from regionally distributed populations. Our reference genome comprises 132 autosomal contigs, with a total length of 48.90 Mb. We observed a large complex of ace genes, which has presumably evolved from a long history of organophosphate selection in H. destructor and may contribute towards organophosphate resistance through copy number variation, target-site mutations and structural variants. In the putative ancestral H. destructor ace gene, we identified three target-site mutations (G119S, A201S and F331Y) segregating in organophosphate-resistant populations. Additionally, we identified two new para sodium channel gene mutations (L925I and F1020Y) that may contribute to pyrethroid resistance. Regional structuring observed in population genomic analyses indicates that gene flow in H. destructor does not homogenize populations across large geographic distances. However, our demographic analyses were equivocal on the magnitude of gene flow; the short invasion history of H. destructor makes it difficult to distinguish scenarios of complete isolation vs. ongoing migration. Nonetheless, we identified clear signatures of reduced genetic diversity and smaller inferred effective population sizes in eastern vs. western populations, which is consistent with the stepping-stone invasion pathway of this pest in Australia. These new insights will inform development of diagnostic genetic markers of resistance, further investigation into the multifaceted organophosphate resistance mechanism and predictive modelling of resistance evolution and spread.

Warmer temperatures reduce chemical tolerance in the redlegged earth mite (Halotydeus destructor), an invasive winter-active pest.

BACKGROUNDQuantifying how chemical tolerance of pest arthropods varies with temperature is important for understanding the outcomes of chemical control, for measuring and monitoring resistance, and for predicting how pesticide resistance will evolve under future climate change. We studied the redlegged earth mite, Halotydeus destructor (Tucker), a winter‐active invasive agricultural pest in Australia. Using a replicated block experiment, we tested the effect of different thermal conditions on the expression of chemical tolerance to a pyrethroid and two organophosphates. Our chemical bioassays were conducted on two redlegged earth mite populations: one possessed organophosphate resistance, whilst the other was susceptible to pesticides. Mites were first acclimated at cool (4 °C) and warm (14 °C) conditions and then exposed to pesticides in both cool (11 °C) and warm (18 °C) test conditions.RESULTSWarm test conditions generally reduced chemical tolerance to all pesticides relative to cool test conditions. Median lethal dose (LD50) values of mites tested under cool conditions were 1.12–3.57‐fold greater than of mites tested under warm conditions. Acclimation had a variable and small impact on chemical responses. Thermal factors (ratio between test temperatures) were similar between populations for each active ingredient. Despite reduced chemical tolerances under warm test conditions for individual mite populations, resistance factors (ratio between resistant and susceptible mite populations) were relatively consistent.CONCLUSIONOur data provides context for prior theoretical work demonstrating climatically constrained pesticide resistances in Australian redlegged earth mites. Estimates of temperature dependent toxicity measured in this study may be useful in parameterizing models of redlegged earth mite control under an increasingly warm and more variable climate. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

The mitogenome of Halotydeus destructor (Tucker) and its relationships with other trombidiform mites as inferred from nucleotide sequences and gene arrangements.

The redlegged earth mite, Halotydeus destructor (Tucker, 1925: Trombidiformes, Eupodoidea, Penthaleidae), is an invasive mite species. In Australia, this mite has become a pest of winter pastures and grain crops. We report the complete mitogenome for H. destructor, the first to represent the family Penthaleidae, superfamily Eupodoidea. The mitogenome of H. destructor is 14,691 bp in size, and has a GC content of 27.87%, 13 protein‐coding genes, two rRNA genes, and 22 tRNA genes. We explored evolutionary relationships of H. destructor with other members of the Trombidiformes using phylogenetic analyses of nucleotide sequences and the order of protein‐coding and rRNA genes. We found strong, consistent support for the superfamily Tydeoidea being the sister taxon to the superfamily Eupodoidea based on nucleotide sequences and gene arrangements. Moreover, the gene arrangements of Eupodoidea and Tydeoidea are not only identical to each other but also identical to that of the hypothesized arthropod ancestor, showing a high level of conservatism in the mitogenomic structure of these mite superfamilies. Our study illustrates the utility of gene arrangements for providing complementary information to nucleotide sequences with respect to inferring the evolutionary relationships of species within the order Trombidiformes. The mitogenome of H. destructor provides a valuable resource for further population genetic studies of this important agricultural pest. Given the co‐occurrence of closely related, morphologically similar Penthaleidae mites with H. destructor in the field, a complete mitogenome provides new opportunities to develop metabarcoding tools to study mite diversity in agro‐ecosystems. Moreover, the H. destructor mitogenome fills an important taxonomic gap that will facilitate further study of trombidiform mite evolution.

Strip spraying delays pyrethroid resistance in the redlegged earth mite, Halotydeus destructor: a novel refuge strategy.

Pesticide resistance has seen control options for the redlegged earth mite (RLEM), Halotydeus destructor, dwindle for Australian grain farmers. The recent discovery of high recessiveness for pyrethroid resistance in RLEM provided an opportunity to examine the feasibility of a refuge strategy to slow the evolution of resistance. Unlike lepidopterous pests in Bt crops, where refuge strategies are routinely practiced, RLEM is a slow-moving pest, which will impact the design of susceptible refuges. Firstly, we confirmed the pyrethroid resistant allele is recessive to the susceptible (wildtype) allele (in terms of resistance) across spatially separated Australian populations. Secondly, we demonstrated that a small, localized resistant mite population can revert to susceptibility at field relevant scales and conditions. Next, we used a simulation modelling approach to design a practical refuge strategy to maintain susceptibility to pyrethroids in populations with a low incidence of resistance. Certain configurations (e.g. a pesticide strip width of 50 m and refuge spacing of 10m) maintained low levels of resistance across a 10-year time horizon, with lower mite abundance and minimal yield loss. A larger refuge proportion did not always delay resistance, and, under certain conditions, increased resistance frequency. Strip spraying to maintain refuges can be readily incorporated into RLEM management programs where sprayer widths in commercial cropping contexts are typically between 20-40 m. A refuge approach to RLEM management that uses strip spraying may enhance long term control options in the absence of new chemical registrations but will now require field validation. © 2021 Society of Chemical Industry.

Fitness Costs Associated with Pyrethroid Resistance in Halotydeus destructor (Tucker) (Acari: Penthaleidae) Elucidated Through Semi-field Trials.

Pyrethroid resistance in the redlegged earth mite, Halotydeus destructor (Tucker), is primarily attributed to a kdr (knockdown resistance) mutation in the parasodium channel gene. To assess fitness costs associated with this resistance, adult resistant and susceptible populations were mixed in different proportions in microcosm tubs and placed in a shade-house simulating field conditions. Three separate experiments were undertaken whereby parental mites were collected from the field and offspring were followed for two to three generations. The association between fitness costs and kdr-mediated resistance was investigated by examining differences in mite numbers and changes in resistant allele frequencies across generations. In two (of the three) experiments, the population fitness measure of mites was significantly lower in microcosms containing a higher proportion of resistant individuals compared with treatments containing susceptible mites. No differences in mite fitness were observed between treatments in the third experiment; in this instance, the starting proportion of individuals homozygous for the resistant mutation was much lower (~40%) than in the other experiments (>90%). In all three experiments, a decrease in the resistant allele frequency across mite generations was observed. These findings indicate a potential deleterious pleiotropic effect of the kdr mutation on the fitness of H. destructor and have implications for resistance management strategies aimed at this important agricultural pest. Further experiments investigating fitness costs directly in the field are warranted.

Learnings from over a decade of increasing pesticide resistance in the redlegged earth mite, Halotydeus destructor (Tucker).

The redlegged earth mite, Halotydeus destructor (Tucker), is a destructive and economically important pest of winter grain crops and pastures in Australia. It is largely controlled by pesticides, but this mite has evolved resistance to pyrethroid and organophosphate chemicals. A national Resistance Management Strategy has been developed for pro-active management to delay further resistance evolution, though its success is reliant on a detailed understanding of the incidence, patterns of spread, current distribution and the nature of resistance in the field. Here, we report on a long-term resistance surveillance programme undertaken between 2006 and 2019 informed by resistance risk forecasting. By mapping the Australian distribution of resistance through time, we show that resistance is present across three Australian states and covers more than 3000 km. This current range includes a recently identified population exhibiting organophosphate resistance representing the most easterly location of resistance in H. destructor. Using field history information, we identify associations for the first time between crop management practices employed by farmers and the presence of pyrethroid resistance. Management strategies that could minimize the risk of further resistance include limiting local spread of resistance through farm hygiene practices, crop rotations and reducing pesticide usage. This study highlights the challenges of resistance in H. destructor but also indicates how quantitative resistance risk analysis can be developed to target field surveillance and delay further resistance. The management strategies highlighted in this study can help maintain the effectiveness of control options but will depend on farmer engagement and adoption. © 2021 Society of Chemical Industry.

Options for managing pesticide resistance in the redlegged earth mite (

Chemical strategies to manage pest populations that have evolved pesticide resistance can involve killing resistant individuals by using higher rates of the same pesticide or applying alternative pesticides for which resistance does not exist. However, the dilemma is that alternative pesticides available for many pest species are limited, while higher pesticide rates may lead to the evolution of higher resistance levels. Here, both strategies are tested in a resistant population of the redlegged earth mite, Halotydeus destructor, which contained resistance against two groups of chemicals: synthetic pyrethroids and organophosphates. Resistant mites were introduced into exclusion field plots, which were then treated with pyrethroid, organophosphate or alternative chemicals for 2 years at a low pressure (one spray at the registered field rate per year) or a high pressure (two sprays at the registered field rate per year). A single pyrethroid application suppressed mite numbers, but pyrethroid-resistant allele (kdr) frequencies quickly rose from ∼50% to nearly 100%. Thereafter, pyrethroid chemicals lost effectiveness. However, kdr frequencies declined across mite generations when pyrethroids were not used, regardless of other treatments. Organophosphates continued to suppress mite populations under both high and low pressures, irrespective of kdr allele frequencies, and laboratory bioassays showed no increase in organophosphate resistance levels. One of the alternative chemicals, diafenthiuron, applied once per year over two years, successfully suppressed mite numbers, whereas other treatments did not control mites. These findings demonstrate that different strategies are required to manage H. destructor with pyrethroid resistance and organophosphate resistance.

Empowering Australian insecticide resistance research with genetic information: the road ahead

AbstractInsecticides are important for chemical control of arthropod pests in agricultural systems but select for resistance as an adaptive trait. Identifying the genetic mechanism(s) underpinning resistance can facilitate development of genetic markers, which can be used in monitoring programs. Moreover, understanding of genetic mechanisms in a broader population genetic context can be used to infer the origins of resistance, predict the dynamics of resistance evolution and evaluate the efficacy of different management strategies. Transitioning genetic information successfully into practical solutions requires overcoming two major hurdles. Firstly, genetic mechanisms must be identified to develop genetic markers. Secondly, routine use of genetic markers is required to build substantial spatio‐temporal data on the distribution and frequency of resistance alleles. In this study, we demonstrate large knowledge gaps on the genetic mechanisms of insecticide resistances in Australia using eight established arthropod pests important to the grains industry: Bemisia tabaci (silverleaf whitefly), Frankliniella occidentalis (western flower thrips), Halotydeus destructor (redlegged earth mite), Helicoverpa armigera (cotton bollworm), Myzus persicae (green peach aphid), Plutella xylostella (diamondback moth), Tetranychus urticae (two‐spotted spider mite) and Thrips tabaci (onion thrips). Many resistances have not been characterised at the genetic level in most pests, even for chemical MoA groups with a long history of use in Australia. Moreover, monitoring of resistance is spatio‐temporally patchy, which precludes examination of long‐term trends or predictive modelling. We suggest that leveraging cumulative global knowledge of resistances to develop a priori candidate genes, and incorporation of genomic approaches, can help overcome the hurdles of embracing genetic information in resistance management. We highlight the recently invasive Spodoptera frugiperda (fall armyworm) as a case study where genetic markers and genomic approaches should prove useful in rapidly assessing the risk of this species to the Australian grains industry and other agricultural commodities. The uptake of genetic information into management can only occur once its benefit to empower insecticide resistance research is fully realised. Ultimately, the road ahead requires amalgamation of multifaceted data (genes, environment and spatio‐temporal replication) to better understand and predict the dynamics of resistance evolution.

A method to investigate neonicotinoid resistance in mites.

The redlegged earth mite (Halotydeus destructor) is an important agricultural pest in Australia, with a wide range of plant hosts. Halotydeus destructor has developed resistance to pyrethroid and organophosphorus insecticides as a consequence of the widespread use of these chemicals by farmers. Neonicotinoids are one of the few remaining insecticide classes registered against H. destructor in which resistance has not been detected, although there have been occasional reports of control difficulties experienced in the field. There is currently no reliable way to accurately test the response of H. destructor (or indeed any mite species) to neonicotinoid insecticides. Here, we developed a new bioassay to assess the response of mites against the neonicotinoid imidacloprid. The method provided consistent results and showed no variation when used by different operators. We generated base-line sensitivity data for imidacloprid across a number of field-collected populations of H. destructor. This is important for future monitoring of mite responses given the considerable selection pressure now being exerted across large areas of the Australian farming landscape through the widespread use of neonicotinoid seed treatments.

Origin of resistance to pyrethroids in the redlegged earth mite (Halotydeus destructor) in Australia: repeated local evolution and migration.

Halotydeus destructor is a major pest of crops and pastures across southern parts of Australia. This invasive mite has been chemically controlled for over 50 years, but resistance to synthetic pyrethroids and organophosphates is developing. Understanding processes behind the emerging resistance is important for effective management efforts. We undertook a ddRAD pool-sequencing approach to analyse genome-wide single nucleotide polymorphism variation in H. destructor population samples at two scales: local resistance across a set of fields, and regional resistance across their Australian range, along with toxicology bioassays to screen for pyrethroid resistance. Spatial patterns of genomic variation and resistance at a local scale indicated that genetic similarity among samples was more closely correlated with distance along roads and fence-lines than with straight-line geographic distance. This pattern was particularly strong in resistant samples, which were also more related than susceptible samples, suggesting local spread of resistance within an area after it emerged. By contrast, regional data suggest resistance has emerged repeatedly within parts of Australia. Our de novo annotation of the H. destructor draft genome sequence and Bayesian analysis identified several candidate loci strongly associated with population-level resistance to pyrethroids, located in genomic regions that code for transmembrane transport and signalling proteins that have previously been linked to insecticide resistance in other arthropods. Our findings highlight multiple independent evolutionary events leading to resistance in H. destructor, and demonstrate the utility and cost-effectiveness of a cross-population, genome-wide association study to reveal processes underlying adaptive evolution in a non-model invasive species. © 2019 Society of Chemical Industry.

Pyrethroid resistance in the pest mite, Halotydeus destructor: Dominance patterns and a new method for resistance screening

An L1024F substitution in the para gene, which encodes a subunit of the voltage-gated sodium channel, has been implicated in pyrethroid resistance in a mite pest, Halotydeus destructor, which attacks rape and other grain crops. A high-resolution melt (HRM) genotyping assay was developed for testing the relative pyrethroid susceptibility of different para genotypes and for high-throughput field screening of resistant alleles. The L1024F mutation was found to be incompletely recessive in phenotypic laboratory bioassays with the pyrethroid pesticide, bifenthrin. While the resistance ratio of heterozygotes (RS) to susceptible homozygotes (SS) was <6 in 24 h bioassays, the resistant homozygotes (RR) (with a resistance ratio > 200,000) survived the recommended field rate of bifenthrin (100 mgL−1). HRM genotyping of mites from field populations across Australia indicated the presence of resistant alleles in Western Australia and South Australia, but not in Victoria and New South Wales. The assay developed will be useful for routine screening of pyrethroid resistance, and the dominance relationships established here point to useful resistance management strategies involving the maintenance of reservoirs of susceptible mites to dilute resistant homozygotes in a population.

Field margins provide a refuge for pest genes beneficial to resistance management

Fencelines and field margins in broad-acre cropping systems are commonly a refuge for weeds, diseases and invertebrates because they avoid many cropping and pest management regimes applied inside fields. As such, fenceline refuges are often managed separately with the goal to reduce pest reinfestation of fields from the margins. However, the implications of these pest control strategies are poorly understood in terms of their impact on pest genes beneficial to pesticide resistance management. Fenceline management, such as selectively reducing pest populations through insecticides, or non-selectively modifying habitat quality by removing host weeds with herbicides, might increase or decrease resistance evolution rates. Indeed, the potential to perform selective and non-selective control of pests separates management of field margins from structured in-field susceptible refuges (e.g. Bt crops). Here, a simulation approach was used to explore the effect of different fenceline management strategies, cropping characteristics and pest genetics on resistance evolution. The analysis was applied to a major crop pest, the mite Halotydeus destructor, for which fenceline treatments of herbicides and insecticides may be applied. Spraying fencelines with an insecticide decreased reinfestation and the overall abundance of mites, compared with not applying insecticides to fencelines. However, in all scenarios tested, resistance evolution was delayed by leaving fenceline refuges unsprayed with insecticides or herbicides. Just as field margins may provide a reservoir for invertebrates beneficial to pest management (e.g. predators and parasitoids), they may also serve as an important refuge for genes beneficial to resistance management.

Summer diapause intensity influenced by parental and offspring environmental conditions in the pest mite, Halotydeus destructor

The regulation of active and dormant stages of arthropods is critical for surviving unfavourable seasonal conditions, and for many species depends on the diapause intensity (DI). There is substantial information on diapause strategies of arthropods under winter conditions; however, most cases of summer diapause are poorly understood despite its importance in most geographic regions of the world. Here we show how complex interactions with the environment drive DI involving multiple summer diapause forms of the mite Halotydeus destructor. This invasive pest in Australia is only active in cooler months but enters diapause at the egg stage which can survive hot and dry summer conditions. Recent research points to two forms of diapause egg, a typical form with a thick chorion and a cryptic form without this chorion which is morphologically similar to non-diapause eggs. Compared with typical diapause eggs which are produced in late spring, cryptic diapause eggs could be produced together with non-diapause eggs earlier in the season with relatively cooler temperatures and shorter daylength, reflecting an advanced bet-hedging strategy. Fitness trade-offs in this strategy are investigated in this study as variability of DI of the typical and cryptic diapause forms under different environmental factors for incubating diapause eggs (temperature) and rearing parental mites (different daylength, temperature and soil moisture). With the exception of daylength, all factors impacted hatchability of diapause eggs. Higher mortality of cryptic diapause eggs indicated relatively shallower DI than typical diapause eggs, likely reflecting a fitness penalty of this bet-hedging strategy under some conditions. Hatchability of cryptic diapause eggs revealed thermal and moisture stresses have opposite and complementary effects between parental and filial generations. Although DI of filial eggs decreased in hot and dry summer conditions, parental mites reared in hotter and drier conditions increased the DI of offspring. A bet-hedging strategy involving cryptic diapause might be replaced by typical diapause under consistently stressful conditions because of higher survival, regardless of additional production costs that might be required. These findings highlight a complex set of plastic responses to summer conditions in H. destructor that undoubtedly contribute to the success of this invasive pest under a range of environments.

Field associations of first generation densities of the pest mites Halotydeus destructor and Penthaleus major in pasture.

Halotydeus destructor and Penthaleus major are species of earth mite commonly found at high densities in agricultural fields in Australia and other parts of the world. These mites pose a risk to a range of winter crops and pastures when seedlings emerge in autumn. In order to predict likely mite pressure, we investigated whether autumn densities in pastures can be determined from agronomic and environmental field variables. For H. destructor, field densities showed little association with a range of vegetation variables but could largely be explained using the variable field type, with high densities present when fields had mixtures of grass, clover and weeds. For P. major, we found a regional effect. In the region where most data were available, P. major field densities were associated with grass abundance, whereas an association with field type was significant but different to that found for H. destructor. For both species, densities were not associated with rainfall, but there was a weak association with soil moisture capacity. We discuss how these results can help in managing these important pest mites.

A cryptic diapause strategy in Halotydeus destructor (Tucker) (Trombidiformes: Penthaleidae) induced by multiple cues.

The polyphagous mite pest, Halotydeus destructor, typically has three generations during the cool moist season in Australia and produces over-summering diapause eggs in spring. Diapause eggs have a distinct thick and dark chorion and can survive heat, desiccation and the application of pesticides. Farmers suppress mites producing diapause eggs by a carefully timed spring pesticide application using Timerite® , which predicts the onset of diapause egg production based largely on day length. We investigated the association between diapause induction and other environmental factors that may complicate diapause predictions. Diapause in H. destructor induction was influenced by three interacting environmental factors, namely day length, temperature and soil moisture. A cryptic type of diapause egg that lacked a thick chorion and was instead morphologically similar to non-diapause eggs was also discovered. Like diapause eggs, this newly discovered egg type could also survive hot and dry summer conditions. There is an opportunity to refine the Timerite® spring spray by incorporating knowledge of other environmental factors inducing diapause in H. destructor. Compared with typical diapause eggs, the production of cryptic diapause eggs could reflect a strategy to deal with diversifying environmental stresses and/or represent a bet-hedging strategy to adapt to unpredictable environments. © 2018 Society of Chemical Industry.

Influence of previous host plants on the reproductive success of a polyphagous mite pest, Halotydeus destructor (Trombidiformes: Penthaleidae).

In the evolution of phytophagous arthropods, adaptation to a single type of host plant is generally assumed to lead to a reduction in fitness on other host plant types, resulting in increasing host specialization. While this process is normally considered to be genetically based, short-term effects acting within one generation (plasticity) or across two generations (cross-generation variation) could also play a role. Here, we test these effects in the redlegged earth mite, Halotydeus destructor (Tucker) (Prostigmata: Penthaleidae), a major agricultural pest of multiple crop plants. Field populations of mites were collected from grasses, legumes, and broad-leaf weeds and placed into enclosures with different plant types. The survival, net reproductive output (Ro), and feeding damage of each mite population were assessed across two generations. The interaction between the origin of mites and plant type had a significant effect on parental survival, Ro, offspring development, and feeding damage. Mites collected from legumes showed higher parental survival on all host types; however, Ro, offspring development and feeding damage were all higher when mites were placed onto the same plant type from which they were collected. These patterns point to the ability of H. destructor to perform well on host plants even in the absence of genetically differentiated host races, but also the likelihood of performance trade-offs when populations are forced to rapidly change hosts within and across sequential generations.