Greenhouse Evaluation Research Articles

A Water-Soluble β-Triketone Enriched Extract of Manuka Oil Has Increased Weed Control Efficacy Compared to Vinegar and D-Limonene in a Field and Greenhouse Evaluation

A Water-Soluble β-Triketone Enriched Extract of Manuka Oil Has Increased Weed Control Efficacy Compared to Vinegar and <i>D</i>-Limonene in a Field and Greenhouse Evaluation

An update in Claviceps paspali disease: a comprehensive analysis on field and greenhouse Paspalum spp. Infection.

Paspalum L. is a genus of the Poaceae family, with many species serving as well-adapted forage plants in subtropical climates and continuous grazing systems. However, Claviceps paspali, an ascomycete of the order Hypocreales, represents a major threat to Paspalum species. This fungus induces ergot disease, characterized by the replacement of infected flower seeds with sclerotia, which adversely affects seed production and animal health through mycotoxin production. Although ergot disease is reported in many countries, no totally resistant Paspalum cultivars have been reported for commercial use. This study comparatively evaluated disease development in six Paspalum species under greenhouse conditions with three specific isolates of C. paspali, along with field trials. In addition, field isolates of C. paspali were analyzed for phenotypic characteristics and phylogenetic relationships. Greenhouse evaluation revealed variable susceptibility among Paspalum species, with P. malacophyllum,P. notatum and P. umbrosum being the most resistant. In field trials P. dilatatum showed the highest severity index, while P. umbrosum and P. malacophyllum were less affected. Phenotypic characterization of 22 C. paspali isolates showed variability in pigmentation and growth rates, with PDA being the culture medium where the highest growth rate was observed. Phylogenetic analysis of the isolates identified two well-supported lineages between C. paspali species. This research reports an ergot resistance gradient among Paspalum species, identifying genuine sources of resistance. In addition, virulent isolates of C. paspali potentially useful for rapid screening of accessions or crosses in Paspalum breeding programs were identified.

Impact of crop rotation and tillage operations on mitigating greenhouse gas emissions and evaluation of sustainability index in rice- wheat-green gram cropping system of north Bihar

In North Bihar (NB), the conventional rice-wheat cropping system has led to soil, water, and environmental degradation, alongside low profitability, threatening sustainability. To address these concerns, a thorough field research was conducted over the course of three years to assess different methods of tillage and crop establishment in a rice, wheat, and greengram cycle. The experiment involved five scenarios with different combinations of crop rotation, tillage techniques, seeding procedures, fertilizer use, and irrigation strategies. Uncertainty analysis showed no significant change in mean and variance estimation among seven scenario replications at 5% significance level. Compared to traditional farming (SN-1), managing DSR-rice (SN-5) increased profitability by 17.56%, improved energy use efficiency (EUE) by 32.16%, and reduced irrigation by 24.76% and global warming potential (GWP) by 23.46%. Similarly, substituting zero tillage wheat (ZTW) SN-5 resulted in comparable profitability gains (18.25%) and significant improvements in irrigation (10 %), EUE (+48.65%), and GWP (−20 %) compared to SN-1. Green gram ZT also showed increased profitability (17.35%), with notable improvements in EUE (+38.31%) and GWP (−12.92%) compared to SN-1. Principal component and correlation analyses revealed relationships between total energy inputs, yields, economic returns, and sustainability indices, highlighting the benefits of crop rotation and tillage practices in optimizing resource use. The study suggests that compared to conventional systems, significant improvements in productivity, profitability, energy-use efficiency, and environmental mitigation can be achieved with Crop Rotation and Tillage Operations techniques.

The Identification of the Peanut Wild Relative Arachis stenosperma as a Source of Resistance to Stem Rot and Analyses of Genomic Regions Conferring Disease Resistance through QTL Mapping

Peanut stem rot, also known as white mold, poses a significant threat to peanut production. It is typically managed using fungicides and moderately resistant cultivars. Cultivars with higher resistance can reduce fungicide dependency and increase sustainability. This study explores the potential of wild peanut species in stem rot resistance breeding programs by enhancing genetic diversity in cultivated peanut. Through greenhouse and field evaluations, 13 allotetraploid hybrids with Arachis stenosperma as one of the parents showed superior resistance compared to other wild genotypes. The genomic regions that confer the stem rot resistance were further identified by genotyping and phenotyping an F2 population derived from the allotetraploid ValSten1 (A. valida × A. stenosperma)4× and A. hypogaea cv. TifGP-2. A linkage map was constructed from 1926 SNP markers. QTL analysis revealed both beneficial and deleterious loci, with two resistance-associated QTLs derived from A. stenosperma and four susceptibility loci, two from A. stenosperma and two from A. valida. This is the first study that evaluated peanut-compatible wild-derived allotetraploids for stem rot resistance and that identified wild-derived QTLs for resistance to this pathogen. The allotetraploid hybrid ValSten1, that has A. stenosperma as one of the parents, offers a resource for resistance breeding. Markers associated with resistance QTLs can facilitate introgression from ValSten1 into cultivated peanut varieties in future breeding efforts, potentially reducing reliance on chemical control measures.

Resistance of bread wheat (Triticum aestivum L.) varieties to fusarium head blight (Fusarium graminearum) in Ethiopia

AbstractFusarium head blight (FHB) is a devastating disease reduces wheat yield and quality. This study was aimed to evaluate wheat varieties' responses to spray inoculation with Fusarium graminearum mixture isolates under greenhouse conditions. The treatments were laid out in a randomized completely block design with three replications using 24 bread wheat varieties registered in Ethiopia. The varieties inoculated with mixed inoculum derived from four pathogenic isolates of F. graminearum that had been isolated from wheat kernels. Disease severity was evaluated using a 1–9 scale based on the proportion of bleached spikelets, and the area under disease progress curve (AUDPC) was determined from the disease severity data. At harvest, the kernel weight reduction was determined in comparison to the control. The evaluated traits were significantly interrelated and showed high and significant variation among (p &lt; 0.0001) wheat varieties. Disease severity index among the varieties varied from 29% to 72%, while AUDPC varied from 326%‐ to 1010%‐days. The disease progress rate of the Kingbird variety inoculated with F. graminearum was the slowest (0.0191 units day−1), whereas Ogolcho had the fastest disease progression rate (0.0581 units day−1). Kingbird, Wane, and Limu were moderately resistant, with lower disease severity, AUDPC, and a reduction in 1000‐grain weight and grain weight per spike. Dereselgne, Dambal, and Ogolcho varieties were highly susceptible, with the greatest grain weight per spike reduction (53.2%, 41.4%, and 37.4%) and 1000‐grain weight reduction (41.5%, 42.8%, and 37.5%), respectively. The results implied that there were different levels of FHB resistance in Ethiopian bread wheat varieties. Although current greenhouse evaluation of varieties gives encouraging results, field testing is required to confirm the current findings.

A comparison of procedures for evaluating and selecting alfalfa landrace germplasm for tolerance to salinity

AbstractIn arid and semiarid regions, salinity may affect alfalfa (Medicago sativa) productivity and survival due to either cultivation on salt‐affected soils or the use of salinized irrigation water. Exploiting germplasm evolved under salt‐stress conditions offers opportunities for crop tolerance improvement. In the first phase of the current study, four reportedly salt‐tolerant landraces originated from stress‐prone areas of West Asia or North Africa and two reference commercial cultivars underwent three evaluation trials according to different methods, namely, seed germination in saline water, in vitro testing of young plants, and greenhouse evaluation of adult plants in Cone‐tainers. Experimental populations obtained by intercrossing landrace genotypes selected according to each evaluation method were subsequently evaluated under stressful field conditions in two regions featuring different salt stress type, namely, southern Tunisia (irrigation with saline water) and northwestern Argentina (rainfed cropping in saline soil). Landrace ranking for salt tolerance differed somewhat depending on the evaluation method, and the proportion of selected plants per landrace depended accordingly on the method. In each field experiment, there were two evaluation phases, and the second phase corresponded in both cases to harsher conditions. The in vitro evaluation and selection resulted in potentially more useful selected germplasm than the other evaluation methods. The field experiments highlighted the large specific adaptation effects that affected the response of salt‐tolerant germplasm across the two regions.

Screening of Lablab purpureus (L.) Genotypes against Alternaria Leaf Spot Resistance

Background: Lablab purpureus (L.) bean research in Uttar Pradesh has given less emphasis on improving quality traits preferred by farmers and focused mostly on developing varieties that are high yielding. Though, there are some released Lablab purpureus (L.) varieties farmers mostly prefer to grow their own landrace. Moreover, analysis of farmers’ perceptions and views on Lablab purpureus (L.) bean diseases and control methods utilized by farmers has been minimal. Farmers’ insights and understanding of crop diseases play an important role in sustainable disease management. Therefore, there is a need to assess farmers’ priority problems for Lablab purpureus (L.) bean production, their variety preference and their perception of the leaf spot disease. Methods: In our study, the landraces were assessed both in the field and in the greenhouse during 2021-22 with Alternaria doliconidium and Alternaria destruens isolates. Result: The highest level of resistance were found Under greenhouse evaluation in VRSEM-757, VRSEM-776, VRSEM-730 and VRSEM-739 to Alternaria doliconidium whereas genotypes found to be resistance under field evaluation were VRSEM-799, VRSEM-757 and VRSEM-776. Similarly, genotypes found to be resistance to Alternaria destruens were VRSEM- 757 and VRSEM- 702 under greenhouse evaluation whereas, genotypes VRSEM-776 found to be resistance during field evaluation.However, the resistance were moderate. Overall, resistance were highly heritable, suggesting that phenotypic selection can be exploited to improve leaf spot resistance in lablab bean varieties.

Fhb9, a major QTL for Fusarium head blight resistance improvement in wheat

Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is one of the most devastating diseases of wheat worldwide. Identification and validation of major quantitative trait loci (QTLs) for FHB resistance without negative effects on agronomic traits is critical to success in breeding FHB-resistant cultivars. In this study, a stable major QTL on chromosome arm 2DL was identified by evaluating a recombinant inbred line (RIL) population derived from Shi4185×Shijiazhuang 8 in both field and greenhouse experiments. QTL mapping and pedigree analyses indicated that the 2DL QTL is the same QTL as QFhb-2DL previously identified in Ji5265, therefore, designated Fhb9. Four kompetitive amplicon sequence polymorphism (KASP) markers were developed based on exome capture sequencing data to enhance marker density in the Fhb9 region, and it was delimited to an interval between single nucleotide polymorphism (SNP) markers KASP-12056 (533.8) and KASP-525 (525.9 Mb) explained 26.0-30.1% of the phenotypic variation. Analysis of the geographic distribution of the Fhb9 resistance allele suggested that it originated from Huang-Huai winter wheat region in China, and very low frequency of Fhb9 in modern Chinese cultivars reveals that it has not been widely deployed in breeding programs. Field and greenhouse evaluation of yield-related traits of near-isogenic lines (NILs) contrasting in Fhb9 alleles indicated that Fhb9 resistance allele did not show any adverse effects on those traits. Fhb9 showed an additive effect on enhancing FHB resistance with Fhb1. Therefore, Fhb9 is a valuable major QTL for improving FHB resistance in wheat and the near-diagnostic markers developed in this study will facilitate its deployment in wheat breeding programs.

Design and Performance Evaluation of a Photovoltaic Greenhouse as an Energy Hub with Battery Storage and an Electric Vehicle Charger

This work presents a photovoltaic greenhouse’s design and performance evaluation as an energy hub in modern agriculture that integrates battery energy storage, an electric vehicle charging station, and non-controlled loads. The greenhouse roof comprises 48 semi-transparent photovoltaic panels with nominal transparency of 20% and 110 W capacity. The control of the photovoltaic greenhouse as an energy hub was approached as an optimization problem with the aim of minimizing the energy purchased from the grid. The simulation results indicate that the system is capable of balancing power transactions within the microgrid, thus enabling electromobility and, at the same time, achieving an average energy saving of up to 41%. Furthermore, it was found that the case of slow charging of the electric vehicle at night was less demanding on the battery system than fast charging during the day in terms of abrupt power transitions and average state of charge of the battery system, 61% vs. 53%, respectively. Empirical results also demonstrated the negative impact of soiling generated by agricultural activity on the performance of solar panels. For a period analyzed of three years, an average annual production loss of 6.8% was calculated.

Efficacy of Conventional and Biorational Insecticides against the Invasive Pest Thrips parvispinus (Thysanoptera: Thripidae) under Containment Conditions.

In 2020, the invasive Thrips parvispinus (Karny) was first detected in Florida, United States. In response to the implemented regulatory restrictions, we conducted laboratory experiments under containment conditions. Thrips larvae and adults were exposed to 32 products (conventional and biorational insecticides) either directly or indirectly. Direct exposure was performed using a Spray Potter Tower, while indirect exposure was conducted by evaluating residue toxicity against the thrips. Water served as a control. We assessed mortality and leaf-feeding damage 48 h post-treatment. Among the conventional insecticides, chlorfenapyr, sulfoxaflor-spinetoram, and spinosad caused high mortality across all stages in both direct and residue toxicity assays. Pyridalyl, acetamiprid, tolfenpyrad, cyclaniliprole-flonicamid, acephate, novaluron, abamectin, cyantraniliprole, imidacloprid, cyclaniliprole, spirotetramat, and carbaryl displayed moderate toxicity, affecting at least two stages in either exposure route. Additionally, chlorfenapyr, spinosad, sulfoxaflor-spinetoram, pyridalyl, acetamiprid, cyclaniliprole, cyclaniliprole-flonicamid, abamectin, and acephate inhibited larvae and adult's leaf-feeding damage in both direct and residue toxicity assays. Regarding biorational insecticides, mineral oil (3%) and sesame oil caused the highest mortality and lowest leaf-feeding damage. Greenhouse evaluations of spinosad, chlorfenapyr, sulfoxaflor-spinetoram, and pyridalyl are recommended. Also, a rotation program incorporating these products, while considering different modes of action, is advised for ornamental growers to avoid resistance and to comply with regulations.

Greenhouse Evaluation of Biochar-Based Controlled-Release Nitrogen Fertilizer in Corn Production

Greenhouse Evaluation of Biochar-Based Controlled-Release Nitrogen Fertilizer in Corn Production

Development of a Greenhouse Method to Evaluate the Peanut Resistance to Athelia rolfsii

Peanut stem rot (also known as white mold), caused by the fungus Athelia rolfsii, is one of the most damaging soilborne pathogens for U.S. peanut production. The disease is mainly controlled by fungicides and by adopting cultivars with moderate resistance. Field evaluation is the main approach for evaluating plant resistance, but it is costly and labor-intensive. Reliable methods for in vitro or greenhouse evaluations are desirable. Greenhouse, growth chamber, and in vitro methods have been tried to assess resistance, but they generally do not correlate well with field results. In this study, we developed a reliable method to assess resistance to stem rot on peanut under greenhouse conditions. Stem cuttings were taken from 60-day-old plants and placed into a cup filled with potting mix. Cuttings were inoculated with active A. rolfsii mycelial plugs above the soil line, with mycelium directly contacting the stem. Inoculated cuttings were put in a mist chamber to keep humidity high. Highly significant differences in disease resistance among genotypes were found at 7 days after inoculation ( P &lt; 0.05). Moreover, evaluations done at 3, 5, 7, and 9 days after inoculation and the area under the disease progress curve were significantly correlated with field evaluations of the same genotypes. This greenhouse assay is a step forward to screen peanut germplasm more efficiently for stem rot resistance. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

Effect of phyllosphere and spike mycoflora on castor gray mold incited by Amphobotrys ricini

Gray mold of castor incited by Amphobotrys ricini is an economically important disease causing heavy lossesduring capsule formation stage under high humid conditions. The phyllosphere and spike mycoflora were isolated from castor and tested for their bioefficacy against gray mold under laboratory and greenhouse conditions. The mycoflora isolated were identified based on morphological characters as species of Acremonium, Aspergillus, Choanephora, Cladosporium, Fusarium, Macrophomina and Penicillium. Among the mycoflora tested in vitro, highest inhibition of the test pathogen was recorded with Acremonium species. Greenhouse evaluation of phyllosphere and spike mycoflora by detached spike method has shown that inoculation of detached spikes with Penicillium spp. 24 hours prior to pathogen inoculation has significantly reduced gray mold severity compared to control.

Assessment of Phytoextracts and Synthetic Chemicals for Controlling Leaf Blight of Syzygium Cumini

Jamun (Syzygium cumini) is a distinguished source of protein, fat, minerals (Iron, Calcium, and Potassium), fiber, carbohydrates, phosphorous, and vitamin C (ascorbic acid). Leaf blight of S. cumini, caused by Pestalotiopsis spp., poses a potential threat to the successful production of Jamun fruit. The appearance of small, round, dark, and sunken spots on the fruit diminishes its quality and results in yield losses of 10 to 20%. Fungicides and plant extracts were employed to address this fungal problem under laboratory and greenhouse conditions using a Completely Randomized Design whereas field trials were performed using a Randomized Complete Block Design. In vitro experiments revealed that among the fungicides, minimum fungal growth was observed with Score (12.5 mm), followed by Topsin M (16.89 mm), Fossil (18.37 mm), Excel (23.17 mm), Evito (27.56 mm), and Bloom (32.32 mm), as compared to the control (55.56 mm). Among the phytoextracts, Moringa extracts showed the least fungal growth (15.7 mm), followed by Neem (18.76 mm), Eucalyptus (19 mm), Garlic (22.72 mm), Ginger (27.57 mm), and Cinnamomum verum (Dar Cheni) (31 mm), in comparison to the control (53.17 mm). The most effective fungicides and plant extracts determined in the laboratory experiments were further evaluated in greenhouse and field conditions, both alone and in combinations. In the greenhouse evaluation, the combination of Moringa + Score exhibited the lowest disease incidence (23.63%), followed by Score (28.12%) and Moringa (29.56%), in contrast to the control (52.9%). Under field conditions, among all treatments, Moringa + Score exhibited the least disease incidence of 17.44%. These findings confirm that leaf blight of S. cumini can be managed by using Score fungicide and Moringa oleifera extract.

Mutations in the acetolactate synthase (ALS) enzyme affect shattercane (Sorghum bicolor) response to ALS-inhibiting herbicides

BackgroundShattercane [Sorghum bicolor (L.) Moench ssp. Arundinaceum (Desv.)] is a competitive weed in North America's corn, soybean, sorghum, and other agronomic crops. Control of shattercane with POST herbicides in corn became possible with the introduction of acetolactate synthase (ALS)-inhibiting herbicides in the 1980s, and their extensive use resulted in the evolution of ALS-inhibitors resistant shattercane.ResultsShattercane seeds were collected from 16 south-eastern and south-central Nebraska fields that were treated with primisulfuron for three consecutive years. Three resistant plants were found in greenhouse evaluations of more than 30,000 plants. Results from a greenhouse bioassay conducted to assess the response of each shattercane biotype to ALS-inhibiting herbicides showed a differential response to ALS inhibitors within and between chemical classes. Biotype P8-30 was resistant or partially resistant to all ALS-inhibiting herbicides applied and displayed a unique amino acid sequence substitution (Trp574 to Leu) relative to the other two resistant biotypes, P2-205 and P9-102. Whole plant dose–response studies confirmed a 4- to the 12-fold level of primisulfuron resistance in three shattercane biotypes compared with the known primisulfuron-susceptible shattercane biotype. The ALS gene was sequenced using primers designed from the corn ALS sequence to identify mutations in the ALS gene that confer resistance. A total of seven nucleotide substitutions were detected in the three herbicide-resistant biotypes P2-205, P8-30, and P9-102. These biotypes are being crossed to adapted sorghum lines (grain, sweet, and forage) to broaden germplasm with resistance to ALS-inhibiting herbicides.ConclusionThe discovery of these mutants should accelerate the development of sorghum genotypes that tolerate ALS-based herbicides, which provide additional choices for sorghum farmers to control weeds, especially grasses, in their fields.

Design and thermal performance of an innovative greenhouse

The study presents the design and thermal performance evaluation of a novel solar greenhouse with humidification-dehumidification unit, water-cooled heat exchanger and variable mixing ratio in the Mediterranean climate. The greenhouse is designed to provide proper microclimatic conditions for crops, produce fresh water through condensing water vapor released from the plants, and save energy using the semitransparent PV panels. Results showed that on a typical summer day, the solar greenhouse ensures proper microclimatic conditions all day long by reducing the temperature by 11.14 °C compared to conventional greenhouses, while maintaining acceptable values of relative humidity and producing 70 L of fresh water per day. An operation schedule was established based on varying return-to-fresh air ratio to ensure optimal system performance. The operation schedule showed that proper microclimatic conditions can be reached through ventilation for 13 h per day. To enhance the net-energy performance, the produced water can be used for irrigation and the return air can be used for cooling the PV panels.

Energy performance assessment of photovoltaic greenhouses in summer based on coupled optical-electrical-thermal models and plant growth requirements

Photovoltaic (PV) greenhouses are widely used to regulate internal irradiance and air temperature to create optimal climatic conditions for plant growth. Therefore, comprehensive multidomain energy models of PV greenhouses are more likely needed to improve the system performance. However, few studies investigate optical-electrical-thermal characteristics and PV greenhouse evaluation indicators based on crop growth requirements. The aim of this work is to develop coupled optical-electrical-thermal models to assess the energy performance of the PV greenhouse with different PV roof coverage ratios during the summer months. In addition, the models of photosynthetically active radiation (PAR) and net photosynthetic rate (PN) are presented based on the irradiance and temperature of the PV greenhouse to evaluate the dynamic response of crops to interior climate conditions. The models are validated with experiments on the PV greenhouse in Kunming, with a total area of 26.25 m2. The electricity supply and demand systems include PV modules (3.285 kWp), a battery bank (24 kWh), an air–water heat pump (AWHP), and spray combined with fan cooling. Two cooling scenarios are analysed and compared by varying the PV module coverage ratio. Scenario 1 (S.1) is AWHP cooling, and scenario 2 (S.2) is spray combined with fan cooling. The optimum interior temperature and PAR of the PV greenhouse are 22 °C and 300 μmol·m−2·s−1, respectively. The simulations indicate that if the proportion of the PV layout on the greenhouse roof is greater than 20%, the year-round PAR inside the greenhouse will be below the threshold value of 550 μmol·m−2·s−1 at which the strawberry PN is saturated. For every 10% increase in PV roof coverage, the interior air temperature decreases by 0.02–0.56 °C corresponding to a daily cooling load reduction of 0.45–1.02 kWh/d, while the PV generation increases by 1.7–3.19 kWh/d and the maximum electricity consumption of S.1 and S.2 drops by 0.66 kWh/d and 0.52 kWh/d, respectively. Moreover, the high self-consumption-sufficiency balance (SCSB) values show that the PV and battery sizes are 2.22–2.78 kWp (40–50% coverage) and 15.72–15.75 kWh for S.1, and those in S.2 are 1.11–1.67 kWp (10–30% coverage) and 0.66–3.22 kWh. The coefficient of performance (COP) of the system reaches a maximum of 0.45–1.12 at 40% coverage in S.1 and 1.88–3.01 at 10–30% coverage in S.2. When the PV module coverage is 40% in S.1, the levelized costs of electricity (LCOE) and levelized costs of cooling (LCOC) are the lowest at 0.0760USD/kWhel and 0.0693USD/kWhc. Similarly, the lowest LCOE and LCOC of S.2 are 0.0306USD/kWhel and 0.0210USD/kWhc, as the PV module coverage is 30%. The study will be beneficial for applying photovoltaic modules, battery banks, and cooling technologies in the greenhouse, especially considering the dynamic crop response to internal climates and the energy performance assessment.

Surveillance of Bacterial Canker of Peach in Azad Jammu and Kashmir, and its Bio-Management

Bacterial canker of peach caused by Pseudomonas syringae is an increasing problem in Azad Jammu Kashmir with more than 50 pathovars on the basis of host. A detailed survey was conducted in peach growing areas of Azad Jammu and Kashmir for the determination of disease prevalence and incidence. Pathogen was isolated and characterized from the collected samples using LOPAT and GaTTA scheme. Furthermore the results of biochemical identification was confirmed using PCR followed by sequencing of obtained expected bands. For the bio-management of virulent pathogenic strains indigenous rhizobacterial isolates were obtained, characterized and evaluated using dual culture as well as in a greenhouse pot experiment against P. syringae pathovars. During a positive sampling method used for the surveillance it was found that maximum disease prevalence was 75% in district Bagh while there was 25% disease prevalence found in Haveli and Sudhanoti. Similarly maximum disease incidence was found in district Bagh that was 16%. A total of 32 isolates were recovered from the collected samples that were further screened on the basis of their virulence. It was found that the total of 18 isolates screened were similar to the already reported strains of P. syringae pv. syringae (Pss) on the basis of biochemical and molecular tools. From total 17 rhizobacterial isolates recovered were subjected to dual culture technique and was found that 06 isolates showed promising zone of inhibition upto 11.5 mm. The results of greenhouse evaluation revealed that the minimum disease incidence was recorded 3% using R-9 and R-17 in consortium against Pss as compared to the control treatment (39%). It was concluded that the ecofriendly management practices against this destructive disease must be adopted and appreciated

Field and Greenhouse Assessment of Seed Treatment Fungicides for Management of Sudden Death Syndrome and Yield Response of Soybean.

Seed treatments for the management of sudden death syndrome (SDS) caused by Fusarium virguliforme are available in the United States and Canada; however, side-by-side comparisons of these seed treatments are lacking. Sixteen field experiments were established in Illinois, Indiana, Iowa, Michigan, and Wisconsin, United States, and Ontario, Canada, in 2019 and 2020 to evaluate seed treatment combinations. Treatments included a nontreated check (NTC), fungicide and insecticide base seed treatments (base), fluopyram, base + fluopyram, base + saponin extracts from Chenopodium quinoa, base + fluopyram + heat-killed Burkholderia rinojenses, base + pydiflumetofen, base + thiabendazole + heat-killed B. rinojenses, and base + thiabendazole + C. quinoa extracts + heat-killed B. rinojenses. Treatments were tested on SDS moderately resistant and susceptible soybean cultivars at each location. Overall, NTC and base had the most root rot, most foliar disease index (FDX), and lowest yield. Base + fluopyram and base + pydiflumetofen were most effective for managing SDS. Moderately resistant cultivars reduced FDX in both years but visual root rot was greater on the moderately resistant than the susceptible cultivars in 2020. Yield response to cultivar was also inconsistent between the 2 years. In 2020, the susceptible cultivar provided significantly more yield than the moderately resistant cultivar. Treatment effect for root rot and FDX was similar in field and greenhouse evaluations. These results reinforce the need to include root rot evaluations in addition to foliar disease evaluations in the breeding process for resistance to F. virguliforme and highlights the importance of an integrated SDS management plan because not a single management tactic alone provides adequate control of the disease.

Evaluation of Greenhouse and Field Performances of Depar F1 Tomato Varıety Grafted on Dıfferent Rootstocks

In this study, the effects of tomato (Solanum Lycopersicum cv. Depar F1) seedlings grafted on three different rootstocks (Kudret, Hamarat, Pençe) on yield and some quality parameters of tomato plants grown in the greenhouse and open field were investigated. According to the results we obtained, while the plants grown in greenhouses grew up 42.3% faster than those grown in the open, an increase in yield was achieved by 265%. While TA and vitamin C values, among the fruit quality characteristics, came to the fore in the plants grown in the greenhouse, the fruit firmness values came to the fore in the tomatoes grown in the field. Kudreç rootstock came to the fore regarding yield and fruit quality characteristics. When the nutrient contents of the fruits were examined, the highest N, Ca and Mg were measured in greenhouse-grown tomatoes, while the highest O.M and Mn were measured in field plants. Hamarat rootstock has come to the fore in terms of fruit nutritional content. As a result, the importance of growing conditions for grafted plants to show their performance has been demonstrated again. However, it has been demonstrated for the first time that such successful results can be obtained, especially with organic plant nutrition methods.