Serum Levels In Group Research Articles

Genotoxicity of Streptozotocin versus High Fat Diet-Induced Hyperglycemia in Adult Albino Rat Myocardium

Background: Hyperglycemia has detrimental systemic and cardiac effects by increasing oxidative stress and advanced glycation end products (AGE). Prolonged hyperglycemia-induced DNA damage in various tissues. Aim: To compare the hyperglycemia-induced genotoxicity of STZ versus a high-fat diet (HFD) on rat myocardial tissue. Methods: Sixty-six adult male rats were allocated randomly into 3 groups of 22 rats each; Group I was maintained on a standard chow diet, Group II where rats were injected with STZ (40 mg/kg, intraperitoneally) for 5 consecutive days, and Group III where rats were fed HFD for 15 weeks. After reaching the hyperglycemic level (> 150 mg/dl), all rats were kept for 5 weeks on HFD to ensure the occurrence of DNA damage. Rats were euthanized and sacrificed at the end of the experiment. Blood samples were collected from the tail vein. The heart was excised carefully and processed for histological (Hematoxylin & eosin) and immunohistochemical staining (?H2AX). Results: The HFD group's serum levels were significantly higher for NO and MDA than the STZ group's serum levels, whereas GSH levels were lower. On histopathological examination, congested blood vessels, inflammatory cells, and spacing between cardiac muscle fibers were seen in Groups II and III. Regarding ?H2AX immunostaining, the reaction was marked in Group III as compared with Group II. Conclusion: Hyperglycemia induced deteriorative changes in the myocardial tissue. HFD-induced hyperglycemia produced much genotoxic damage to the myocardium. This could be related to its dramatic oxidative damage.

Therapeutic effects and safety of apatinib mesylate on patients with gastric carcinoma peritoneal metastasis in SOX scheme.

The aim of this study was to investigate the diagnostic values of serum tumor markers in gastric carcinoma peritoneal metastasis and the therapeutic efficacy as well as safety of apatinib mesylate combined with Geo+Oxaliplatin (SOX) scheme treatment in gastric carcinoma peritoneal metastasis. Sixty patients with gastric carcinoma peritoneal metastasis and 11 patients without gastric carcinoma peritoneal metastasis were selected as the research subjects. The levels of serum tumor markers [carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 125, CA211, CA242, CA724, and CA19-9] and abdominal irrigating solution exosome [micro ribonucleic acid (miR)-21 and miR-320c] and the differences in their diagnostic values were compared and analyzed. The patients with gastric cancer peritoneal metastases are then divided into two groups, one for control (30 cases receiving just SOX scheme treatment) and the other for the experiment (30 cases receiving SOX scheme treatment plus apatinib mesylate). Besides, the differences in serum tumor marker level, therapeutic efficacy, overall survival (OS), complication rating, and Quality of Life Questionnaire-Core-30 (QLQ-C30) score among patients after treatment were compared. Demonstrated that serum carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 125, CA211, CA242, CA724, and CA19-9 levels of patients in the transfer group were remarkably enhanced compared with those of patients in the non-transfer group, and the levels of abdominal irrigating solution exosome (miR-21 and miR-320c) were reduced compared with those in non-transfer group (p<0.05). The area under the curve (AUC) of the diagnosis of gastric carcinoma peritoneal metastasis by each index were 0.553, 0.880, 0.832, 0.619, 0.863, 0.651, 0.918, and 0.903, respectively. Patients in the experimental group's serum levels of CEA, CA125, CA211, CA242, CA724, and CA19-9 were noticeably lower after therapy compared to those in the control group, and their median OS was also noticeably longer (p<0.05). After treatment, the objective remission rate (ORR) and disease control rate (DCR) of the control group and experimental group amounted to 6.7% vs. 30.0% and 50.0% vs. 86.7%, respectively. ORR and DCR of the experimental group were notably higher (p<0.05). Between the patients in the control group and the experimental group, there were no glaring variations in the frequency of problems (hypertension, nausea, vomiting, bone marrow suppression, hand-foot syndrome, and leucopenia) (p>0.05). The cognitive function, emotional function, and life health scores of patients in the experimental group were significantly higher than those in the control group (p<0.05), which suggested that serum tumor markers and miR-21 as well as miR-320c showed high diagnostic efficiency in gastric carcinoma peritoneal metastasis. Apatinib mesylate combined with SOX scheme treatment was more effective in treating gastric carcinoma peritoneal metastasis and possessed the same safety as single SOX scheme treatment. Hence, it is worthy of clinical promotion.

Diagnostic sensitivity and specificity of combined detection of serum levels of OPN, MG7-Ag, TPS for gastric cancer

Objective To explore the combined detection of serum osteopontin(OPN), gastric cancer associated antigen(MG7-Ag) and tissue polypeptide specific antigen(TPS) for the clinical value of diagnosis of gastric cancer. Methods Enzyme linked immunosorbent test(ELISA) was used to detect serum levels of OPN, MG7-Ag and TPS of 60 cases of gastric cancer patients, gastric benign lesion group 20 cases were randomly selected at the same time, the group of 24 cases of precancerous lesions and 40 healthy people were also selected as control study. Results Gastric cancer group's serum levels of OPN, MG7-Ag and TPS were significantly higher than those of gastric benign lesions, the precancerous lesion group and healthy control group(F=50.993, 62.50, 41.396, all P<0.01). The single detection sensitivity were 71.7%, 80.0% and 67.3% respectively, three joint detection sensitivity was 91.7%. Conclusion Combined detection of serum OPN, MG7-Ag and TPS can improve the sensitivity of diagnosis of gastric cancer, and has higher diagnosis significance in the diagnosis of gastric cancer. Key words: Stomach neoplasms; Osteopontin; Antigens; Neoplasm

Effect of leucine supplementation on indices of muscle damage following drop jumps and resistance exercise

The purpose of this study was to determine the effect of leucine supplementation on indices of muscle damage following eccentric-based resistance exercise. In vitro, the amino acid leucine has been shown to reduce proteolysis and stimulate protein synthesis. Twenty-seven untrained males (height 178.6±5.5 cm; body mass 77.7±13.5 kg; age 21.3±1.6 years) were randomly divided into three groups; leucine (L) (n=10), placebo (P) (n=9) and control (C) (n=8). The two experimental groups (L and P) performed 100 depth jumps from 60 cm and six sets of ten repetitions of eccentric-only leg presses. Either leucine (250 mg/kg bm) or placebo was ingested 30 min before, during and immediately post-exercise and the morning of each recovery day following exercise. Muscle function was determined by peak force during an isometric squat and by jump height during a static jump at pre-exercise (PRE) and 24, 48, 72, and 96 h post-exercise (24, 48, 72, 96 h). Additionally, at these time points each group's serum levels of creatine kinase (CK) and myoglobin (Mb) along with perceived feelings of muscle soreness were determined. None of the C group dependent variables was altered by the recurring testing procedures. Peak force was significantly decreased across all time points for both experimental groups. The L group experienced an attenuated drop in mean peak force across all post-exercise time points compared to the P group. Jump height significantly decreased from PRE for both the L and P group at 24 h and 48 h. CK and Mb was significantly elevated from PRE for both experimental groups at 24 h. Muscle soreness increased across all time points for the both the L and P group, and the L group experienced a significantly higher increase in mean muscle soreness post-exercise. Following exercise-induced muscle damage, high-dose leucine supplementation may help maintain force output during isometric contractions, however, not force output required for complex physical tasks thereby possibly limiting its ergogenic effectiveness.

The early rehabilitating effects of mild hypothermia for patients with herpes simplex viral encephalitis

Objective To observe the early rehabilitation effect of mild hypothermia on patients with herpes simplex viral encephalitis (HSE). Methods A total of 58 patients with HSE were randomized into two groups, a mild hypothermia therapy group (30 cases) and a normothermia control group (28 cases). Their rectal temperatures were controlled to (34±1)℃ and (37.0±0.5)℃ respectively. Serum levels of neuron specific enolase (NSE) were determined through radio-immunoassay (RIA). Soluble intercellular adhesion molecule-1 (sICAM-1) was measured with ELISA before and 1, 3, 5, and 7 d after treatment. The outcome was evaluated using the Glasgow Outcome Scale (GOS) 30 days after treatment. Results Compared with the normothermia control group, the mild hypothermia group's serum levels of NSE and sICAM-1 decreased quickly and significantly during the early stage of treatment and remained better 30 d later. Conclusion Mild hypothermia therapy can dramatically reduce inflamma-tion and facilitate the rehabilitation of damaged neurons, provide protective effects and improve the outcome for pa-tients with SHE. Key words: Herpes simplex viral encephalitis ; Mild hypothcrmia ; Neuron specific enolase ; Soluble intercellular adhesion molecule-1 ; Glasgow Outcome Scale ;