SM Group Research Articles

Effect of different genetic backgrounds on rumen microbiota and serum metabolic phenotypes in beef cattle

Species with different genetic backgrounds exhibit distinct metabolic traits. Nine beef cattle were selected for the experiment to study changes in serum metabolic phenotypes, rumen microbiota diversity, and composition in beef cattle from different genetic backgrounds. Three groups were Chinese Simmental (S group), Simmental×Chinese Holstein (SH group), and Simmental × Mongolian (SM group) cattle. We used ELISA to detect serum biochemical indicators. The Short-chain fatty acids (SCFAs) in the rumen were examined, and a significant difference was observed in the acetic acid content of the three experimental groups (p < 0.01). The propionic acid content in the rumen of the S group was significantly higher than that of the SH and SM groups (p < 0.05). The A/P ratios of both the S and SM groups were significantly higher than that of the SH group (p < 0.05). We analyzed rumen microbiota composition and diversity in each group of cattle using 16 S rRNA sequencing and found that their composition was generally similar in the three groups of crossbred fattening cattle; however, the f_Bacteroidales_RF16_group and g_norank_f_Bacteroidales_RF16_group were significantly enriched in the SH group, whereas Treponema and Spirochaetia were significantly enriched in the SM group. Spirochaetia was significantly enriched in the SM group. Differences in rumen bacterial enrichment indicated that starch, protein, and cellulolytic abilities differed among the S, SH, and SM groups. The results of Spearman correlation analysis confirmed the correlation between rumen genera and serum biochemical indices. Overall, differences in rumen microflora play an important role in influencing the serum metabolic phenotype.

Effects of Interactions between Feeding Patterns and the Gut Microbiota on Pig Reproductive Performance.

The feeding mode is an important factor affecting the reproductive performance of pigs. The composition and expression of the intestinal microbiota are closely related to the physiological and biochemical indicators of animals. Therefore, to explore the impact of different feeding patterns on the reproductive performance of pigs, this study collected reproductive performance data from 1607 Yorkshire pigs raised under different feeding patterns and conducted a fixed-effect variance analysis. Among them, 731 were in the artificial feeding (AM) group and 876 were in the feeding station feeding (SM) group. Additionally, 40 Yorkshire sows in the late gestation period were randomly selected from each feeding mode for intestinal microbiota analysis. The results of the analysis showed that, in the AM group, both the number of birth deformities (NBD) and the number of stillbirths (NSB) were significantly greater than they were in the SM group (p < 0.05). Additionally, the total number born (TNB) in the AM group was significantly lower than that in the SM group (p < 0.05). The results of the intestinal microbiota analysis revealed that at the phylum level, there were significant differences in nine bacterial taxa between the AM and SM groups (p < 0.05). At the genus level, the abundance of a variety of beneficial bacteria related to reproductive performance in the SM group was significantly greater than that in the AM group. Finally, fecal metabolomic analysis revealed that the contents of butyric acid, isovaleric acid, valeric acid, and isobutyric acid, which are associated with reproductive performance, in the feces of sows in the SM group were significantly higher than those in the AM group (p < 0.05). These results indicate that different feeding methods can affect the gut microbiota composition of Yorkshire pigs and further influence the reproductive performance of pigs through the gut microbiota-metabolic product pathway. The results of this study provide valuable insights for further exploring the relationships between feeding modes, intestinal microbial composition, and host phenotypes.

Seminormal forms for the Temperley-Lieb algebra

Let TLnQ be the rational Temperley-Lieb algebra, with loop parameter 2. In the first part of the paper we study the seminormal idempotents Et for TLnQ for t running over two-column standard tableaux. Our main result is here a concrete combinatorial construction of Et using Jones-Wenzl idempotents JWk for TLkQ where k≤n.In the second part of the paper we consider the Temperley-Lieb algebra TLnFp over the finite field Fp, where p>2. The KLR-approach to TLnFp gives rise to an action of a symmetric group Sm on TLnFp, for some m<n. We show that the Et's from the first part of the paper are simultaneous eigenvectors for the associated Jucys-Murphy elements for Sm. This leads to a KLR-interpretation of the p-Jones-Wenzl idempotent JWnp for TLnFp, that was introduced recently by Burull, Libedinsky and Sentinelli.

Structural architecture and metamorphism of the Mayombe Chain and Niari Basin (West Congo Belt) in Congo Brazzaville

Geological mapping of the Mayombe Chain and Niari Basin of Congo Brazzaville allows for the first time defining the structural architecture and metamorphism of the West Congo Belt. Four different tectono-metamorphic domains, separated by crustal-scale shear zones, are now distinguished (Niari Basin (NB), Eastern (EMC), Central (CMC) and Western (WMC) Mayombe Chain).The NB is marked by only weak regional deformation under middle to upper diagenetic conditions. It is delimited in the west from the EMC by the Mount Belo Shear Zone forming the terminal thrust system of the West Congo orogen.The tectonic style in the EMC is characterized by discrete, widely-spaced low-angle thrusts, reverse faults and strike-slip faults resulting in the formation of duplex and/or positive flower structures. Off these high-strain zones, the rocks are folded into gentle syn- and anticlines. Penetrative schistosity starts in shales in the western part. The metamorphism increases from eastern anchizonal conditions to lower greenschist facies in the west. The EMC is juxtaposed along the Moukondo thrust/back-thrust system with the CMC.The CMC is typified by open to closed upright to NE-verging folds, S1 schistosity with moderate to steep SW dips, onset of regional crenulation cleavage (S2), frequent reverse thrusts and numerous faults. Metamorphic conditions remain in the greenschist facies. The Loukenéné-Mandji Thrust marks the CMC-WMC contact and coincides with a jump in metamorphic grade marked by biotite-in.The WMC consists of Palaeoproterozoic basement stacked with Neoproterozoic rocks. Autochthonous Palaeoproterozoic gneiss and schist record Late Eburnean sedimentation, magmatism and metamorphism between 2110 and 1970 Ma, which are compared with the Eburnean history in Gabon and the Transamazonian orogeny in Brazil. The allochthonous Bikossi Group was thrust during the Pan-African event from the west over Tonian metavolcaniclastic and plutonic rocks before further folding and stacking of both units. The intensity of Pan-African deformation increases from open to closed folds with spaced cleavage in the southeast of the WMC to thrust-dominated tectonics in the northwest, where the Palaeo- and Neoproterozoic rocks are transposed into parallelism with the pronounced schistosity.Geochronology of illite and muscovite documents two Pan-African events at 590-570 Ma (M1) and at 520-500 Ma (M2) that are related to the main collisional and late thermal events in the Araçuai-Ribeira Belt in Brazil. Metamorphic isogrades shifted from M1 to M2 for more than 30 km to the west. Detrital mica and metamorphic illite of the Mpioka Group record M1 and M2, respectively constraining sedimentary deposition between 570 and 520 Ma, which implies the interpretation of the group as molasse of the West Congo Belt.

Sanhua Tang protects against ischemic stroke by preventing blood-brain barrier injury: a network pharmacology and experiments.

To assess the effect and mechanism of Sanhua Tang (, SHT) in treating ischemic stroke (IS) through the "Kaitong Xuanfu" theory by using network pharmacology and animal experiments. The active ingredients and targets of SHT and IS were screened by public databases such as Traditional Chinese Medicine systems pharmacology, GeneCards, and online mendelian inheritance in man. Visual network topographies were constructed using R, Cytoscape 3.6.0, AutoDockTools, a user-sponsored molecular visualization system on an open-source foundation, and other software to analyze the correlation between targets and active ingredients. The middle cerebral artery occlusion (MCAO) model was established by operation. Animals were divided into the Sham group, MCAO group (M group), aloe-emodin (AE) group (MCAO rats treated with aloe-emodin), SHT at low dosage (SL group) (MCAO rats treated with SL), SHT at medium dosage (SM group), and SHT at high dosage (SH group). 2,3,5-triphenyl tetrazolium chloride staining was used to detect the volume of cerebral infarction; Nissl staining was used to observe the morphology of neuronal cells; transmission electron microscopy was used to observe the integrity of the blood-brain barrier (BBB); enzyme-linked immunosorbent assay was used to detect the content of interleukin-6 (IL-6), IL-10, tumor necrosis factor α (TNF-α) in serum. Western blot was used to detect the expression of vascular endothelial growth factor A (VEGFA) protein in the cerebral ischemic penumbra. Using network pharmacology and molecular docking validation, four active ingredients (lignan, naringenin, aloe-rhodopsin, and β-sitosterol), seven target proteins (protein kinase b 1, IL-6, TNF, VEGFA, TP53, jun proto-oncogene, and cysteinyl aspartate specific proteinase 3), and inflammatory signaling pathways were identified. Animal experiments showed that the SH and AE groups had fewer neurological deficits, reduced brain infarct volumes, decreased serum inflammatory factor levels, increased expression of VEGFA protein, and less structural damage to neurons and BBB. The present study found that the therapeutic mechanism of SHT against IS may be related to the inhibition of BBB inflammatory damage, which is also the mechanism of "Kaitong Xuanfu." The high-dose group of SHT was relatively effective in regulating inflammatory factors, improving BBB permeability, and protecting neuronal cells from damage.

Evaluation of fracture resistance and marginal fit of implant-supported interim crowns fabricated by conventional, additive and subtractive methods

BackgroundInterim crowns are utilized for restoring implants during and after the process of osseointegration. However, studies on adaptation and fracture strength of implant-supported interim crowns are rare.Aim of the studyThe aim of this in vitro study is evaluating marginal fit and fracture resistance of conventional, subtractive, and additive methods of fabricating implant-supported interim crowns.Materials and methodsAn implant was placed in an epoxy resin model with a missing first molar. A scan body was attached, and scanned with an intraoral scanner (IOS), the STL file was used to fabricate eighteen master models with standardized implant digital analogue spaces. The digital analogues and their corresponding abutments were attached to the master models and scanned with the IOS, the STL files were used to fabricate eighteen crowns using three different techniques (n = 6): conventional (CR); from Autopolymerizing composite resin, subtractive (SM); milled from PMMA resin blanks, and additive (AM); from 3D printed resin material. The crowns were fitted and cemented on their corresponding abutments and subjected to cyclic loading and thermocycling. The marginal fit was evaluated using a stereomicroscope. The crowns were then loaded until fractured in a universal testing machine. The Shapiro–Wilk and the Kolmogorov-Smirnov tests revealed that data of Marginal gap was non-parametric. Kruskal-Wallis test followed by the Dunn test was used (α = 0.05). While data of Fracture resistance test was parametric. ANOVA (F-test) was used followed by the Tukey test (α = 0.05).ResultsFor marginal gap, a significant difference was shown between the study groups (P = .001) according to Kruskal–Wallis test. Groups SM and AM had significantly lower marginal gap values compared to group CR (P = .003). No significant difference was found between groups SM and AM (P = .994). For fracture resistance, One-way ANOVA revealed a significant difference in fracture resistance between study groups (P < .001). Group SM had significantly higher fracture strength followed by group AM and group CR (P = .001).ConclusionsGroup SM and AM showed better marginal adaptation than group CR. Group SM showed superior fracture resistance compared to other groups. All study groups showed acceptable marginal gap and fracture resistance.

Dietary vitamin E further enhances the effectiveness of Clostridium autoethanogenum protein as an alternative source of soybean meal in grass carp (Ctenopharygodon idellus) diets based on growth performance, flesh quality and antioxidant status

The effects of replacing soybean meal with Clostridium autoethanogenum protein and supplementing it with different gradients of vitamin E in grass carp diets on growth performance, flesh quality and antioxidant capacity were evaluated in a 69-day feeding trial. Five isonitrogen and isolipid diets were configured with the soybean meal-based diet (SM) served as the basal control diet, and the Clostridium autoethanogenum protein-based diet (CAP) as the treatment control diet. The CAP diet was supplemented with 80 mg/kg, 160 mg/kg and 240 mg/kg vitamin E (namely, 80VE, 160VE and 240VE), respectively. Final body weight (FBW) and specific growth rate (SGR) were significantly better in the CAP group than in the SM group (P < 0.05), and the 160 VE group was significantly better than the CAP group (P < 0.05). In terms of muscle quality, muscle fat content was significantly higher in the CAP group than in the SM group (P < 0.05), and muscle vitamin E content was significantly higher in each vitamin E-supplemented group than in the CAP group (P > 0.05). The total odor activity value in muscle in the CAP group was not significantly different from the SM group (P < 0.05), but the vitamin E-supplemented groups were significantly higher than the CAP group (P < 0.05). Muscle hardness and gumminess were significantly higher in the CAP group than the SM group and muscle shear force and gumminess were significantly higher in the 80VE group than the CAP group (P < 0.05). Muscle development-related genes in the CAP group were not significantly different from those in the SM group (P > 0.05), and Muscle Myf5 mRNA levels in the 160VE and 240VE groups were significantly higher than those in the CAP group, while the expression of MSTN genes was suppressed (P < 0.05). In terms of antioxidant properties, muscle MDA content was significantly higher in the CAP group than in the SM group (P < 0.05), and muscle MDA content was significantly lower in the 160VE and 240VE groups than in the CAP group (P < 0.05), at the same time, CAT and T-SOD activities were significantly higher (P < 0.05). In conclusion, complete replacement of soybean meal by the Clostridium autoethanogenum protein in grass carp diets promoted growth performance and muscle quality, but negatively affected antioxidant capacity, and supplementation of 160 mg/kg vitamin E in the CAP diet could further enhance growth performance and improve antioxidant capacity.

Immobilization of Heavy Metals by Phosphorus-solubilizing Bacteria and Inhibition of Cd and Pb Uptake by Wheat

Phosphorus-solubilizing microorganisms convert insoluble phosphorus in the soil into phosphorus that can be absorbed by plants. Soluble phosphate combines with heavy metals to form precipitation, reducing the content of available heavy metals, thereby reducing the absorption of heavy metals by crops, which plays an important role in the remediation of heavy metal-contaminated soil. The effects of the immobilization of Cd and Pb and the release of PO43- by the phosphorus-solubilizing bacterium Klebsiella sp. M2 were studied through solution culture experiments. In addition, the effects of strain M2 on wheat uptake of Cd and Pb and its microbiological mechanism were also explored through pot experiments. The results showed that strain M2 reduced the concentrations of Cd and Pb and increased the concentration of PO43- in the solution through cell wall adsorption and induced phosphate precipitation. Pot experiments showed that compared to those in the CK group and inactivated strain M2 group, inoculation with live strain M2 significantly increased （123%-293%） the contents of Ca2-P and Ca8-P in rhizosphere soil, decreased the content of DTPA-Cd （34.48%） and DTPA-Pb （36.72%） in wheat rhizosphere soil, and thus hindered the accumulation of Cd and Pb in wheat grains. Moreover, high-throughput sequencing results showed that strain M2 significantly increased the diversity of wheat rhizosphere bacterial communities； increased the relative abundance of Proteobacteria, Gemmatimonadetes, and Bacteroidota in wheat rhizosphere soil； and increased the proportion of heavy metal-immobilizing and phosphorus-promoting bacteria in wheat rhizosphere soil （mainly Sphingomonas, Nocardioides, Bacillus, Gemmatimonas, and Enterobacter）. These bacterial genera played an important role in immobilizing heavy metals and preventing wheat from absorbing heavy metals. These results provide bacterial resources and theoretical basis for the bioremediation of heavy metal-contaminated farmland.

Expression and Prognostic Value of a Novel B7-H3 (CD276) Antibody in Acute Myeloid Leukemia.

Despite recent advances in immunophenotyping, the prognosis of acute myeloid leukemia (AML) is still mainly estimated using age and genetic markers. As the genetic heterogeneity of AML patients is high, flow cytometry-based classification with appropriate biomarkers can efficiently complement risk stratification and treatment selection. An increased expression of B7-H3 (CD276), an immune checkpoint protein, has been reported and associated with poor prognosis. However, the available data are limited and heterogeneous. Here, we used a novel, proprietary murine anti-B7-H3 8H8 antibody for the flow cytometric analysis of B7-H3 expression in AML blasts from 77 patients. Our antibody reliably detected substantial B7-H3 expression in 62.3% of AML patients. B7-H3 expression was higher in the monocytic French-American-British (FAB) M5 group and in intermediate and poor risk patients according to the European Leukemia Network. Using receiver operating characteristics (ROCs), we identified a specific fluorescence intensity cut-off of 4.45 to discriminate between B7-H3high and B7-H3low expression. High B7-H3 expression was associated with shorter overall survival (OS) and progression-free survival (PFS). In conclusion, we have developed a novel B7-H3 antibody that serves as a new tool for the detection of B7-H3 expression in AML and may help to facilitate risk stratification and treatment selection in AML patients.

Modified peroral endoscopic myotomy technique with retrograde on-demand myotomy for achalasia: a retrospective cohort study (with video).

Although myotomy is crucial in peroral endoscopic myotomy (POEM) surgeries, its optimum length remains controversial. Herein, we propose a modified POEM with new method of tailoring myotomy length aim to evaluate the safety, efficacy, and clinical outcomes of this modified POEM compared with standard POEM in type I or II achalasia. Seventy-five patients with type I or II achalasia who underwent POEM at the First Hospital of Jilin University between January 2018 and December 2022 were retrospectively analyzed. According to the myotomy approach, these patients were divided into the retrograde on-demand myotomy (RDM, n = 34), with myotomy beginning on gastric side and length tailored by determining the degree of lower esophageal sphincter (LES) distention, and standard myotomy (SM, n = 41) groups. The baseline data, myotomy length, operation time, clinical success rate, adverse event rate, and reflux-related adverse events were compared and analyzed. The median myotomy length in the RDM group was significantly shorter than that in the SM group (6 vs. 8cm, respectively; p < 0.001). Moreover, the median myotomy time in the RDM group was significantly shorter than that in the SM group (10 vs. 16min, respectively; p < 0.001). POEM was successfully performed in all the patients. At the 2-year follow-up, high clinical success rates were observed in both the RDM and SM groups (92.0% vs. 93.3%, respectively; p = 1.000). The incidence of intraoperative adverse events and postoperative reflux-related adverse events was low and comparable in both groups. RDM POEM is a safe and effective method for patients with type I or II achalasia. Furthermore, it has a shorter myotomy length and operation time than standard POEM technique.

The effect of different implant-abutmenttypes and heights on screw loosening in cases with increased crown height space.

The stability of the abutment screw is pivotal for successful implant-supported restorations, yet screw loosening remains a common complication, leading to compromised function and potential implant failure. This study aims to evaluate the effect of different implant-abutment types and heights on screw loosening in cases with increased crown height space (CHS). In this in vitro study, a total of 64 abutments in eight distinct groups based on their type and height were evaluated. These groups included stock, cast, and milled abutments with heights of 4 mm (groups S4, C4, and M4), 7 mm (groups S7, C7, and M7), and 10 mm (groups C10 and M10). Removal torque loss (RTL) was assessed both before and after subjecting the abutments to dynamic cyclic loading. Additionally, the differences between initial RTL and RTL following cyclic loading were analyzed for each group (p < .05). The C10 group demonstrated the highest RTL, whereas the S4 group exhibited the lowest initial RTL percentage (p < .05). Furthermore, the study established significant variations in RTL percentages and the discrepancies between initial and postcyclic loading RTL across different abutment groups (p < .05). Additionally, both abutment types and heights were found to significantly influence the RTL percentage (p < .05). The type and height of the implant abutment affected screw loosening, and in an increased CHS of 12 mm, using a stock abutment with a postheight of 4 mm can be effective in minimizing screw loosening.

TBX3 transfection and nodal signal pathway inhibition promote differentiation of adipose mesenchymal stem cell to cardiac pacemaker-like cells.

Mesenchymal stem cells (MSCs) are known as one of the best candidate cells to produce cardiac pacemaker-like cells (CPLCs). Upregulation of TBX3 transcription factor and inhibition of the nodal signal pathway have a significant role in the formation of cardiac pacemaker cells such as sinoatrial and atrioventricular nodes, which initiate the heartbeat and control the rhythm of heart contractions. This study aimed to confirm the effects of transfection of TBX3 transcription factor and inhibition of the nodal signal pathway on differentiating adipose-derived MSCs (AD-MSCs) to CPLCs. AD-MSCs were characterized using flow cytometry and three-lineage differentiation staining. The transfection of TBX3 plasmid was carried out using lipofectamine, and inhibition of the nodal signal pathway was done using the small-molecule SB431542. The morphology of the cells was observed using a light microscope. Pacemaker-specific markers, including TBX3, Cx30, HCN4, HCN1, HCN3, and KCNN4, were evaluated using the qRT-PCR method. For protein level, TBX3 and Cx30 were evaluated using ELISA and immunofluorescence staining. The electrophysiology of cells was evaluated using a patch clamp. The TBX3 expression in the TBX3, SM, and TBX + SM groups significantly higher (p < 0.05) compared to the control group and cardiomyocytes. The expression of Cx40 and Cx43 genes were lower in TBX3, SM, TBX + SM groups. In contrast, Cx30 gene showed higher expression in TBX3 group. The expression HCN1, HCN3, and HCN4 genes are higher in TBX3 group. The transfection of TBX3 and inhibition of the nodal signal pathway by small-molecule SB431542 enhanced differentiation of AD-MSCs to CPLCs.

Effect of three oral pathogens on the TMA-TMAO metabolic pathway.

Trimethylamine-N-oxide (TMAO) is produced by hepatic flavin-containing monooxygenase 3 (FMO3) from trimethylamine (TMA). High TMAO level is a biomarker of cardiovascular diseases and metabolic disorders, and it also affects periodontitis through interactions with the gastrointestinal microbiome. While recent findings indicate that periodontitis may alter systemic TMAO levels, the specific mechanisms linking these changes and particular oral pathogens require further clarification. In this study, we established a C57BL/6J male mouse model by orally administering Porphyromonas gingivalis (P. gingivalis, Pg), Fusobacterium nucleatum (F. nucleatum, Fn), Streptococcus mutans (S. mutans, Sm) and PBS was used as a control. We conducted LC-MS/MS analysis to quantify the concentrations of TMAO and its precursors in the plasma and cecal contents of mice. The diversity and composition of the gut microbiome were analyzed using 16S rRNA sequencing. TMAO-related lipid metabolism and enzymes in the intestines and liver were assessed by qPCR and ELISA methods. We further explored the effect of Pg on FMO3 expression and lipid molecules in HepG2 cells by stimulating the cells with Pg-LPS in vitro. The three oral pathogenic bacteria were orally administered to the mice for 5 weeks. The Pg group showed a marked increase in plasma TMAO, betaine, and creatinine levels, whereas no significant differences were observed in the gut TMAO level among the four groups. Further analysis showed similar diversity and composition in the gut microbiomes of both the Pg and Fn groups, which were different from the Sm and control groups. The profiles of TMA-TMAO pathway-related genera and gut enzymes were not significantly different among all groups. The Pg group showed significantly higher liver FMO3 levels and elevated lipid factors (IL-6, TG, TC, and NEFA) in contrast to the other groups. In vitro experiments confirmed that stimulation of HepG2 cells with Pg-LPS upregulated the expression of FMO3 and increased the lipid factors TC, TG, and IL-6. This study conclusively demonstrates that Pg, compared to Fn and Sm, plays a critical role in elevating plasma TMAO levels and significantly influences the TMA-TMAO pathway, primarily by modulating the expression of hepatic FMO3 and directly impacting hepatic lipid metabolism.

Association of myopia and astigmatism with postoperative ocular high order aberration after small incision lenticule extraction

ObjectiveTo investigate the correlation between higher-order aberrations (HOA) after small incision lenticule extraction (SMILE) and the severity of myopia and astigmatism, along with the relevant factors. These findings will provide valuable insights for decreasing the occurrence of HOA after SMILE and enhancing visual quality.MethodsA total of 75 patients (150 eyes) with myopia and astigmatism who underwent SMILE were categorized into four groups based on the severity of myopia and astigmatism: Myopia Group 1 (Group M1, spherical diopter ranged from -1.00 D to -4.00 D), Myopia Group 2 (Group M2, spherical diopter ranged from -4.10 D to -10.00 D), Astigmatism Group 1 (Group A1, cylindrical diopter ranged from 0 D to -1.00 D), and Astigmatism Group 2 (Group A2, cylindrical diopter ranged from -1.10 D to -3.00 D). A comprehensive assessment was performed to examine the association between HOA and various relevant factors, including a detailed analysis of the subgroups.ResultsGroup M1 had significantly lower levels of total eye coma aberration (CA), corneal total HOA (tHOA), internal tHOA, and vertical CA (Z3-1\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${{\ ext{Z}}}_{3}^{-1}$$\\end{document}) after SMILE than Group M2 (P < 0.05). Similarly, Group A1 had significantly lower levels of total eye tHOA, CA, trefoil aberration (TA), corneal tHOA, TA, and vertical TA (Z3-3\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${{\ ext{Z}}}_{3}^{-3}$$\\end{document}) after SMILE than Group A2 (P < 0.05). Pearson correlation analysis indicated a statistically significant positive relationship between the severity of myopia/astigmatism and most HOA (P < 0.05). Subgroup evaluations demonstrated a notable increase in postoperative HOA associated with myopia and astigmatism in Groups M2 and A2 compared with the control group. Lenticule thickness, postoperative central corneal thickness (CCT), postoperative uncorrected distance visual acuity (UDVA), and postoperative corneal Km and Cyl were strongly correlated with most HOA. Age, eyes, and postoperative intraocular pressure (IOP) were only associated with specific HOA.ConclusionHOA positively correlated with the severity of myopia and astigmatism after SMILE. However, this relationship was not linear. HOA after SMILE was influenced by various factors, and additional specialized investigations are required to establish its clinical importance.

Mannan oligosaccharide improves antioxidant capacity, non-specific immunity and protection against Vibrio disease and Typhoon stress in Trachinotus ovatus juveniles

This study examined the impact of varying doses of mannan oligosaccharide (MOS) supplementation on antioxidant defense, non-specific immunity, resistance to typhoon stress, and resistance to disease in Trachinotus ovatus. Fish groups C, M1, M2, and M3 were fed 1%, 0.3%, 0.6%, and 1% MOS-supplemented feed, respectively. On the 28th and 56th day of the feeding trial, samples from all groups were taken to measure the antioxidant and non-specific immunity parameters and challenged with Vibrio harvey. On the 32nd day, a typhoon hit the experimental site, and the survival rates of all groups were calculated. Results showed that on the 28th day, groups M2 and M3 showed considerably higher levels of antioxidant abilities (T-AOC), and groups M1 and M2 showed significantly enhanced non-specific immunity (AKP, LZM, and TP) levels than those of group C (p &lt; 0.05). All MOS groups had significantly lower cumulative mortality following the challenge test and typhoon stress than the control group (p &lt; 0.05). On the 56th day, groups M2 and M3 showed significantly enhanced antioxidant ability (CAT) compared to the control group. Only one non-specific immunity parameter increased significantly in groups M2 and M3 (p &lt; 0.05). After the challenge test, only group M3 exhibited a significantly declined cumulative mortality rate compared to the control groups (p &lt; 0.05). In summary, supplementation of MOS at dose 0.6% (M2) for 28 days showed the best overall improving effects on the antioxidant capacity, non-specific immunity, disease resistance, and typhoon stress resistance in T. ovatus juveniles.

Helminth-induced impairment of humoral immunity differently contribute to their anti-arthritic effects in mice: Comparison of Schistosoma mansoni and Trichinella spiralis

AimsWe have previously reported reduction of anti-type II collagen (IIC) IgG levels in collagen-induced arthritis (CIA) by Schistosoma mansoni (Sm) and Trichinella spiralis (Ts). To clarify the contribution of the impairment of humoral immunity to their anti-arthritic activities, we herein investigated the relationship between anti-IIC IgG levels and arthritic swelling in Sm- or Ts-infected mice. Methods and resultsMale DBA/1J mice were infected with Sm cercariae or Ts muscle larvae prior to the IIC immunization. In the Sm-infected mice, paw swelling and anti-IIC IgG levels were continuously lower than those of non-infected control group. In contrast, arthritic swelling in the Ts-infected mice only decreased in the early phase of CIA progression, despite the continued impairment of anti-IIC IgG production throughout the experimental period. Correlation coefficients between residual paw swelling and anti-IIC IgG titers were similar or higher in the Sm group than in the control group, but were similar or lower in the Ts group than in the control group. ConclusionThe down-modulations of anti-IIC IgG levels by the two parasitic infections and the correlation analyses suggest that the anti-arthritic activity of Sm was primarily attributed to the modulation of IgG-independent arthritogenic mechanisms and secondarily to the impairment of anti-IIC IgG production. In contrast, Ts could alleviate CIA mainly via the impairment of antibody production.

Effect of oral dosage of Spirulina platensis and silver nanoparticles on glucose, lipid profile, and liver enzymes in male rats induced di-abetics by alloxan

Diabetes is believed to be one of the most important challenges that are facing societies around the world. The research was conducted to determine the effectiveness of 150 mg/ml of Spirulina platensis and 10 mg/ml of silver nanoparticles Ag-NPs on the sugar level, lipid profile, and liver enzyme concentration in male rats. Diabetes was induced with alloxan and bred for four weeks. The rats were divided randomly into five groups and every single group contained five rats; M1 was the control group, M2 was a treated diabetic rat, M3 treated diabetic rats with 10 mg/ml Ag-NPs, M4 treated diabetic rats with 150 mg/ml S. platensis and M5 group with diabetic rats that were treated with 10 mg/ml Ag-NPs + 150 mg/ml S. platensis. The results showed that the group of rats infected with Alloxan (M2) caused negative effects at a significant level (P&lt;0.05) on the blood sugar level, while the group of rats treated with Ag-NPs (M3) or S. platensis (M4) or both (M5) showed a positive effect on the blood glucose rate, which reached 292.6, 210.5, and 199.3 mg/dl, respectively, compared to the (M2) group, which was 441.8 mg/dl. The results of the lipids profile, the group with diabetes (M2) showed an increase in the level of triglycerides (TG), cholesterol (TC), low-density lipoproteins (LDL), and very low-density lipoproteins (VLDL), and a decrease in the level of high-density lipoproteins (HDL). The treatment groups M3, M4, and M5 improved significantly positive blood lipid levels. The same situation applied to the liver enzymes AST, ALT, and ALP, whose values decreased significantly in the treatment groups (M3, M4, and M5) compared to the infection group (M2).&#x0D;

Impact of rituximab treatment regime on time to relapse in aquaporin-4 antibody positive neuromyelitis optica spectrum disorder

BackgroundAquaporin-4 (AQP4) antibody associated neuromyelitis optica (NMOSD) requires long-term immunosuppression. Rituximab is increasingly used worldwide, however the optimal regime is not established. MethodsWe retrospectively examined different rituximab regimens in AQP4-NMOSD. Standard monotherapy (SM; 6 monthly infusions), SM plus oral steroids (SM+S), extended interval dosing (EID; guided by CD19 repopulation) and EID with oral steroids (EID+S) were compared. The primary outcome was time to first clinical relapse. Potential confounders including age, gender, number of previous relapses, and onset phenotype were included. Results77 patients were included: 67 females, median onset age 35.6, median DSS at rituximab initiation 5.0. 39 were on SM+S, 20 SM, 6 EID, and 12 EID+S. 25/77 patients relapsed during a median follow-up of 44.0 months. No significant difference in time to first relapse was observed between any rituximab regimen. Pooled analyses to compare regimens that use standard monotherapy (SM and SM+S) against those that use extended interval dosing (EID and EID+S) showed no significant difference. Pooled analysis of regimens using steroids with those not using steroids also showed no significant difference. Adjusted Cox proportional hazard model revealed no significant difference between rituximab regimens or influence of demographic factors. 9 significant adverse events were recorded, 5 in the SM group and 4 in SM+S. ConclusionsThis study provides some basis for further exploring EID as a viable option for long term treatment of AQP4-NMOSD. This may improve patient experience and consolidate use of hospital resources.

Establishment of acute graft-versus-host disease model after non-myeloablative haploidentical peripheral blood stem cell transplantation in aged mice

Objective: To establish an acute graft-versus-host disease (aGVHD) model in aged mice after non-myeloablative haploidentical peripheral blood stem cell transplantation (haplo-PSCT). Methods: C57BL/6 (H-2b) male mice aged 6-8 weeks were used as donor mice, and CB6F1 (H-2b×d) female mice aged 14-16 months were used as recipient mice. The donor mice were injected subcutaneously with rehuman granulocyte-colony stimulating factor (rhG-CSF) 5 days before transplantation for hematopoietic stem cell mobilization.The recipient mice were divided into control group (CG), spleen cell low-dose group (SL), spleen cell medium-dose group (SM) and spleen cell high-dose group (SH) according to random number table method, with 16 rats in each group, all of which received total linear accelerator X-ray irradiation (TBI) with a total dose of 6 Gy. Peripheral blood mononuclear cells (PBMC) and spleen cells of different doses (0.5×107/each, 1.0×107/each and 2.0×107/each in SL group, SM group and SH group, respectively) were transfused through the tail vein within 4 hours after TBI, and only the same amount of normal saline was transfused in CG group. After transplantation, the survival and weight changes of mice in each group were observed for 30 days, and the changes of blood routine were monitored regularly. Mice peripheral blood was collected 21 days after transplantation to detect the chimerism rate of the donor. Hematoxylin-eosin staining was performed on the skin, liver and colon of mice 21 days after transplantation to analyze the histopathological changes of aGVHD target organs. Results: All the mice in each group were successfully transplanted. After TBI, the weight and activity of mice in all groups decreased, and the phenomenon of bone marrow suppression appeared. During the observation period, all mice in CG group and SL group survived, 3 mice in SM group died with survival time of (26.0±5.8) days, and 6 mice in SH group died with survival time of (20.9±7.3) days. The body weight of mice in SH group was lower than that in CG group, SL group and SM group 21days after transplantation [(25.0±0.7), (25.5±0.4), (25.0±1.4) vs (20.8±0.8) g, all P<0.05]. Compared with CG group, SL group and SM group, the levels of leukocyte, erythrocyte, hemoglobin and platelet in SH group decreased 21 days after transplantation (all P<0.05). There was no significant difference in donor chimerism rate among SL group, SM group and SH group [(95.8%±0.8%), (95.5%±1.4%) and (95.1%±1.3%), respectively, all P>0.05]. Compared with CG group, SL group and SM group, the tissue structure of aGVHD target organs in SH group was severely damaged, with a large number of inflammatory cells infiltratedand higher histopathological scores than SL group and SM group (all P<0.05). Conclusion: For aging CB6F1 mice, after 6 Gy TBI pretreatment with linear accelerator X-ray, PBMC (1×107/each) and spleen cells (2.0×107/each) were injected to successfully induce aGVHD model after non-myelablative haplo-PSCT.

Effects of fermented soymilk with Lacticaseibacillus paracasei YIT 9029 on gut microbiota and defecation habits: a randomised, double-blind, placebo-controlled study.

Previous studies have demonstrated that soymilk and Lacticaseibacillus paracasei YIT 9029 (strain Shirota: LcS) each beneficially affect the gut microbiota and defecation habits. To investigate the effects of daily consumption of fermented soymilk containing LcS (FSM), we conducted a randomised, double-blind, placebo-controlled study of 112 healthy Japanese adults with a low faecal Bifidobacterium count. They consumed 100ml FSM or placebo (unfermented soymilk base) once daily for 4 weeks. Their gut microbiota was analysed by 16S rRNA gene amplicon sequencing and quantitative reverse transcription-polymerase chain reaction (PCR), and faecal short-chain fatty acids (SCFAs) and urinary putrefactive products were assessed during the pre- and post-consumption periods. Defecation habits were examined weekly using a subjective questionnaire. In the post-consumption period, living LcS were not detected in two subjects in the FSM group (n= 57) but were detected in one subject in the SM group (n= 55). The FSM group had a significantly higher number and relative abundance of faecal lactobacilli compared with the placebo group. The relative abundance of Bifidobacterium, alpha-diversity of microbiota, and concentrations of acetate and total SCFAs in faeces were significantly increased in the FSM group, although no significant differences were detected between the groups. The number of defecations and defecation days per week significantly increased in both groups. Subgroup analysis of 109 subjects, excluding 3 with inconsistent LcS detection (2 and 1 subjects in the FSM and SM groups, respectively), revealed that the FSM group (n= 55) had significantly greater increases in faecal acetate concentration compared with the SM group (n= 54) and significant upregulation of pathways related to energy production or glucose metabolism in the gut microbiota. These findings suggest that daily FSM consumption improves the gut microbiota and intestinal environment in healthy adults and may help to maintain health and prevent diseases. Registered at the University Hospital Medical Information Network (UMIN) clinical trials registry under: UMIN 000035612.