Green Synthesized Iron Oxide Nanoparticles Research Articles

Evaluating the Photocatalytic Activity of Green Synthesized Iron Oxide Nanoparticles

Water pollution from dyes is a major environmental challenge, demanding advanced materials for efficient degradation. In this study, we synthesized iron oxide nanoparticles (IONPs) using an aqueous extract of Senegalia catechu leaves and evaluated their photocatalytic activity in methylene blue (MB) dye degradation under sunlight irradiation. The IONPs were characterized by UV-visible spectroscopy (UV–vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray Spectroscopy (EDS). XRD pattern showed a highly crystalline structure with an average crystallite size of 34.7 nm, while SEM images revealed predominantly spherical particles with uneven surface texture. Photocatalytic efficiency exceeded 80% MB dye degradation after 120 min of sunlight exposure. Optimization of catalyst dose, pH, dye concentration, and other parameters is essential for maximizing degradation efficiency. The IONPs demonstrated reusability over four degradation cycles, retaining effective photocatalytic performance. This study underscores the potential of green-synthesized IONPs as eco-friendly photocatalysts for wastewater treatment and environmental remediation.

Green synthesized iron oxide nanoparticles as a potential regulator of callus growth, plant physiology, antioxidative and microbial contamination in Oryza sativa L.

In tissue culture, efficient nutrient availability and effective control of callus contamination are crucial for successful plantlet regeneration. This study was aimed to enhance callogenesis, callus regeneration, control callus contamination, and substitute iron (Fe) source with FeO-NPs in Murashige and Skoog (MS) media. Nanogreen iron oxide (FeO-NPs) were synthesized and well characterized with sizes ranging from 2 to 7.5 nm. FeO-NPs as a supplement in MS media at 15 ppm, significantly controlled callus contamination by (80%). Results indicated that FeCl3-based FeO-NPs induced fast callus induction (72%) and regeneration (43%), in contrast FeSO4-based FeO-NPs resulted in increased callus weight (516%), diameter (300%), number of shoots (200%), and roots (114%). Modified media with FeO-NPs as the Fe source induced fast callogenesis and regeneration compared to normal MS media. FeO-NPs, when applied foliar spray, increased Plant fresh biomass by 133% and spike weight by 350%. Plant height increased by 54% and 33%, the number of spikes by 50% and 265%, and Chlorophyll content by 51% and 34% in IRRI-6 and Kissan Basmati, respectively. Additionally, APX (Ascorbate peroxidase), SOD (Superoxide dismutase), POD (peroxidase), and CAT (catalase) increased in IRRI-6 by 27%, 29%, 283%, 62%, while in Kissan Basmati, APX increased by 70%, SOD decreased by 28%, and POD and CAT increased by 89% and 98%, respectively. Finally, FeO-NPs effectively substituted Fe source in MS media, shorten the plant life cycle, and increase chlorophyll content as well as APX, SOD, POD, and CAT activities. This protocol is applicable for tissue culture in other cereal crops as well.

Interplay between green-synthesized nanoparticles and plant performance is mediated by the microbial community in the rhizocompartments

Numerous green-synthesized nanoparticles are being evaluated for their potential application in soil, yet understanding their influence on plant performance and their rhizocompartments associated microbes is a gap that needs to be addressed. In this study, we investigated how applying iron oxide nanoparticles (control 0, low 25 and high 50 mg/kg) impacted the rhizocompartments associated microbial communities and plant secondary metabolites grown in natural soil microcosm. The transcriptional analysis showed that the majority of artemisinin and flavonoid biosynthetic pathway genes were significantly upregulated in plants with the application of low concentration of green-synthesized iron oxide nanoparticles (FeO.NPs) when compared with control plants, suggesting a fine collaboration between these metabolites’ pathways, as indicated by a remarkable increase in artemisinin content (2-fold), ferulic acid (7.6-fold), luteolin (4.3-fold) and syringic acid (6.2-fold). The response of rhizocompartments microbial communities and associated soil enzymes was investigated using high-throughput sequencing and profiler test kits, respectively. The soil microbial community structure was altered upon FeO.NPs exposure in both low and high treated soils as indicated by multivariate analysis. For instance, bacterial phyla Bacteroidetes and Gemmatimonadetes and fungal phyla Ascomycota demonstrated a significant rise in relative abundance in both the rhizosphere and rhizoplane in low and high treatments relative to the control. Moreover, genera level analysis revealed that Cupriavidus, Vibrionimonas, and Burkholderia displayed higher relative abundance (p < 0.05) in low FeO.NPs concentration while Giaella and Nonomuraea didn't show any noticeable response to FeO.NPs. In terms of fungal genera, Oidiodendron and Cryptococcus showed high relative abundance under low treatment. The altered microbiome profile is possibly associated with soil enzymes, for instance, urease and catalase activities were increased by 1.76 and 1.71-fold, respectively in low FeO.NPs. Thus, this study demonstrates the effects of FeO.NPs gradients on plant secondary metabolites and soil enzyme activities, while contributing new insights into their specific impacts on the microbial community composition in the rhizocompartments of plant root system.

Assessment of the neuroprotective effect of green synthesized iron oxide nanoparticles capped with curcumin against a rat model of Parkinson's disease.

The current study aims to investigate the protective effect of iron oxide nanoparticles capped with curcumin (FeONPs-Cur) against motor impairment and neurochemical changes in a rat model of Parkinson's disease (PD) induced by reserpine. Rats were grouped into control, PD model induced by reserpine, and PD model treated with FeONPs-Cur (8 rats/group). The open field test was used to assess motor activity. The concentration of dopamine (DA), norepinephrine (NE), serotonin (5-HT), lipid peroxidation (MDA), reduced glutathione (GSH), and nitric oxide (NO), and the activities of Na+,K+,ATPase, acetylcholinesterase (AchE), and monoamine oxidase (MAO) were determined in the midbrain and striatum. Data were analyzed by ANOVA at P-value<0.05. The PD model exhibited a decrease in motor activity. In the midbrain and striatum of the PD model, DA, NE, and 5-HT levels decreased significantly (P-value<0.05). However, an increase in MAO, NO, and MDA was observed. GSH, AchE and Na+,K+,ATPase decreased significantly in the two brain areas. FeONPs-Cur prevented the decline of dopamine and norepinephrine and reduced oxidative stress in both areas. It also prevented the increased MAO activity in the two areas and the inhibited activity of AchE and Na+,K+,ATPase in the midbrain. These changes were associated with improvements in motor activity. The present data indicate that FeONPs-Cur could prevent the motor deficits induced in the PD rat model by restoring dopamine and norepinephrine in the midbrain and striatum. The antioxidant activity of FeONPs-Cur contributed to its protective effect. These effects nominate FeONPs-Cur as an antiparkinsonian candidate.

Review—Potential of Tunneling Magnetoresistance Coupled to Iron Oxide Nanoparticles as a Novel Transducer for Biosensors-on-Chip

Biosensors-on-chip (BoC), compact and affordable public diagnostic devices, are vital for preventing health crises caused by viral and bacterial mutations, climate change, and poor diets. Clinical, remote, and field use are possible with these devices. BoC is used in food safety, environmental monitoring, and medical diagnosis. The coupling of tunneling magnetoresistance (TMR) sensing elements in chip form with surface functionalized iron oxide nanoparticles (IONPs) as a biomarker, known as TMR/ IONPs, allows BoC devices to be made. The functional framework of BoC based on TMR/ IONPs, the instrument system, and biomolecule immobilization will be covered in this review. This review aims to overview the recent research on a biosensor using TMR technology with IONPs biomarkers and discuss its future advances in point-of-care diagnostics. TMR sensors have revolutionized low-magnetic field sensing technologies, yet biosensing faces challenges. However, it’s important to recognize that TMR sensors exclusively utilize out-of-plane connections. that creates a larger separation between biomolecules and the sensing layer, leading to a decrease in the sensitivity of biomolecules. Large magnetization of dispersed IONPs to generate sufficient stray-field, compact and inexpensive instrumentation to sense the low voltage yielded by the TMR/IONPs system, and high-selectivity bio-analyte immobilization to the surface of IONPs to increase sensor sensitivity are the notable issues to address this problem. The utilization of the green synthesized method in producing IONPs for magnetic labeling can enhance the sensitivity of a TMR-based biosensor. This is achieved through the formation of IONPs with a capping agent on its surface that inhibits the aggregation and facilitates the immobilization of the biomolecule target. Additionally, improving device performance requires creating high-TMR materials. Despite challenges, research and technological advances hold great promise for TMR/GS-IONP bio-applications.

Remediation of Lead-Contaminated Water Using Green Synthesized Iron-Oxide Nanoparticles: Performance and Mechanism

Lead (Pb(II)) ions in water pose a significant threat to both human health and aquatic ecosystems. Various approaches have been employed for wastewater treatment, but adsorption is often preferred due to its effectiveness. However, its practical application is limited by the large quantities of adsorbent required, which consequently increases operational costs. In this study, orange-modified iron-oxide nanoparticles (O-Fe3O4) are synthesized from agro-waste mass (orange peel), and adsorption experiments were conducted for the removal of Pb(II) from aqueous solution. Characterization studies confirm that O-Fe3O4 nanoparticles possess a mesoporous hexagonal nanocrystalline structure, with diameters measuring less than 100 nm. The adsorption process was optimized using a central composite design framework combined with response surface methodology. The analysis of interaction effects demonstrated that they significantly influenced the effectiveness of adsorption removal. The study revealed that an initial concentration of 25 mg/L, a dosage of 0.2 g/L, a contact period of 90 min, and a pH of 5.5 were the optimum conditions to achieve above 95% of Pb(II) removal. The green synthesized O-Fe3O4 nanoparticles, which presented high efficacy, makes it a promising option for implementing the sustainable water purification.

Green and sustainable synthesis of iron oxide nanoparticles for synergetic removal of melanoidin from ethanol distillery simulated model wastewater

This study investigates a novel approach for removal of melanoidin using Fenton oxidation process. Melanoidins are the biproducts of Maillard’s reaction involving polysaccharides and amino acids. Wastewater generated by ethanol distilleries utilize molasses, a common ingredient containing melanoidin, which is also toxic and imparts a dark color. Various wastewater treatments techniques are present, among which advanced oxidation processes (AOPs), particularly Fenton oxidation, have proven effective in eliminating melanoidins from wastewater. The novelty in this study is that green synthesized iron oxide nanoparticles (FeO NPs) are used in replacement of ferric salts to enhance the Fenton oxidation process for efficient melanoidin removal. The green synthesis provides stability to FeO NPs. FeO NPs were extensively characterized using techniques like FT-IR, XRD, SEM, and EDX analyses. The analyses confirmed that the FeO NPs exhibited a crystalline structure, with an average size of 21.5 nm. The crucial element of this study is to deploy the FeO NPs to power the Fenton oxidation reaction for melanoidin removal.

Ameliorative role of green synthesized iron oxide nanoparticles using Punica granatum extract against lead acetate hepato-renal toxicity in albino rat

Ameliorative role of green synthesized iron oxide nanoparticles using Punica granatum extract against lead acetate hepato-renal toxicity in albino rat

Larvicidal activity of green synthesized iron oxide nanoparticles using Grevillea robusta Cunn. leaf extract against vector mosquitoes and their characterization

Extensive fumigation of synthetic pesticides to control the mosquito vector during each post-monsoon season in Pakistan significantly enhanced the environmental contamination and extinction of beneficial insects from the urban ecosystems. In this context, the present study examined the larvicidal efficacy of green synthesized iron nanoparticles (IONPs), using an aqueous leaf extract of Grevillea robusta against the early 2nd and 4th instar larvae of Aedes aegypti and Anopheles stephensi in Pakistan. The prepared IONPs were characterized by UV–Vis spectrum, FTIR, X-ray diffraction, scanning electron microscopy, and energy-dispersive diffraction. Larvicidal bioassay was conducted at various concentrations (80, 160, 240, 320, and 400 ppm) of IONPs prepared from leaf extract of G. robusta, and readings were taken-every 12 h for two consecutive days. In vitro, larvicidal assay, G. robusta leaf extract IONPs exhibited high mortalities of 64–96% (LC50 = 259.07 ppm; LC90 = 443.92 ppm) for the second instar and 65–98% (LC50 = 238.05 ppm; LC90 = 433.93 ppm) for the fourth instar of Ae. aegypti, while in the case of An. stephensi 56–84% (LC50 = 297.96 ppm; LC90 = 528.69 ppm) for the second and 56–88% (LC50 = 292.72 ppm; LC90 = 514.00 ppm) mortality for fourth larvae at 12–48 h post-exposure times were observed respectively. Significant (p < 0.05) dose-dependent and exposure time-dependent trends were observed among the 2nd and 4th larvalinstar of An. stephensi and Ae. aegypti. However, both species showed similar response and observed no significant (p > 0.05) difference in percentage mortality between the vector mosquitoes An. stephensi and Ae. aegypti. Overall, this study demonstrates that the larvicidal efficacy of green synthesized IONPs at low concentrations can be an ideal eco-friendly and cost-effective biocontrol of vector mosquitoes' larvae of An. stephensi and Ae. aegypti.

The Wound Healing effect of the Green Synthesized Iron Oxide Nanoparticles: A Study on Mice

The extract of fig fruit has shown significant medical usefulness in various fields. The entrance of nanotechnology into the field of medicinal and pharmacology has shown remarkable advantages. Plants contain diverse molecules thatcan reduce metals, and provide a safe, eco-friendly approach for synthesizing nanoparticles. Iron oxide nanoparticles (IONPs) have been reported to possess an antimicrobial effect against some strains of bacteria and moulds. We have aimed to synthesize IONPs from fig fruit extract and investigate the influence of fig extract and IONPs in wound healing of mice. UV-Vis spectroscopy, X-ray diffraction (XRD), and field emission scanning electron microscopy were used to characterize the IONPs that were produced. The UV-Vis spectrum has indicated a maximum absorbance of around 400 nm, which is characterized by IONPs. The XRD examination has indicated the formation of α-Fe2O3 NPs, while the morphological examination by FESEM indicated the presence of aggregated rough IONPs. Then, 15 mice were divided into three groups equally; control, fig extract treated, and IONPs treated mice. Mice treated with fig extract or IONPs have shown obvious improvement after 5 days compared to control. On the 14th day, almost complete healing was observed in mice treated with fig fruit extract or IONPs. In conclusion, the possibility of using plants and NPs as antibacterial and anti-inflammatory agents would provide a great advancement in the administration of mild skin injuries.

Effect of green synthesized iron oxide nanoparticles on bacterial microbiome for clean up the crude oil

Effect of green synthesized iron oxide nanoparticles on bacterial microbiome for clean up the crude oil

Multifunctional Iron Oxide Nanocarriers Synthesis for Drug Delivery, Diagnostic Imaging, and Biodistribution Study.

The aim of the current study is to design the radiolabeled and drug-loaded nanocarrier with high loading capacity and pH-dependent drug release characteristics that could effectively transport loaded compounds to various organs for efficient diagnostic imaging and chemotherapeutic drug delivery. The aqueous extract of green tea leaves was used to synthesize the small-sized iron oxide nanoparticles (IONPs). The nanoparticles were characterized with UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray analysis (EDX). Iron oxide nanoparticles with sizes smaller than 50nm were successfully synthesized, making them suitable for in vivo studies. In drug loading trials, 94% of the drug was loaded onto the active surface of iron oxide nanoparticles from the solution. The in vitro drug release study revealed that an acidic environment (pH 4.5) effectively triggers the release of doxorubicin (DOX) from the nanoparticles as compared to a neutral environment (pH 7.4). The gamma-emitting radionuclide 99mTc was successfully labeled with IONPs for biodistribution and imaging studies. The efficiency of radiolabeling was observed to be ≥ 99%. Furthermore, the in vivo biodistribution study of radiolabeled IONPs in rabbit model showed rapid accumulation in various organs such as heart, liver, and kidneys. This work suggested that green synthesized iron oxide nanoparticles are potential nanocarriers for diagnostic imaging and efficiently distributing DOX to specific organs. The aqueous extract of green tea leaves was used for the facile green synthesis of iron oxide nanoparticles (IONPs). Furthermore, the chemotherapeutic drug doxorubicin (DOX) and gamma-emitting radionuclide 99mTc were loaded on these iron oxide nanoparticles to evaluate the in vivo biodistribution and drug delivery studies in the rabbit models.

Incubating Green Synthesized Iron Oxide Nanorods for Proteomics-Derived Motif Exploration: A Fusion to Deep Learning Oncogenesis.

The nanotechnological arena has revolutionized the diagnostic efficacies by investigating the protein corona. This displays provoking proficiencies in determining biomarkers and diagnostic fingerprints for early detection and advanced therapeutics. The green synthesized iron oxide nanoparticles were prepared via Withania coagulans and were well characterized using UV-visible spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and nano-LC mass spectrophotometry. Iron oxides were rod-shaped with an average size of 17.32 nm and have crystalline properties. The as-synthesized nanotool mediated firm nano biointeraction with the proteins in treatment with nine different cancers. The resultant of the proteome series was filtered oddly that highlighted the variant proteins within the differentially expressed proteins on behalf of nano-bioinformatics. Further magnification focused on S13_N, RS15, RAB, and 14_3_3 domains and few abundant motifs that aid scanning biomarkers. The entire set of variant proteins contracting to common proteins elucidates the underlining mechanical proteins that are marginally assessed using the robotic nanotechnology. Additionally, the iron rods indirectly possess a prognostic effect in manipulating expression of proteins through a smarter route. Thereby, such biologically designed nanotools provide a dual approach for medical studies.

Fabrication of Disposable Electrodes Based on Green Synthesized Iron Oxide Nanoparticles for Enumeration of Bacteria in Water

Poor drinking water quality is a leading cause of preventable deaths in young children. Identifying contaminated water is a major challenge, especially where water resources are very limited. There is an urgent need to fabricate smart disposable electrodes to detect specific Escherichia coli ATCC-25922 in water to protect against diseases such as gastroenteritis and acute diarrhea. In this article, we presented a simple method to fabricate disposable electrodes using single-pot green iron oxide nanoparticles. The nanostructure and detailed morphology of the green synthesized iron oxide nanoparticles (Ne-Ne-Fe3O4-NPs) were analyzed using analytical techniques. Modified Ne-Fe3O4-NPs developed the disposable electrode and cyclic voltammetry (CV) was used to detect the target bacteria. Under optimized experimental conditions, the signals increased linearly while the concentration range increased from 101−107 CFU ml−1, having a short response of time 35 min. In addition, the fabricated disposable electrode showed dynamic detection range, stability of the electrode, and selectivity and provides a new strategy by using green nanoparticles to detect selected bacteria from water.

Kinetics of Rifampicin Antibiotic Degradation Using Green Synthesized Iron Oxide Nanoparticles

Kinetics of Rifampicin Antibiotic Degradation Using Green Synthesized Iron Oxide Nanoparticles

Phyto-interactive impact of green synthesized iron oxide nanoparticles and Rhizobium pusense on morpho-physiological and yield components of greengram

The iron oxide nanoparticles (IONPs) prepared by green synthesis method using Syzigium cumini leaf extract was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The XRD confirmed the crystalline structure of green synthesized NPs measuring around 33 nm while SEM revealed its nearly spherical shape. Rhizobium species recovered from greengram nodules, identified by 16s rRNA gene sequencing as Rhizobium pusense produced 30% more exopolysaccharides (EPS) in basal medium treated with 1000 μg IONPs/ml. Compositional variation in EPS was observed by Fourier-transform infrared spectroscopy (FTIR). There was no reduction in rhizobial viability and no damage to bacterial membrane was observed under SEM and confocal laser scanning microscopy (CLSM), respectively. Effects of IONPs and R. pusense, used alone and in combination on the growth and development of greengram plants varied considerably. Plants grown with IONPs and R. pusence, used alone and in combination, showed a significant increase in seed germination rate, length and dry biomass of plant organs and seed components compared to controls. The IONPs in the presence of rhizobial strain further increased seed germination, plant growth, seed protein and pigments. Greater protein content (442 mg/g) was observed in seeds at 250 mg/kg of IONPs compared to control. Plants raised with mixture of IONPs plus R. pusense had maximum chlorophyll content (39.2 mg/g FW) while proline content decreased by 53% relative to controls. This study confirms that the green synthesis of IONPs from S. cumini leaf possess useful plant growth promoting effects and could be developed as a nano-biofertilizer for optimizing legume production.

Arsenic speciation, oxidation and immobilization in an unsaturated soil in the presence of green synthesized iron oxide nanoparticles and humic acid

While the availability of arsenic (As) in soil is well known to be highly correlated with the presence of iron (Fe) oxides and humic acid (HA) in the soil, the relationship between Fe oxides and HA and As species in the soil is less well understood. In this study, As speciation in an unsaturated soil in the presence of external HA and green synthesized Fe oxide nanoparticles (FeNPs) showed that As(V) was mainly distributed to the specifically-bound (F2), amorphous and poorly-crystalline hydrous oxides of Fe, Al (F3) and the well-crystallized hydrous oxides of Fe and Al (F4). While As(III). This was the major component in unsaturated soil, and was mainly distributed to F4 and the residual fraction (F5). As bound to F3 and F5 was most sensitive to the addition of HA and FeNPs, while HA/FeNPs treatment increased the F3-bound As(V); however, it decreased the F5-bound As(III). Nonetheless the effect of HA on As is completely different to the HA/FeNPs treatment. The increase of As(V) in F3 resulted from F5-bound As(III) oxidation when treated by HA/FeNPs. Cyclic voltammetry confirmed that HA and Fe3+/Fe2+ redox enhanced As(III) oxidation, while FTIR revealed that HA-bound As(III) was the least available fraction in the soil. Finally, a mechanism involving a combination of HA and FeNPs was proposed for explaining the redistribution of As species in the soil.

Effect of Green Synthesized Iron Oxide Nanoparticles Using Spinach Extract on Triton X-100-Induced Atherosclerosis in Rats.

The effect of iron oxide nanoparticles (FeONPs) synthesized using Spinacia oleracea leaf extract on Triton X-100-induced atherosclerosis in white Wistar rats was determined. FeONPs were characterized to determine their size, structure, composition, and shape. In vitro antioxidant activity of FeONPs against 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) was determined. Atherosclerosis was induced by intraperitoneal administration of 5% Triton X-100 (100 mg/kg body weight) for 14 days. Group 1 received standard rat chow and water. Group 2 received 100 mg/kg body weight of Triton X-100 and a standard diet. Group 3 received 100 mg/kg body weight of Triton X-100 followed by 20 mg/kg body weight of atorvastatin for 21 days. Groups 4, 5, and 6 received 100 mg/kg body weight Triton X-100 was followed by variable concentrations of 100, 300, and 500 µg/kg body weight FeONPs, respectively, for 21 days. Blood samples were analyzed for lipid, liver, antioxidant, and cardiovascular markers. Histopathology of the heart was also examined. Characterization revealed the amorphous nature, functional groups, and clustered topography of FeONPs. An upregulated antioxidant activity of FeONPs was observed in a dose-dependent manner. Administration of Triton X-100 showed elevated levels of lipid biomarkers except for high-density lipoprotein (HDL), which decreased in group 2 in comparison to group 1. Liver, antioxidant, and cardiovascular biomarkers all significantly increased. The structural alteration was observed in the heart tissue following histopathology examination. Administration of FeONPs significantly decreased all biomarkers and increased the level of HDL. Also, tissue architecture was restored. Our findings demonstrated that FeONPs were effective in ameliorating Triton X-100-induced atherosclerosis in rats.

Green synthesis of iron oxide nanoparticles usingCeratonia siliqua L.aqueous extract: improvement of colloidal stability by optimizing synthesis parameters, and evaluation of antibacterial activity against Gram-positive and Gram-negative bacteria

AbstractThis study focused on the colloidal stability enhancement of iron oxide nanoparticles synthesized using aqueous extract of theCeratonia siliqua L.(carob pod) by optimizing the synthesis parameters. The synthesis parameters were determined as the concentration of iron ions, the concentration of extract,pHof extract, temperature, stirring rate, and reaction time. The significance of the studied factors in controlling the particle size distribution of nanoparticles was quantitatively evaluated via analysis of variance (ANOVA). Iron oxide nanoparticles were produced with an average zeta potential of +41 ± 0.8 mV, hydrodynamic size of 78 ± 22 nm, and a polydispersity value of 0.42 ± 0.06, respectively. As a result of the stability study by measuring the zeta potential, it was determined that the colloidal stability was maintained for 3 months. Green iron oxide nanoparticles (gIONPs) showed inhibition zones of 24.27 ± 0.12 mm and 20.83 ± 0.11 mm in 250 mg/mL concentration against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial strains, respectively.S. aureuswas susceptible to the gIONPs according to the standard antibiotics of Cefotaxime (≥23 mm), Tetracycline (≥19 mm), Gentamicin (≥15 mm), and Cefoxitin (≥22 mm).E. coliwas susceptible to the gIONPs according to the standard antibiotics of Tetracycline (≥19 mm) and Gentamicin (≥15 mm), but showed resistance to the Cefotaxime (15–22 mm) and Cefoxitin (≤21 mm) standard antibiotics. This study suggests that the green synthesized iron oxide nanoparticles could be used as an antimicrobial agent and a promising candidate for usage in sensor, biomedical, and electronics applications for being in a highly stable structure.

Green synthesis of iron oxide nanoparticles using Amla seed for methylene blue dye removal from water

Aqueous seed extract of Amla was used for green synthesis of Iron oxide nanoparticles. Amla extract acted as a bio-reducing and stabilizing agent. Synthesized nanoparticles were characterized by Fourier Transform Infrared (FTIR) spectroscopy, Transmission Electron Microscopy (TEM) and X-ray Diffraction (XRD) techniques. TEM studies revealed average diameter of synthesized nanoparticles in the range of 4–5 nm. Stabilizing and capping agents were confirmed by FT-IR and the crystalline nature was confirmed by XRD. UV–Visible spectroscopy was used for monitoring Methylene Blue (MB) dye removal by green synthesized Iron oxide nanoparticles (IO NPs). Thus, it could be concluded that Amla seed extract can be used for the synthesis of iron oxide NPs and can be effectively used as a catalyst for the effective removal of toxic dyes.