Hair Dye Samples Research Articles

Determination and Assessment of Ammonia and Some Heavy Metals in Some Hair Dye Samples

This study aimed to measure the levels of ammonia and some toxic metals in different hair dye products at local markets of El-Beida City (Libya). Six types were bought from diverse shops at different locations from different companies and several brands in El-Beida markets. The concentrations of ammonia were ranged between (2.493-5.868µg/g) where the high content recorded in sample S5 (Beauty), while, the lowest content recorded in sample S4 (Luxe). The level of heavy metals was analyzed (zinc, copper, lead, and cadmium). The selected toxic of heavy metals concentrations were determined by flame atomic absorption spectrophotometer. The concentrations of the heavy metals in the samples ranged (0.09-1.30 µg/g) from zinc, (0.18-1.14 µg/g) from copper, (0.07-0.18 µg/g) from lead and (0.012-0.13 µg/g) from cadmium. The results were compared with the values of which found on the boxes of the hair dye products. The results were showed some differences between the calculated concentrations and the values recorded on the boxes of hair dyes. These heavy metals were strictly forbidden to be used as material (EU Directive No 1223/2009) for the production of cosmetic products, including hair dye.

Quantifying lead, copper, iron and arsenic in hair dye samples.

Heavy metals are very hazardous to human health. In the USA, the Food and Drug Administration (FDA) regulates cosmetics so that they are safe for consumer use. However, internet shopping has made it easier for non-FDA approved cosmetics to come into the United States. People have reported serious health problems after using counterfeit cosmetics purchased online. This project focuses on analyzing commercial hair dyes for the presence of As, Cu, Fe, and Pb. Dyes were purchased locally and online, digested in aqua regia and tested with a Shimadzu AA-7000 flame atomic absorption instrument. The concentrations of Cu, Fe and Pb were below the instrument detection limits of 0.02, 0.06, 0.4 ppm respectively, and the amount of As in one sample was inconclusive due to quality control failure. Overall, no significant amounts of metals were detected in the samples. This work was made possible by NASA West Virginia Space Grant Consortium Grant #80NSSC20M0055, and Fairmont State University Chemistry Program.

Determination of Some Trace Elements in Hair Dye Samples Available in Libyan Markets by Induction Plasma Emission Spectroscopy

The research aims to determine the concentrations of trace elements in some hair dyes imported and locally manufactured in Libyan markets. Collected 23 samples of henna and hair dyes from 11 brands, 6 brands of chemical hair dyes consisting of 3 colors, 1 brand of hair bleach, and 4 brands of henna dyes; we applied the standard digestion method, weighed 1g of each sample of hair dye and 0.5g of each model of henna, then mixed the piece with high purity nitric acid 69.5% and concentrated hydroxide acid 30% and put it on a hot plate until getting digested and after that were filtration them and Samples were dilute with deionized water. The concentration of iron, lead, cobalt, cadmium, chromium, tin, magnesium, potassium, and arsenic was measured using inductively coupled plasma optical emission spectrometry (ICP-OES). The results showed concentration of the trace elements in the samples ranged (<0.001-0.07ppm) from chromium, (0.53-95.9ppm) from potassium, (71.4-0.09ppm) from magnesium, (62.2-0.07ppm) from iron, (1.28-0.15ppm) from stannium. While the concentrations of the remaining elements of lead, cadmium, arsenic, and cobalt, the results were less than the detection limit. As for the physical analyses found that some values exceeded the standard limit.

An Electroanalytical Solution for the Determination of Pb2+ in Progressive Hair Dyes Using the Cork-Graphite Sensor.

Lead is one of the most toxic metals for living organisms: once absorbed by soft tissues, it is capable of triggering various pathologies, subsequently bioaccumulating in the bones. In consideration of this, its detection and quantification in products for human consumption and use is of great interest, especially if the procedure can be carried out in an easy, reproducible and economical way. This work presents the results of the electroanalytical determination of lead in three different commercial products used as progressive hair dyes. Analyses were performed by cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV) using a composite cork–graphite sensor in 0.5M H2SO4 solution or 0.1M acetate buffer (pH 4.5), in the presence and absence of hair dye samples. The H2SO4 solution gave better results in terms of analyte sensitivity than the acetate buffer electrolyte. In both cases, well-defined signals for lead were obtained by DPSV analyses, enabling the calibration curve and figures of merit to be determined. The limits of detection (LOD) were found to be approximately 1.06 µM and 1.26 µM in H2SO4 and acetate buffer, respectively. The DPSV standard addition method was successfully applied to quantify the lead in hair dye samples, yielding values below 0.45% in Pb. All three analyzed samples were shown to comply with the limit set by the Brazilian Health Regulatory Agency, i.e., 0.6% lead in this type of product. The comparison of the electroanalytical results with those obtained by the reference method, based on the use of inductively coupled plasma optical emission spectrometry (ICP–OES), confirmed that the electroanalytical detection approach is potentially applicable as a strategy for quality control.

Natural catalyst for luminol chemiluminescence: application to validate peroxide levels in commercial hair dyes.

Here, we report a hydrothermally treated green leaves (Moringa oleifera) extract exploited as an efficient and highly sensitive catalyst to catalyze the chemiluminescence (CL) reaction of luminol. In the absence of enhancer, this green and hydrothermally treated catalyst was found to significantly enhance the CL intensity ~3.5-fold compared with the traditionally used K3 Fe(CN)6 catalyst. The structure and surface morphology of the catalyst was elucidated using X-ray photoelectron spectroscopy, scanning electron microscopy, X-ray diffraction, and Raman spectroscopy. The synergistic effect of the catalyst in the CL reaction was systematically investigated in the presence of hydrogen peroxide using ultraviolet-visible and CL spectroscopy. Studies showed that the sensitivity of the catalyst could be amplified by adjusting several parameters such as pH of the medium and concentrations of the base and luminol. The sensitivity of the novel-type catalyst was examined through the validation of hydrogen peroxide levels in commercial hair dye samples. Markedly, the catalyst displayed ultrasensitivity to hydrogen peroxide as the limit of detection of hydrogen peroxide using this catalyst was determined to be 0.02 μM under optimized conditions. In general, the proposed inexpensive, ecofriendly, and nontoxic catalyst could enable the determination of hydrogen peroxide for diverse analytical applications.

Use of carbon black based electrode as sensor for solid-state electrochemical studies and voltammetric determination of solid residues of lead

For the first time carbon black based electrode modified with paraffin was applied as a sensor on voltammetry of immobilized microparticles (VIMP) approach for determination of lead solid residues in hair dye samples. The solid microparticles of Pb(II) (Pb(CH3COO)2(s)) immobilized into the carbon paste sensor containing carbon black and paraffin were firstly reduced at initial potentials and further reoxidized at around −0.60 V during anodic scan. Electroanalytical parameters as well as supporting electrolyte composition, and pH were also evaluated. An analytical curve in 0.2 mol L−1 phosphate buffer solution (pH 5.0) from 0.04 to 3.2 μg (R2 = 0.999) with detection and quantification limits of 4 and 13 ng, respectively, were achieved. The method was applied to quantify lead solid residues in hair dye samples without previous mineralization or complex sample pre-treatment. Besides adequate repeatability, stability and selectivity of the developed sensor based on VIMP features, the method using carbon black based sensor was considered advantageous comparing to the results recorded by a spectrometric method (relative error lower than 8%) from several analytical viewpoints.

Determination of Heavy Metals in Hair Dye Sale in Iranian Market: Dermal Sensitivity and Carcinogenicity Assessment.

Despite the high consumption of cosmetics especially hair dyes in Iran, limited studies have measured human exposure to heavy metals in these products. Therefore, due to the sensitivity of the issue from a health perspective, this study was performed for the purpose of dermal risk assessment of heavy metals in different brands of hair dyes in Kashan region. The present study is an analytical-observational study. In this study, 36 samples of hair dyes from four best-selling brands in Kashan market (Iran) were selected; then the concentration of lead, cadmium, nickel, chrome, and cobalt was measured by inductively coupled plasma optical emission spectrometry (ICP-OES) and reported as μg g-1 of dry weight; and finally, a health risk assessment was performed. The heavy metal concentrations in the hair dye samples were 1.825, 0.525, 48.15, 2.975, and 0.575μgg-1 for Pb, Cd, Cr, Ni, and Co respectively that were lower than the permissible limit. Both of HI and LCR were below of 1 and 10-6 that indicated heavy metal in this study had not probable non-carcinogenic and carcinogenic risks for the consumers of these products. An exposure-based sensitization Quantitative Risk Assessment determined an absence of skin-sensitizing potential. However, Cr, Ni, and Co were found at concentrations above the allergenic limit of 5μgg-1. According to the results, although due to low concentrations of toxic metals, there is a low level of exposure to heavy metals in hair dye users, but can cause potential health damage to users.

Highly selective and sensitive fluorescence determination of m-Phenylenediamine

This work presented a new fluorescence method for the determination of m-phenylenediamine (MPD) based on the reaction between MPD and acetylacetone. The formed compound Hacac-MPD exhibited strong blue fluorescence emission under 365 nm excitation. Its fluorescence intensity had an excellent correlation with the MPD concentration Under the optimal conditions, the developed method gave a linear range of 10–600 μg L−1 with a low detection limit of 4 μg L−1. Furthermore, the coexistence of common inorganic ions and other aromatic amines had hardly influence on the determination of MPD, demonstrating high selectivity. Additionally, the MPD in spiked water samples and hair dye samples was successfully detected by the developed method, and the recoveries were 97.5%-101.6% and 98.3%-98.9%, respectively. The established method exhibited a good application prospect for the determination of MPD in environment and daily necessities.

Biochar obtained from spent coffee grounds: Evaluation of adsorption properties and its application in a voltammetric sensor for lead (II) ions

Biochar is a carbonaceous material obtained by controlled pyrolysis of biomass in a low amount of oxygen. This material has functional chemical groups on its surface, which can interact with organic and inorganic compounds. In the present work, biochar samples were prepared from spent coffee grounds using pyrolysis temperatures between 200 and 700 °C. Biochar yielded at different conditions was activated by a chemical treatment using nitric acid (HNO3) as an oxidizing agent to increase surface groups. The characterizations of the pristine and chemically treated biochar samples showed a rise in functional groups, confirmed by FTIR with the appearance of new and intense carboxylic group bands. The variation of ID/IG ratio, calculated from the obtained Raman spectra, indicates an increase in defects and insertion of heteroatoms on the material surface after chemical treatment. Biochar samples were used as modifiers to prepare the carbon paste electrode (CPME) and evaluated lead ions' pre-concentration. The electroanalytical strategy was based on differential pulse adsorptive anodic stripping voltammetry (DPAdSV), performing the pre-concentration step under open circuit potential condition separately from the electrochemical cell. It was verified for samples submitted to chemical acid treatment, and, among them, the CPME prepared with the 300 °C pyrolyzed biochar showed the best response. Under optimized conditions, it was observed a linear dynamic range (LDR) from 0.128 to 2.44 µmol L−1, limits of detection (LOD) and quantification (LOQ) of 4.5 nmol L−1 and 15 nmol L−1, respectively. The effect of foreign ions on voltammetric response has been evaluated, and the proposed sensor was applied for the determination of lead ions present in gunshot residues and hair dye samples.

Determination of PPD in Hair Dyes Collected from Local Markets in El-Bieda City - Libya

This study aims to determine the para-phenylenediamine (PPD) in hair dyes by High-Performance Liquid Chromatography (HPLC). In the presented study, ten hair dye samples were collected from local markets in El-Bieda - Libya. A rapid, simple and reliable method is developed and validated for the determination of PPD in hair dyes samples using 50% methanol solution as solvent. The method was validated over a wide linear range of 5 – 25 µg/mL with correlation coefficients being consistently greater than 0.997. The LOD and LOQ were 1.21 µg/ ml and 3.67 µg/ ml, respectively. Relative standard deviation (% R.S.D.) ranged between 0.07 and 1.15 %. The minimum PPD level was observed in Beauty Touch (Blonde) sample (0.0855 % w/w), while Jourin sense Cosmetics (Black Blue) sample showed the highest PPD content (2.2526 % w/w). The HPLC measurments indicated that the results of PPD concentrations in Black colour samples were in the range of 0.3705 % - 2.2526 % w/w. Whereas its concentrations in anthor colour samples were in the range of 0.0855 % - 0.5936 % w/w. The level of PPD in Black colour samples were higher than other colour samples. The PPD content in all the samples analyzed in this study are well below the allowable limits set by the US Food and Drugs Administration.

Heavy Metals Determination in Hair Dye Samples Selected from Iraqi Markets

Heavy Metals Determination in Hair Dye Samples Selected from Iraqi Markets

222Rn and 238U concentrations in some samples of hair dyes in local Iraqi pharmacies

The presence of Radon and Uranium in any material is an important indicator especially for dangerous hair dyes. Since hair dyes are widely used and there are no database found for radiation concentrations on it. The research is concerned with the concentration of radon and uranium in 20 samples of hair colors that are available in the Iraqi markets. Radon and uranium concentrations are measured by nuclear track detector LR-115 Type-II. The results show that the concentration of radon in samples under study ranged between (40.39±0.98)Bq/m3 to (182.80±16.09) Bq/m3with an average of (111.57±8.95) Bq/m3. Regarding the Uranium concentration, it was found to range between (8.71±0.21) ppm to (33.12±2.09) ppm with an average of (21.26±1.46) ppm. In addition, the present results showed that there is a strong correlation (correlation coefficient= 0.805) between Radon and Uranium concentrations. Most Radon and Uranium concentrations in hair dye samples were lower than the maximum permissible levels recommended by the previous studies.

Trace determination of lead in lipsticks and hair dyes using microwave-assisted dispersive liquid-liquid microextraction and graphite furnace atomic absorption spectrometry.

A novel microwave-assisted dispersive liquid-liquid microextraction (MADLLME) technique according to the solidification of a floating organic droplet (SFO) and graphite furnace atomic absorption spectrometry (GFAAS) used for the extraction and determination of lead ions in lipsticks and hair dyes made in different countries. Lipstick and hair dye samples of different brands and colours were collected from local market in Kermanshah, Iran. After sample treatment with microwave-assisted acid digestion, an appropriate mixture of acetone, 1-undecanol and diethyl dithiophosphoric acid was injected rapidly into the aqueous sample containing lead ions, and as a result, cloudy mixture was formed. After centrifugation, the test tube was cooled for few minutes. The solidified 1-undecanol on top of the solution was transferred into a suitable vial and injected into the analytical instrument. Under the optimum experimental conditions (extraction solvent: 30μL of 1-undecanol; disperser solvent: 500μL of acetone; ligand concentration: 0.15% (v/v); pH: ~1.5 and without salt added), the enhancement factor of 96 was obtained. The calibration graphs were linear in the range of 0.3-50μgkg(-1) with a correlation coefficient (r(2) ) more than 0.995. The detection limit was 0.1μgkg(-1) . Consequently, the developed method was successfully applied to extract and determine lead ions in the lipsticks and hair dyes, and favourable results were obtained. The proposed method which applied in cosmetics showed excellent relative recoveries (90-109.7%) with relative standard deviations <8.3% (n=3) for all samples. The study revealed that the concentration of lead found in lipsticks and hair dyes on the Kermanshah market is far below the recommended limits as applied in Germany (20mg kg(-1) ) and Canada (10mg kg(-1) ) and confirmed that very low levels of lead are technically available in the final cosmetic products.

Invited: Metal-Semiconductor-Metal Photocurrent Chip for Hydrogen Peroxide and Biomolecular Sensing with Chemiluminescence

Photodetectors are generally the most promising, representative and widely-used type of detectors, and the applications of photodetectors are quite extensively[1-3]. Basically photodetectors are made of photosensitive elements which are able to turn the light signal into electrical signal. Nowadays, the detecting wavelength region is very broad and can be classified into infrared, visible light and ultraviolet zone; furthermore, the applications of each region are manifold especially in visible light region. Additionally owing to the nature of fast responding speed, it promises photodetectors an advantageous future in biomedical sensing territory[4-5]. This study is focus on fabrication method of the flexible[6-8] photodetectors and application to biomolecule sensing through luminescence. The photosensitive element is based on single crystalline silicon semiconductor material which is treated with chemical etching process to achieve pliable characteristic[9]. Then, the metal-semiconductor-metal[10-14]structural flexible photodetectors are achieved after integrated with simple fabricating processes. In detail, the accomplished devices are handled with immobilization steps to fix the target enzyme which is able to catalyze the luminescent reaction (Fig.1). Owing to only extremely stable sensor for biomedical use, it is necessary to ensure the operation function at any crucial conditions. In this study, we provide a portable biomolecule sensor with outstanding flexible characteristic and high sensitivity and it exhibits illustrious stable consequence in various situations. Figure 1.Schematic diagram of immobilization process flow and luminescence detection.A pliable silicon chip measured under fifty micrometers in thickness is achieved through a simple method via alkaline etchant. The silicon chip with designed thickness is featured of great flexibility and relatively little fragility. A surface modification is applied to the flexible silicon chip with non-toxic and easy-arranged texturing solution from texturization process. Owing to anisotropic etching, the three-dimensional structure of pyramidal shape is constructed onto the chip surface. The reflectivity decreases advantageously in virtue of increasing roughness, and the final reflectivity is only one-fourth in comparison with original smooth chip surface due to formation of surface microstructure. The flexible MSM photodetectors are accomplished after mantling metal electrode with well-designed pattern. The semiconductor material we used is featured of high natural abundance and the manufacturing processes are considerably uncomplicated.In taking advantage of immobilization technique, the targeting enzyme is bound to photodetector devices and as-prepared devices are employed in optical sensing of luminescence which is catalyzed by the immobilized enzyme. This optical sensor exhibits great flexibility and remarkable durability and shows marvelous stability even in various stretching conditions. Furthermore, the photodetector devices are featured portable and cost-efficient. A novel application of this detector in healthcare field which is a significant issue nowadays is performed on available hair dye products. The examination is taken into consideration owing to the potential damage caused by hydrogen peroxide of which the main ingredient in hair dye products (Fig.2). For this interdisciplinary-technology-based device, the flexible optical sensor can be utilized in multiple regions and it can be integrated with promising instruments in virtue of great mechanical property. Figure 2.Hydrogen peroxide detections for hair dye samples through luminescence sensing by flexible MSM photodetectors.

Detection of carcinogenic chromium in synthetic hair dyes using laser induced breakdown spectroscopy

A laser induced breakdown spectroscopic (LIBS) system, consisting of a pulsed 266 nm laser radiation, in conjunction with a high-resolution spectrograph, a gated intensified charge coupled device camera, and a built-in delay generator were used to develop a sensitive detector to quantify the concentration of toxic substances such as chromium in synthetic hair dyes available on the local market. The strong atomic transition line of chromium (Cr I) at 427.5 nm wavelength was used as a fingerprint wavelength to calibrate the detection system and also to quantify the levels of chromium in the hair dye samples. The limit of detection achieved by our LIBS detection system for chromium was 1.2 ppm, which enabled us to detect chromium concentration in the range of 5-11 ppm in the commercial hair dyes available on the local market. The concentrations of chromium in the hair dyes measured using our system were validated using a standard analytical technique such as inductively coupled plasma mass spectrometry (ICPMS), and acceptable agreement (nearly 8%) was found between the results obtained by the two methods (LIBS and ICPMS). This study is highly significant for human health, specifically for people using synthetic hair dyes for changing the color of their hair.

Simultaneous determination of 11 aminophenols in hair dyes by high performance liquid chromatography

An analytical method was developed for the simultaneous determination of 11 aminophenols in direct and oxidized hair dyes by high performance liquid chromatography (HPLC). The samples were extracted with methanol using sodium bisulfite for anti-oxidation. The purification was carried out with the high speed frozen centrifugation. The separation was performed on an Agilent Zorbax SB C18 column with the mobile phases of acetonitrile and an ion pair system of sodium heptanesulfonate and phosphate under a gradient elution. The analytes were detected at three different wavelengths of 230, 270 and 400 nm. In the concentration range of 0.05 - 500 mg/L, the correlation coefficients for the 11 aminophenols were not less than 0.9992. The limits of quantification including 4-amino-3-nitrophenol, 2-amino-5-nitrophenol, and 4-(2-hydroxyethyl) amino-3-nitrophenol were 5 mg/kg. Other aminophenols including 4-aminophenol, 4-methyl aminophenol, 3-aminophenol, 2-aminophenol, 5-amino-o-cresol, 4-amino-3-methylphenol, 5-(2-hydroxyethyl) amino-o-cresol and 2-amino-4-nitrophenol were 20 mg/kg. The recoveries of the aminophenols spiked at different levels ranged from 88.5% to 109. 5% with the relative standard deviations (RSDs) within the range of 2.2% - 8.3%. The commercially available hair dye samples were analyzed for the aminophenols and the results showed that the method was suitable for the determination of the 11 aminophenols in direct and oxidized hair dyes.

Application of graphene as the stationary phase for open-tubular capillary electrochromatography

We reported the application of graphene as a novel stationary phase in open-tubular capillary electrochromatography (OTCEC). The OT column was easily fabricated by electrostatic assembly of poly(diallydimethylammonium chloride) followed by self-adsorption of negatively charged graphene. The chromatographic properties of graphene-coated OT column were investigated by separation of nitroaniline isomers through variation of the buffer pH, sodium dodecyl sulfate concentration and separation voltage. The results demonstrate the application of graphene in OTCEC greatly improves the separation by largely enhancing the surface area thus increasing stationary/mobile phase interaction. The proposed OT column exhibits good repeatability with run-to-run RSD of retention time as low as 1.5% (n=5), and excellent stability for at least two-week usage with total ∼200runs. The method was successfully applied for analysis of nitroaniline isomers in hair dye samples.

Effect of ionic liquid on the determination of aromatic amines as contaminants in hair dyes by liquid chromatography coupled to electrochemical detection.

The room temperature ionic liquid (IL) 1-butyl-3-methylimidazolium bis-(trifluorometanesulfonyl)imide BMIm[NTf2] was used as a novel medium for improvement of separation and quantization of 16 aromatic amines typically present as contaminants in consumer products and detected by HPLC coupled to an electrochemical detector. The aromatic amines, namely 4,4'-diaminodiphenylmethane, 4-chloroaniline, 2-methoxy-5-methyl-aniline, 3,3'-dimethylbenzidine, 2,4-diaminotoluidine, 2-chloro-4-nitroaniline, 4,4'-oxydianiline, aniline, 3,3'-dichlorobenzidine, benzidine, 4-aminobiphenyl, o-dianisidine, o-anisidine, o-toluidine, 4,4'-methylene-bis-2-chloroaniline and 2-naphthyl-amine are oxidized in methanol/BMIm[NTf2] at a potential around +0.68V to +0.93V vs. Ag/AgCl at a glassy carbon electrode, which is the base for their determination by HPLC/ED. Using the optimized conditions of methanol/BMIm[NTf2] 70:30 (v/v) as mobile phase, flow-rate of 0.8 mL·min−1, column CLC-ODS, Eap = +1.0 V and T = 40 °C analytical curves were constructed for each of the tested amines. Good linearity was obtained in the concentration range of 1.09 mg·L−1 to 217 mg·L−1, with excellent correlation coefficients. The limits of detection reached 0.021 mg·L−1 to 0.246 mg·L−1 and good relative standard deviations (RSD, n = 3) were obtained from the measurements. Satisfactory recovery for each aromatic amine was achieved, ranging from 95 to 103%. The developed method was successfully applied to determine six aromatic amines present as contaminants in commercial hair dye samples.

Determination of nitroanilines in hair dye using polymer monolith microextraction coupled with HPLC

In this study, a novel method for the determination of nitroanilines in hair dye samples has been developed based on poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolith microextraction (PMME) and high-performance liquid chromatography (HPLC) analysis. Four nitroanilines, p-nitroaniline (PNAL), m-nitroaniline (MNAL), o-nitroaniline (ONAL), and 2,4-dinitroaniline (DNAL), are studied as representatives. To obtain optimum extraction efficiency, several experimental parameters including sample flow rate, sample volume, sample pH, and eluent flow rate have been investigated. Under the optimal experimental conditions, the linear regression coefficients of the standard curves are greater than 0.9990. The limits of detection for p-nitroaniline, m-nitroaniline, o-nitroaniline, and 2,4-dinitroaniline are 0.012, 0.008, 0.018, and 0.005 μg/mL, respectively. The intraday and interday relative standard deviations are less than 3.1 and 5.4%, respectively. The proposed method is simple, rapid, sensitive, and competent when used for the determination of nitroanilines in hair dye samples and the accuracy is assessed through recovery experiments.

QUANTITATIVE DETERMINATION OF COMMERCIAL OXIDATION HAIR DYES BY REVERSED-PHASE HPLC

A simple and reliable RP-HPLC method for the separation and quantitative determination of p-phenylenediamine, p-aminophenol, m-aminophenol, resorcinol, and 2,6-diaminopyridine in commercial oxidation hair dyes was developed. The separation was carried out on a μ-Bondapak C18 column with methanol-aqueous solution containing 0.1% triethylamine and 0.02 mol l−1 NH4Ac (pH = 5.20) (10:90, vol./vol.) as the mobile phase. Without a complex extraction procedure, the method is sufficiently rapid and accurate for routine analyses. Two kinds of commercial hair dye samples were determined successfully.