Weight Growth Rate Research Articles

Role of body mass index in unbalanced (dysanaptic) lung growth of healthy infants.

Imbalance between forced expiratory volume in the first second (FEV1) and forced vital capacity(FVC) (dysanapsis) has been reported in children who are obese. This dysanaptic growth might begin at an early age, although there are no data on children younger than 6 years. To assess whether body mass index (BMI) and early weight gain, in healthy infants born at term, plays a significant role in the imbalance between FEV1 and FVC, even in the absence of obesity. Lung function was measured by means of raised volume rapid thoracic compression in 69 healthy infants born at term from the Nutrition in Early Life and Asthma cohort. Dysanapsis was defined as zFVC >0.674, zFEV0 .5 ≥-1.645, and FEV0 .5/FVC ≤-1.645. Weight gain (g/day) and growth rate (cm/year) were calculated as the difference between weight and length on the test date and those at birth. To assess the relationship between zBMI and dysanapsis, a receiver operating characteristic curve was performed. Multivariable analysis was carried out by means of linear regressions (one for each lung function index) and by logistic regression for dysanapsis (yes/no). Higher zBMI was associated with risk of dysanapsis (odds ratio: 3.53, [95% confidence interval: 1.30; 9.66]; p = .014): Each additional zBMI unit was associated with ~10 mL higher FVC and with ~3.5% lower FEV0.5/FVC. Weight gain was associated with lower FEV0.5/FVC ratio. Dysanaptic development of lung function begins very early in infancy and is related with weight gain and body mass index, even in the absence of obesity.

Profiling growth performance, insulin-like growth factors (IGFs), and IGF-binding proteins (IGFBPs) in rainbow trout lacking IGFBP-2b.

Insulin-like growth factor binding proteins (IGFBP) regulate insulin-like growth factor (IGF) signaling, but IGFBP-specific functions are not well characterized in fishes. A line of rainbow trout (Oncorhynchus mykiss) lacking a functional IGFBP-2b was produced using gene editing and subsequent breeding to an F2 generation. This loss-of-function model (2bKO) was subjected to either continuous feeding or feed deprivation (3 wk) followed by refeeding (1 wk). During continuous feeding, the 2bKO line displayed faster specific growth rate for both body weight and fork length, higher feed intake, and reduced feed conversion ratio compared to a wild type (WT) line. However, loss of IGFBP-2b did not affect the feed deprivation or refeeding response in terms of weight loss or weight gain, respectively. Several components of the IGF/IGFBP system were affected by loss of IGFBP2b. Total serum IGF-1 in the 2bKO line was reduced to 0.5 - 0.8-fold of the WT line although the concentration of free serum IGF-1 was not affected. Gene expression differences include reduced abundance of igfbp1a1, igfbp1b2, igfbp5b2, and igfbp6b1 transcripts, and elevated igf2 and igfbp6b2 transcripts in liver of the 2bKO line. Collectively, these findings suggest that although IGFBP-2b is a carrier of circulating IGF-1 in salmonids, the presence of IGFBP-2a and compensatory responses of other IGF/IGFBP system components support an anabolic response that improved growth performance in the loss-of-function model.

Effects of silybin on growth performance, antioxidant capacity and immunity in juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) fed with high-lipid diets

This research examined the impacts of varying silybin dosages in high-lipid diets on the growth, hepatic histology, immunity, and immune-related gene expression of juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂). The grouper (with an initial body weight of 8.27 ± 0.08 g) were fed diets containing silybin at levels of 0 g/kg (S1, control group), 0.05 g/kg (S2), 0.10 g/kg (S3), 0.15 g/kg (S4), 0.20 g/kg (S5), 0.25 g/kg (S6), and 0.50 g/kg (S7) for 8 weeks. The study results suggest that the silybin-treated groups displayed an initial increase followed by a decrease in final body weight and specific growth rate, with the highest value observed in the group S5. In serum samples, silybin supplementation of high-lipid diets resulted in increased activities of alkaline phosphatase (AKP), superoxide dismutase (SOD) and catalase (CAT) enzymes, decreased activities of aspartate transaminase (AST) and alanine transaminase (ALT) enzymes and increased total antioxidant capacity (T-AOC) content. In liver, the addition of silybin to high-lipid diets increased SOD, CAT, and lysozyme (LYZ) enzyme activities, increased T-AOC and immunoglobulin M (IgM) contents, and decreased reactive oxygen species (ROS) and malondialdehyde (MDA) contents. Contrasted with the control group, the addition of silybin at 0.05–0.50 g/kg enhanced the hepatic histomorphology, for example, ameliorating the indistinctness of cell outlines and diminishing the hepatocyte vacuolation. Silybin treatment significantly upregulated the expression of liver sod, cat, gpx, nrf2, keap1, hsp70, hsp90, tlr22, myd88, il-1β, tnf-α and il-6(P < 0.05). Silybin treatment significantly upregulated the expression of tlr22, myd88, il-1β, tnf-α, and il-6 of head kidney (P < 0.05). After vibrio harveyi challenge, groups S5-S6 showed higher survival than the control. Incorporating silybin into high-lipid diets boosts grouper's growth, antioxidant, and immune abilities. Regression analysis implies adding 0.23 g/kg silybin to the diets of juvenile hybrid grouper.

Effects of mandarin peel powder on growth, biochemical, immune, and intestinal health in Oreochromis niloticus at suboptimal temperatures

This 60-day study aimed to examine the efficacy of a diet supplemented with mandarin peel powder (MP) in enhancing the health and survival of Oreochromis niloticus under suboptimal temperature conditions (21 ℃). One hundred and eighty Nile tilapia fish (22.51 ± 0.04 g) were randomly distributed into four experimental groups; each of 3 replicates (15 fish per replicate). The first group (CONT) received a basal diet without MP. The second (MP10%), third (MP15%), and fourth (MP20%) groups were fed diets containing 10, 15, and 20% MP powder, respectively. At the end of the feeding trail, growth performance, serum growth hormone, α-amylase enzyme, lysozyme activity, nitric oxide, protease activity, globulin, serum levels of IL-1ß, antioxidant status, and intestinal histology were measured. The results showed insignificant differences between CONT, MP15%, and MP20% groups in the final body weight and specific growth rate. The growth hormones in the MP15% and MP20% groups did not show a significant difference compared to fish fed a normal basal diet (CONT). However, the amylase enzymes were significantly greater in both groups. The MP20% and MP15% groups showed a significant increase in antioxidant, lysozyme, nitric oxide, and protease activities compared to CONT. The results also showed that fish that were fed a diet with MP had significantly less of the pro-inflammatory cytokine interleukin-1 beta, and their intestinal villi got wider, especially in the MP20% group. It could be concluded that feeding tilapia on a diet with 20% MP is an effective strategy to improve their health when the temperature is below 21 °C. This is because the fish exhibit higher levels of antioxidant activity, reduced pro-inflammatory responses, and improved intestinal health without difference in the growth performance in compared to control group.

Optimizing feed restriction strategies for profitable Oreochromis niloticus culture in floating cages in riverine ecosystems

High feed cost is a major obstacle for promoting the growth of nutrient-rich fish species like Nile tilapia (Oreochromis niloticus) in cage aquaculture. To address this issue, cage farming of O. niloticus was studied for a 75-day period in the Gurukchi River of Bangladesh. Three different feeding strategies (FS) were tested in floating cages: FS5- weekly restriction on feed supply for two days, FS6- weekly restriction on feed supply for one day, and FS7- no weekly restriction on feed supply, with three replications for each treatment. Water quality parameters were monitored weekly and found within suitable levels for fish cultivation. The analysis of length–weight relationship and the condition factor of O. niloticus showed positive allometric growth regardless of the diverse feeding tactics employed that consistently exhibit stability across all treatments. Both FS6 and FS7 had significantly higher (p < 0.05) final individual length, final individual weight, net yield, total yield, and specific growth rate than FS5. FS6 yielded significantly higher fish production with significantly lower feed conversion ratio than FS7. Economic analysis revealed the highest benefit–cost ratio and net revenue in FS6, recommending its use to minimize the need for supplemental feed and reduce production costs in tilapia cage culture.

Sink Strength Dynamics Based on Potential Growth and Carbohydrate Accumulation in Strawberry Fruit

Fruit size and sugar content are key determinants of fruit quality, influenced by environmental factors and agronomic practices and sink strength provided by the genetic potential. Strawberry (Fragaria ×ananassa) produces fruits arranged in inflorescences, whose growth is affected by carbon competition between them. The competitive ability is termed as sink strength, which can be quantified as the potential growth rate under sufficient resource supply and/or no carbon competition among sinks, referred to as non-limiting conditions. Most previous studies did not observe potential growth, thereby failing to adequately evaluate sink strength and to assess the influence of environmental factors and agronomic practices on fruit growth. This study aimed to investigate the potential growth of strawberry fruits and analyze its sink strength dynamics. Non-limiting conditions were established by removing flowers to one fruit per inflorescence in a greenhouse experiment with plants grown in soil and given water and nutrients through drip irrigation. Fruits were harvested every 5 days from 5 to 55 days after anthesis (DAA), measuring the size, weight, and concentrations of major soluble carbohydrates in strawberry (sucrose, glucose, and fructose) and starch. Sink strength was represented by absolute growth rate based on fruit weight, and its components, sink size and sink activity, were represented by weight and relative growth rate, respectively. Fruit volume and weight showed a gradual linear increase at 5 DAA and then rapidly increased, following a single sigmoid curve between 30 and 55 DAA. Fruits primarily accumulated glucose and fructose during early growth, shifting to sucrose after 35 DAA. Starch concentration peaked at 5 DAA and then exponentially decreased. Sink strength exhibited a single peak between 40 DAA and 45 DAA. Sink strength gradually increased with sink size until 30 DAA, whereas sink activity significantly decreased until 30 DAA. Thereafter, sink strength and sink activity exhibited a peak, whereas sink size continued to increase. These results suggest that the major determinant of sink strength was sink size during early fruit growth, shifting to sink activity during late growth.

THE ABUNDANCE OF Chlorella Sp. AND Chlamydomonas Sp.: WATER QUALITY, GROWTH, AND FEED CONVERSION RATIO OF WHITELEG SHRIMP (Litopenaeus Vannamei)

Chlorophyceae groups, such as Chlorella sp. and Chlamydomonas sp., are a stable group of phytoplankton with the highest abundance in Litopenaneus vannamei ponds. These two types can be used as determinant factors for water quality, growth, and Feed Conversion Ratio (FCR) in L. vannamei farming. This study aims to determine the correlation of Chlorella sp. and Chlamydomonas sp. on the water quality, growth, and FCR in L. vannamei farming. This study was conducted by survey and identification of plankton, water quality, growth, and FCR. The identified plankton was conducted using Principal Component Analysis (PCA) on water quality, growth, and FCR. The results of the study obtained a total abundance of Chlorella sp. and Chlamydomonas sp. of 91.93%. The water quality was found that most parameters comply with standards for L. vannamei farming activities, except for the parameters phosphate, nitrate, nitrite, Total Organic Matter (TOM), and ammonia. The abundance of Chlorella sp. and Chlamydomonas sp. has a strong correlation with a parameter of water quality in all ponds. The growth rate of Mean Body Weight in ponds is 0.4 – 24.43 grams/shrimp and has a strong correlation with the abundance of Chlorella sp. and Chlamydomonas sp. according to the significance value (p-value) of approximately 0.004 – 0.636. FCR value is 0.3-2.23 and has a strong correlation with the abundance of Chlorella sp. and Chlamydomonas sp. according to the eigenvalue of 1.032-1.670. The eigenvalue of the pond represents approximately 54.364% (pond 1), 55.658% (pond 2), 1.236, and 1.032 (pond 3) of the variable diversity. The results of the study showed that the abundance of Chlorella sp. and Chlamydomonas sp. is an indicator that influences the environment quality, growth, and Feed Conversion Ratio.

Effect of Nitrogen and Humic Acid on Growth and Yield of Pakcoy (Brassica rapa subsp. chinensis) Plants

Decreased soil quality due to reduced nutrient content is one of the causes of decreasedquality and yield in plants. The use of humic acid organic matter with N fertilizer is one of the effortsto increase nutrients in the soil while increasing plant growth and yield. This research was conductedin Heuleut Village, Leuwimunding District, Majalengka Regency, from May to June 2024. Theresearch method used was the experimental method with a group-randomized design. The treatmentconsisted of 16 treatments that were repeated twice. The variables observed were plant height,number of leaves, root volume, plant fresh weight, leaf area, and plant growth rate. The resultsshowed that the use of N fertilizer at a dose of 115 kg/ha (urea 250 kg/ha) and humic acid 3 g/planthad a good effect on all variables observed. The results of soil analysis showed an increase in N-totaland C-organic.

Pertumbuhan Udang Galah (Macrobrachium Rosenbergii) yang Diberi Perlakuan Vegetable Floating Raft (VFR)

Galah shrimp is one type of freshwater shrimp that has great potential for cultivation. One of the obstacles to the growth of king prawns is water quality. This study was conducted to determine the effect of the installation of Vegetable Floating Raft (VFR) on the growth of king prawns (Macrobrachium rosenbergii). This study was conducted using the t-test method, with control and Vegetable Floating Raft treatments. The results showed that the growth pattern of weight and length of king prawns in each treatment was relatively the same. However, the daily weight growth rate of king prawns in the control treatment was greater at 3.36%, compared to the treatment of Vegetable floating raft at 1.26%, while the daily length growth rate of king prawns in the control treatment was 0.86%. The daily length growth rate in the Vegetable floating raft treatment was smaller at 0.61%. During the study the value of the degree of survival of the control treatment was 95%, while in the treatment of Vegetable floating raft the percentage of the degree of survival of king prawns was 62%. Based on the results of visual observations seen the rest of the feed and the rest of the skin during molting. It is suspected that shrimp are stressed due to changes in water quality characterized by increased ammonia levels in the last maintenance week caused by the death of kale plants in the maintenance medium.

Growth Performances and Productivities of Kappaphycus alvarezii and Eucheuma spinosum Cultivated with Long-line Method in PT. Sea Six Energy Indonesia

Indonesia is the second largest producer of seaweed in the world. Different cultivated seaweed commodities may have different production results. This study aimed to determine the effect of cultivation time and different types of seaweed on total weight and Specific Growth Rate (SGR) of Kappaphycus alvarezii and Eucheuma spinosum cultivated with the long-line method. The study was conducted in one cultivation area, PT. Sea Six Energy, Bali, Indonesia. Data collection was carried out through observations in 2 sites. Every site contained 3 square frame seaweed Kappaphycus alvarezii and Eucheuma spinosum. The sample used in this study was one thallus of seaweed taken randomly from 3 different ris ropes in each square frame. The average weight of thallus, total weight, and specific growth rate were performed in this study. After 51 days, the total weight of K. alvarezii was 1348.5 kg. The total weight of E. spinosum was 1398.7 kg. Both, K. alvarezii (8.4 ± 0.34 %.day-1) and E. spinosum (8.3 ± 0.02 %.day-1) showed higher Spesific Growth Rate (SGR) in the first 2 weeks of planting but not in the last 3 weeks of cultivation. There was no difference in productivity between K. alvarezii and E. spinosum cultivated using the long-line method. However, this research showed that the SGR value decreased as cultivation time increased.

High Salinity Intensive Seaweed Growth Rate in Aquaponic Systems

Aquaponics is a closed recirculating aquaculture method combined with hydroponics. This method is very alternative for raising fish while growing plants in specially designed media. The mechanism obtained is very effective and efficient, plants can utilize fish waste as nutrients for plant growth. Poisonous fish waste in ponds can be reduced by plants as nutrients for organic substances. This can enhance profitable bio-integrated food production systems. Plants will serve as vegetation filters. This study aimed to determine the effectiveness of aquaponic treatment in high-salinity water by observing the growth in three species of seaweed namely Gracilaria sp., Sargassum sp., and Kappaphycus alvarezii. The research design used the LSD method and was carried out for 63 days. Data analysis using Analysis of Variance (ANOVA) then continued with the test Duncan's Multiple Range Test. The research method involved making aquaponic media including preparing containers for seaweed cultivation and fish and shrimp farming, observing seaweed growth rates, and measuring water quality. Aquaponic treatment can increase the growth rate of intensively cultivated seaweed. The results showed the best weight growth rate in the aquaponic treatment of Gracilaria sp. with a value of 12.33 grams than Kappaphycus alvarezii 11.33 grams and Sargassum sp. 10.83 grams.

Comparative growth, survival, and feed conversion factor of the F1 generation of two new strains of tilapia Oreochromis niloticus produced by mass selection

Growth, survival, and use of feed of the F1 generation of two new strains of tilapia Oreochromis niloticus (GenBank Strain Y = OK078792 to OK078796 and Strain A = OK078797 to OK078807) produced by mass selection was compared with a known fast-growing commercial strain in Mexico (GenBank Strain Q = OR744820 to OR744838), during a grow-out phase of 165 days. Genetic variability including observed (Ho) and expected (He) heterozygosity, inbreeding coefficient (Fis), and paired comparisons of strain fixation index (Fst) of the three strains were also compared, and an additional analysis of molecular variance (AMOVA) was performed. Twelve hapas of 8.2 m3 (four for each strain) were stocked at a density of 25 org m−3 of 4.58 ± 0.67 g, which were fed with 45 to 25% crude protein feed under a dynamic feeding scheme of 8 to 3% live weight. Growth was estimated by growth rates in weight and length, and by the weight-specific growth rate (SGR). The use of feed was estimated by the total feed consumption rate and the feed conversion factor (FCF). Results indicate no significant differences (P > 0.05) in growth parameters of the three strains, except in SGR which was significantly higher in the control strain (Q) (2.75% day−1) as compared with strains Y and A (2.51 and 2.59% day−1 respectively). The average final weight and size of the fish were 314.6 ± 11.8 g and 25.5 ± 0.4 cm respectively. No significant differences (P > 0.05) were found in survival and feed utilisation among strains, with average values of 96.4% survival, feed consumption of 3.91 g day−1, and FCF of 2.08. Results of genetic diversity indicate that there were no significant differences (P > 0.05) in Ho and He of the three strains, with average values of 0.310 and 0.280 respectively. Fis values were negative and similar for the three strains, with a mean value of −0.109 which indicates no consanguinity in their populations. Finally, the paired comparisons of Fst were significantly different (P < 0.001), showing relatively low values but different from 0 (Y vs. A = 0.025, A vs. Q = 0.041 and Y vs. Q = 0.055), indicating slight but significant population differentiation between strains. In conclusion, the performance and genetic variability of the F1 generation of the two new strains suggest that valuable broodstock is now available for mass production of fry, with similar performance as to one of the best strains of tilapia in Mexico.

Lactobacillus salivarius and Berberine Alleviated Yak Calves' Diarrhea via Accommodating Oxidation Resistance, Inflammatory Factors, and Intestinal Microbiota.

Yaks are important food animals in China; however, bacterial diarrheal diseases frequently occur on the plateau, with limited effective therapies. The objective of this research was to evaluate the effectiveness of Lactobacillus salivarius (LS) and berberine in alleviating diarrhea in yak calves. For this purpose, eighteen healthy yak calves were divided into control (JC), infected (JM), and treatment (JT) groups. Yaks in the JT group were treated with 2 × 1010 CFU/calf L. salivarius and 20 mg/kg berberine, and yaks in the JM and JT groups were induced with multi-drug-resistant Escherichia coli. The results showed that the weight growth rate in the JM group was significantly lower than that in the JC and JT groups. The diarrhea score in the JM group was significantly higher than that in both the JC and JT groups. Additionally, the contents of T-AOC, SOD, GSH-Px, and IL-10 were significantly lower in the JM group than those in the JC and JT groups, while MDA, TNF-α, IL-1β, and IL-6 were significantly higher in the JM group. Microbiota sequencing identified two phyla and twenty-seven genera as significant among the yak groups. Notably, probiotic genera such as Faecalibaculum and Parvibacter were observed, alongside harmful genera, including Marvinbryantia and Lachnospiraceae UCG-001. Our findings indicate that treatment with L. salivarius and berberine significantly reduced diarrhea incidence, improved growth performance, and positively modulated intestinal microbiota, which could provide novel insights for developing new therapies for ruminant diarrhea.

An A2 β-casein infant formula with high sn-2 palmitate and casein phosphopeptides supports adequate growth, improved stool consistency, and bone strength in healthy, term Chinese infants: a randomized, double-blind, controlled clinical trial.

The aim of this randomized, double-blind, controlled trial was to examine the effects of infant formula on the growth, stool consistency, and bone strength of infants (n = 120) over a period of 4 months. The investigational group was fed an A2 β-casein cow's milk infant formula containing casein phosphopeptides (CPP) and high sn-2 palmitate (54% of total palmitate at sn-2). The control group was fed a standard cow's milk formula without CPP and with low sn-2 palmitate (29% of total palmitate at sn-2). The third group was fed human milk (HM) (n = 60). All three groups had similar baseline characteristics, and maintained similar BMI, sleep habits, and growth rates in body weight and length throughout the study. However, compared to the control group, infants in the investigational and human milk groups had significantly: (i) greater body length at 90, 120, and 150 days of age; (ii) greater growth rate in head circumference from 30 to 60 days of age, with larger head circumference at 60 days of age; (iii) larger daily stool frequency at 60, 90, and 120 days of age; (iv) softer stool at 60, 90, and 120 days of age; (v) higher bone quality index and bone speed of sound at 150 days of age; (vi) fewer hours of crying at 60 and 90 days of age; (vii) less abdominal distention, burp, and flatus at 60, 90, and 120 days of age; and (viii) less constipation at 90 days of age. At other time points, no significant differences were observed between the three groups. No serious adverse events (AEs) related to the study products were reported, and significantly fewer infants in the investigational and HM groups experienced at least one AE compared to the control group. The study suggests that the A2 β-casein formula with high sn-2 palmitate and CPP supports adequate growth, is well tolerated, and may have beneficial effects on stool consistency, gastrointestinal comfort, crying duration, and bone density, comparable to HM. Clinical trial registration: https://clinicaltrials.gov/, NCT04749290.

Growth characteristics and molecular identification of indigenous Limnospira strains from Ethiopian soda lakes as a protein source

Malnutrition poses a significant global challenge in regions such as Ethiopia, particularly in children with limited dietary diversity. Arthrospira, a protein-rich cyanobacterium known for its rapid growth even under extreme conditions, is a promising solution for biomass production. This study addresses knowledge gaps in the morphological characterization and taxonomic classification of Arthrospira by focusing on precise species identification and strategic strain selection for optimal biomass production. Cyanobacterial strains were cultivated in a modified Spirulina Ogawa Terui medium at 35 °C under the photosynthetically active radiation of 160 μmol photons m−2 s−1 with a 12/12 h light/dark period in laboratory conditions. One hundred Arthrospira-like strains were isolated and characterized based on their morphological attributes according to accepted criteria, from Ethiopian soda lakes. Systematic evaluation of the growth rate comparison test identified four exceptionally fast-growing strains, with LC-30 exhibiting the highest dry weight (3.27 ± 0.51 g L˗1) and specific growth rate (1.63 ± 0.05 d˗1) among them. DNA analysis confirmed that all four examined strains belong to the Limnospira genus, specifically identified as Limnospira fusiformis. LC-30 and LA-08 demonstrated protein and phycocyanin levels (582.80 ± 0.07 and 557.90 ± 0.09 mg g˗1, respectively) comparable to those reported for Arthrospira platensis, and surpassing conventional protein sources, thus suggesting their potential for nutritional supplementation. The study highlights the importance of bioprospecting indigenous strains from soda lakes, revealing promising candidates for large-scale biomass production.

Development and characterization of a novel intergeneric hybrid grouper (Cromileptes altivelis ♀ × Epinephelus lanceolatus ♂)

Distant hybridization often gives rise to offspring with hybrid vigor in plants and animals, and this method has also shown great potential in aquaculture. Grouper, an economically important marine species, has witnessed rapid growth in its related industry. To cultivate a new grouper variety with accelerated growth, superior meat quality, and high economic value, we successfully obtained fertilized eggs through artificial insemination, using humpback grouper (Cromileptes altivelis) as the maternal parent and giant grouper (Epinephelus lanceolatus) as the paternal parent. The hybrid groupers were successfully hatched and reared for up to 14 months. This study conducted comprehensive comparative analyses between the hybrid offspring and the parental species, focusing on embryo development, growth rate, morphological characteristics, chromosome karyotype and muscle nutrient composition. The hybrid grouper embryos completed their entire development, from fertilization to hatching, in an average duration of 20 h and 37 min, under controlled conditions of 28.5 ± 0.5 °C temperature and 28 part per thousand (ppt) salinity. Remarkably, the hybrid groupers exhibited significantly faster growth than humpback groupers, with an absolute weight growth rate 1.6 times higher. Additionally, the hybrid groupers displayed a 4.7% increase in meat yield compared to giant groupers. In terms of morphology, cluster analysis and principal component analysis were used to analyze the morphological framework data of the three grouper species, revealing that the hybrid groupers were more similar to giant groupers in their morphological framework. Chromosome karyotype and simple sequence repeats (SSR) analysis confirmed that the hybrid groupers have a total of 48 chromosomes, inheriting one set of chromosomes from each parent. This study provides a foundation for grouper hybrid breeding, and the hybrid groupers obtained in this research have the potential to become excellent commercial varieties.

Aluminum-induced oxidative stress promotes changes in the structure of the gut microbiota and liver deficiency

As a low-toxicity metal, aluminum has garnered increasing attention in relation to its impact on the human body; however, the specific mechanism of action remains unclear. To bridge this knowledge gap and facilitate practical applications, this study took 8-week-old ICR mice as the research object to study the effects of dietary addition of aluminum potassium sulfate on intestinal flora structure and liver. As the concentration of aluminum increased, it inhibited mice weight growth rate and significantly altered the composition of white blood cells in their bloodstream. Histological examination revealed liver inflammation through HE staining sections. The oxidative stress markers MDA increased, GSH-PX and CAT decreased significantly. And liver function index MAO increased, TC and ALP decreased first and then increased. Moreover, there was a significant increase in pro-inflammatory factor TNF-α content. Further 16S rRNA sequencing analysis demonstrated substantial changes in both composition and structure of mouse intestinal microbiota induced by aluminum exposure; microbial phenotype prediction indicated that aluminum-induced oxidative stress promoted an increase in abundance of oxidation-resistant microbial types. Alterations in gut flora structure also influenced the liver via the gut-liver axis. These findings lay a foundation for further research on the regulation and interaction of aluminum on intestinal flora.

Increasing levels of fishmeal replacement by defatted black soldier fly larvae meal reduced growth performance without affecting fillet quality in largemouth bass (Micropterus salmoides).

A 90-day feeding trial was conducted to evaluate the effects of replacing dietary fishmeal (FM) with defatted black soldier fly larvae meal (BSFL) on the growth performance and fillet quality of largemouth bass (Micropterus salmoides). The largemouth bass was divided into six groups (BSFL0, BSFL15, BSFL30, BSFL45, BSFL75, and BSFL100) and fed six isonitrogenous(CP 50%, 508 g/kg) and isolipid (CL 9%, 124 g/kg) diets, in which 0, 15%, 30%, 45%, 75%, and 100% of the fishmeal was replaced with BSFL, respectively. The results showed that the final body weight (FBW) and specific growth rate (SGR) of the largemouth bass decreased with increasing BSFL content, and they were significantly lower in BSFL75 than in BSFL0. The weight gain rate (WGR) decreased with increasing BSFL content and the feed conversion ratio (FCR) of largemouth bass increased with increasing BSFL content. The saturated fatty acids (SFAs) and n-3 polyunsaturated fatty acids (PUFA) contents of the largemouth bass fillet significantly decreased, and the n-6 PUFA content of the largemouth bass fillets significantly increased with increasing dietary BSFL. The fillet b* significantly decreased with increasing BSFL content. The biological parameters, fillet proximate nutrient composition, fillet amino acid composition, skin color, and fillet texture of the largemouth bass were not affected by the replacement of BSFL. Transcriptomic analysis revealed that BSFL replacement of FM affects the immune system and metabolic processes of largemouth bass through signaling pathways such as complement and coagulation cascades, the PPAR signaling pathway, cholesterol metabolism, and fat digestion and absorption. In conclusion, a replacement level lower than 45% BSFL was suggested for the overall growth and fillet quality of largemouth bass.

Динамика биологических показателей возвратных гибридов на основе кастера (калуга Huso dauricus × стерлядь Acipenser ruthenus) (Acipenseridae)

Due to the lack of conditions for industrial sturgeon fishing in the near future, the current artificial reproduction programs play a leading role in replenishing sturgeon populations and commercial sturgeon breeding. It may take a long time to bring the number of individuals and the total biomass of sturgeon fish species in different water basins into proper condition. It is important, along with the implementation of programs for the stocking of young sturgeons of various types of natural habitats, to develop commercial cultivation. Modern aquaculture is the use of environmental control systems, timely feed supply, rapid growth of juveniles with a minimum feed ratio, high survival rate at all stages of growth, greater yield of edible body parts during processing. The dynamics of size and mass indices and the Fulton fatness coefficient in recurrent hybrids of different ages (K × St) are considered × K, (K × St) × St, (St × K) × K and (St × K) × St. It is noted that the fastest growing hybrids turned out to be returnable hybrids based on K × St, and the hybrid (St × K) × St has the highest fatness coefficient with a slight growth rate of weight and length.

The Effect of Depth on Growth and Survival of Abalone Shells (Haliotis sp.) with A Multi-Level System

Abalone shells (Haliotis sp.), known as seven-eyed shellfish, are a type of gastropod. Abalone shells have high economic value accompanied by high market demand. The selling price of abalone reaches Rp. 250,000 (shells and meat). To optimize abalone cultivation activities with a long growth time, one method is needed for cultivating abalone shells in KJA (Floating Net Cages), namely using a tiered system in one hanger with different depths, namely 1m, 2m, 3m, 4m and 5m. so that it can increase land efficiency in cultivation activities. The aim of this research is to evaluate and analyze the effect of depth on the growth and survival rate of abalone shells (Haliotis sp.) using a multilevel system. The method used in this research was a Completely Randomized Design (CRD) experimental method with five treatments and three replications. The results of the research conducted showed that the results of the anova test on absolute length growth, specific length growth rate, absolute weight growth, specific weight growth rate, and survival of abalone shells (haliotis sp.) showed that they had no real influence (&gt;0.05) on the maintenance of abalone shells. Based on the research results, it can be concluded that using a tiered system at different depths does not have a real effect on the growth of abalone shells because the water quality conditions at a depth of 1-5 meters are optimal so they can support the growth of abalone shells.