Guangxi Zhuang Autonomous Region Research Articles

Six new species of Aspidistra (Asparagaceae: Convallarioideae) from China and Vietnam

This paper continues to publish new discovered from successive investigation of the species diversity of the Aspidistra genus in recent years. Six new species of Aspidistra (Asparagaceae) are described and illustrated: four from China, Aspidistra dentosa, A. dissecta, A. dongxingensis (SW Guangxi Zhuang Autonomous Region) and A. anlongensis (SW Guizhou Province), and two from northern Vietnam, A. longibracteata and A. backanensis. All described species are local endemics of southern China and northern Vietnam. Except for Aspidistra dongxingensis living in non-limestone areas, all other species are restricted to limestone regions. The morphology, ecology, phenology, distribution and photographs are provided for all novel taxa.

Analysis of Factors Affecting Fiscal Revenue in Guangxi Zhuang Autonomous Region

Analysis of Factors Affecting Fiscal Revenue in Guangxi Zhuang Autonomous Region

Three new species of the spider genus Meotipa Simon, 1895 from Guangxi Zhuang Autonomous Region, China (Araneae: Theridiidae)

Three new species of Meotipa Simon, 1895 are described based on specimens collected from Guangxi Zhuang Autonomous Region, China: Meotipa lingulata sp. nov. (female), M. pseudomultuma sp. nov. (both male and female) and M. tortuosa sp. nov. (both male and female). Detailed descriptions, photographs, line drawings, sequences of histone H3, and a distribution map of the three new species are provided.

Prediction of Suitable Regions for Danxiaorchis yangii Combined with Pollinators Based on the SDM Model.

Danxiaorchis yangii, a newly discovered fully mycoheterotrophic orchid. It relies on Lysimachia alfredii and Dufourea spp. for pollination, and environmental factors closely influence the growth and distribution of these pollinators, which in turn directly affects the growth and reproduction of D. yangii. Climate change threatens the suitable habitats for these three species, emphasizing the need to understand D. yangii's response. This study comprehensively utilized the field distribution of D. yangii and related climatic data, along with future climate predictions from global models, to predict the climate suitability areas of D. yangii under two greenhouse gas emission scenarios (SSP245 and SSP370) using species distribution models (SDMs), which encompassed a random forest (RF) model. Additionally, we selected the optimal ensemble model (OEM) for Dufourea spp. and applied generalized boosted models (GBMs) and RF for L. alfredii in our predictions. The study found that precipitation of the driest quarter plays a pivotal role in determining the distribution of D. yangii, with an optimal range of 159 to 730 mm being most conducive to its growth. Comparative analysis further indicated that precipitation exerts a greater influence on D. yangii than temperature. Historically, D. yangii has been predominantly distributed across Jiangxi, Hunan, Zhejiang, and the Guangxi Zhuang Autonomous Region, with Jiangxi Province containing the largest area of highly suitable habitat, and this distribution largely overlaps with the suitable regions of its pollinators.

Digital twin water conservancy early warning system based on physical level and numerical simulation in intelligent water conservancy construction

Abstract. In 2023, the Central Committee of the Communist Party of China and The State Council issued the Overall Layout Plan for the Construction of Digital China, emphasizing the construction of a smart water conservancy system with digital twin basins as the core. The Guangxi Zhuang Autonomous Region government has clearly proposed to actively build a "digital twin Pinglu Canal", and attaches great importance to the construction of digital twin platform application services during the construction of the Western land-Sea New Passage (Pinglu) canal. Aiming at the problems such as unsatisfactory early warning effect, inaccurate data and imprecise model of existing smart water conservancy technology, this paper proposes a digital twin system for disaster early warning based on physical level and numerical simulation technology, establishes a visual digital water conservancy model and digital twin smart water conservancy cloud platform, and combines multi-physics field and multi-dimensional coupling algorithm to respond to technical pain points. To achieve scientific and accurate monitoring and intelligent management, and contribute to the construction of digitally empowered Pinglu Canal.

The Effect of Green Entrepreneurship Education on Green Entrepreneurial Intentions: A Case Study of the Guangxi Zhuang Autonomous Region, China

This study integrates the concept of green entrepreneurship into the entrepreneurship education system, and grasps the correct way to promote the green entrepreneurship intention of college students. The model of promoting college students’ green entrepreneurship intention with policy support is constructed to answer the following research questions: what is the internal mechanism of green entrepreneurship education in promoting green entrepreneurship intention of college students in Guangxi, and how does green entrepreneurship policy affect its internal mechanism? Through a questionnaire survey of 304 college students from major cities in Guangxi, China, this paper investigates how college green entrepreneurship education affects college students’ green entrepreneurship intention through the desirability and feasibility of green entrepreneurship and actively explores the regulatory role of the institutional environment. The results show that the desirability and feasibility of green entrepreneurship play a mediating role between green entrepreneurship education and the promotion of college students’ green entrepreneurship intention, and the regulatory environment positively moderates the positive effect of desirability and feasibility of green entrepreneurship on green entrepreneurship intention. This study contributes to the literature on green entrepreneurship. On the one hand, by studying the mediating mechanism of green entrepreneurship desirability and feasibility, this study has added empirical evidence on the promotion effect of green entrepreneurship education on the green entrepreneurship intention of college students. On the other hand, this study emphasizes the impact of policy regulation on the strengthening process of college students’ green entrepreneurial intention. This study will help to build a characteristic and effective green entrepreneurship education system in colleges and universities, enhance college students’ green entrepreneurship intention and green core competitiveness, and drive them to enhance their sense of social responsibility and build “green development” values.

Methoxyflavone glucosides and caffeoyl phenylethanoid glycoside from Lysionotus pauciflorus: their structures and anti-ferroptosis activity.

Phytochemical investigation on the aerial parts of Lysionotus pauciflorus Maxim. (Gesneriaceae), a medicinal plant used in Guangxi Zhuang Autonomous Region, China, resulted in the isolation of 13 secondary metabolites including two methoxyflavones, six flavonoid glycosides, and five caffeoyl phenylethanoid glycosides. Among these, the chemical structures of previously undescribed metabolites (1-3) were elucidated to be nevadensin 7-O-β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranoside (1), nevadensin 7-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside (2), and 2-(3,4-dihydroxyphenyl)ethyl-1-O-β-D-apiofuranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 3)-β-D-(6'-O-E-caffeoyl)glucopyranoside (3) by detailed spectroscopic and HPLC analyses. Inhibitory activity of isolated compounds against RSL3-induced ferroptosis on human hepatoma Hep3B cells were evaluated.

Exploration and Research on the Development of Higher Vocational English Education in Guangxi

With the acceleration of economic development and globalization and the rapid development of China’s foreign economy, the position of English education in higher vocational education has become increasingly prominent. Particularly in the multi-ethnic and multi-cultural areas of Guangxi Zhuang Autonomous Region, higher vocational education undertakes the responsibility of talent export for the construction of the China-ASEAN foreign trade exchange platform. However, the current phenomena and problems existing in higher vocational English education in Guangxi need to be deeply discussed and solved. Therefore, this paper discusses the development ideas of English education mode in Guangxi higher vocational education and studies the feasibility suggestions, in order to provide a reference for the future development of higher vocational English education in Guangxi.

Brevibacterium litoralis sp. nov., a cellulose-degrading strain isolated from marine surface sediment.

A Gram stain-positive, non-spore-forming, non-motile, short-rod actinomyces strain GXQ1321T was isolated from maritime surface sediments in Beihai (21° 41' 21.65″ N, 109° 05' 76.56″ E), Guangxi Zhuang Autonomous Region, and a number of categorization studies were performed. Following a period of 72h of incubation at a temperature of 30°C within a modified actinomycete culture medium, the colony was light-yellow, circular, smooth, central bulge, convex, opaque, with a 1.2-2.3mm diameter. Strain GXQ1321T had the ability to degrade cellulose. Chemotaxonomic studies revealed that the major methylnaphthoquinones in strain GXQ1321T was MK-8(H2). The most prevalent cellular fatty acids were anteiso-C19:0, anteiso-C15:0, anteiso-C17:0, and iso-C16:0. The whole-cell sugars of the strain GXQ1321T were identified rhamnose, xylose and glucose. Meso-diaminopimelic acid was found in the peptidoglycan hydrolysate, and the polar lipids were identified as diphosphatidylglycerol, three phosphoglycolipid, phosphatidylglycerol and two unknown glycolipid. This strain had 69.6% DNA G+C content. Strain GXQ1321T is classified as Brevibacterium based on its 16S rRNA gene sequence. It is closely related to Brevibacterium samyangense SST-8T (96.8%) and Brevibacterium rongguiense 5221T (96.3%). The average nucleotide identity (ANI) values of GXQ1321T and the above two type strains were 73.9-77.1%, and the digital DNA-DNA hybridisation (dDDH) values were 15.3-21.1%. Based on the phylogenetic, chemotaxonomic and physiological data, strain GXQ1321T was considered to be a novel species of the genus Brevibacterium, named Brevibacterium litoralis sp. nov, with the type strain GXQ1321T (= MCCC 1K08964T = KCTC 59167T).

Hypodematium zhangii (hypodematiaceae) – a new species recorded from china

Hypodematium zhangii J. X. Li & X. J. Li, a new species of Hypodematium is recorded from Guangxi Zhuang Autonomous Region, China. It is similar to H. crenatum (Forssk.) Kuhn & Decken, but differs greatly from it by its stipe glabrous, rachis and fronds sparsely covered with hispidity adaxially, indusia and fronds densely coated with hispidity abaxially and spores perispore with tuberculate and massive protrusions, surface with scaly ornamentation. The description and photographs of H. zhangii as well as their notes are provided. Bangladesh J. Bot. 53(3): 795-802, 2024 (September) Special

The Emotional Impact of L1 Learning Confidence on L2 Learning: A Case of a Study of Translation Majors in Guangxi Universities

This study aims to explore the emotional impact of the confidence developed in Chinese learning on the foreign language learning state of translation major students in universities across Guangxi. A questionnaire survey was conducted with 132 translation major students from various universities in the Guangxi Zhuang Autonomous Region. The study found that the confidence developed during Chinese learning positively influences the learners' language learning, helping them view language learning more positively, fostering other positive emotions in the learning process, and encouraging more active engagement in bilingual interactive learning. This research fills a gap in the study of positive emotions in language learning, expands new perspectives for this research direction, and provides practical references for future studies.

Monitoring of Vegetation Drought Index in Laibin City Based on Landsat Multispectral Remote Sensing Data

Due to the impact of global warming, drought has caused serious damage to China’s ecological environment and social status. This article selects Laibin City in the Guangxi Zhuang Autonomous Region as the research area, utilizing multispectral remote sensing data as the data source and Landsat series image data for relevant preprocessing. It calculates the monthly normalized vegetation index (NDVI) and surface temperature (LST) data for Laibin City. Based on the ecological environment and surface coverage conditions of the research area, the ratio vegetation index (RVI), normalized vegetation moisture index (NDWI), temperature vegetation drought index (TVDI), and conditional vegetation temperature drought index (VTCI) were selected to calculate and invert the drought monitoring results of Laibin City. The drought monitoring results were obtained and overlaid with the vegetation area map to generate the vegetation drought monitoring results of Laibin City. Based on the climate, geography, and ecological characteristics of the monitored area in Laibin City, a specific analysis will be conducted to develop an appropriate TVDI index drought level, and generate vegetation drought level result maps for Laibin City in 2021, 2022, and 2023. Then, a detailed analysis of the vegetation drought situation in Laibin City is conducted according to time and space. Among them, in the past three years, the vegetation areas in Laibin City have experienced drought seasons mostly in summer and autumn. The interannual drought is mainly mild drought, and the proportion of areas with mild drought shows a relatively stable trend. In conclusion, TVDI proves to be a valuable tool for monitoring vegetation drought in Laibin City, offering insights for efficient water resource management strategies.

Periostin regulates the activity of keloid fibroblasts by activating the JAK/STAT signaling pathway

A keloid is secondary to skin trauma or has spontaneously manifested as an overgrowth and occurs when the skin heals abnormally after an injury. The main pathological manifestations are abnormal proliferation of keloid fibroblasts (KEL-FIB). This study researched periostin (POSTN) on keloid fibroblasts (KEL-FIB) and the associated mechanism, aiming to provide a reference for the targeted therapy of keloid. We got tissues from Second People’s Hospital of Guangxi Zhuang Autonomous Region between June 2022 and March 2023. POSTN expression was increased in keloid skin tissue and KEL-FIB than normal skin tissue and normal fibroblasts. We collected and inoculated KEL-FIB cells, transfection of si-NC (Silencing of POSTN negative control), si-POSTN (Silencing of POSTN), pcDNA-NC (Overexpression of POSTN negative control), and POSTN (Overexpression of POSTN) (Thermo Fisher Scientific) used Lipofectamine 2000 transfection reagent. Wound closure, cell proliferation viability, migrated cell numbers, and POSTN, p-JAK2, p-STAT3 protein levels were reduced in the si-POSTN group. Wound closure, cell proliferation viability, migrated cell numbers, and POSTN, p-JAK2, p-STAT3 protein levels were elevated in the POSTN group. POSTN protein levels did not changed and wound closure, cell proliferation viability, migrated cell numbers, were reduced in the POSTN+S-Ruxolitinib group. The study results indicated that POSTN promotes cell migration and proliferation by activating the JAK/STAT pathway, promoting KEL-FIB development.

A Delphi study to build consensus on strategies for adult vocational training of higher education institutes in Guangxi Zhuang Autonomous Region China

Adult vocational training of higher education institutes has great potential to help workforce coping with uncertainties of the fast-changing Labor market in the era of Industrial Revolution 4.0. However, the lack of clarity regarding strategies for effectively establishing adult vocational training in higher education institutes may hinder progress. The aim of this study was to develop agreed strategies of adult vocational training for higher education institutes in Guangxi Zhuang Autonomous Region, China. The 3-round Delphi method used semi-structured interviews and questionnaires to explore and reach an agreement on the future direction of the strategies. The semi-structured interview was used in the first round to explore the ideas in relation to such strategies. the questionnaires were used in the second and the third rounds to explore further and reach the consensus on expert opinions defined at interquartile range (IQR) ≤ 1. Besides that, Analytic Hierarchy Process (AHP) methods were used to calculate the weights of each consented statements. Through Delphi method, the comprehensive strategies have been articulated with the highlights of 1 vision, 6 goals and 40 actionable strategies. furthermore, the experts commonly highlighted that the vision of future adult vocational training shall prioritizes cultivating skilled professionals with industrial needs, offering everyone accessible opportunities for career development. Besides that, the result also showed that reskilling and upskilling and extending the leaners’ interpersonal networks have been ranked as the two most important goals for future adult vocational training in higher education institutes.

Smooth post-labial chaetae in Homidia (Collembola, Entomobryidae) and the description of four new species from China with the aid of DNA barcoding.

Four new species of Homidia are described from the Guangxi Zhuang Autonomous Region, China. Homidialongiantenna sp. nov. is characterised by its long antenna and slightly expanded post-labial chaetae; H.guangxiensis sp. nov. by the presence of smooth chaetae on the post-labium and posterior face of the ventral tube; H.huapingensis sp. nov. by the presence of smooth post-labial chaetae and pointed tenent hairs; and H.oligoseta sp. nov. by the pointed tenent hairs and fewer macrochaetae on Abdomen IV. Additions to the original description of Homidiaacutus Jing & Ma, 2022 are also provided.

Zhuang Food Culture: Meaning and Representation in the Process of New Media Communication in China

Designing a qualitative research method using observations, in-depth interviews, group discussions, and literature review data fieldwork survey collection investigated by participants to the meaning and representation of Zhuang food culture in the process of new media communication in China. The Zhuang people are a Tai-speaking ethnic group, mostly living in the Guangxi Zhuang Autonomous Region in Southern China. Food also plays a critical role in symbolizing happiness in Chinese culture. Zhuang food culture holds seven tiers of meaning for the Zhuang people, manifested in both traditional festivals and daily life. In Chinese culture, verbal communication is most accurately interpreted in the context of nonverbal communication, social hierarchy, and other background information. Social roles and a concern for respect and harmony inevitably have a bearing on communication. Zhuang food culture reveals that amidst the development of new media communication in China. Focuses on the Zhuang Sanyuesan Festival activities: A deeper understanding of the unique cultural characteristics of this ethnic group in new media communication; the Five-Color Sticky Rice; the Zhuang society and the outside world continuously are developing increasingly; the transformation of the Zhuang people through their production and life practices; etc., The representation of Zhuang food culture on new media platforms is primarily conducted through three dimensions: food culture, regional culture, and traditional Zhuang culture. Zhuang Food Culture has the remarkable ability to bring people together and create a sense of connection. For instance, dumplings, with their round shape and plump filling, symbolize wealth and prosperity and are often eaten during the Chinese New Year to usher in a prosperous year ahead.

A new species of Eotetranychus from China (Acari, Prostigmata, Tetranychidae), with an identification key to species of the genus Eotetranychus from China

ABSTRACT A new species of the genus Eotetranychus, Eotetranychus wuxuanensis sp. nov. collected on Rubus lambertianus (Rosaceae), is described from Wuxuan County, Guangxi Zhuang Autonomous Region, China, based on female and male adults of the species. In addition, the article reviews 42 species of the genus Eotetranychus from China, and provides an identification key with these species. http://www.zoobank.org/urn:lsid:zoobank.org:pub: 434BC39A-59FF-4A60-BF61-CB7E4758F68E

The fourth species of Sinamma Lin & Li, 2014 (Araneae, Tetrablemmidae) from China.

Sinamma Lin & Li, 2014 is a small tetrablemmid genus distributed in China, with three currently known species: Sinammaoxycera Lin & Li, 2014 from Guangxi Zhuang Autonomous Region and S.quadrata Tong & Li, 2022 and S.yingae Tong & Li, 2022 from Guangdong Province. While examining spider specimens obtained by sifting leaf litter from Danxiashan National Nature Reserve in Guangdong Province of China, we discovered a new species of the genus Sinamma, S.danxia sp. nov., which is described here.

Microbacterium rhizophilus sp. nov., an indole acetic acid-producing actinobacterium isolated from rhizosphere soil.

A novel gram-stain-positive, short rod, aerobic, non-motile and non-spore-forming actinobacterial strain, designated GXG1230T was isolated from the rhizosphere soil of a coastal mangrove forest in Beihai city, Guangxi Zhuang Autonomous Region, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXG1230T was affiliated with the genus Microbacterium. Additionally, it demonstrated a high degree of similarity to Microbacterium paludicola US15T (97.9%) and Microbacterium marinilacus YM11-607T (97.3%). Chemotaxonomic characteristics showed that the whole-cell sugars were glucose, xylose, rhamnose and galactose. Menaquinones MK-11 and MK-12 were detected as respiratory quinones. Lysine was found in the peptidoglycan hydrolysate and the polar lipids were diphosphatidylglycerol, one phospholipid and two unidentified glycolipid. The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The strain GXG1230T exhibited a genomic DNA G + C content of 71.7%. Furthermore, the average nucleotide identity values of GXG1230T with the reference strains were 75.4% and 81.9%, respectively, while the digital DNA-DNA hybridization values were 20.1% and 25.0%. Based on physiological, chemotaxonomic and phylogenetic information, strain GXG1230T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium rhizophilus sp.nov is proposed, with GXG1230T (= MCCC 1K09302T = KCTC 59252T) as the type strain.

Establishment and Application of a Triplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assay for Differentiation of PEDV, TGEV and PKV.

The pathogens responsible for porcine viral diarrhea are diverse, causing significant economic losses to the pig industry. PEDV and TGEV are well-known pathogens causing diarrheal diseases in pigs, leading to significant economic losses in the breeding industry. In contrast, the newly identified diarrhea virus, PKV, has not garnered as much attention. However, co-infection of PKV with PEDV results in more severe symptoms in piglets, such as acute gastroenteritis, and promotes increased replication of PEDV. Rapid and accurate diagnosis of viral diarrhea is essential for farms to identify pathogens early and mitigate economic losses. This study describes the development of a triplex real-time fluorescent quantitative RT-qPCR technique that can simultaneously detect three RNA viruses associated with porcine viral diarrhea: PEDV, TGEV, and PKV. To establish the triplex RT-qPCR method for the simultaneous detection and identification of the above three diarrhea viruses, conserved regions of the M gene of TGEV, the N gene of PEDV, and the 3D gene of PKV were selected to design specific primers and probes. After optimizing the reaction conditions, the method's specificity, sensitivity, and reproducibility were evaluated. The triplex RT-qPCR method did not show a significant difference in PCR efficiency compared to the single RT-qPCR method. The method is specific to TGEV, PKV, and PEDV, exhibits no cross-reactivity with other pathogens, and demonstrates satisfactory sensitivity and reproducibility; the limit of detection (LOD) of PEDV, TGEV, and PKV is 11.42 copies/μL. Furthermore, the performance of the triplex RT-qPCR assay was compared with the Chinese standard single-assay method for detecting TGEV, PKV, and PEDV, showing complete consistency between the two methods (100% compliant). Subsequently, 1502 clinical diarrhea samples were collected from the Guangxi Zhuang Autonomous Region to investigate the local prevalence of TGEV, PKV, and PEDV and the positive rates were 16.38% (246/1502), 1.46% (22/1502), and 45.14% (678/1502), respectively. Co-infection of PEDV and PKV were most common, with a rate of 12.12% (182/1502). This study presents a valuable method for the rapid and simultaneous identification of PEDV, TGEV, and PKV in clinical animal farming practices, and provides a reassessment of the epidemiology of these diarrhea-causing viral pathogens in the Guangxi Zhuang Autonomous Region.