Abstract Low survival (20–30%) and slow growth at early stages of Rohu, Labeo rohita have been major obstacles in meeting the demand of fry in Asia. Three experiments were conducted for 30 days each to evaluate the potential benefits of three multi-strain probiotics in three different stages of juvenile Rohu (hatchling—8 days old, fry—38 days old and advanced fry—68 days old) on the survival and growth. In this study, four different types of treatments were used; namely, T1: Control, i.e. feed without any dietary supplements, T2: feed with Lactobacilli, Bifidobacterium, yeast, Spirulina and phytase, T3: feed with Lactobacillus, yeast, seaweed and amylase, and T4: feed with Lactobacilli, Bacillus and yeast only. Hatchlings fed with T2 feed showed highest survival (74.7%), which is about 25% improvement in comparison to the survival of the Control group (49.9%). Similarly, T3 (60%) and T4 (61.5%) showed at least 10% increment in survival as compared to that of Control (T1). The hatchlings in T2 group had significantly higher (p 0.05) in final weight and specific growth rate among the treatments. Present study indicated that positive effects of multistrain probiotics on the survival and growth can be seen only at hatchling and fry stages, but not later stages.