Groups LB Research Articles

Reproducibility of the new Leishmania vaccine: Changes in T1, T2 and spleen white pulp responses after re- exposure to live promastigote of Leishmania major

Introduction: Human leishmaniasis is distributed worldwide and is mainly in the tropics and subtropics, with a prevalence of 12 million cases and an approximately incidence of 0.5 million cases of VL and 1.5 million cases of cutaneous leishmaniasis (CL). Leishmania parasites are vector-born protozoan pathogens found in tropical and subtropical regions of both the old and new world. The disease in human can be divided into cutaneous, visceral, and mucosal syndromes. The aim of this study was more experiments over our previous new formulation leishmania major vaccine which had satisfactory experiments results. Method: For detail procedure refers to author’s previous publications. Briefly, hundred and twenty Balb/cmice were randomly divided into four groups as LT, LB, LBT and control groups. LT, LB and LBT injected subcutaneously with the antigen and the same booster doses with two weeks intervals. The expansion rates of the spleen white pulp size was evaluated using a light microscope and the levels of the serum Th1 (IFN- γ, IL-12) and Th2 (IL-4, IL-10) cytokines measured with the ELISA method. Results: Comparing to the LT and LB groups, the LBT group had highest levels of serum IL12, lowest levels of IL10 and highest increase in the spleen white pulp size. Significant correlation was observed between IL12 and IL10 but not IFN gamma or IL4. Conclusion: present study indicated that the LBT group which received crude cocktail leishmania antigen plus alcoholic extract of Teucrium polium and BCG as adjuvant showed satisfactory cytokines profile comparing to groups LT and LB,since highest levels of IL12 and lower levels of IL10 which could help the infected subjects to inhibit or eradication the intracellular leishmania amastigotes, and also was seen highest increase in the spleen white-pulp size which pointed to the synergistic effects of alcoholic extract of T. polium and BCG.

Fracture Resistance and Failure Mode of Endocrown Restorations with Different Remaining Walls and Finish Lines

Objective: Endocrown is a conservative treatment plan for endodontically treated teeth. The aim of this study was to evaluate the fracture resistance of four different preparation designs of endocrown restorations in mandibular molars. Methods: Thirty-six endodontically treated molars were divided into two groups (L, MD). In group L, the lingual wall and in group MD, mesial and distal walls were removed up to 1 mm above the CEJ. For each group, 2 subgroups (n=9) were considered: B) 2mm occlusal reduction with but joint finish line, S) axial reduction with radial shoulder finish line, in addition to 2mm occlusal reduction. Digital impression was performed, followed by designing and milling the lithium disilicate blocks. After cementation of the restorations and thermocycling, fracture resistance and failure mode were investigated. Results: Groups LB and LS, showed significantly higher fracture resistance compared to groups MDB and MDS (P=0.02). However, the type of finish line did not have a significant effect on the fracture resistance in the experimented groups (P=0.232). Conclusion: Unlike the finish line type, the number of remaining axial walls had a significant effect on fracture resistance. Although most of the fractures were catastrophic, they occurred due to forces much greater than usual.

Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens

Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Lactobacillus fermentum Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with Campylobacter jejuni. One-hundred and eight 1-day old COBB 500 broiler chickens were equally assigned to four experimental groups at random. In the control group (C) chicks were left untreated, whereas in groups LB and LBCj a suspension of L. fermentum was administered. A suspension of C. jejuni was subsequently applied to groups Cj and LBCj. Body weight was registered, and the individuals were later slaughtered; cecum samples were collected at 12, 36 and 48 h post-infection (hpi). The entire experiment lasted seven days. Reverse transcription quantitative PCR (RT-qPCR) was used to determine expression levels of IL-1β, IL-15, IL-17, and IL-18 at each time point. Pathogen-infected individuals were observed to weigh significantly less than those fed with the probiotic. Significant differences were also found in transcript abundance; expression of IL-15 was downregulated by the probiotic and upregulated by C. jejuni. The effects of bacterial treatments were time-dependent, as the expression profiles differed at later stages. The present outcomes demonstrate that L. fermentum both reduces the impact of C. jejuni infection on chicken body weight and regulates positively pro-inflammatory cytokine expression, which ultimately increase bird well-being and improves production.

Effect of addition of buprenorphine or dexamethasone to levobupivacaine on postoperative analgesia in ultrasound guided transversus abdominis plane block in patients undergoing unilateral inguinal hernia repair: a prospective randomized double blind controlled trial.

BackgroundThe transversus abdominis plane (TAP) block is an effective technique to block the thoracolumbar nerves innervating the anterolateral abdominal wall. This study was conducted to evaluate the analgesic efficacy and opioid consumption with the use of perineural buprenorphine or dexamethasone in TAP blocks after unilateral inguinal hernioplasties.MethodsThis prospective, randomized, double-blinded, placebo-controlled study enrolled 93 patients scheduled for unilateral inguinal hernioplasty, followed by an ultrasound-guided TAP block. The participants were randomized into 3 groups (31 patients each). Group L received 20 ml 0.25% levobupivacaine + 1 ml normal saline (NS); group LB, 20 ml 0.25% levobupivacaine + 0.3 mg (1 ml) buprenorphine; and group LD, 20 ml 0.25% levobupivacaine + 4 mg (1 ml) dexamethasone. The patients were observed postoperatively for 24 h for first rescue analgesic requirement, total rescue analgesic consumption, and pain scores on the numeric rating scale (NRS).ResultsThe time to first rescue analgesic requirement was significantly longer in Group LB than in groups LD and L (688.87 ± 36.11 min, 601.45 ± 39.85 min, and 383.06 ± 36.21 min, respectively; P < 0.001). The mean total tramadol consumption in the first 24 h was the lowest in group LB (P < 0.001, L vs. LB / LD). Groups LB and LD displayed significantly lower NRS scores than group L (P < 0.001 both).ConclusionsLevobupivacaine with perineural buprenorphine in a TAP block after unilateral open inguinal hernioplasty facilitates prolonged analgesia and reduced requirement for rescue analgesics compared to perineural dexamethasone, without significant side effects.

A comparative study of anterior cruciate ligament reconstruction with double, single, or selective bundle techniques

Reconstruction of the anterior cruciate ligament (ACL) is the most frequently performed reconstructive surgery in the knee. Biomechanical studies have shown that double bundle (DB) reconstruction is better than single bundle (SB) reconstruction with regard to rotational stability. It is postulated that resection of ACL fibres that remain in continuity may be counterproductive for the knee as these fibres have the capacity to produce collagen. In this study, we aimed to evaluate the efficacy among selective bundle, DB and SB ACL reconstructions over a 2-year post-operative follow-up period. A retrospective comparative study was conducted for comparison between selective bundle, DB and SB reconstructions. Between 2012 and 2014, 291 ACL reconstructions were performed. Of these, 68 patients had selective ACL reconstructions (group SLB), 147 had DB ACL reconstructions (group DB) and 76 had SB ACL reconstructions (group SB). Institutional Review Board approval was obtained, and all patients provided informed consent. Clinical results were assessed with the International Knee Documentation Committee (IKDC), Lysholm and Tegner scores. Stability was measured using Lachman, pivot shift and anterior drawer stress tests using the KT1000 at 30° of knee flexion. There was no significant difference in ligament grade, function grade, IKDC grade, as well as Tegner and Lysholm means among all three groups after a 2-year follow-up period. Selective bundle reconstruction provides comparable results to DB and SB reconstruction techniques. It is a viable alternative for patients with partial tears.

Th1, Th2 Serum Cytokines and Spleen White Pulp Changes Against Preliminary L. Major Vaccine Injection and Challenge With Live L. Major Promastigotes in Balb/C Mice

Introduction: Human Leishmaniasis is distributed worldwide and is mainly in the tropics and subtropics, with a prevalence of 12 million cases and an approximately incidence of 0.5 million cases of visceral Leishmaniasis(VL) and 1.5 million cases of cutaneous Leishmaniasis (CL). Leishmania parasites are vector-born protozoan pathogens found in tropical and subtropical regions of both the old and new world. The disease in human can be divided into cutaneous, visceral, and mucosal syndromes. The aim of this study was to conduct further studies over our new formulation of Leishmania major vaccine which experimentally had satisfactory results. Method: For the detail of the procedure it is referred to the author’s previous publications. Briefly one hundred and twenty Balb/c mice were randomly divided into four groups as LT, LB, LBT and control groups. Groups LT, LB and LBT injected subcutaneously with the antigen and the same booster doses with a week interval. The expansion rates of the spleen white pulp size was evaluated and the levels of the serum TH1 (IFN-γ, IL-12) and TH2 (IL-4, IL-10) cytokines measured with the ELISA method. Results: Comparing to the LT and LB groups, the LBT group had highest levels of serum IL-12, lowest levels of IL-10 and highest increase in the spleen white pulp size. Significant negative correlation was observed between IL-12 and IL-10 but not IFN-γ or IL-4. Conclusion: The present study indicated that the LBT group which received crude cocktail Leishmania antigen plus alcoholic extract of Teucrium polium and BCG as adjuvants showed satisfactory cytokines profile comparing to groups LT and LB, since highest levels of IL-12 and lower levels of IL-10 could help the infected subjects to inhibit or eradicate the intracellular Leishmania amastigotes, and also highest increase in the spleen white pulp size which pointed to the synergistic effects of BCG and alcoholic extract of T. polium.

Fornix-based versus limbal-based conjunctival flaps in trabeculectomy with mitomycin C in high-risk patients.

PurposeTo compare the surgical outcomes of limbal-based and fornix-based trabeculectomy in eyes with a history of ocular incisional surgery.MethodsTwenty-six eyes underwent limbal-based trabeculectomy (group LB), and were condition matched with 26 eyes that received fornix-based trabeculectomy (group FB). Surgical failure was recorded retrospectively if the intraocular pressure value was either ≥21, ≥18, and ≥15 mmHg (conditions A, B, and C, respectively) or <4 mmHg or if the patient required additional glaucoma surgery. Kaplan–Meier survival curve analysis was used to assess surgical failure.ResultsFor condition A, the 2 year surgical success probabilities were 75.0% and 63.9% in groups FB and LB, respectively (P=0.124). The corresponding values were 55.0% and 61.7% (P=0.638) in condition B, and 55.0% and 57.0% (P=0.454) in condition C. The rates of bleb leakage, hypotony, choroidal detachment, and bleb-related infection were 11.5%, 26.9%, 50.0%, and 7.7% in group LB, respectively. The corresponding values in group FB were 30.8%, 23.1%, 46.2%, and 0.0%, which were not statistically different between the two groups.ConclusionNo significant differences in surgical outcomes were observed between limbal-based and fornix-based trabeculectomy for patients with a history of incisional ocular surgeries.

Primary Effects of New Leishmania major Antigen on Balb/c Mice Spleen

Background: Cutaneous leishmaniasis is a zoonotic disease transmitted between rodents and canine, mainly induced by phelebotomus sand flies. In Iran, the incidence of this protozoan disease has doubled over the last decade. Regard to its high incidence, in the present study the new antigen preparation was evaluated following challenging with live Leishmania major promastigts. Methods: One hundred and twenty male and female Balb/c mice were randomly divided into four groups as LT, LB, LBT and control groups. Group LT received 100-200 μg /0.1 ml of the crude cocktail antigen preparation plus alcoholic extract of Teucrium polium as adjuvant, LB received 100-200 μg /0.1 ml of the crude cocktail antigen preparation plus BCG as adjuvant, LBT received 100-200 μg /0.1 ml of this antigen preparation plus alcoholic extract of Teucrium polium and BCG as adjuvants and control group. Groups LT, LB and LBT were injected subcutaneously with the antigen preparation and they were boosted using the same doses with two weeks intervals later. A week after the last booster, all animals was challenged with live Leishmania major promastigotes. The animals survived, were euthanized, their spleen was removed, and 4-5 microns paraffin sections were prepared and stained with Harris Hematoxylin and Eosine method. The expansion rates of the spleen white pulp size were evaluated using a light microscope with eye-piece graticule. Results & Conclusion: Statistically, Higher expansion rate of the spleen white pulp size were recorded in the LBT group. The antigen preparation supplemented with adjuvants had positive effects on spleen white pulp expansion following live Leishmania major challenging, nevertheless, based on previous experiment, 100-200 μg of LT group could induces satisfactory DTH response which favors leishmania organisms eradication, and higher survival rate after challenging with the live Leishmania major organisms in spite of induction of lower spleen white pulp size expansion.

Indirect Transmission of Influenza A Virus between Pig Populations under Two Different Biosecurity Settings

Respiratory disease due to influenza virus is common in both human and swine populations around the world with multiple transmission routes capable of transmitting influenza virus, including indirect routes. The objective of this study was to evaluate the role of fomites in influenza A virus (IAV) transmission between pig populations separated by two different biosecurity settings. Thirty-five pigs were divided into four experimental groups: 10 pigs (1 replicate) were assigned to the infected group (I), 10 pigs (2 replicates of 5 pigs) were assigned to the low biosecurity sentinel group (LB), 10 pigs (2 replicates of 5 pigs) were assigned to the medium biosecurity sentinel group (MB), and 5 pigs (1 replicate) were assigned to the negative control group (NC). Eight of 10 pigs in the infected group were inoculated with IAV and 36 hours following inoculation, personnel movement events took place in order to move potentially infectious clothing and personal protective equipment (PPE) to sentinel pig rooms. Following contact with the infected group, personnel moved to the MB group after designated hygiene measures while personnel moved directly to the LB group. Nasal swabs and blood samples were collected from pigs to assess IAV infection status and fomites were sampled and tested via RRT-PCR. All experimentally inoculated pigs were infected with IAV and 11 of the 144 fomite samples collected following contact with infected pigs were low level positive for IAV genome. One replicate of each sentinel groups LB and MB became infected with IAV and all five pigs were infected over time. This study provides evidence that fomites can serve as an IAV transmission route from infected to sentinel pigs and highlights the need to focus on indirect routes as well as direct routes of transmission for IAV.

Feasibility of landiolol and bisoprolol for prevention of atrial fibrillation after coronary artery bypass grafting: A pilot study

We previously performed a trial of intravenous landiolol hydrochloride during and after cardiac surgery (the PASCAL trial) and demonstrated a preventive effect on postoperative atrial fibrillation (AF). In the present study, we investigated the efficacy of increasing the dose and administration period of landiolol for prevention of postoperative AF, as well as the effect of oral bisoprolol in the early postoperative period. A total of 105 patients who underwent coronary artery bypass grafting were randomized to 3 groups: a group receiving intravenous landiolol perioperatively at 5 μg/kg/min for 3 days (group L), a group receiving oral bisoprolol postoperatively together with landiolol (group LB), and a control group without beta-blocker therapy (group C). The primary end point was the presence/absence of postoperative AF. Secondary end points were (1) the early clinical outcome, (2) hemodynamics, (3) cardiac enzymes (creatine kinase isoenzyme MB, troponin-I, and human heart fatty acid-binding protein), (4) high-sensitivity C-reactive protein (hs-CRP) and pentraxin-3, (5) asymmetric dimethylarginine (ADMA), and (6) brain natriuretic peptide. Postoperative AF occurred in 14.5% of group L, 9.1% of group LB, and 35.3% of group C. A significant difference was observed between groups LB and C. Significantly higher levels of troponin-I, human heart fatty acid-binding protein, hs-CRP, pentraxin-3, and ADMA were noted in group C than in groups L and LB. Landiolol and bisoprolol prevented postoperative AF. The anti-ischemic, anti-inflammatory, and anti-oxidant effects of these beta-blockers presumably inhibited the onset of AF.

Vasoconstrictive and analgesic efficacy of locally infiltrated levobupivacaine in tonsillectomy patients

ObjectivesThe use of preincisional plain levobupivacaine, lidocaine adrenaline and saline for perioperative blood loss and postoperative analgesia in pediatric tonsillectomy patients are compared. MethodsNinety patients were randomly assigned into one of the 3 groups to receive preincisional peritonsillar infiltration of levobupivacaine 0.25% (group LB), lidocaine–adrenaline 1% with 1:200,000 (group LA) and saline (group S) under general anesthesia. Intraoperative blood loss, pre- and postoperative hemoglobin (hb) and haematocrit (htc) values, hemostasis time, operation duration, number of cautery used (20W, 1s) and heart rates were recorded. Pain scores in PACU, at 6th, 12th and 24th hours postoperatively and the number of the patients requiring analgesic treatment for first 24h was also recorded. ResultsThere was a 30% reduction in perioperative blood loss in group LB and 63% reduction in group LA compared to group S (39±6, 21±4 and 55±7ml respectively) (p<0.001). Eventhough all three groups have significantly lower postoperative hb and htc values with respect to preoperative levels both local anesthetic groups had significantly higher postoperative hb and htc values than saline (p<0.001). Time required for hemostasis, the number of cautery used for haemostasis and operation duration were lower in groups LB and LA with respect to group S. Pain scores of the group LB revealed a significantly lower score throughout 24h. Only 14 patients in group LB demanded additional analgesic where as all patients in the other groups had. Adding adrenaline to the local anesthetic solution showed no side effects. Also we did not happened to see any complications related to local anesthetic injections. There was no postoperative major bleeding in groups. ConclusionLevobupivacaine has a vasoconstrictive effect in 0.25% concentrations that may be beneficial in tonsillectomy patients and has a consistent analgesic effect.

Effect of dam and sire genotypes on productive and carcass traits of rabbits1

The aim of the experiment was to study the effect of dam and sire genotypes on the production and slaughter performance of growing rabbits. The experiment was carried out at Kaposvár University. Pannon White (PW) and maternal line (M) does were inseminated with the sperm of PW, M, large body line (LB), terminal line of Hycole hybrid (H), or colored line (CL) bucks. The mature body size of the M, PW, and CL genotypes was medium. The mature body size of the LB and H genotypes was large. Only PW and LB rabbits were selected for carcass traits based on computer tomograph (CT) scanning data. The rabbits (n = 320) were weaned at the age of 5 wk and housed in a closed climate-controlled rabbitry in wire cages. Rabbits were fed ad libitum a commercial pellet. Rabbits were slaughtered at 78 d of age. Daily BW gain (5.7%, P < 0.001) and G:F (6.2%, P < 0.001) of the progeny of PW does were better than those of the progeny of the M does. The weight of the chilled carcass and body parts was greater for the progeny of the PW does (P < 0.001). The ratio of the hind part compared with the reference carcass was greater in the PW group (P < 0.001); however, the ratio of the fore part was greater in the M group (P < 0.05). Examining the effect of the genotype of the sires, the BW and daily BW gain were greater in groups H and LB, whereas the progeny of M and CL sires were inferior (P < 0.05). Body weights at 11 wk of age were H: 2,918 g, LB: 2,793 g, PW: 2,678 g, CL: 2,636 g, and M: 2,585 g (P < 0.001). Similar differences were found for daily feed intake (P < 0.05). The G:F differed (P < 0.05) significantly between groups H and CL. The weights of the chilled carcass and of the body parts were the greatest for the progeny of the H sires, and the smallest in the M group. The differences between the dressing out percentages of the groups were not significant. The ratio of the fore part was greater (P < 0.05) in groups LB, H, and CL, but the ratio of the middle part was not different. The ratio of hind part related to the reference carcass was largest in progeny of PW and least in LB and CL groups (P < 0.05). It was concluded that the production of growing rabbits was affected by the adult BW of their sires, but the carcass traits were influenced by their own adult BW and by the CT-based selection.

Comparison of 0.5% Levobupivacaine, 0.5% Bupivacaine, and 2% Lidocaine for Retrobulbar Anesthesia in Vitreoretinal Surgery

The authors compared the efficacy of local anesthetics levobupivacaine, bupivacaine, and lidocaine for retrobulbar anesthesia in vitreoretinal surgery. A total of 135 patients presenting for vitreoretinal surgery under local anesthesia were included in the study. Patients were randomly allocated to one of three groups. Group LB patients received 5 mL of 0.5% levobupivacaine, Group L patients received 5 mL of 2% lidocaine, and Group B patients received 5 mL of 0.5% bupivacaine for retrobulbar anesthesia via inferotemporal injection. Sensory and motor block durations were recorded. Intraoperative and postoperative pain was assessed by using verbal pain scala. Anesthesia efficiency, patient and surgeon satisfaction, and akinesia were assessed by using point scales. Hemodynamic data and adverse events were recorded. The demographic characteristics of patients, duration of surgery, and hemodynamic data in both groups were similar. The duration of motor and sensory block was longer in levobupivacaine and bupivacaine groups than lidocaine group. Pain on injection was found more frequent in Group L and Group B than Group LB and the difference between the Groups LB and B was significant (p<0.05). Surgeon and patient satisfaction were also higher and intraoperative pain was less in levobupivacaine group than lidocaine and bupivacaine groups. Levobupivacaine provides longer motor and sensory block duration and higher surgeon and patient satisfaction than lidocaine and bupivacaine when used for retrobulbar anesthesia in vitreoretinal surgery.

Alkalinization of Intracuff Lidocaine Improves Endotracheal Tube-Induced Emergence Phenomena

We sought to evaluate the effect of filling an endotracheal tube cuff with 40 mg lidocaine alone (Group L) or alkalinized lidocaine (Group LB) in comparison to an Air Control group (Group C) on adverse emergence phenomena in a randomized controlled study (n = 25 in each group). The incidence of sore throat was decreased for Group LB in comparison to Group L during the 24 postextubation hours. The difference between Group L and Group C remained significant in the two postextubation hours only. Plasma lidocaine levels increased when lidocaine was alkalinized (Cmax were 62.5 ± 34.0 ng/mL and 3.2 ± 1.0 ng/mL for Groups LB and L, respectively). Cough and restlessness before tracheal extubation were decreased in Group LB compared with Group L and in Group L compared with Group C. Nausea, postoperative vomiting, dysphonia, and hoarseness were increased after extubation in Group C compared with the liquid groups, and a better tolerance was recorded with Group LB compared with Group L. The increase of arterial blood pressure and cardiac frequencies during the extubation period was less in the liquid groups than in the control group and less in Group LB compared with Group L. We concluded that use of intracuff alkalinized lidocaine is an effective adjunct to endotracheal intubation.

KONTRIBUSI KOMPETENSI DAN LINGKUNGAN KERJA TERHADAP KINERJA GURU

This research is motivated by the low performance of teachers, aims to examine descriptively about competence, work environment, and teacher performance and examine verification about the effect of competence and environment on teacher performance both partially and simultaneously. The research method used is descriptive and verification. The population consisted of 51 Sumedang Group SLB teachers with a proportional stratified random sampling technique. Data sources in this study are primary and secondary data with correlation and regression analysis techniques. Descriptive research results prove that teacher competence and the work environment are in good condition, but there are still weaknesses in certain aspects. Verification analysis results prove that teacher competence and work environment influence teacher performance both partially and simultaneously.