Carapace Growth Research Articles

Condition Factors and Growth Patterns of Mantis Shrimp (Harpiosquilla raphidea) in the Estuary Waters of Berombang River, Labuhanbatu Regency, North Sumatera

Background: Mantis shrimp is one of the favorite shrimp caught by fishermen around the estuary waters of Berombang River, Labuhanbatu Regency. This study aimed to determine the condition factors and growth patterns of mantis shrimp population. Method: Three station points were determined using a purposive random sampling method. The sampling of Harpiosquilla raphidea was carried out using trawl nets. The data analysis provided information on growth patterns and condition factors. Result: The results showed that the growth patterns of male H. raphidea belonged to the positive b> 3 allometric category, where weight growth was faster than carapace growth. Moreover, the growth patterns of female H. raphidea belonged to the negative b<3 allometric category, where the carapace growth was faster than body weight growth. The value of Fulton (K) condition factors for male H. raphidea ranged from 3.54 to 13.18, and for females, it ranged from 15.28 to 22.09. The value of correlation analysis of Physic-chemical parameters, water temperature (0.935) and DO (0.832) showed the high category correlation test results. Conclusion: This result showed that from November 2021 to January 2022, the condition of H. raphidea population around the estuary waters of Berombang River was known and predicted to be in the adult stage

Ontogeny of a tessellated surface: Carapace growth of the longhorn cowfish Lactoria cornuta

Biological armors derive their mechanical integrity in part from their geometric architectures, often involving tessellations: individual structural elements tiled together to form surface shells. The carapace of boxfish, for example, is composed of mineralized polygonal plates, called scutes, arranged in a complex geometric pattern and nearly completely encasing the body. In contrast to artificial armors, the boxfish exoskeleton grows with the fish; the relationship between the tessellation and the gross structure of the armor is therefore critical to sustained protection throughout growth. To clarify whether or how the boxfish tessellation is maintained or altered with age, we quantify architectural aspects of the tessellated carapace of the longhorn cowfish Lactoria cornuta through ontogeny (across nearly an order of magnitude in standard length) and in a high‐throughput fashion, using high‐resolution microCT data and segmentation algorithms to characterize the hundreds of scutes that cover each individual. We show that carapace growth is canalized with little variability across individuals: rather than continually adding scutes to enlarge the carapace surface, the number of scutes is surprisingly constant, with scutes increasing in volume, thickness, and especially width with age. As cowfish and their scutes grow, scutes become comparatively thinner, with the scutes at the edges (weak points in a boxy architecture) being some of the thickest and most reinforced in younger animals and thinning most slowly across ontogeny. In contrast, smaller scutes with more variable curvature were found in the limited areas of more complex topology (e.g., around fin insertions, mouth, and anus). Measurements of Gaussian and mean curvature illustrate that cowfish are essentially tessellated boxes throughout life: predominantly zero curvature surfaces comprised of mostly flat scutes, and with scutes with sharp bends used sparingly to form box edges. Since growth of a curved, tiled surface with a fixed number of tiles would require tile restructuring to accommodate the surface's changing radius of curvature, our results therefore illustrate a previously unappreciated advantage of the odd boxfish morphology: by having predominantly flat surfaces, it is the box‐like body form that in fact permits a relatively straightforward growth system of this tessellated architecture (i.e., where material is added to scute edges). Our characterization of the ontogeny and maintenance of the carapace tessellation provides insights into the potentially conflicting mechanical, geometric, and developmental constraints of this species but also perspectives into natural strategies for constructing mutable tiled architectures.

Influences of dietary vitamin D3 on growth, antioxidant capacity, immunity and molting of Chinese mitten crab (Eriocheir sinensis) larvae

This study investigates the effects of vitamin D3 (VD3) on growth performance, antioxidant capacity, immunity and molting of larval Chinese mitten crab Eriocheir sinensis. A total of 6,000 larvae (7.52 ± 0.10 mg) were fed with six isonitrogenous and isolipidic experimental diets with different levels of dietary VD3 (0, 3000, 6000, 9000, 12000 and 36000 IU/kg) respectively for 23 days. The highest survival and molting frequency were found in crabs fed 6000 IU/kg VD3. Weight gain, specific growth rate, and carapace growth significantly increased in crabs fed 3000 and 6000 IU/kg VD3 compared to the control. Broken-line analysis of molting frequency, weight gain and specific growth rate against dietary VD3 levels indicates that the optimal VD3 requirement for larval crabs is 4825–5918 IU/kg. The highest whole-body VD3 content occurred in the 12000 IU/kg VD3 group, and the 25-dihydroxy VD3 content decreased with the increase of dietary VD3. The malonaldehyde content was lower than the control. Moreover, the superoxide dismutase activity, glutathione peroxidase and total antioxidant capacity of crab fed 6000 IU/kg VD3 were significantly higher than in control. Crabs fed 9000 IU/kg showed the highest survival after 120 h of salinity stress, and the relative mRNA expressions indicate vitamin D receptor (VDR) is the important regulatory element in molting and innate immunity. The molting-related gene expressions showed that the response of crab to salinity was self-protective. This study would contribute to a new understanding of the molecular basis underlying molting and innate immunity regulation by vitamin D3 in E. sinensis.

Population Density Effects on Carapace Growth in Clam Shrimp: Implications for Palaeontological Studies.

Fossil morphological data are time-averaged and generally reflect an overlap of different sources of carapace variability. To examine whether a proposed relationship between size and population density in fossil spinicaudatans is biologically meaningful, we set up rearing experiments involving two extant species: Eulimnadia texana and Eocyzicus argillaquus. Three and five days after hydration, clam shrimp were transferred into cups of various population densities that ranged between 1 and 15 inds/400 ml. Size and shape were measured 14 and 16 days after hydration, respectively. Every second day, we recorded length and sex of E. texana, which matured faster in lower-density cups. According to our growth model, population density and maximal carapace length follow a logarithmic relationship. At maturity, hermaphrodites yielded similar lengths across all population densities (~4.7 mm at 24°C), independent of age. Hence, clam shrimp can put off reproductive maturity as a response to decreased growth under higher density conditions. Growth rate generally decreases at maturity, but that effect is more pronounced in clam shrimp of high population densities, while low-density adults keep growing. For both species, multivariate analyses reveal that carapace size of low-density individuals is significantly larger than carapace size of higher-density individuals, while size values of intermediate densities cannot be distinguished. Shape distinction is strong in hermaphrodites of E. texana: 39.8% of the density-dependent shape variation is associated with relative umbo height, which is generally higher in individuals of smaller population densities. The H/L ratio, which is often used as a simple shape indicator, does not contribute to the main variation in shape, but it forms one of several ratios significant for 18.3% of the shape variability. In turn, the H/L ratio drives 30% of the shape variation in E. argillaquus. In addition, higher densities triggered shifts in ontogenetic growth trajectories in one third of the individuals, which led to aberrant morphologies. The present rearing experiment shows that some of the morphological variability on fossil bedding planes can be explained by population density. Also, it implies a considerable amount of ecophenotypic variability in Spinicaudata that affects our understanding of fossil taxonomy and palaeoecology.

Addition of Crude Fish Oil (CFO) on Feed Toward Growth and Feed Conversion Ratio of Mud Crab (Scylla serrata)

Mud crab is one of the fishery commodity which potential to be cultured because it has high economic value. Mud crab has nutrients content such as protein 62,72%, lipid 0,83%, ash 7,5% and water content 9,9%. Trash fish is fresh feed which commonly used as main feed for the growth of mud crab juvenile of 5,3to 13,8%, however Kuniran fish has omega-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) content which is necessary for optimum growth of mud crab.The study method was implemented as the experimental method use Completely Randomized Design (CRD) which consists of five treatments and four replications. The quantity of Crude Fish Oil which is added in trash fish feed on this study are: Treatment A (0%), B (2%), C (4%), D (6%) dan E (8%). The observed parameters are growth of mud crab and feed conversion ratio value. Data analysis use Variant Analysis (ANAVA) and continued with Duncan's Multiple Range Test to determine differences between treatments. The results shows that addition of Crude Fish Oil in trash feed fish for 32 days of rearing gives significantly different effect (P<0,05) toward length and width of mud crab carapace, meanwhile toward feed conversion value of mud crab doesn’t gives significantly different effect (P>0,05). High length and width growth of carapace were obtained on D and E treatment. The water quality of mud crab during 32 days of rearing is the temperature range of 28-29°C, pH range of 7,5-9,0, salinity range of 15-21ppt, dissolve oxygen range of 4 mg/l and ammonium range of 0,09-0,27 mg/l.

Growth performance and RNA/DNA ratio of noble crayfish (Astacus astacus) and narrow‐clawed crayfish (Pontastacus leptodactylus) fed fish waste diets

New and viable species for aquaponics and integrated multi‐trophic aquaculture (IMTA) in freshwater systems can improve yields and sustainability of aquaculture. Freshwater crayfish species such as Astacus astacus and Pontastacus leptodactylus are omnivorous feeders and considered candidates for feeding on faecal matters in existing aquaculture systems. Feeding trials were conducted to determine growth response and RNA/DNA ratio in freshwater crayfish fed fish waste. Carapace length and wet weight were measured to determine the growth response. Juvenile A. astacus was fed faeces of hybrid striped bass (Morone chrysops × Morone saxatilis) and rainbow trout (Oncorhynchus mykiss), while adult P. leptodactylus was fed with two commercial pellet diets and pikeperch (Sander lucioperca) faeces. The nutritional composition of hybrid striped bass faeces was close to optimal diet composition of A. astacus, and crayfish showed significantly higher carapace growth, weight gain and weight gain per moult as the group fed rainbow trout faeces. The growth of P. leptodactylus was significantly lower in terms of weight gain and weight gain percentage per moult for crayfish fed on pikeperch faeces. Thus, this study can recommend a co‐cultivation of hybrid striped bass and A. astacus within one system, but cannot recommend co‐cultivation of P. leptodactylus with pikeperch. Additionally, this study showed controversial results of RNA/DNA ratio and weight gain of both crayfish species. Thus, RNA/DNA ratio cannot be approved for investigations on crayfish physiological status in controlled feeding experiments if animals are fed with an inadequate diet.

Population Status of Green Turtles (Chelonia mydas) Foraging in Arica Bay, Chile1

Population characteristics and carapace growth rates were estimated for green turtles (Chelonia mydas) foraging in Arica Bay, northern Chile, from 2012 to 2016. Within the main foraging area of La Puntilla, an open population of up to 514 individuals was estimated by mark and recapture (Jolly-Seber method), with densities of 20–225 individuals/km2. Captured individuals (n = 159) ranged from 44.6 to 98.6 cm straight carapace length (SCL) with a mean SCL of 64.9 cm (SD 10.8); most turtles were juveniles; however, 10 were adults with SCL > 80 cm (three of these being males). A mean body condition index (BCI) of 1.64 (range 0.34–3.63) indicated healthy population conditions, with mean annual growth rates (AGR) of 4.6 cm/year (SD 2.97). The consistent presence of turtles between 2012 and 2016 and the relatively high abundance compared to the general situation reported for the species in Chile makes Arica Bay the most important feeding congregation of green turtles in the country. The population's healthy state and size, its location within an urban radius, and the multiple human uses of this site represent aspects that must be considered in protection and development plans for the city's coastal border.

Effect of Supplemental Heat in Captive African Leopard Tortoises (Stigmochelys pardalis) and Spurred Tortoises (CENTROCHELYS sulcata) on Growth Rate and Carapacial Scute Pyramiding

Carapacial scute pyramiding (CSP) is common in captive-raised turtles and tortoises. Several dietary and environmental hypotheses have been proposed to explain this phenomenon, but few have been scientifically investigated. The effect of increased heat exposure on CSP and growth was examined in juvenile African leopard (Stigmochelys pardalis) and spurred (Centrochelys sulcata) tortoises. Monthly measurements of individual tortoise surface temperature, length, height, width, weight, and pyramid height of treatment (heat) and control (no heat) groups for 2 years were higher in the treatment groups. Allometric comparison further revealed increased pyramiding in the treatment group. Humidity and diet did not differ between treatment and control groups. The results of this research investigation indicate that growth rate and CSP appear to be directly related and both increase with excess nocturnal heat. The significance of the atypical carapace growth and subsequent consequences for naturally occurring tortoise populations, especially in the context of global climate change, is unknown but requires further investigation.

An evaluation of the use of reptile dermal scutes as a non-invasive method to monitor mercury concentrations in the environment

Reptiles are ideal organisms for the non-invasive monitoring of mercury (Hg) contamination. We have investigated Hg bioaccumulation in tissue layers of reptile dermis as a basis for establishing a standardized collection method for Hg analysis. Tissue samples from freshwater turtle species Podocnemis unifilis and Podocnemis expansa and caiman species Melanosuchus niger and Caiman crocodilus, all from the Amazonian region, were analysed in this study. We first tested the relationships between Hg concentrations in keratin and bone to Hg concentrations in muscle to determine the best predictor of Hg concentration in muscle tissue. We then investigated the potential for measuring Hg concentrations across turtle carapace growth rings as an indicator of longer term changes in Hg concentration in the environment. Hg concentrations were significantly lower in bone (120ngg−1 caimans and 1ngg−1 turtles) than keratin (3600ngg−1 caimans and 2200ngg−1 turtles). Keratin was found to be a better predictor of exposure to Hg than muscle and bone tissues for both turtles and caimans and also to be a reliable non-invasive tissue for Hg analysis in turtles. Measurement of Hg in carapace growth rings has significant potential for estimating Hg bioaccumulation by turtles over time, but full quantification awaits development and use of a matrix-matched reference material for laser ablation ICPMS analysis of Hg concentrations in keratin. Realising this potential would make a valuable advance to the study of the history of contamination in mining and industrial sites, which have until now relied on the analysis of Hg concentrations in sediments.

Development of Enzymes andIn VitroDigestibility during Metamorphosis and Molting of Blue Swimming Crab (Portunus pelagicus)

The work focuses on development of digestive enzymes (amylase, total protease, trypsin, and chymotrypsin) and activity ratio of trypsin to chymotrypsin (T/C ratio) for digestive efficiency and growth, in blue swimming crab (Portunus pelagicus) during metamorphosis and molting. Specific activities of all enzyme parameters studied were associated with growth during metamorphosis, while only those of trypsin and T/C ratio were associated during molting cycle where trypsin and chymotrypsin specific activities associated with consumption rate with especially high levels during late intermolt and early premolt stages. About 50% increased weight gain was observed with at least double increased T/C ratio at the end of molting period, compared to the stages prior to molting. Growth of carapace would be more significant after finishing molting. Carapace width gain and T/C ratio were highest at the first crab stage. Studies ofin vitroprotein digestibility of different feed raw materials indicated thatArtemia, Rotifer, andMoinaare the best for larval stages. Otherwise, the use of shrimp feed andArtemiaflake could be the alternatives. Incorporating of cassava meal into the feed formula for early adult stage (juvenile) could be an advantage. The proteins from animals are more beneficial for adult crab culture than the proteins from plants and bacteria. The digestible quality of dietary protein is very important during larval stages, while the protein level of diet is more important during adult stages with fully developed digestive enzymes.

Population dynamics of the seabob shrimp Xiphopenaeus kroyeri (Dendrobranchiata, Penaeidae) in south-eastern Brazil

The population dynamics, including the sex ratio, reproductive period, individual growth and longevity, and population structure of the shrimp Xiphopenaeus kroyeri, are described. The shrimps were collected monthly from July 2005 to June 2007 at four sites in Ubatuba Bay, Brazil. The salinity, temperature, depth, organic matter content and sediment grain size were measured at each site. Specimens were quantified and sexed, their carapace length measured and their gonads were classified according to the stage of maturation. A von Bertalanffy model was used to estimate the individual growth, and longevity was calculated using its inverted formula. In total, 6 470 individuals were examined and the number of males and females differed significantly in favour of females. During some periods, the segregation could be related to the reproductive period. Reproductive females were captured throughout the sampled period, and there were biannual peaks that were followed by peaks of juveniles. Carapace growth was less for males than for females, which is likely related to oocyte production and the higher fecundity of the females. The higher probability of female capture can be related to their longer foraging to sustain the enhanced food requirement during the ovarian maturation period.

Post-larval morphology, growth, and development of Uca cumulanta Crane, 1943 (Crustacea, Decapoda, Ocypodidae) under laboratory conditions

Studies on the post-larval morphology and growth of crabs can allow correct identification of their early life stages, supporting studies on phylogenetic relationships and ecological aspects. Nevertheless, little is known about the juvenile morphology of ocypodoid crabs worldwide. Uca cumulanta Crane, 1943 is a fiddler crab commonly found in the intertidal zone of the tropical and subtropical western Atlantic. This study describes the morphology of the first juvenile stage of U. cumulanta, its absolute and relative growth, and the appearance and development of its secondary sexual characters. The antennules of U. cumulanta show morphological peculiarities and could probably be used as a distinguishing feature for crabs of this genus. The maxillule and the maxilla have sets of setae forming specialised structures for sorting particles from the sediment. The absolute growth pattern differed statistically between sexes. The study of relative growth revealed differences in the relationships cheliped propodus length (CPL), cheliped propodus height (CPH), and abdomen width (AW) vs. carapace length (CL). These differences between sexes showed that males diverge from females (in the chelipeds) from 3.04±0.11 mm (VI juvenile stage), and females diverge from males (in the abdomen) from 3.84±0.13 mm (VII juvenile stage). The pleopods, rudimentary in the first stage, disappear in the second stage, and then arise in different numbers for each sex from the third juvenile stage on. The spoon-tipped setae found on the second maxilliped, which are used to sort food particles, are evident only from the fourth juvenile stage and increase linearly with carapace growth. The absence of these setae may be the reason why juveniles settle on organic-rich substrates where they can obtain food.

A NEW SPINICAUDATAN GENUS (CRUSTACEA: ‘CONCHOSTRACA’) FROM THE LATE CRETACEOUS OF MADAGASCAR

Abstract: A new spinicaudatan genus and species, Ethmosestheria mahajangaensis gen. et sp. nov., is described from the Anembalemba Member (Upper Cretaceous, Maastrichtian) of the Maevarano Formation, Mahajanga Basin, Madagascar. This is the first spinicaudatan reported from the post‐Triassic Mesozoic of Madagascar. The new species is assigned to the family Antronestheriidae based on the cavernous or sievelike ornamentation on the carapace. Of well‐documented Mesozoic spinicaudatan genera, Ethmosestheria mahajangaensis is most closely related to Antronestheria Chen and Hudson from the Great Estuarine Group (Jurassic) of Scotland. However, relatively poor documentation of the ornamentation of most Gondwanan Mesozoic spinicaudatan species precludes detailed comparison among taxa. Ethmosestheria mahajangaensis exhibits ontogenetic trends in carapace growth: a change in carapace outline from subcircular/subelliptical to elliptical, and from very wide juvenile growth bands to narrow adult growth bands. Ornamentation style, however, does not vary with ontogeny. Ethmosestheria mahajangaensis individuals lived in temporary pools in a broad channel‐belt system within a semiarid environment; preserved desiccation structures on carapaces indicate seasonal drying out of pools within the river system. Specimens of Ethmosestheria mahajangaensis are preserved with exquisite detail in debris flow deposits; these are the first spinicaudatans reported from debris flow deposits. These deposits also contain a varied vertebrate fauna, including dinosaurs, crocodyliforms, turtles, and frogs. Rapid entombment of the spinicaudatan carapaces likely promoted early fossil diagenesis leading to highly detailed preservation.

Return to the wild: Translocation as a tool in conservation of the Desert Tortoise ( Gopherus agassizii)

Translocation could be used as a tool in conservation of the threatened Mojave Desert Tortoise ( Gopherus agassizii) by moving individuals from harm’s way and into areas where they could contribute to conservation of the species. Numerous factors may affect the success of translocations, including the conditions experienced by tortoises in holding facilities while awaiting translocation. The tortoises available for our translocation study had been provided supplemental water during their years spent in a captive holding facility, potentially inducing carelessness in water conservation. In addition to generally investigating the efficacy of translocation, we compared the effects of continuing with the effects of ceasing the holding facility’s water supplementation regimen. After exposure to one of the two water regimens, all tortoises were given the opportunity to hydrate immediately prior to release. We examined behavior, body mass, carapace length, movement, and mortality of tortoises for two activity seasons following release to the wild. Water supplementation was correlated with high rates of carapace growth and distant movements by males after release. Lengthy movements following translocation may be problematic for conservation planning, but this should be evaluated in light of the goals and circumstances of each translocation project. Although the mortality rate was 21.4% in 1997, data suggest that drought conditions at the site rather than the translocation itself negatively affected the tortoises. None of the tortoises died during their second season at the site. Our results indicate that translocation should be considered a useful tool in conservation of the Desert Tortoise.

Ciclo de mudas e taxas de crescimento de Metamysidopsis elongata atlantica (Crustacea, Mysidacea) cultivado em diferentes temperaturas e salinidades

Metamysidopsis elongata atlantica (Bascescu, 1968) foi cultivada em laboratório em diferentes combinações de temperatura (13, 20, and 25°C) e salinidade (25, 30, and 35‰) para estimar o período de intermuda e a taxa de crescimento da carapaça. A temperatura afetou o período de intermuda e a taxa de crescimento dos animais. Misidiáceos criados a 13°C tiveram maiores períodos de intermuda que aqueles cultivados a 20 and 25°C. Enquanto animais criados a 13°C tiveram maior longevidade, fêmeas não desenvolvem marsúpio quando cultivadas nesta temperatura. A taxa de crescimento estimada para misidáceos entre 0 a 14 dias (idade no início da maturidade) foi 0,02 mm/dia, 0,04 mm/dia, e 0,05 mm/dia para animais cultivados respectivamente a 13°C, 20°C e 25°C.

Immunolocalization and characterization of beta-keratins in growing epidermis of chelonians

Beta-keratins constitute most of the corneous material of carapace and plastron of turtles. The production of beta-keratin in the epidermis of a turtle and tortoise (criptodirians) and of a species of pleurodiran turtle was studied after injection of tritiated proline during the growth of carapace, plastron and claws. Growth mainly occurs near hinge regions along the margins of scutes and along most of the claws (growing regions). Proline incorporation occurs mainly in the growing centers, and is more specifically associated with beta-keratin synthesis. Proline-labeled bands of protein at 12–14 kDa and 25–27 kDa, and 37 kDa, in the molecular weight range of beta-keratins, were isolated from the soft epidermis of turtles 3 h after injection of the labeled amino acid. After extraction of epidermal proteins, an antibody directed against a chicken beta-keratin was used for immunoblotting. Bands of beta-keratin at 15–17 kDa, 22–24 kDa, and 36–38 kDa appear in all species. Beta-keratin is present in the growing and compact stratum corneum of the hard (shell) and soft (limbs, neck and tail) epidermis. This was confirmed using a specific antibody against a turtle beta-keratin band of 15–16 kDa. The latter antibody recognized epidermal protein bands in the range of 15–16 kDa and 29–33 kDa, and labels beta-keratin filaments. This result indicates that different forms of beta-keratins are produced from low molecular weight precursors or that larger aggregate form during protein preparation. The present study shows that beta-keratin is abundant in the scaled epidermis of tortoise but also in the soft epidermis of pleurodiran and cryptodiran turtles, indicating that this form of hard keratin is constitutively expressed in the epidermis of chelonians.

Ciclo de vida pós-marsupial e crescimento de Metamysidopsis elongata atlantica (Crustacea, Mysidacea, Mysidae) em cultivo de laboratório

Metamysidopsis elongata atlantica (Bacescu, 1968) was reared in the laboratory for 45 days at 20±1°C and salinity of 30ppt. Growth curves (von Bertalanffy model) were calculated for both sexes and for each sex. The daily rate of carapace growth was significantly different between females and males (F test, p <0.05). Before the sexual maturity (14 days), the growth rate of females was higher than that of males (females, 0.0457 mm day-1; males, 0.0448 mm day-1). After the maturity (15 to 45 days), these rates decreased similarly for both sexes (females, 0.0203 mm day-1; males, 0.0174mm day-1). The average growth rate was 0.0207mm day-1 over the 45 days. Twelve molts were observed in a period of 60 days. The first five molts occurred up to 14 days old (age of the sexual differentiation), with a mean intermolt period of 2.9 days. From the 6th molt it increased to 5.6 days. The results suggest that the use of the carapace length is a good measure to calculate the growth and longevity of the organisms.