In today's society, the social or environmental problems that need to be solved and iteratively changed by people are becoming more and more complex. Along with the rising level of people's social life needs, people's demand for cultural life becomes more urgent, and museums become a brief habitat for people after work and study. The problems faced by people with disabilities in museums are not only about accessibility, but also about communication and interaction with other people and exhibits in the museum environment - the problem of barrier-free interaction. This study aims to explore the accessibility design of the Macao Museum, analyze the current situation of the hard and soft accessibility design of the Macao Museum, elaborate on the problems of the accessibility design of the Macao Museum, uphold the concept of humanized design, propose improvement strategies, and strive to help the disabled groups integrate into the public in their daily lives as much as possible through the humanized design of accessibility in all aspects.