Growth Of Thermophilic Bacteria Research Articles

Isolation and Molecular Profiling Of Thermoresistant Bacteria From Chimneys Of Baking Industries Of Madurai, Tamilnadu, India

The present study was chosen to discover the thermophiles isolation from chimneys of baking industries o Madurai, Tamilnadu, India. An attempt has been made to unearth the conserved sequences among them. Phylogenetic neighbour joining tree based on their 16S rRNA showing a relationship with related organisms has been constructed for each isolates keeping a species as an outgroup. In Madurai, baking industries have been found to be one of the hottest area having temperature as high as 120°C. Incubations of the bacterial colonies were done with covered water bath incubator at 70°C for 72-96 hrs in Castenholz TYE broth. The temperature range of 40-100°C, pH range of 4.0-10.0 and incubation period of 24-144 hrs was investigated for the best growth of thermophilic bacteria. Samples were collected in sterilized screw capped vials from different sites of Madurai Baking Industries and brought to the laboratory and kept at 4°C in the refrigerator till further processing. PCR products were sequenced using same primers by a commercial sequencing facility (Xcleris lab). The sequences of these ten bacterial isolates after sequencing were blasted using online NCBI BLAST program. Ten thermotolerant bacteria isolates were isolated and the optimal temperature for growth of the isolates was 70°C and the optimal pH was 7.0. 16S rRNA gene sequences of all these thermophilic bacterial isolates exhibited 98-99% homology with Bacillus sp. The phylogenetic analysis of the 16S rRNA gene sequences also confirmed the relationship of these thermophilic isolates with the genus Bacillus The 16S rRNA gene of all isolates was amplified, sequenced, and insilico analysis of these ten sequences was carried out. Based on BLAST alignment of 16S rRNA gene sequences of these isolates to GenBank sequences, these all isolates belonged to genus Bacillus and showed 98-99% similarity. The present study delves in to isolation, identification, characterisation,and analysis o the genomes of bacterial isolates such as Bacillus sp. VP1, Bacillus sp. VP2, Bacillus sp. VP3,Pseudomonas sp. VP4, Pseudomonas sp VP5,Bacillus licheniformis strain VP7,Bacillus sp. VP8, Bacillus sp. VP9, Bacillus altitudinis sp. VP10, and Bacillus cereus strain VP11.

Investigation on Growth of Oil Degrading Thermophilic Bacteria Isolated from Hot Spring

Oil spills are one of the key concerns of oil industry. The main distress of environmental specialists is the hazard to the marine ecosystem, caused due to offshore oil spills. In the present study the oil degrading potential of isolated bacteria on different media composition was carried out. From the study, it has been found that oil degrading thermophilic bacteria are capable of degrading soyabean oil, olive oil, tween 20, glycerol and crude petroleum oil. The culture of thermophilic was performed in thermus agar media and it was observed from the experimental study that the growth of thermophilic bacteria was moderately good at the range of 50oC to 60oC and 60oC to 70oC but declines after 70oC; no growth was observed in the range of 25oC to 50oC. It has also detected that the decaying capability of the thermophilic bacteria in Olive oil is initially better than soyabean oil during first six hours culture afterward its performance is better in soyabean oil than Olive oil. The present study is of special environmental significance as it can be efficiently used for bioremediation of oil polluted water.

Inhibitory Effects of Organic Acids on Bacteria Growth During Food Waste Composting

During the process of food waste composting, organic acids are generated from microbial breakdown of easily degradable substrates. These organic acids could inhibit microbial activities and sequentially reduce the decomposition efficiency. Considerable relationships among pH, organic-acid concentration and microbial activity were observed in this study. In order to systematically investigate inhibitory effects of four organic acids on composting bacteria, multivariate experimental designs were implemented in day 5 and day 9 during the initial stage of composting. Butyric and propionic acids had significant inhibitory effects on the growth of thermophilic bacteria on day 5. The inhibitory effects of organic acids on composting bacteria became significantly milder on day 9. The effects of different pH control amendments to mitigate microbial inhibition were also preliminarily examined. The treatment of NaAc resulted in the largest population of thermophilic bacteria.

Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana

BackgroundThe production of hydrogen from biomass by fermentation is one of the routes that can contribute to a future sustainable hydrogen economy. Lignocellulosic biomass is an attractive feedstock because of its abundance, low production costs and high polysaccharide content.ResultsBatch cultures of Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana produced hydrogen, carbon dioxide and acetic acid as the main products from soluble saccharides in Miscanthus hydrolysate. The presence of fermentation inhibitors, such as furfural and 5-hydroxylmethyl furfural, in this lignocellulosic hydrolysate was avoided by the mild alkaline-pretreatment conditions at a low temperature of 75°C. Both microorganisms simultaneously and completely utilized all pentoses, hexoses and oligomeric saccharides up to a total concentration of 17 g l-1 in pH-controlled batch cultures. T. neapolitana showed a preference for glucose over xylose, which are the main sugars in the hydrolysate. Hydrogen yields of 2.9 to 3.4 mol H2 per mol of hexose, corresponding to 74 to 85% of the theoretical yield, were obtained in these batch fermentations. The yields were higher with cultures of C. saccharolyticus compared to T. neapolitana. In contrast, the rate of substrate consumption and hydrogen production was higher with T. neapolitana. At substrate concentrations exceeding 30 g l-1, sugar consumption was incomplete, and lower hydrogen yields of 2.0 to 2.4 mol per mol of consumed hexose were obtained.ConclusionEfficient hydrogen production in combination with simultaneous and complete utilization of all saccharides has been obtained during the growth of thermophilic bacteria on hydrolysate of the lignocellulosic feedstock Miscanthus. The use of thermophilic bacteria will therefore significantly contribute to the energy efficiency of a bioprocess for hydrogen production from biomass.

Iron Demand by Thermophilic and Mesophilic Bacteria Isolated from an Antarctic Geothermal Soil

The thermophilic bacterial strain MP 4 assigned to a new species, likely of the genus Alicyclobacillus, was isolated from geothermal soils on the NW slope of Mount Melbourne, Antarctica. These soils have high iron concentrations and the strain MP 4 requires iron additions for growth. Four mesophilic bacterial strains Paenibacillus validus MP 5, MP 8, and MP 10, and P. apiarius MP 7, isolated from the same site, need iron supply for growth depending on the medium. Growth temperature of thermophilic strain ranges from 42 to 70 degrees C, and that one of mesophiles from 25 to 44 degrees C. Thermophilic and mesophilic strains shared microenvironments with temperature of 42-44 degrees C and showed optima of pH values ranging from 5.5 to 6.0. The thermophilic strain MP 4 reached values of 10(6) CFU ml(-1) in aqueous soil extract from the NW slope of Mt. Melbourne, and 10(5) CFU ml(-1) in water extracts from other geothermal Antarctic areas (Mt. Rittmann and Cryptogam Ridge). Growth of thermophilic bacteria in aqueous extracts of the NW slope of Mount Melbourne soils caused a reduction of 50% of soluble iron content, which was recovered in bacterial biomass. These results suggest a possible involvement of the thermophilic strain MP 4 in iron bioavailability in these geothermal soils.

The influence of yeast on thermophilic composting of food waste.

The microbial changes during thermophilic composting of food waste were investigated at 50 degrees C using a laboratory-scale composter. The results showed that an early increase in the growth of yeast was followed by the growth of thermophilic bacteria, and that subsequently, the yeast declined rapidly. The growth interactions of selected strains of yeast and bacteria were studied in culture media containing organic acids. The results demonstrated that the acidity of the media was reduced during the growth of the yeast. Therefore, it is concluded that in the early stages of composting, the growth thermophilic bacteria is inhibited by organic acids, and that the growth of yeast enables subsequent bacterial growth by removing organic acids from food waste.

Growth of thermophilic bacteria in rapemeal‐derived media

K.H. YEOMAN AND C. EDWARDS. 1992. A clarified, soluble, protein‐rich medium was developed from rapemeal by boiling with 0·1 mmol/1 NaOH. The medium contained 78% of the original meal protein and supported the growth of a range of thermophilic micro‐organisms that included Bacillus, Streptomyces and fungi. Amino acid analysis of the medium showed a full range of amino acids, and that acidic ones such as glutamate predominated. Growth of a thermophilic Bacillus spp. (termed 1/15) in the medium was biphasic and significant amounts of extracellular protease were synthesized during the scond growth phase. Biphasic growth was probably due to preferential utilization of free amino acids during the first phase of growth followed by degradation of proteins during the second phase.

The growth of thermophilic bacteria

The growth of thermophilic bacteria

The Problem of Transporting Milk in Hot Condition

Summary It is shown that the growth of thermophilic bacteria complicates the scheme for transporting milk in a hot condition. Another factor is the effect on the physical condition of the milk. Milk held at temperatures ranging from 50°C. (122°F.) to 60°C. (140°F.) for a period of twenty-four hours is usually coagulated. The type of fermentation at 50° C. (122°F.) appears to be different from that at 60°C. (140°F.). The results do not indicate that heat can be used as a substitute for cold during periods of transportation. This is particularly true with milk for direct consumption. It may be feasible to ship milk in a hot condition under conditions where milk is to be used for the manufacture of butter and where the transportation period is not over a few hours. Further study of this problem is however required.