Grouting is one of the most commonly used methods to control water inrush disasters in fractured rock mass. At present, most of the research on the diffusion process of fracture grouting is carried out on the hypothesize that the groundwater was completely displaced by the slurry, which is lack of sufficient theoretical basis. Therefore, a one-dimensional visual fracture grouting experimental system was developed and a new characterization method of slurry concentration based on the image gray level was proposed in this paper in order to characterize the variation law of slurry-water mixing region during the grouting process. Results show that there exists slurry-water mixing region at the frontal surface of the diffusion slurry in the grouting process in fractured rock mass. The slurry-water mixing region can be characterized by the gray level of the images captured by cameras. The relationship of water to cement ratio of slurry (w/c) and image gray level of slurry (G) conforms to linear function, which can be expressed by G = 19.17 w/c when w/c is between 0.8 and 10. In addition, slurry concentration decreased linearly along the diffusion flow path in the slurry-water mixing region. The size of slurry-water mixing region is positively related to grouting flow rate, the mass ratio of water to cement (w/c) and fracture aperture when the grouting flow rate is between 0.6 L/min to 2.4 L/min, w/c is between 0.8 and 1.6 and fracture aperture is between 1 mm to 5 mm. Furthermore, the ratio of slurry-water mixing region size to hypothetical diffusion radius (RSR) was proposed to characterize development degree of slurry-water mixing region. The stable value of RSR was between 0.04 and 0.24 under the experimental conditions in this paper. The index of RSR can be used to provide theoretical basis for the simplification of the interface between slurry and water. The distribution rules of the slurry-water mixing region can be applied to predict the diffusion range more accurately and perfect the grouting theory.