Growth Performance Of White Shrimp Research Articles

Dietary Galla chinensis on white shrimp Penaeus vannamei: Promotes growth, nonspecific immunity, and disease resistance against Vibrio parahaemolyticus

This study investigated the impact of Galla chinensis (GC) powder as a dietary supplement on the immunity, growth, and pathogen resistance of white shrimp, Penaeus vannamei. An in vitro analysis demonstrated that GC inhibited Vibrio with no discernible impact on Lactobacillus. Following this, GC was tested for toxicity on white shrimp hemocytes. GC showed harmlessness to white shrimp hemocytes since cell viability was over 80% at all dosages. The immune response of hemocytes in white shrimp to diets containing varying concentrations of GC was evaluated in an in vivo experiment. Five feeding regimens were trialed for 28 days, which were designated as GC0.5, GC1, GC2.5, and GC5 (0, 0.5, 1, 2.5, and 5 g/kg, respectively). The enhanced total hemocytes, phenoloxidase, phagocytic activity, and production of superoxide anion in white shrimp on day 7 of the feeding trial by administering a supplementary GC diet significantly stimulated the nonspecific immunity of white shrimp. Additionally, dietary GC substantially increased the expression of related genes in white shrimp hemocytes in vivo, including Toll, Imd, antimicrobial peptides, antioxidant defenses, clotting protein, and heat shock protein. The challenge experiment was conducted to assess the disease resistance of white shrimp fed with GC. The shrimps were fed dietary GC for 7 days while being challenged with Vibrio parahaemolyticus. The shrimp exhibited a substantial variation in survival rate during the bacteria challenge experiment due to the activation of related genes and the enhancement of non-specific immunity. The mortality of white shrimp was substantially reduced by the supplementary GC at a concentration of 1 g/kg during the bacteria challenge. A final feeding trial was conducted to assess the effects of dietary GC on the growth performance of white shrimp. The continuous administration of dietary GC at a dose of 1 g/kg to shrimp for 56 days resulted in the highest enhancement in shrimp growth among all treatments. This was evidenced by significantly increased final body weight, weight gain, specific growth rate, and decreased feed conversion ratios. Dietary GC at a dosage of 1 g/kg has been suggested for use to enhance the growth performance of white shrimp and improve immunological responses, particularly nonspecific immunity and related gene expression, as well as resistance to Vibrio parahaemolyticus.

Effects of dietary supplementation with tropical microfungi Mucor circinelloides and Trichoderma harzianum powder on growth performance, bacterial communities, and the biomass nutritional profile of white shrimp grow-out culture (Litopenaeus vannamei)

Microfungi have been explored further as supplement constituents in shrimp culture due to their rich protein, lipid, essential amino acids, fatty acids, and various bioactive compounds. The researchers have evaluated the effects of two microfungi powders (Mucor circinelloides and Trichoderma harzianum) as feed supplements on growth performance, bacterial communities, and the biomass nutritional profile of white shrimp (Litopenaeus vannamei). The results showed that commercial/artificial feed (Feng-Li) with supplementation microfungi powder had a different effect on white shrimp growth performance than control after 62 days of the grow-out period. Supplementation with 1.5% M. circinelloides powder (M-2) could improve the growth performance of white shrimp, especially the survival rate (81.33%), final body weight (5.21 g), and shrimp biomass (211.35 g). In contrast, supplementation with 1.5% T. harzianum powder (T-2) did not improve the growth performance of white shrimp compared to control (p > 0.05). Furthermore, it also affected the profile of the bacterial community in culture water as well as in shrimp intestines. Supplementation with both microfungi could reduce the total number of bacteria in culture water at the end of the experiment, where the lowest number was obtained in treatment T-2 (3.9 × 10 5 CFU/mL), followed by M-2 (9.9 × 10 5 CFU/mL) and control (4.4 × 10 6 CFU/mL). In the culture water, 20 isolates of culturable bacteria were found during the trial, with the dominant bacteria in M-2: Bacillus cereus, Bacillus velezensis, Gordonia polyisoprenivorans, T-2: Acinetobacter sp., Gordonia polyisoprenivorans, Micrococcus luteus. Based on average well colour development (AWCD), the highest bacterial activity was in the M-2 (0.297–0.707), followed by T-2 (0.226–0.717) and control (0.094–0.694). The highest substrate group utilised in M-2 and T-2 were polymers (0.896 and 1.348) and control: amines (0.100). The highest Shannon diversity index was obtained in the M-2 (2.508), followed by control (2.286) and the T-2 (2.157). As many as 11 isolates of culturable bacteria were found in the shrimp intestine, with dominant bacteria in M-2: Bacillus velezensis, Micrococcus luteus, Bacillus cereus, T-2: Bacillus velezensis, Micrococcus sp.2, Demequina activiva. The highest bacterial activity was in M-2 (2.259), followed by control (2.020) and T-2 (0.050). The highest substrate group utilisation was in M-2: polymers (2.564), T-2: carbohydrates (0.094), and control: polymers (2.185). The highest Shannon diversity index was in the M-2 (3.234), followed by control (3.230) and the T-2 (0.751). In addition, 1.5% M. circinelloides and T. harzianum supplementation increased white shrimp muscle protein content up to 79.21% and 80.40%, respectively, compared to control (76.73%). In conclusion, using Mucor circinelloides powder as a feed supplement can improve the growth performance of white shrimp, increase the functional/probiotic bacteria: B. cereus and B. velezensis in cultur and shrimp's intestine, and increase the protein content in shrimp muscle tissue. However, more in-depth research is needed, especially on anti-nutritional compounds from fungi that affect shrimp growth.

Micro-Aid Liquid 10 Promotes Growth Performance and Health Status of White Shrimp, Litopenaeus vannamei

An experiment was conducted to evaluate the effects of the Micro-Aid Liquid 10 (MAL10) (DPI Global, Porterville, CA, USA), a product made from yucca extract, on growth performance, gut microbiota, and resistance of white shrimp, Litopenaeus vannamei against infectious disease caused by Vibrio alginolyticus. MAL10 was added to shrimp rearing water at different levels of 0 (control), 0.25 mL m3−1 (W0.25), 0.5 mL m3−1 (W0.5), 1 mL m3−1 (W1), and 5 mL m3−1 (W5), respectively, once per week for 70 days. Growth performances, including final body weight, specific growth rate, average daily growth and percentage of weight gain, were significantly improved by adding the MAL10 at levels up to 5 mL m3−1, which may be due to the proliferation of B cells in hepatopancreas of MAL10-treated shrimp. No significant differences in the total viable count and Vibrio-like count in the gut of shrimp were recorded by spread plate method. In the challenge test, shrimp reared in the water supplemented with MAL10 at levels of 1–5 mL m3−1 had significantly lower cumulative mortality after a challenge test with V. alginolyticus compared to shrimp reared in the control, W0.25 and W0.5 groups. Next-generation sequencing indicated that the relative distribution of phylum Proteobacteria in control (80.4%) was higher than the W (77.4%). The proportion of Vibrio was primarily dominant genera in the shrimp intestine and highest in the control group compared to the W group, followed by Spongiimonas, Motilimonas, Demequina, and Shewanella genera. Although there was no statistically significant difference, higher α-diversity indices were recorded in the W5-treated group than in the control group. Therefore, it is considered that MAL10 could be used as a natural alternative in shrimp aquaculture to reduce the risk of infectious disease caused by pathogenic Vibrio and improve the growth performance of white shrimp.

The utilization of black soldier fly larvae meal as a substitution of fish meal in diet for white shrimp, Litopenaeus vannamei, grow-out

Black soldier fly larvae (BSFL) meal is an environmentally friendly protein source due to its bioconversion production in organic waste processing and has it has potential as an alternative protein source for shrimp diet. Therefore, this study aimed to evaluate the utilization of BSFL meal as a substitution for fish meal in diet on the growth performance of white shrimp, Litopenaeus vannamei. The tested treatment were inclusion levels of BSFL meal namely 0%, 7.5%, 15%, and 22.5% to substitute fish meal in diet. The feeding experiment conducted using 12 of 50 L aquaria, and each equipped with gentle aeration. The white shrimp juveniles with initial weight of 1.20 ± 0.07 g were stocked at a density of 10 shrimp to every aquarium. The test diets were given as much as 8-3% BW/day (decreasing based on shrimp weight development) for 49 cultivation days. The results showed no significant difference between treatments (P>0.05) on the specific growth rate, weight gain, survival rate, feed efficiency, and protein efficiency ratio of white shrimp. In conclusion, the BSFL meal could be used up to the level of 22.5% to substitute fish meal in the diet for white shrimp grow-out.

The effects of dietary levels of the sea cucumber (Bohadschia ocellata Jaeger, 1833) meal on growth performance, blood biochemical parameters, digestive enzymes activity and body composition of Pacific white shrimp (Penaeus vannamei Boone, 1931) juveniles

The sea cucumber (Bohadschia ocellata) is a species with great density, reproduction rate and growth in the Persian Gulf. A Very few human consumption in Islamic countries makes this species a good choice to be used as a supplementary or stimulant ingredient in cultured aquatic animals. The present study evaluates the effects of dietary levels of B. ocellata meal on growth performance, blood biochemical parameters, digestive enzymes activities and body composition of Pacific white shrimp (Penaeus vannamei). White shrimp juveniles (6.6 ± 0.1 g) were fed with different levels of the B. ocellata meal (0, 2, 4, 6, and 8 % of the diet) during 8-weeks period. According to the results weight gain, specific growth rate, and daily feed intake trends showed significant (p <0.05) increase with increasing level of sea cucumber meal from 0% to 4%. The greatest feed conversion ratio and least protein efficiency ratio were recorded in the 8% treatment. In blood biochemical outputs, the least cholesterol and the highest glucose levels were recorded for the 4% and 6% treatments, respectively. Digestive enzymes activity assessments showed that protease activity was significantly increased from 4% to 8% treatments. In general, adding 4-6% of B. ocellata meal to commercial diet of P. vannamei juveniles clearly improved some body biochemical activities such as protease enzymes activity and blood cholesterol content, and enhanced growth performance of white shrimp.

Chitin derived from Daphnia similis and its derivate, chitosan, promote growth performance of Penaeus vannamei

Chitin isolated from Daphnia similis and its derivate, chitosan, were prepared to evaluate the effects on the improvement of growth performance of white shrimp, Penaeus vannamei. The diets containing 0.4% chitin or 0.4% chitosan were fed to shrimp for 56 days to evaluate the growth performance of shrimp. A diet without chitin or chitosan supplementation was used as the control diet (herein known as CD). No significant difference in survival of shrimp among groups was observed. The Shrimp fed a diet containing 0.4% chitosan significantly had much higher final weight, specific growth rate and percent weight gain as well as increased its protease activity in hepatopancreas. The feed conversion ratio was also increased in the 0.4% chitosan-fed shrimp. In addition, higher gene expressions of the chitinase (CHT) 3 and chitin synthase (CHS) in hepatopancreas, and the CHT6 and CHS in cuticle were also recorded in chitosan-treated shrimp as compared to CD-treated shrimp. Chitosan derived from chitin of D. similis is considered to have great potential to promote the shrimp growth, by increasing the digestive enzyme and the gene expressions of CHTs and CHSs.

Application of Probiotics for Organic Matter and Enhancement of Growth Performance in White Shrimp (Litopenaeus vannamei)

HighlightsLitopenaeus vannamei has been established as alternative to Penaeus monodon to shrimp farming in several countries.Increased importance of in situ microorganisms both in regulating biogeochemical cycles within the culture environment and in directly affecting shrimp growth and survival.To improve water quality in aquaculture is the application of probiotics and/or enzymes to ponds. AbstractIntensive shrimp farming has raised environmental issues due to aquaculture pollution, which is caused by uneaten feed, feces, organic matter, and toxic compounds (ammonia and H2S). One way to solve this issue is by using probiotics. Thus, this study aimed at investigating the effect of probiotic administration (Pseudomonas sp. and Bacillus sp.) at varying concentrations on total organic matter in the rearing water, feed conversion ratio, feed efficiency, and growth rate of white shrimp (Litopenaeus vannamei). This study used a Completely Randomized Design consisting of four treatments: T0 (without the probiotic supplementations), T1 (probiotics at 106 CFU/ml), T2 (probiotics at 107 CFU/mL), T3 (probiotics at 108 CFU/mL) with three replicates each. The results showed that the administration of probiotics at different concentrations had a significant effect on the total amount of organic matter, feed conversion ratio, feed efficiency, and specific growth rate of white shrimp. Administration of probiotics at a concentration of 107 CFU/ ml had the lowest organic matter in the rearing water, 138 mg /Lt. While probiotic administration at 108 CFU/ ml gave the lowest feed conversion ratio (1.22), the highest feed efficiency (81.57%) and the best growth rate (2.25%/day). This research suggests that the administration of 107-108 CFU/ ml probiotics could be applied to reduce organic content in the rearing water and improve the growth performances of white shrimp.

Growth performance of white shrimp Litopenaeus vannamei fed with Various dosages of prebiotic honey

The intensive culture system of white shrimp with high stocking density leads to slow shrimp growth performance. An alternative way to increase the growth performance of white shrimp is using a prebiotic. This study was aimed to evaluate the effectiveness of honey as a prebiotic through commercial feed to improve the growth performance of white shrimp. The white shrimp were reared with comercial feed that have been enriched with honey. This study consisted of four treatments, which were: control (without prebiotic), and the giving of honey at dosages of (A) 0.2%; (B) 0.4%; (C) 0.6%, each treatment with three replications. The average weight of vaname shrimp used was 0.44±0.11 g, maintained with a density of 270 shrimp/m-3 with an aquarium size of (60×30×30 cm3). The results of this study indicate that honey with a dosage of 0.6% results in the best growth performance with a growth rate of 0.14±0.002 g/day, specific growth rate of 4.70±0.02%/day, and feed conversion ratio of 1.53±0.02.

Effects of Vinegars and Sodium Acetate on the Growth Performance of Pacific White Shrimp, Penaeus vannamei

Vinegars and their salts have the potential to act as growth promoters and prophylactics against bacterial pathogens. This study aims to evaluate the effects of various vinegars and sodium acetate on the growth performance of white shrimp Penaeus vannamei. Groups of shrimps were fed diets containing 2% of either apple cider vinegar (ACV), coconut sap vinegar (CSV), sugar cane vinegar (CaV), or sodium acetate, and a diet with no vinegar (i.e. a control diet). Total acidity data of the diet showed that only the sodium acetate diet resulted in the highest total acidity after 60 min immersion in salt water (20 ppt). Attractability tests using customized repartitioned aquaria showed that the CSV diet attracted the highest significant percentage of shrimps after 10 min of feed placement in the feeding chamber. In the feeding trial that lasted for 60 days, results showed that the ACV and CSV groups of shrimps consumed significantly more feed than the other groups. All vinegar groups exhibited significantly higher final average body weight, weight gain, and specific growth, rate than either the control or sodium acetate group. The CSV group exhibited the significantly best feed conversion ratio and protein efficiency ratio. Survival was statistically similar among all groups. Conclusion: the CSV group exhibited the significantly best growth performance and efficiency while both the control and sodium acetate groups exhibited the poorest.

Dietary effect of apple cider vinegar and propionic acid on immune related transcriptional responses and growth performance in white shrimp, Litopenaeus vannamei

This experiment was conducted to study the effect of various levels of ACV® and Propionic acid (PA) on expression of immune related genes and growth performance in white shrimp (Litopenaeus vannamei). Three hundred and seventy-five shrimps with an average initial weight of 10.2 ± 0.04 g were collected and acclimatized for two weeks. Five experimental diets including control diet, 0.5% PA diet and 1%, 2% and 4% ACV® diets were applied to feed the shrimps. They were fed 4 times a day with 2.5% of body weight. After 60 days of culture, shrimps fed with ACV® and PA diets showed no significant difference in growth performance. Expression of prophenoloxidase (proPo), lysozyme (Lys), penaeidin-3a (Pen-3a) and Crustin (Cru) genes were determined from hepatopancreas, using the real-time PCR after 15, 30 and 60 days. Expression of Lys and proPo genes was significantly up regulated in shrimps fed with ACV® and PA diets compared to the control group after 30 and 60 days of treatment. After 15 days, Pen-3a gene expression was significantly higher in PA group compared to the control group. Also, shrimps fed with 1% and 4% ACV® and PA diets showed significantly increased expression of Pen-3a after 30 days. In contrast, expression of Cru was significantly down regulated in response to ACV® diets, but, Cru expression in treated shrimps with PA diet was greater than the control group after 30 and 60 days. Overall, the results provided evidence that ACV® could be used as a natural immunostimulant for shrimps in order to adjust and enhance expression of the immune related genes.

Replacement of Fish Meal with Soybean Meal, Alone or in Combination with Distiller's Dried Grains with Solubles in Practical Diets for Pacific White Shrimp, Litopenaeus vannamei, Grown in a Clear‐Water Culture System

AbstractThe objective of this study was to evaluate inclusion of distiller's dried grains with solubles (DDGS) as partial replacement of commercial, solvent‐extracted soybean meal (SBM) in fish meal‐free diets for Pacific white shrimp, Litopenaeus vannamei. Aquaria connected to a recirculating biofiltration system were utilized to evaluate growth, survival, and feed conversion of shrimp during the 8‐wk feeding trial. Each 110‐L aquarium was stocked with 15 shrimp (mean individual weight 0.99 g) and fed one of five diets: a diet containing 20% fish meal (FM), which served as the control (Diet 1); a diet containing 0% FM and 52.5% SBM (Diet 2); and diets containing 0% FM and either 10, 20, or 30% DDGS as partial replacement of SBM (Diets 3, 4, and 5, respectively). Shrimp were fed according to a pre‐determined feeding chart five times daily (0730, 1030, 1330, 1630, and 1930 h) and there were three replicates per dietary treatment. The results from the feeding trial demonstrated that final weight, weight gain (g), and percentage weight gain were significantly higher (P &lt; 0.05) for shrimp fed Diet 1 (10.96 g, 10.01 g, and 1051%, respectively) compared to shrimp fed diets containing DDGS; however, shrimp fed diets containing DDGS had similar (P &gt; 0.05) final weight, weight gain (g), and percentage weight gain as shrimp fed a diet containing 0% FM and 52.5% SBM (Diet 2). Feed conversion ratio (FCR) of shrimp fed Diet 1 (2.84) was significantly lower (P &lt; 0.05) compared to shrimp fed any other diet. Survival (%) was not different (P &gt; 0.05) among treatments and averaged 77.3% for the study. This study demonstrated that practical shrimp diets containing no FM had an adverse impact on growth performance of white shrimp when grown in a clear‐water system and that further research is needed to refine diet formulations when culturing shrimp in these systems when attempting to feed a diet without FM.

The effect of phytase levels in the diet on the digestibility and growth performance of white shrimp Litopenaeus vannamei

&lt;p&gt;This experiment was conducted to evaluate the effect of phytase levels in the diet on the digestibility and growth performance of white shrimp &lt;em&gt;Litopenaeus vanname. &lt;/em&gt;White shrimp were used in the trial with initial body weight of 3.8±0.01 g were reared in aquarium (60×40×50 cm&lt;sup&gt;3&lt;/sup&gt;) and filled with 60L treated sea water at a density of ten individu. Experimental diet were formulated in isonitrogeneous (36% protein) and isocaloric (3960 kcal/kg) to contain four levels of phytase: 0 iu/kg (diet A), 500 iu/kg (diet B), 750 iu/kg (diet C), and 1000 iu/kg(diet D). All diets were supplied with 0.5% of Cr&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3 &lt;/sub&gt;as an indicator for digestibility measurement shrimp fed three times daily at satiation levelfor how many days of rearing. A factorial completely randomized experimental design was selected consisted of four treatments and triplicates. The result showed that the phytase addition improved the digestibility of protein, calcium, and phosphor as well as improve growth performance of white shrimp. Based on evaluation of those parameters, it is concluded that phytase level of500 IU support the best growth performance ofjuvenile white shrimp.&lt;/p&gt; &lt;p&gt;Keywords: phytase, feed digestibility, growth,&lt;em&gt;Litopenaeus vannamei&lt;/em&gt;&lt;/p&gt;