The purpose of this research was to design and implement a flexible horizontal H-shaped microstrip antenna (flexible-HHMA) using various materials for cancer tumor detection. Microstrip antennas, which can also be utilized as tumor detection sensors and can function as both a receiver and a transmitter, are composed of two major components: the patch and the ground plane. The copper tape was used to analyze various materials’ impacts on antenna performance to form the patch and ground planes, and felt, jean, and photo paper was employed as the substrate. To accomplish this, antennas operating at frequencies ranging from 2 to 10 GHz, frequently used in biomedical applications, were constructed and computations performed using a full wave electromagnetic solver based on finite integration technique (FIT). The proposed microstrip antennas may be used as sensors in biomedical measurement systems that make use of the dielectric contrast between the healthy and malignant tissues at microwave frequencies.