Microscopic Hair Research Articles

Species Identification of Inuit Skin and Fur Clothing: Analyses of DNA, Hair Microscopy, and Macroscopical Identification

From approximately 1830 to 1940, through various expeditions to Siberia, Arctic North America, and Greenland, and through donations, the National Museum of Denmark acquired its collection of historical Inuit skin clothing. Unfortunately, original provenance information has been lost for 14% of the garments. In order to document the extensive collection, this study investigates three methods for species identification of animal skin: microscopy of hair, macroscopic identification, and DNA validation. Thus, the present study has two aims: first, to optimise and test hair microscopy for species identification by validating identifications by DNA analyses, and second, to use species identification to estimate the geographic and cultural provenance of Inuit skin clothing. Based on a dataset of well-documented clothing (for positive controls), this study describes an optimised species identification protocol via hair microscopy using transmitted light microscopy (TLM). We demonstrate that the TLM hair protocol is a reliable and inexpensive alternative to molecular approaches when macroscopic identification is doubtful and DNA validation or protein analyses are impossible. In this study we used photomicrographs to document the identifications of caribou (Rangifer tarandus), musk ox (Ovibos moschatus), species of the true seal family (Phocidae), domestic dog (Canis lupus familiaris), wolf (Canis lupus), Arctic fox (Vulpes lagopus), polar bear (Ursus maritimus), wolverine (Gulo gulo), ground squirrel (Urocitellus parryii richardsonii), ermine (Mustela erminea), and cattle (Bos taurus); these identifications can be used for future reference. We conclude that species identification analyses secure the documentation of garments and allow most objects to be contextualized by culture and geography. The studied garments are accessible at the museum website.

Challenges Facing Us in DNA Analysis from Human Hair Samples: A Review

Human hair is a valuable tool in criminology, aiding in population studies through statistics and DNA analysis. It's easily identifiable at crime scenes, often clinging to clothing, carpets, and various surfaces, including animal fur, through a process known as secondary transfer. Forensic analysis of hair evidence serves several purposes; it can help establish the possibility of a connection between a suspect and a crime scene or between a suspect and a victim. It can demonstrate that there is no evidence linking the perpetrator to the crime scene or the suspect to the victim. While microscopic hair analysis cannot definitively identify a specific individual as the source of the hair, it does provide a solid basis for association. The wealth of macroscopic and microscopic details available in hair examination can also provide strong evidence for the defense. To understand the challenges of conducting DNA tests on hair, it's essential to study hair structure and composition. DNA in hair is not evenly distributed throughout every part of the hair; it exists in both nuclear and mitochondrial forms.

Protein Biomarkers for the Identification of Forensically Relevant Human Hair from Different Body Parts in Intimate Contact Cases.

Correctly identifying the human hair anatomic location found at crime scenes can link biological sample donors with the actual crime event, thus providing significant insight into the crime scene reconstruction. Forensic proteomic studies on human hairs can facilitate the development of new biomarkers for hair identification while compensating for the limitations of the conventional morphologic hair comparison and DNA analysis. Herein, the LC-MS/MS platform was used to find differentially expressed protein biomarkers in hairs from different body sites. The findings indicated that a total of 296 protein biomarkers with statistically significant differences in body sites were initially identified, and hair samples from the scalp, pubic, and armpit parts were distinguished from each other, which were validated by multiple bioinformatic methods. Fewer differences in protein patterns between armpit and pubic hairs while larger differences between hair and armpit as well as pubic hairs provided reasonable evidence of sexual or close intimate contact in crimes. This study lays the foundation for the development of a more reliable strategy to distinguish human hairs of various body areas from Chinese and will also support microscopic hair comparison analysis and assist in the proper handling of legal proceedings in relative cases by judicial officers, deserving special attention and further in-depth investigation. The MS proteomics data have been deposited to the ProteomeXchange Consortium via the iProX partner repository with the dataset identifier PXD038173.

Scalp hair loss is not random across follicular units: A new insight into human hair ageing.

Scalp hair has the greatest number of hairs (typically 1-5) per follicular unit but is also the most susceptible body site to hair loss with age. Hence, we set-out to determine the degree to which scalp hair parameters change with age in women and men, any sex differences thereof and whether hair loss is random across follicular units. A retrospective cross-sectional study of 200 Chinese men and 200 Chinese women (30-69 years). Image analysis and manual counting methods were used to measure occipital located hair parameters from 6 × 8 mm shaved scalp photographs and plucked hair microscopy images. Of the five hair parameters, the number of hairs per follicular unit had the greatest (negative) correlation with age in both men and women. Men had a greater number of hairs and follicular units than women on average but had a greater decrease in the number of hairs per follicular unit with age, particularly for the loss of multi-hair (3+) follicular units. The loss of hairs with age was significantly different to that expected by a random loss of hairs across follicular units and better described by a model of increased hair loss risk the greater number of hairs per follicular unit. We have found evidence of hair loss preferentially occurring in multi-hair follicular units, which was more pronounced in men. These data suggest that part of the reason scalp hair is more susceptible to hair loss than on other body sites is due to the greater presence of multi-hair follicular units on the scalp.

Raman Spectroscopy and STR Analysis of the Elongated Skulls from the Paracas Mummies of Peru

Between 1880-1947, several hundred mummified bodies were excavated from burial sites in the Paracas peninsula of Peru. Each set of recovered remains displayed reduced stature and severe cranial elongation, which led many anthropologists and archaeologists to question the genetic origins of what would be colloquially referred to as the “Cone Head mummies.” Since the discovery of these bodies, suggestions have been made that the mummies’ cranial deformations point to another human species, an unknown hominid species, or even within some groups, an alien. This project sought to employ commonly used forensic techniques, including but not limited to Raman spectroscopy and DNA fingerprinting, to investigate the hair structure and genetic profile from tissue and hair samples derived from the Paracas skulls. Tissue and hair samples were subjected to comparative hair analysis, Raman spectroscopy, and DNA typing using short tandem repeat (STR) analysis. Comparative microscopic hair analysis and Raman spectroscopy of samples collected from the Paracas skull as well as from humans of varying ethnicities revealed very similar hair structures and characteristics that are associated with human hairs. Following DNA extraction and amplification of tissue samples, STR typing results demonstrated allelic profiles similar and consistent with those DNA profiles observed in modern human populations. Moreover, no foreign DNA or unusual patterns/profiles were observed in any of the samples tested. Data generated to this point strongly suggest that the Paracas mummies are of the same species as humans and not from an unknown hominid or alien society.

Species Identification of Common Native Arctic Mammals in Inuit Fur Clothing Based on Hair Microscopy

ABSTRACT Correct material identification is considered essential when documenting museum objects. This study examines the morphology of mammal hair and records the geographical use of common species in Inuit fur clothing collected by the National Museum of Denmark (NMD) from c. 1830–1940 in the Bering Strait region, Alaska, Arctic Canada, and Greenland. Through hair microscopy, the purpose is to test whether original identifications are correct to assess the origin of unique Inuit garments. By means of transmitted light microscopy (TLM) of stained, 1 µm thick cross-sectioned hairs and undyed, longitudinally mounted hairs, the research reveals that specific morphological structures are characteristic of the common native reindeer/caribou, musk ox, members of the seal family, domestic dog, wolf, Arctic fox, polar bear, and wolverine. Rarer animals (hare, lynx, otter, etc.) are not part of this study because of limitations in the collection. Hairs from seal species are difficult to distinguish from one another. Hairs from dog and wolf are distinguishable but have relatively similar morphology. Therefore, to confirm identification, supplementary analyses are required. The hair microscopy technique was used on 49 garments in NMD’s collections, and the results were compared to the original macroscopic species identification. The study revealed that the latter method is often erroneous when it comes to dog/wolf and wolverine fur.

P229 Three cases of easy to be misdiagnosed tinea of vellus hair

Poster session 2, September 22, 2022, 12:30 PM - 1:30 PM Vellus hair, also known as fine hair, is generally accepted that tinea of vellus hair is caused by dermatophyte infection of the vellus hair and fungal microscopy reveals the presence of mycelium and/or spores inside and outside the vellus hair. Different types of dermatophytes can cause different types of lesions. Therefore, tinea of vellus hair is easy to underdiagnose or misdiagnose in clinical practice. We report three cases of tinea of vellus hair caused by Microsporum canis. Case 1: A 24-year-old male patient with bilateral erythema with pruritus on the upper extremities for more than 1 year (A1). The patient was diagnosed with tinea of vellus hair by positive dermatoscopy (A2), fungal fluoroscopy (A3), and fungal culture of M. canis. after 9 weeks of treatment (oral itraconazole 0.2 g, 2 times/day, topical amorolfine cream 1 time/day, terbinafine cream 1 time/day), the skin of bilateral forearms basically returned to normal, and it is currently under follow-up. Case 2: A 5-year-old female patient with a rash on the root of the nose for 2 weeks (B1). She was also diagnosed with tinea of vellus hair by positive dermatoscopy (B2) and fungal fluoroscopy (B3) and fungal culture of M. canis. After 2 weeks of treatment (oral itraconazole 0.1 g, 2 times/day, topical terbinafine cream once a day), the lesions improved significantly. After 4 weeks of treatment, a few scales and scabs were still visible on the skin at the root of the nose. After 2 weeks of continued treatment, the patient's skin at the root of the nose was completely restored to normal. Case 3: 2-year-old male patient with a recurrent facial rash with scales for more than 3 months (C1). He was diagnosed with tinea of vellus hair by positive dermatoscopy (C2), fungal fluoroscopy(C3), and fungal culture of M. canis. After 2 weeks of treatment (oral itraconazole 0.1 g once every other day, bifonazole cream 1 time/d), the lesions are in improvement from the previous. And this case is still being followed up. The above three cases have been misdiagnosed as tinea simplex or eczema, and the efficacy of treatment only with topical medicines or short-term oral drugs is not good. Therefore, we take oral medicines and topical medicines for treatment, the course of treatment is long, and there are still spores and hyphae inside and outside the hair many times during the treatment period, so it is recommended that tinea of vellus hair should be treated according to the treatment plan of tinea capitis.

Solving the Riddle of Developmental Delay with Hair Microscopy: Trichothiodystrophy (MPLKIP Mutation in an Indian Child).

Solving the Riddle of Developmental Delay with Hair Microscopy: Trichothiodystrophy (MPLKIP Mutation in an Indian Child).

Characterization by Raman and infrared spectroscopy and fluorescence microscopy of human hair treated with cosmetic products

Analytical studies on hair structures have evolved significantly over the years and vibrational spectroscopic techniques, such as Raman and infrared, have been increasingly used for such purposes. Nowadays, there is a need to understand more and more about the action of cosmetics on the hair fiber, so this work aims to analyze the permeation of cosmetic treatments into the hair. For the molecular structural characterization, Raman and infrared spectroscopy techniques were used, being verified the efficiency in the analysis of hair samples, demonstrating the internal characteristics of the fiber and the permeation of different cosmetics. Four cosmetics were chosen for this study and, due to the techniques used, it was possible to observe the diffusion of these products inside the bleached hair. It was observed with the Raman vibrational spectroscopy that the concentration of the products is found mainly in the cuticular region, decreasing the permeate content when approaching the central region, and the infrared spectroscopy showed results compatible with the Raman spectroscopy. Therefore, vibrational spectroscopy proved to be a valuable tool for the study of cosmetic permeation into the hair fiber and for the analysis of its external and internal structure.

微小な毛は生命と進化の立役者

微小な毛は生命と進化の立役者

Unraveling the mystery of confiscated "jackal horns" in India using wildlife forensic tools.

Internationally, illegal wildlife trade involves highly prized and charismatic species and their derivatives. At the same time, common or less known species and their parts are also encountered but receive less attention than charismatic species. Given the increasing demand for wildlife products in many parts of the world, profit, and short supply, many fake articles derived from domestic or wild animals are frequently encountered in the wildlife trade. Jackal horn (locally known as "Siyar or Gidar singhi") is one such fake item widely used in sorcery and other occult practices available through offline and online trading platforms within India. We used a combination of morphological, microscopic hair, and molecular approaches (Cyt b and 16s rRNA genes) to reveal the true identity of confiscated "jackal horns" (n = 342). Detailed morphological study of the jackal horns showed that it varied in size, shape, color of hair, attachment material, and filling material. The microscopic hair and molecular approaches revealed that all the items sold as jackal horns were fake and made up of protected wild species and domestic animals. Our results confirm the use of the biological samples from few wild species protected under the Wild Life (Protection) Act, 1972, of India. Therefore, the law enforcement agencies are cautioned to get forensic opinions while dealing with such counterfeit items.

Case series on Silvery Hair Syndromes: Single Center Experience.

Background:Silvery Hair Syndromes (SHS), an autosomal recessive inherited disorder, includes Chediak–Higashi syndrome (CHS), Griscelli syndrome (GS), Hermansky–Pudlak syndrome (HPS), and Elejalde syndrome. Associated immunological and neurological defects and predilection for hemophagocytic lymphohistiocytosis (HLH) makes them a distinctive entity in pediatric practice. Thorough clinical examination, bedside investigations such as peripheral blood smear (PBS) and hair microscopy, and bone marrow (BM) examination are inexpensive and reliable diagnostic tools.Methods:We report 12 cases with SHS (CHS, n = 06; GS, n = 04; HPS, n = 02).Results:8 out of 12 SHS children (CHS-05, GS-03) presented with HLH. Out of 5 cases of CHS with HLH, 2 died, 3rd is stable post-chemotherapy; 4th completed chemotherapy, underwent matched related hematopoietic stem cell transplant (HSCT), and is stable 8 months off treatment. The 5th child completed chemotherapy and is in process of transplant. One CHS child without HLH is thriving without any treatment. Of the 4 GS cases, 3 presented with HLH and received chemotherapy (HLH 2004 protocol). One lost follow-up after initial remission; another had recurrence 7 months off treatment and discontinued further treatment. The third child had recurrence 1.5 years after initial chemotherapy; HLH 2004 protocol was restarted followed by HSCT from matched sibling donor; is currently well, 2.5 years post-transplant. One child with GS had neurological features with no evidence of HLH and did not take treatment. Of 2 children with HPS, one presented with severe sepsis and the other with neurological problems. They were managed symptomatically.Conclusion:In SHS with HLH, chemotherapy followed by allogeneic hematopoietic stem cell transplantation is a promising curative option.

Evaluation of hair structural abnormalities in children with different neurological diseases

Hair microscopy is a fast and effortless diagnostic method for many diseases affecting hair in daily practice. Many diseases can present with hair shaft disorders in pediatric neurology practice. Children with pathological hair findings were included in our study. Microscopic evaluation of the hair was performed under light microscopy. The clinical findings, pathological hair shaft findings, laboratory tests, and final diagnosis of the patients were evaluated. In our study, 16 patients with rare pathological hair findings were identified. Of these 16 patients, nine were diagnosed with giant axonal neuropathy, three with Griscelli syndrome, two with Menkes disease, and two with autosomal recessive woolly hair disease. In hair inspection, curly and tangled hair in patients with giant axonal neuropathy; silvery blond hair in patients with Griscelli syndrome; sparse, coarse, and light-colored hair in patients with Menkes disease; and hypotrichosis in patients with autosomal recessive woolly hair were remarkable findings. Dystrophic hair was detected in most of the patients on light microscopy. In addition, signs of trichorrhexis nodosa, tricoptylosis, and pili torti were found. In particular, pigment deposition in the hair shaft of two patients diagnosed with Griscelli syndrome and pili torti findings in two patients with Menkes disease were the most important findings suggestingthe diagnosis. Detection of hair findings in the physical examination and performing light microscopic evaluation facilitates the diagnosis of rare diseases accompanied by hair findings. A hair examination should be performed as a part of physical and neurological examinationson eachpatient regardless of thecomplaint.

Feeding ecology of Indian leopard (Panthera pardusfusca) in the Hirekallugudda sloth bear sanctuary (Proposed sanctuary)in Arasikere, Karnataka, India

The feeding ecology of the Indian leopard (Panthera pardusfusca) was studied from February 2016 to September 2016 a total of 54 scat samples. These leopards feed on a variety of wild and domestic animals. Diet composition of the leopard will be primarily studied through scat analysis will be collected from well-defined sampling areas within the study period. A total of 12 prey species were successfully identified based on the microscopic hair analysis, percentage frequency of occurrence of prey hair, bones, or claws, in the scats and estimation of standard error using a regression equation and relative biomass consumed also recorded. However, the data regarding their diet composition is scanty and scat analysis revealed that the wild animals (45.00%) was their preferred diet, and domestic species including with dog and livestock were more frequently consumed (52.00%) while Sambar deer (23.70 %) and Goat (21.30 %) was most commonly used as diet during seasonal variations. Other species include porcupine, wild boar, hare, monkeys, peafowl, spurfowl, and mongoose some domestic species of sheep, cow, and some unknown species. Furthermore, the questioner’s survey is recorded for human-leopard conflicts with investigations to cattle watchers also assessed.

Scalp roof tiles.: A new diagnostic sign in neonates with Netherton Syndrome.

Clinical PresentationA 2-month-old baby boy, of full-term spontaneous vaginal delivery, presented to the dermatology outpatient clinic with generalized erythroderma, which had been noted since birth. Family history was positive for similar disease in his eldest sister, who died at 6 months of age without a diagnosis. On examination, the patient looked ill with generalized erythroderma, yet there were no signs of ectropion, eclabium, or deformed ears. Diffuse scalp scaling was observed with interlocking tessellation scales over the scalp (Figure 1). Hair microscopy showed in Figure 2. All laboratory results were normal.

Hair as a tool for identification of predators and prey: a study based on scats of jaguars (Panthera onca) and pumas (Puma concolor)

Abstract: Microscopic hair identification is a non-invasive, simple, and economical method applied in scientific studies to identify mammal species. In ecology, this method is used mainly in mastofaunistic inventories and dietary studies. In the last decade, the number of dietary studies using the microscopic identification of hairs has grown substantially, but the application of this technique as a tool for the identification of both predators and prey species is still scant. Thus, the aim of this study was to identify predator and prey hairs in scat samples from the two largest species of carnivores in the Neotropical region, the jaguar (Panthera onca Linnaeus, 1758) and the puma (Puma concolor Linnaeus, 1771). We examined a total of 100 scat samples being 50 from the Pantanal of Mato Grosso do Sul and 50 from the Atlantic Forest of Paraná. We used different identification categories that included the hair microscopic and macroscopic identification, as well as the use of hooves and nails present in the scats associated with tracks and kills found in the field. We identified 57 prey items in the Pantanal samples and 61 in the Atlantic Forest samples. Predator´s hairs were identified in 34% of Pantanal samples and in 46% of Atlantic Forest samples. The combination of hair microscopic and macroscopic characteristics was efficient in the identification of different taxonomic levels, with most identifications reaching the level of the species. However, the methodological protocol for microscopic hair identification was not fully effective in obtaining all the microstructural patterns of the studied mammals. Adjustments in the technique are necessary to differentiate microstructural characteristics of species belonging to the same family. We recommend macroscopic identification of scat content items (hairs, hooves or nails) of both prey and predators to be used to complete the microscopic hair identification technique in dietary ecological studies.

Differentiation of Morphologically Similar Human Head Hairs from Two Demographically Similar Individuals Using Amino Acid Ratios.

Human hair is frequently encountered as forensic evidence and can contribute valuable information to investigators. Conventional forensic hair analyses include microscopic hair comparison (MHC) and DNA analysis. However, MHC is not supported by statistics and DNA analysis cannot always be performed. Recent studies have demonstrated that evaluation of differences in the hair proteins may offer an alternate method to these analyses. In this study, an evaluation of the amino acids present in hair was investigated as an approach to differentiate morphologically indistinguishable hair samples from two demographically similar individuals. Proteins in the hair were digested using hydrochloric acid, and the resulting amino acids were derivatized with N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) for analysis using gas chromatography-mass spectrometry (GC-MS). Eight derivatized amino acids were detected and quantified relative to an internal standard, L-norvaline, and used to construct twenty-eight amino acid ratios. Hair samples were collected from four areas of the head on various days over the course of one month, and no significant differences in amino acid ratios (p-value > 0.05) were observed among the areas of the head, and the ratios were consistent over the time period of this study. Additionally, fifteen of these amino acid ratios were found to be significantly different between the two individuals when compared using a two-sample t-test (p-value ≤ 0.05). These data indicate that amino acid analysis was able to differentiate two morphologically similar hair samples from different individuals and demonstrates the applicability of this method to distinguish similar hair samples when DNA analysis cannot be performed.

The Dangers of Convictions Based on a Single Piece of Forensic Evidence

The overall goal of the criminal justice system is to ensure that perpetrators of crimes are duly punished and that victims of crimes are duly exonerated. As part of the effort to achieve this goal, the forensic disciplines have become very important in criminal investigations and prosecutions in identifying the guilty and in exonerating the innocent. There is a growing number of cases where people have been convicted based on a single piece of forensic evidence. However, some of the convictions have been found to be wrong, largely, due to the lack of adequate scientific validation of the forensic science methods. Some of these methods include latent fingerprint analysis, bite mark analysis, microscopic hair analysis and firearms identification. This article critically examines the application of forensic evidence in criminal prosecutions and highlights the dangers of convictions based on a single piece of forensic evidence. The findings of recent reports, such as, the National Academy of Sciences (NAS) 2009 Report and President's Council of Advisors on Science and Technology (PCAST) 2016 Report, confirm the critical role and broad scope of these forensic pattern-matching methods. The reports also indicate flaws that affect the accuracy of these methods, such as, inadequate scientific validation, coincidental results (erroneous match), human, laboratory and interpretive errors etc. Therefore, this article argues that the court should follow a cautionary approach when relying on a single piece of forensic evidence and that strong corroboration with other forms of evidence linking the accused to the crime should be required.

An international survey into the analysis and interpretation of microscopic hair evidence by forensic hair examiners

Due to inadequacies in historical hair examinations, the use and perceived value of microscopic hair evidence has reduced. Many reports have heavily criticised the use of pattern-based evidence including hair evidence as being unreliable, with police forces focussing on more individualising evidence. Hair evidence has been utilised in casework for many years due to its ability to transfer easily between individuals and crime scenes. Hair evidence can provide important information in criminal investigations, and in certain circumstances, aid in the identification of an individual, along with providing information to aid in the reconstruction of a crime scene. It has become apparent in recent studies, such as the 2012 FBI review, that hair evidence has been inappropriately utilised in case work and this, along with a preference for more discriminating methods such as DNA profiling, has led to both a lack of confidence in and reduction of use of hair evidence. The need for more standardised and objective methods is required along with improved methods for interpreting hair evidence. Since these reports, there has been no study investigating how these have affected the perceptions of hair evidence by forensic hair examiners or that has investigated what methods are being utilised to create a robust, objective and reliable interpretation of this evidence. This study aimed to assess the current similarities and differences in microscopic hair examinations and perceptions of its evidential value between countries, with a focus on the analysis and interpretation methods used by case work examiners and researchers.This study will discuss the findings of a survey and interviews completed by hair examiners. Previous surveys have been conducted by Aitken & Robertson (1986) and Murphy (2013), however the current international status of microscopic hair examinations is unknown.This study gathered responses across 9 countries. From this, it was identified that the microscopic examination of hair evidence is still valued highly by those practicing it. The amount of cases that an examiner has worked on that involve the examination of microscopic hair evidence is correlated to the perceived evidential value of hair evidence and to the value of morphological characteristics of hair. There are inconsistencies between the approaches used in interpreting this type of evidence. Subsequently, this study has generated an international understanding of the current status of hair examinations which was not previously known from an operational perspective.

ПРОБЛЕМА ЭФФЕКТИВНОСТИ МОРФОЛОГИЧЕСКОГО ИССЛЕДОВАНИЯ ВОЛОС ЧЕЛОВЕКА ПРИ ВЫПОЛНЕНИИ СУДЕБНО-БИОЛОГИЧЕСКОЙ ЭКСПЕРТИЗЫ

ПРОБЛЕМА ЭФФЕКТИВНОСТИ МОРФОЛОГИЧЕСКОГО ИССЛЕДОВАНИЯ ВОЛОС ЧЕЛОВЕКА ПРИ ВЫПОЛНЕНИИ СУДЕБНО-БИОЛОГИЧЕСКОЙ ЭКСПЕРТИЗЫ