Growth Related Indices Research Articles

Serratia marcescens LYGN1 Reforms the Rhizosphere Microbial Community and Promotes Cucumber and Pepper Growth in Plug Seedling Cultivation.

The vegetable plug seedling plays an important role in improving vegetable production. The process of plug seedling contributes to high-quality vegetable seedlings. The substrate composition and chemical fertilizer are widely studied to promote seedling growth. However, little is known about the effect of beneficial bacteria in the rhizosphere microbial community and vegetables' growth during plug seedling. The use of beneficial microbes to promote vegetable seedling growth is of great potential. In this study, we showed that the Serratia marcescens strain LYGN1 enhanced the growth of cucumber and pepper seedlings in plug seedling cultivation. The treatment with LYGN1 significantly increased the biomass and the growth-related index of cucumber and pepper, improving the seedling quality index. Specifically, LYGN1 also improved the cucumber and pepper root system architecture and increased the root diameter. We applied high-throughput sequencing to analyze the microbial community of the seedlings' rhizosphere, which showed LYGN1 to significantly change the composition and structure of the cucumber and pepper rhizosphere microbial communities. The correlation analysis showed that the Abditibacteriota and Bdellovibrionota had positive effects on seedling growth. The findings of this study provide evidence for the effects of Serratia marcescens LYGN1 on the cucumber and pepper rhizosphere microbial communities, which also promoted seedling quality in plug seedling cultivation.

Effects of Sodium Butyrate Supplementation in Milk on the Growth Performance and Intestinal Microbiota of Preweaning Holstein Calves.

The aim of the present study was to investigate the effects of sodium butyrate (SB) supplementation on the growth and intestinal microbiota of preweaning dairy calves. Eighty newborn Holstein calves (56 female and 24 male) were randomly allocated to four treatment groups with 20 calves each (14 female and 6 male). The suckling milk for the four treatments was supplemented with 0, 4.4, 8.8, or 17.6 g/d SB. During the 6-week experiment, dry matter intake was recorded daily, body weight was measured weekly, and rectal fecal samples were collected in the 2nd week. The V3-V4 hypervariable regions of the microbial 16S rRNA were amplified and then sequenced. SB supplementation elevated average daily gains (ADGs) in the first and second weeks. The optimal SB supplementation level for the whole preweaning period was 8.78 g/d, as revealed by analyzing the whole preweaning period ADG using second-order polynomial regression (quadratic) equations. The alpha diversity (Shannon diversity index), beta diversity, core phyla and genera, and function of the intestinal microbiota were affected by SB supplementation. In addition, the Shannon diversity index and core phyla and genera of the intestinal microbiota were correlated with calf growth-related indices. Overall, SB supplementation in suckling milk improved the growth performance and intestinal microbiota development of dairy calves in a quadratic manner, and regression analysis indicated an optimal supplementation level of 8.78 g/d.

SUSTAINABLE OKRA (ABELMOSCHUS ESCULENTUS L.) PRODUCTIVITY AS INFLUENCED BY FORTIFICATION OF GOAT MANURE WITH RICE HUSK ASH IN NORTHERN GUINEA SAVANNA ECOLOGY OF NIGERIA

To assess the agronomic effects of sustainable okra production as impacted by goat manure (GM) and rice husk ash (RHA) on a community-based demonstration farm in Gombe State, field trials were carried out during the wet seasons of 2017 and 2018. The trails consisted of four levels of GM and RHA, each measuring 0.0, 5.0, 7.5, and 10.0 t ha-1 which were used on Kwadon's local cultivar of okra in a Randomized complete block design (RCBD) replicated four times. Findings revealed that days to 50% emergence and establishment count were not significantly (P>0.05) influenced by the application of GM and RHA, regardless of rates of application. Other growth-related indices were significantly (P<0.05) higher due to GM, such as plant height at 4, 5, and 6 WAS, number of leaves plant-1 at 4 WAS, and pod diameter. On the other hand, RHA significantly (P<0.05) increased plant height, the number of leaves plant-1 at 5 and 6 WAS, leaf area, shortened days to 50% flowering, and the number of pods plant-1 across the sampling periods and seasons. Similar to this, due to adequate mineralization of organic material through time and space, fresh okra's marketable weight increased in the 2018 season compared to the previous year. Thus, the application of 10.0 t ha-1 of both GM and RHA can be adopted in the production of okra in the study area to reduce greenhouse gas emissions resulting from the use of inorganic agrochemical inputs.

Pregnancy supplementation of Gambian mothers with calcium carbonate alters mid-childhood IGF1 in a sex-specific manner.

Sex-specific effects of pregnancy calcium carbonate supplementation have been reported in 8-12 year old Gambian children, indicating faster growth in boys but slower growth in girls born to calcium-supplemented mothers. To determine whether the pregnancy supplement resulted in sex-specific effects on offspring IGF1 and other growth-related indices in mid-childhood. Analysis of archived data obtained in mid-childhood from the children of rural Gambian mothers who had been randomised to 1500 mgCa/d (Ca) or placebo (P) from 20 weeks pregnancy to delivery (ISRCTN96502494). Of the 526 children born and followed in infancy, 290 had early-morning, fasting plasma assayed for IGF1, IGFBP3, leptin, insulin and calcium-related indices and had anthropometry performed at age 7.5 (SD1.2) years (N/group: Males(M)-Ca = 64, Females(F)-Ca = 77; M-P = 76, F-P = 73). Sex-specific effects of maternal supplementation were considered using regression with sexes separated and together to test for sex ∗ supplement interactions. Boys had lower IGF1, IGFBP3, leptin and insulin than girls (P ≤ 0.004). IGF1 was higher in M-Ca than M-P (+14.2 (SE7.7)%, P = 0.05) but lower in F-Ca than F-P (-17.8 (SE7.4)%, P = 0.01); sex ∗ supplement interaction P = 0.001. IGF1 concentrations (ng/ml, geometric mean [-1SE,+1SE]) were M-Ca = 78.1[4.3,4.5], M-P = 67.8[3.4,3.6]; F-Ca = 99.5[4.8,5.1], F-P = 118.9[6.4,6.8]. Similar sex ∗ supplement interactions were seen for IGFBP3 and IGF1-adjusted-for-IGFBP3 but group differences were smaller. There were no significant supplement effects on the other biochemical indices. Calcium carbonate supplementation of pregnant Gambian mothers resulted in higher IGF1 in boys and lower IGF1 in girls during mid-childhood, consistent with the reported maternal supplement effects on growth of the offspring in later childhood.

Assessing Allelopathic Index for Estimating Allelopathic potential of Ajowan Extracts

Allelopathy is a procedure in which secondary metabolites are produced by plants. Some plants may beneficially or antagonistically affect other plants through allelochemical compounds which may be released directly or indirectly from live or dead parts and cause allelopathic and phytotoxic effects. The aim of the present study was to investigate the allelopathic possibility of ajowan (Carum copticum) extracts in different concentrations on morphological indices (germination and growth) of wheat (Triticum sativum), barley (Hurdeum vulgare), corn (Zea maize S.C 704), and safflower (Carthamus tinctorius L.) seedlings as proceeding crops and to develop a comprehensive formula for measuring the negative and positive effects of allelochemicals on growth indices such as shoot and root length, as well as the germination percentage on donor plants. It was demonstrated that increasing concentrations of ajowan extracts significantly decreased the Allelopathic Index, percent of germination, and other growth related indices of all seedlings. Based on the proposed Allelopathic Index of experimental plants, it was revealed that ajowan has posed a higher risk to corn and barley especially at the early stages of the growing cycle, while safflower and wheat indicated more tolerance to growth inhibition effects of ajowan in rotational sequence.

Broiler performance on finisher diets containing different levels of either Pleurotus ostreatus-fermented dried cocoa pod husk or dried cocoa pod husk supplemented with enzymes

The purpose was to investigate the feeding value of fibrous cocoa pod husk (CPH) either fermented with Pleurotus ostreatus or supplemented with fibre-degrading multi-enzymes (Viscozyme L + Pectinex 5XL) before inclusion in diets for 3-week-old finishing broilers. Growth performance and carcass yield of broilers fed diets containing 100, 200 or 300 g kg(-1) of the P. ostreatus-fermented CPH or 100, 200 or 300 g kg(-1) of the enzyme-supplemented CPH as well as a normal broiler finisher diet (control), were investigated in a 35-day feeding trial setup as a completely randomised design, with the seven treatments replicated three times. There were significant differences (P < 0.05) among treatments for some of the parameters measured including growth-related indices, percent weights of abdominal fat, intestine, and gizzard. It was concluded that P. ostreatus-fermented dried CPH up to a level of 200 g kg(-1) and enzyme-supplemented dried CPH up to a level of 100 g kg(-1) could be included in broiler finisher diets without any deleterious effects.

Developmental Polymorphism in a Newfoundland Population of the Hemlock Looper, &lt;I&gt;Lambdina fiscellaria&lt;/I&gt; (Lepidoptera: Geometridae)

The hemlock looper [Lambdina fiscellaria (Guenée)], a widespread and highly polyphagous Geometridae, is considered one of the most economically important defoliators of North American coniferous forests. Variations in the number of larval instars between geographic populations of this species have been previously reported in the literature. However, whether such developmental polymorphism occurs within a given population is unknown. In this study, we report the presence of both four and five larval instar individuals within a population of hemlock looper in Newfoundland when reared on balsam fir. For both sexes, the majority of individuals reared on balsam fir shoots went through four larval instars, but more than one third of the females (35.3%) went through five larval instars. Females with four larval instars developed faster and had smaller pupal weight than females with five larval instars. However, a growth-related index (weight gain per unit of time) was similar for the two ecotypes (four or five larval instars). No significant difference was observed between the two ecotypes in terms of reproductive capacity (fecundity and egg size). We also found significant differences in life history traits between males and females. Results indicate that developmental polymorphism, in this case, the variation in the number of larval instars, might provide some adaptive attributes that allowed exploitation of a broader ecological niche.

Developmental Polymorphism in a Newfoundland Population of the Hemlock Looper,Lambdina fiscellaria(Lepidoptera: Geometridae)

The hemlock looper [Lambdina fiscellaria (Guenée)], a widespread and highly polyphagous Geometridae, is considered one of the most economically important defoliators of North American coniferous forests. Variations in the number of larval instars between geographic populations of this species have been previously reported in the literature. However, whether such developmental polymorphism occurs within a given population is unknown. In this study, we report the presence of both four and five larval instar individuals within a population of hemlock looper in Newfoundland when reared on balsam fir. For both sexes, the majority of individuals reared on balsam fir shoots went through four larval instars, but more than one third of the females (35.3%) went through five larval instars. Females with four larval instars developed faster and had smaller pupal weight than females with five larval instars. However, a growth-related index (weight gain per unit of time) was similar for the two ecotypes (four or five larval instars). No significant difference was observed between the two ecotypes in terms of reproductive capacity (fecundity and egg size). We also found significant differences in life history traits between males and females. Results indicate that developmental polymorphism, in this case, the variation in the number of larval instars, might provide some adaptive attributes that allowed exploitation of a broader ecological niche.

Geographic biotype and host-associated local adaptation in a polyphagous species, Lambdina fiscellaria (Lepidoptera: Geometridae) feeding on balsam fir on Anticosti Island, Canada

AbstractThe debate about mechanisms underlying the evolution of host specialization by herbivorous insects remains open. Natural selection may act locally and lead to different patterns of geographic variation in life history traits of polyphagous herbivores. The hypothesis of genetically-based trade-offs in offspring performance on different hosts has been proposed but this has rarely been demonstrated. Under laboratory conditions, the biological performance of two populations of the hemlock looper Lambdina fiscellaria (Guenée), a highly polyphagous lepidopteran, was compared when reared on three different tree host species: balsam fir, eastern hemlock and sugar maple. One population originated from Anticosti Island, Québec, Canada, where the insect has evolved without having access to two of the three tree species tested, the other being from the mainland where all tree species are present. When reared on balsam fir foliage, which was naturally available to each population, larvae from Anticosti Island underwent four instars compared with five for the mainland population, indicating the existence of geographic biotypes in L. fiscellaria. When reared on the foliage of non-naturally available host trees, larvae from Anticosti Island had a higher incidence of supernumerary instars. This is a unique example where local adaptation to environmental conditions of an insect herbivore is expressed through a differential number of larval instars. Moreover, the Anticosti Island population showed a higher growth related index on the host available to both populations indicating that a fitness trade-off was the evolutionary process underlying the local adaptation of this population on balsam fir.

Geographic biotype and host-associated local adaptation in a polyphagous species, &lt;I&gt;Lambdina fiscellaria&lt;/I&gt; (Lepidoptera: Geometridae) feeding on balsam fir on Anticosti Island, Canada

The debate about mechanisms underlying the evolution of host specialization by herbivorous insects remains open. Natural selection may act locally and lead to different patterns of geographic variation in life history traits of polyphagous herbivores. The hypothesis of genetically-based trade-offs in offspring performance on different hosts has been proposed but this has rarely been demonstrated. Under laboratory conditions, the biological performance of two populations of the hemlock looper Lambdina fiscellaria (Guenée), a highly polyphagous lepidopteran, was compared when reared on three different tree host species: balsam fir, eastern hemlock and sugar maple. One population originated from Anticosti Island, Québec, Canada, where the insect has evolved without having access to two of the three tree species tested, the other being from the mainland where all tree species are present. When reared on balsam fir foliage, which was naturally available to each population, larvae from Anticosti Island underwent four instars compared with five for the mainland population, indicating the existence of geographic biotypes in L. fiscellaria. When reared on the foliage of non-naturally available host trees, larvae from Anticosti Island had a higher incidence of supernumerary instars. This is a unique example where local adaptation to environmental conditions of an insect herbivore is expressed through a differential number of larval instars. Moreover, the Anticosti Island population showed a higher growth related index on the host available to both populations indicating that a fitness trade-off was the evolutionary process underlying the local adaptation of this population on balsam fir.

Postflexion larvae and juvenile daily growth patterns of the Albor&amp;aacute;n Sea sardine (&lt;em&gt;Sardina pilchardus&lt;/em&gt; Walb.): influence of wind

The Alboran Sea is a hydrographically complex and variable area in which wind-driven coastal upwelling occurs. Its most abundant small pelagic resource is the sardine ( Sardina pilchardus ), which is subject to major interannual oscillations. Postflexion stages of sardine larvae were sampled in their main nursery grounds in the north Alboran Sea, and sardine juveniles were sampled periodically throughout the recruitment season. Daily growth analysis was used to identify periods favourable for larval survival and to assess the evolution of daily increment widths during the first months of life. The results showed that juveniles born later in the spawning season grew relatively faster than those born earlier. Two main growth phases were observed in juveniles: an initial one in which daily increment widths increased progressively, and a second one in which widths fluctuated, showing a decreasing trend. The beginning of the second phase was almost synchronous among different sub-cohorts, suggesting that it was triggered by environmental factors. The estimated mean daily growth rates during the larval phase were higher in surviving juveniles than in postflexion larvae born in the same month, supporting the “bigger is better” hypothesis in relation to larval survival. The influence of the environmental regime on growth was explored, and the evolution of a growth-related index, derived from otolith increment width variability, was compared with a single environmental descriptor, a “wind index” based on wind stress and direction. This analysis suggested that larval survival and larval and juvenile growth rates showed a positive correlation with NW component winds, associated with upwelling events on the continental shelf and calm sea weather conditions in the inshore nursery grounds. Conversely, Levantine and southern wind periods, which interrupt the upwelling and create rough seas in the inshore bays, lead to a decrease in growth rates in juveniles and low larval survival.

Combined method of image and cluster analysis to estimate the structural diversity of fungal communities

A method was designed to simultaneously estimate the diversity of fungal communities and growth-related indices. It is based on cluster analysis of image data of fungal colonies growing in agar plates. A total of 20 features (colony area, mean colours of the upper and lower colony sides as well as features describing the colour distribution) were recorded. The relative differentiating powers of these features were determined. Since the intensity of colours and their ratio to one another were dependent on the camera distance, colours were recorded at a constant distance of 36 cm. In contrast, growth rates were taken at a distance of 68 cm. Identification of a reproducible clustering level was favoured by the fact that, when similarity was reduced in dendrograms, the evenness in type distribution decreased at a higher rate than the number of image types and, in addition, exhibited distinct minima and intermittent maxima. In all data sets examined, abundant and rare image types, including singletons, always occurred at the first evenness minimum. Comparing fungal samples from a metal-contaminated arable soil and the relevant control showed the richness in image types and three growth-related indices (the average and the variation coefficient of growth rates as well as the portion of isolates with growth rates >3 mm day−1) to be greater in the control. Of these four indices, the former was the least sensitive and the latter the most sensitive indicator of metal stress.

Changes in Greenland Halibut Growth, Condition and Fecundity in the Northwest Atlantic (Flemish Pass, Flemish Cap and Southern Grand Bank)

The Greenland halibut fishable stock has been declining substantially since late-1980s, according to both surveys and commercial fishery indices, particularly among the ages 10+, which corresponds to the females age at 50% maturity. In this paper the effect of this apparent reduction in the stock abundance on growth and reproductive parameters is examined. Among growth parameters, the analysis of the first year growth is undertaken both by cohort and by geographic areas, assuming that density dependence might be the most severe at younger age classes. This is made using records of the respective first annual ring otolith diameters. Other growth related index such as the condition factor is also analysed. Neither significant differences in the first year growth have been observed between areas analysed, nor between cohorts, during the period 1988 to 1996. Mean condition factor-at-age for ages 1 to 13 were stable during this same period. About the reproductive parameters, two aspects are considered: the interannual variations in length-at-maturity and in the potential fecundity. Female length at maturity varied between 64.5 and 69.5 cm. Female potential annual fecundity ranged between 15 000 and 158 000. It increases with female age, but the mean fecundity-at-age variations were not significant either between years of sampling or between cohorts. A common feature observed is the relative stability of those characteristic through the period analysed, which could support a certain resiliency of the life history traits in this species.

Climatic conditions for crop production in Nordic countries

Climate is today a more determinant factor for yield level than it was in the past, when soil fertility was more important. The regional variation in radiation during the growing season in Nordic countries is less than that in temperature conditions. A combined plant growth-related index of these climatic factors accumulated during the growing season decreased from an annual value of 106 at 56°N (Ødum, East Jutland) to about 90, 60 and 44 at 60°N, 67°N and 70°N, respectively. The difference between coastal areas in Norway and the Finnish interior was either negligible or about 10% in disfavour of the coast at 67°N. When the moisture conditions of an imaginary pasture crop on a sandy soil were taken into account, the combined accumulated growth index of radiation, temperature and soil moisture was 71 at Ødum. On the Norwegian coast, the values were 72, 49 and 37, whilst in Finland, up to northeastern Norway, they were 56, 43 and 35 at 60°N, 67°N and 70°N, respectively. Plant production potential may be directly related to the accumulated growth index.

The effect of pyridoxine supplementation on dietary protein utilization in gilthead seabream fry

A study was undertaken to ascertain the effect of pyridoxine in diets of varying protein to energy ratio (P: E) for Mediterranean gilthead seabream fry (Sparus aurata) with respect to protein utilization. Diets were formulated to provide three levels of added pyridoxine (0·5, 5·0 and 50·0 mg/kg), at two protein levels (320 or 470 g/kg) whilst maintaining a similar gross isocalorific profile at 20 MJ/kg. Feeding level was proportionately 0·02 of the fish body weight per day, resulting in the following effects after a 70-day feeding trial. Low P: E ratio diets (15 g/MJ) did not perform as well as high P: E diets (24 g/MJ), with respect to growth related indices. Higher inclusions of pyridoxine into diets of equal P: E ratios, caused elevations in food conversion efficiency, protein efficiency ratio and apparent net protein utilization. A marked improvement of growth rate concomitant with pyridoxine level only occurred in the high P: E diets. It was concluded that incremental additions of pyridoxine in the diet, increased the efficiency of protein utilization for all diets tested. However, the protein sparing effect oflipid was not achieved for the low protein diets indicating the relatively high requirement for protein in the growth of the juvenile seabream.