A series of subsea tunnel projects with unprecedented scale is under vigorous construction. In the construction sector, grouting has been widely adopted as the primary method for disaster control, and superplasticizers are the additives commonly used in grouting engineering. However, during grouting, seawater intrusion and ambient temperature change lead to significant changes in the rheological properties of grouting materials and failure of the grouting scheme. Therefore, it is critical to study the rheological properties of seawater-mixed grout slurry containing a polycarboxylate superplasticizer (PCS) at different temperatures. In this study, the mechanism of a PCS and its effects on the rheological properties of biomass-activated grouts (BAGs) blended with seawater were revealed for the first time. Further, the rheological characteristics and constitutive equations of seawater-mixed grout slurry at 0–30 °C were determined. Results indicated that the PCS significantly reduced the shear stress and viscosity of seawater-mixed grout. The fluidity of grout slurry with 0.8 % PCS increased by 30.8 %. The power law model was applied to describe the rheological characteristics of seawater-mixed slurry with varying PCS contents. With increasing temperature, the rheological index increased, whereas the viscosity, shear stress, and consistency coefficient decreased. The constitutive equation of seawater-mixed BAGs containing 0.8 % PCS at different temperatures was also established for the first time. The findings of this study can provide a basis for grouting diffusion theory, numerical calculation, and the design of a grouting scheme for subsea tunnels at different temperatures.