Green Junglefowl Research Articles

THE BIRDS OF THE KANGEAN ISLANDS AND AN APPRAISAL OF THEIR CONSERVATION STATUS

The Kangean islands are a biologically poorly known archipelago situated in the Java Sea, Indonesia, approximately 120 km directly north of Bali. These islands host an avifauna comprising at least 13 endemic subspecies, two of which have by some sources recently been considered species. We combine historical published literature with our own surveys of the archipelago in 2007–2008, 2010 and 2023 to produce a complete list of the islands’ birds (n=140 species) and their distribution across the islands. We evaluate in more detail the conservation status of all endemic taxa, as well as several others we consider to be of concern. Despite its listing as Vulnerable on the IUCN Red List, the endemic Kangean Tit-babbler Mixornis prillwitzi is highly tolerant of habitat degradation and is among the islands’ least threatened birds. However, because of trapping, the archipelago’s endemic taxon of Red-breasted Parakeet Psittacula alexandri kangeanensis, and its populations of Green Junglefowl Gallus varius and Common Hill Myna Gracula religiosa are likely at risk of extirpation. The endemic taxon of White-rumped Shama Copsychus malabaricus, sometimes afforded species rank as ‘Kangean Shama’ C. nigricauda, may already be (at least functionally) extinct in the main archipelago. We recommend longer and more detailed surveys of the islands’ birds, and that an effective protected area is established on main Kangean island, which hosts the largest area of remaining forest in the archipelago and most of its endemic taxa.

Looking at the potential of HSP70 gene sequence in the germplasm of Indonesian green jungle fowl

Looking at the potential of HSP70 gene sequence in the germplasm of Indonesian green jungle fowl

Kajian Histokimia Sebaran Karbohidrat Netral Pada Usus Halus Ayam Hutan Hijau (Gallus Varius) Asal Pulau Alor

Green jungle fowl (Gallus varius) is a type of local chicken in Indonesia. Green jungle fowl are only found in Indonesia, which are spread from Java, Madura, Bali, Lombok, Sumba, Sumbawa, Flores and Alor. The small intestine consists of the duodenum, jejunum and ileum which function in the absorption of nutrients. Periodic Acid Schiff (PAS) is a special staining method that aims to see the characteristics of neutral carbohydrates in cells or body tissues. A positive PAS staining reaction is indicated by the presence of a magenta-red color in the examined tissue. This study aims to determine the distribution of neutral carbohydrates in the small intestine of the green jungle fowl (Gallus varius) from Alor Island. Small intestine samples were taken from 3 green jungle fowl collected in Alor District. Microscopic observation of small intestine tissue that has been stained with PAS stain. The results showed that the small intestine of the green jungle fowl consists of the duodenum, jejunum and ileum. Neutral carbohydrates are distributed throughout the tunica duodenum, jejunum and ileum with varying intensity. Moderate intensity (++) was seen in goblet cells and liberkuhn's crypts. Weak intensity (+) is seen on the surface of the epithelium, lamina propria, lamina muscularis mucosa, tunica submucosa, tunica muscularis, and tunica serosa.

Genetic Structure of Gallus varius Based on Middle-Lower Section of Control Region mtDNA

Gallus varius, an indigenous bird species of Indonesia, demonstrates significant genetic diversity. The genetic diversity assessment in G. varius encompassed both mitochondrial DNA (mtDNA) and nuclear DNA, with the mtDNA analysis primarily centered on domain I of the control region. This study aimed to describe the genetic diversity and structure of G. varius inhabiting Java, Madura, Lombok and Sumbawa based on the middle-lower part of control region mtDNA. Genomic DNA was extracted from the calamus tip of feather, then the middle-lower part of control region was amplified and sequenced using two pairs of primers. In the examined control region, spanning from 944 to 1,008 bp, 13 bp of nucleotide variation was observed, with nucleotide diversity at 0.0021. Between G. varius samples and the reference (NC_007238.1), a total of 7 haplotypes were identified, 5 unique and 2 shared haplotypes, with haplotype diversity at 0.7692. The substantial diversity of haplotypes in this study and two previous study suggests that the genetic diversity of G. varius has remained stable over the past two decades. Additionally, genetic distance data indicate there is multiple G. varius subspecies, and the haplotype network accentuates signs of population differentiation.

Hundreds of independent midsize deletions mediate DNA loss in wild relatives of Red Jungle Fowl

Small and midsize deletions and insertions (InDels) are major events that play a crucial role in the evolution of genome size and contribute to the genetic and phenotypic diversity of species. In recent years, considerable attention has been given to studying small indels associated with various developmental, growth, and production traits in domestic chicken breeds. Additionally, small and midsize indels have been studied between chicken and phylogenetically more distant species such as duck, turkey, rock pigeon, and other passerine birds. However, the investigation of small and midsize deletions in the wild relatives of chickens has been relatively overlooked until now. To address this gap, our study aimed to identify the presence and distribution of midsize deletions (> 1 Kb) in the wild relatives of chickens. We conducted a comparative genomic analysis using high-quality genomic data from four species belonging to the Gallus genus. Our analysis revealed the existence of >125 midsize deletions in the three other species compared to Gallus gallus (red junglefowl). These midsize deletions were found to be distributed in intergenic regions and within introns of various protein-coding genes but not in the exonic regions of protein-coding genes. Furthermore, we observed a trend between the number of midsize deletions and the phylogenetic distance in the phylogeny of the Gallus genus. The most ancestral species, Gallus varius (green junglefowl), exhibited the highest deletions, followed by Gallus lafayettii (Ceylon junglefowl) and Gallus sonneratii (grey junglefowl). Some protein-coding genes harboring deletions in their introns and upstream regions were associated with body development, production, growth traits, abdominal fat deposition, behavioral patterns such as stress, fear, anxiety, plumage color, and adaptation to extreme climatic conditions. Our study finds that the midsize deletions identified in wild relatives of red junglefowl contribute <1% of DNA loss with a rate of 8–44 Kb/My during the evolution of the Gallus genus.

Detection of long terminal repeat loci derived from endogenous retrovirus in junglefowl using whole-genome sequencing

Endogenous retroviruses (ERVs) are genetic elements present in the genome that retain traces of past viral infections. Characterization of ERVs can provide crucial insights into avian evolution. This study aimed to identify novel long terminal repeat (LTR) loci derived from ERVs (ERV-LTRs) absent in the reference genome using whole-genome sequencing data of red junglefowl, gray junglefowl, Ceylon junglefowl, and green junglefowl. In total, 835 ERV-LTR loci were identified across the four Gallus species. The numbers of ERV-LTRs loci detected in red junglefowl and its subspecies gray junglefowl, Ceylon junglefowl, and green junglefowl were 362, 216, 193, and 128, respectively. The phylogenetic tree was congruent with previously reported trees, suggesting the potential for inferring relationships among past junglefowl populations from the identified ERV-LTR loci. Of the detected loci, 306 ERV-LTRs were identified near or within the genes, and some were associated with cell adhesion. The detected ERV-LTR sequences were classified as endogenous avian retrovirus family, avian leukosis virus subgroup E, Ovex-1, and murine leukemia virus-related ERVs. In addition, the sequence of the EAV family was divided into four patterns by combining the U3, R, and U5 regions. These findings contribute to a more comprehensive understanding of the characteristics of junglefowl ERVs.

SCREENING POLE OF GREEN FOREST CHICKEN (Gallus varius) AND ITS POTENTIAL AS A TEACHING SOURCE OF BIOCULTURAL DIVERSITY: A CASE STUDY IN BUKIT DEMULIH BANGLI INDIGENOUS FOREST AREA

Green jungle fowl (Gallus varius) is widespread in nature and community forests. This study aims to identify distribution patterns, habitat profiles, and the potential of green jungle fowl (GJF) as a source of biological learning based on a biocultural approach (biodiversity, cultural diversity, and language diversity). The case study was used for extensive bird exploration in a customary forest, Hutan Adat Bukit Demulih Bangli (HABD), from February to June 2022. Data on distribution patterns were collected with a combination of free-range, line transect, and point-count techniques and habitat profiles with physiognomy methodology. Meanwhile, document analysis and the concept map were used to demonstrate the potential of the GJF in HABD as a source of learning. The results revealed that GJF was unequally distributed, with a population at each observation point ranging from 2 to 10. The bird prefers forested areas dominated by shrubs with several trees and, therefore, has an open canopy that allows sunlight to reach the forested floor. Local knowledge and practices in the preservation of GJF and HABD are excellent resources for learning various biological materials and concepts in the context of biodiversity, culture, and language.

Why did the chicken cross the Wallace Line? Archeological evidence suggests human‐mediated dispersal of Gallus to Flores first occurred at least ~2.25 ka cal. BP

AbstractDomesticated chickens (Gallus gallus domesticus) are a dominant part of the global human diet. Although the early domestication history of this species remains disputed, Mainland Southeast Asia (MSEA) is assumed to have been the initial domestication center. The eastward spread of chickens into Island Southeast Asia (ISEA) and the Pacific is typically attributed to human‐mediated dispersals. Chicken remains are relatively common at Pacific Neolithic sites but are extremely rare in the archaeological records of MSEA and ISEA. Therefore, the exact routes and timing of the human‐mediated spread of chickens from their native range in MSEA into the Pacific remain questions of interest. Here, we present the earliest evidence of Gallus on the Indonesian island of Flores at Liang Bua. This site has yielded an extensive stratigraphic sequence that spans from ~190,000 calendar years (ka) ago until the present and includes dense accumulations of faunal remains. Twelve bones from the cave's Holocene deposits have been identified as Gallus. The oldest remains, a right and left coracoid, were each directly dated to ~2,250 calibrated radiocarbon years before present (ka cal. BP), whereas the youngest Gallus elements are ~0.3 ka old. Although wild Green Junglefowl (Gallus varius) and Red Junglefowl (Gallus gallus) are found on Flores today, the absence of either of these species in deposits at Liang Bua older than ~2.5 ka as well as the size and shape of the oldest coracoids suggests that these remains likely represent domesticated G. gallus. This is the first evidence for domesticated chickens in the Neolithic of Flores and the first directly dated Gallus remains in Wallacea. The absence of chickens in the fossil record of ISEA suggests that Red Junglefowl (and perhaps Green Junglefowl also) reached Wallacea via human‐mediated dispersal(s) at least ~2.25 ka cal. BP.

Gambaran Anatomi dan Histologi Usus Besar Ayam Hutan Hujau (Gallus varius) Asal Pulau Alor

Green jungle fowl (Gallus varius) is one of Indonesia's endemic poultry species, which is often used by the community to produce high-value ornamental birds. This study aims to determine the anatomical morphology and histology of the large intestine of green jungle fowl. Colon samples were taken from 3 green jungle fowl collected in Alor Regency. The obtained samples were observed macroscopically and then fixed using 10% formalin, then processed into histological preparations and stained with hematoxylin-eosin (HE). The results showed that the large intestine of the green jungle fowl consisted of a pair of cecum and a rectum. The location of the cecum and rectum of green jungle fowl is the same as that of poultry in general, which is located in the peritoneal cavity, adjacent to other intestinal segments. Each cecum consists of 3 parts, namely proximal, corpus, and apex. The cecum is pale red in color with a soft consistency. The average cecum length of the green jungle fowl is 10.9 cm. The rectum is the last segment of the intestine in the form of a straight channel that connects the ileum and cloaca. The rectum is pale red in color, soft in consistency and has thicker walls than the rest of the intestine. The average length of the rectum of the green jungle fowl is 3.9 cm. Histologically the cecum and rectum walls of green jungle fowl are composed of tunica mucosa, tunica submucosa, tunica muscularis and tunica serosa. The wall layer of the large intestine of green jungle fowl is generally the same as other birds, but differs in the muscularis tunica, namely circular smooth muscle fibers located on the outside and longitudinal smooth muscle on the inside.

Histomorfologi Dan Histomorfometri Otot Ayam Hutan Hijau (Gallus Varius) Asal Pulau Alor

Green jungle fowl is one of the endemic animals in Indonesia. One of the distribution areas of green jungle fowl in East Nusa Tenggara is the island of Alor. Until now there has been no research conducted to determine the histological structure of the green jungle fowl so that this study was conducted with the aim of knowing the histological structure and muscle histomorphometry of the green jungle fowl. The research samples were pectoralis and bicep femoris muscles taken from three green jungle fowl in Kalabahi, Alor Regency. Muscle tissue was fixed using 10% formalin, histological preparations were made and stained with hematoxylin and eosin (HE). The results showed that the muscle histomorphology in the transverse section showed that the skeletal muscle of the green jungle fowl was composed of muscle fibers in a polygonal shape with many nuclei at the edges and connective tissue. No intramuscular fat cells were found. In the longitudinal section, it consists of muscle fibers with a light dark line pattern, the cell nucleus is elongated at the edges and connective tissue. The results of muscle histomorphometry, namely the diameter of the muscle fibers, the diameter of the fasciculus and the thickness of the connective tissue in the bicep femoris muscle area were higher than the pectoralis muscle area. The number of muscle fibers in each fasciculus in the bicep pectoralis muscle area is more than in the bicep femoris muscle area. Muscle histomorphometry is influenced by species, breed/race, age, diet, activity level and anatomical location.

Short Communication: The use of lactose-astaxanthin to maintain the quality of green junglefowl frozen semen

Abstract. Bebas W, Agustina KK. 2022. Short Communication: The use of lactose-astaxanthin to maintain the quality of green junglefowl frozen semen.Biodiversitas 23: 5759-5764. The Green Junglefowl is Indonesian germplasm that is at risk of population decline. Therefore, efforts are needed to protect its existence and survival. Cryopreservation of spermatozoa in the form of frozen semen is required to preserve this germplasm. This research aims to determine the effect of lactose-astaxanthin in preventing DNA fragmentation and intact plasma membrane of Green Junglefowl spermatozoa during the freezing process. A completely randomized design with a 2x4 factorial pattern, including two treatment groups and four dimethylsulfoxide (DMSO) concentration factors, was used. The treatment groups are T1 and T2, consisting of egg yolk phosphate diluent and egg yolk phosphate diluent added with a combination of 0.6% lactose-0.004% astaxanthin, respectively. The four DMSO concentration factors were 4%, 6%, 8%, and 10% respectively. Subsequently, each treatment was repeated four times. The DNA fragmentation and intact plasma membrane of spermatozoa were observed, and the data obtained afterward were analyzed for variance, followed by the Ducan test. The results showed that the addition of 0.6% lactose-0.004% astaxanthin with 6% DMSO had a significantly better effect (P&lt;0.05) on the quality of post-thawing spermatozoa with 19.75±0.96% DNA fragmentation and 53.75±3.86% intact plasma membranes. Therefore, it is recommended that a combination of 0.6% lactose-0.004% astaxanthin at 6% DMSO concentration should be added to Green Junglefowl semen during the freezing process to prevent DNA fragmentation and intact plasma membranes.

Anatomical and Histological Morphology of The Esophagus and Proventriculus in Green Jungle Fowl (Gallus varius) from Alor Island

Green jungle fowl (Gallus varius) is a species of jungle fowl endemic Indonesia. This study aims to find out the anatomical and histomorphologIcal of esophagus and proventriculus of green jungle fowl from Alor island. Samples of esophagus and proventriculus were taken from 3 green jungle fowl collected in Alor Regency. Macroscopic study observations include size, location, shape, color and consistency, then sample is fixated in a 10% formalin and made histology preparations as well as haemotoxilin-eosin staining. The results showed that the cervical and thoracic esophagus length is 9.8 ± 0.43 cm and 5.5 ± 0.24 cm, and diameters are 0.55 ± 0.11 cm and 0.6 ± 0.22 cm. The green jungle fowl esophagus is pale red, soft constency,and has a smooth outer surface. Histologically, the walls of esophagus are consist of tunica mucosa, tunica submucosa, tunica muscularis ekterna, and tunica adventisia. Proventriculus green jungle fowl length is 3.23 ± 0.21 cm, diameter 1.83 ± 0.24 cm, and weight 2 ± 0 g. The proventriculus is fusiform, pale red in color, and has denser consistency than esophagus. Proventriculus walls are composed of tunica mucosa, tunica submucosa, tunica muscularis ekterna and tunica serosa.

Response of bird community to gradual landscape transformation in Darmaga campus, IPB University, Bogor, Indonesia

When a landscape gradually transforms, the bird community that inhabits the landscape will also adapt and change accordingly. Long-term data on the bird-habitat relationship, however, is still lacking in the sub-urban tropical areas. The objective of this paper was to analyze the response of bird community along with the gradual transformation of the landscape. Darmaga Campus of IPB University (± 2.67 km2) was selected as the study area due to its landscape transformation for campus development/construction, and the availability of bird monitoring data. Data on landscape transformation were gathered from the IPB building construction book published in 2017, and bird community (1982-2020) were gathered from published reports and papers. During the observed period, Darmaga Campus that initially consisted mostly of old rubber plantation has been gradually transformed into various academic-related uses, such as some areas for buildings dan field laboratories. From 1982 to 2020, the cumulative bird species in the study area at least was 124 species. During the observed years, the bird community was highly dynamics (bird species number in 1982 = 41 species, 1985 = 39 species, 1986 = 68 species, 1991 = 68 species, 2001 = 39 species, 2003 = 72 species, 2013 = 52 species, 2020 = 99 species). New incoming species were Chestnut-winged Cuckoo, Asian Brown Flycatcher, Tiger Shrike, and babblers (e.g., Black-capped Babbler); Brown Shrike, White-rumped Shama, Oriental Magpie-Robin and Green Junglefowl have not been observed or have become increasingly rare in the last ten years. Meanwhile, Red-breasted Parakeet, Fulvous-breasted Woodpecker, Sunda Pygmy Woodpecker, Black Drongo, and Sooty-headed Bulbul are always present species. Bird species such as Orange-headed Thrush, White-rumped Shama, White-browed Shortwing, and Velvet-fronted Nuthatch are species that entered to rarely found species. Gradual landscape transformation, from an old rubber plantation into a mosaic of small patches of human-made ornamental plants, shrub, and tree plantation, turns out to be beneficial for some bird species, but could also disadvantageous for others, causing a dynamic bird community composition.

Unraveling the history of the genus Gallus through whole genome sequencing

The genus Gallus is distributed across a large part of Southeast Asia and has received special interest because the domestic chicken, Gallus gallus domesticus, has spread all over the world and is a major protein source for humans. There are four species: the red junglefowl (G. gallus), the green junglefowl (G. varius), the Lafayette’s junglefowl (G. lafayettii) and the grey junglefowl (G. sonneratii). The aim of this study is to reconstruct the history of these species by a whole genome sequencing approach and resolve inconsistencies between well supported topologies inferred using different data and methods.Using deep sequencing, we identified over 35 million SNPs and reconstructed the phylogeny of the Gallus genus using both distance (BioNJ) and maximum likelihood (ML) methods. We observed discrepancies according to reconstruction methods and genomic components. The two most supported topologies were previously reported and were discriminated by using phylogenetic and gene flow analyses, based on ABBA statistics. Terminology fix requested by the deputy editor led to support a scenario with G. gallus as the earliest branching lineage of the Gallus genus, instead of G. varius. We discuss the probable causes for the discrepancy. A likely one is that G. sonneratii samples from parks or private collections are all recent hybrids, with roughly 10% of their autosomal genome originating from G. gallus. The removal of those regions is needed to provide reliable data, which was not done in previous studies. We took care of this and additionally included two wild G. sonneratii samples from India, showing no trace of introgression. This reinforces the importance of carefully selecting and validating samples and genomic components in phylogenomics.

The wild species genome ancestry of domestic chickens

BackgroundHybridisation and introgression play key roles in the evolutionary history of animal species. They are commonly observed within several orders in wild birds. The domestic chicken Gallus gallus domesticus is the most common livestock species. More than 65 billion chickens are raised annually to produce meat and 80 million metric tons of egg for global human consumption by the commercial sector. Unravelling the origin of its genetic diversity has major application for sustainable breeding improvement programmes.ResultsIn this study, we report genome-wide analyses for signatures of introgression between indigenous domestic village chicken and the four wild Gallus species. We first assess the genome-wide phylogeny and divergence time across the genus Gallus. Genome-wide sequence divergence analysis supports a sister relationship between the Grey junglefowl G. sonneratii and Ceylon junglefowl G. lafayettii. Both species form a clade that is sister to the Red junglefowl G. gallus, with the Green junglefowl G. varius the most ancient lineage within the genus. We reveal extensive bidirectional introgression between the Grey junglefowl and the domestic chicken and to a much lesser extent with the Ceylon junglefowl. We identify a single case of Green junglefowl introgression. These introgressed regions include genes with biological functions related to development and immune system.ConclusionsOur study shows that while the Red junglefowl is the main ancestral species, introgressive hybridisation episodes have impacted the genome and contributed to the diversity of the domestic chicken, although likely at different levels across its geographic range.

Various Gallus varius hybrids: variation in junglefowl hybrids and Darwin's interest in them

Hybrids between Green Junglefowl Gallus varius and domestic fowl G. gallus domesticus confused several 19th-century ornithologists. The plumage of these hybrids is so unlike the colours and patterns of either of the parent species that they were considered to be distinct species: G. aeneusTemminck, 1825; G. temminckiiGray, 1849; and G. violaceusKelsall, 1891. Darwin wanted to understand if G. aeneus and G. temminckii were hybrids or species, as part of his research on the origin of the domestic chicken. His view was that all domesticated fowl have a single wild ancestor, Red Junglefowl G. gallus (formerly G. bankiva). A hybrid specimen now present in the bird collection of the Natural History Museum at Tring played an important role in Darwin's reasoning and, although the conclusions he drew from this specimen were incorrect, his single-ancestor origin theory for domesticated fowl stands.‘These hybrids were at one time thought to be specifically distinct, and were named G. aeneus. Mr. Blyth and others believe that the G. Temminckii is a similar hybrid' (Darwin 1868a: 234–235).

Struktur Populasi Genetik Ayam Hutan Hijau Menggunakan Sekuen Hypervariable 1 D-Loop DNA Mitokondria

Thirty-three individuals from four populations of green junglefowl (Gallus varius) were collected throughout Indonesia: 14 from Central Java, 4 from Eastern Java, 3 from Sumbawa island and 12 from Flores island. The mitochondrial DNA D-loop the samples were analysed for sequence diversity. Twenty-five haplotypes with 28 polymorphic sites were identified within the first hypervariable-1 fragment (397 bp) of the D-loop. Fu’s Fs value was -25.96 (all samples, P=0), indicating high genetic diversity and population expansion; a conclusion supported by a neighbor–joining analysis of the haplotypes. Though sample size per population varied between 3 and 14, the Fs values for the four populations, between -2.20 and -10.76, were all significant (P=0). Only one haplotype was shared between three populations (Central Java, Sumbawa island, and Flores island) by a total of seven individuals. Within populations, only three haplotypes were shared by three individuals. The remaining haplotypes were unique, indicating genetic differentiation between populations as confirmed by significant pairwise Fst values at P=0.05 in four out of the six population pairs (except two pairs of Central Java &amp; Sumbawa island and Flores island &amp; Sumbawa island). The diversity pattern observed at the mtDNA of the green junglefowl provides a baseline which may help to understand the recent population expansions of domesticated chickens from multiple centres of domestications. Our observations also suggest careful interpretation of the results of genetic characterization may be needed when applied to the management and conservation of species like the green junglefowl. As in other multiparous birds and mammals with a short reproductive interval, green junglefowl may have established distinct genetic entities in metapopulations across its geographic distribution.

Complete mitochondrial genome of Sri Lankan Junglefowl (Gallus lafayetti) and phylogenetic study

The complete mitochondrial genomes of two Sri Lankan junglefowl (Gallus lafayetti: CJF) individuals were sequenced by using next-generation sequencing technique. Samples were collected from Rathnapura and Pelmadulla areas in Sri Lanka. The complete mitochondrial DNA is 16,839 bp in length, with a typical mitogenome structure composed of a non-coding control region, 22 tRNA, two rRNA, and 13 protein-coding genes. Overall base composition is 30% A, 23.9% T, 32.3% C, and 13.6% G indicating high content of 54.0% A + T for both individuals. Phylogenetic analysis reveals that CJF samples cluster with the clade of the green junglefowl (Gallus varius) and red junglefowl (Gallus gallus) than to grey junglefowl (Gallus sonerattii: GyJF). This result can be subsequently used to provide essential information for junglefowl evolution.

Species Richness and Birds Conservation Priority in Mount Rinjani Areas, Lombok

&lt;p&gt;Mount Rinjani area on the island of Lombok is unique in bioecology. It provides habitats for many species of birds, especially birds of Wallacea. Several studies on birds in this area have been done, but the conservation priority of these birds has not been determined. Based on this condition, the purpose of this research is to determine the priority of birds conservation in mount Rinjani areas. Exploration about the spesies of birds was started from Senaru Village to Segara Anak Lake. Abundance of each bird species was estimated using point count. Data analysis to determine the conservation priority used indicators that consist of endemicity, population status, species threatened and species management. The results showed that the birds species in mount Rinjani areas are composed of 32 species belonging to 20 families. Bird species that have high abundance were &lt;em&gt;Amandava amandava &lt;/em&gt;(15.94%), &lt;em&gt;Zosterops Montanus&lt;/em&gt; (15.70%), and &lt;em&gt;Parus major&lt;/em&gt; (11.32%), while &lt;em&gt;Ptilinopus melanospila&lt;/em&gt; and &lt;em&gt;Anthus novaeseelandiae&lt;/em&gt; were in low abundance (0.23%). Analysis birds conservation priority in mount Rinjani areas indicates that &lt;em&gt;Otus jolandae&lt;/em&gt; has the highest score (65) and should be the first priority for the conservation, while &lt;em&gt;Philemon buceroides&lt;/em&gt; and &lt;em&gt;Gallus varius&lt;/em&gt; with the same score (60) are the second priority.&lt;em&gt; &lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;How to Cite&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;Hadiprayitno, G., Mertha, I. G., &amp;amp; Ilhamdi, M. L. (2016). Species Richness and Birds Conservation Priority in Mount Rinjani Areas. &lt;em&gt;Biosaintifika: Journal of Biology &amp;amp; Biology Education&lt;/em&gt;, 8(3), 270-277. &lt;/p&gt;

Multiple maternal origins of Indonesian crowing chickens revealed by mitochondrial DNA analysis

The utilization of Indonesian crowing chickens is increasing; as such, assessing their genetic structures is important to support the conservation of their genetic resources. This study analyzes the matrilineal evolution of Indonesian crowing chickens based on the mtDNA displacement loop D-loop region to clarify their phylogenetic relationships, possible maternal origin, and possible routes of chicken dispersal. The neighbor-joining tree reveals that the majority of Indonesian crowing chickens belong to haplogroups B, D, and E, but haplogroup D harbored most of them. The Bayesian analysis also reveals that Indonesian crowing chickens derive from Bekisar chicken, a hybrid of the green junglefowl, suggesting the possible contribution of green junglefowl to chicken domestication. There appear at least three maternal lineages of Indonesian chicken origins indicated by the median network profile of mtDNA D-loop haplotypes, namely (1) Chinese; (2) Chinese, Indian, and other Southeast Asian chickens; and (3) Indian, Chinese, Southeast Asian, Japanese, and European chickens. Chicken domestication might be centered in China, India, Indonesia, and other Southeast Asian countries, supporting multiple maternal origins of Indonesian crowing chickens. A systematic breeding program of indigenous chickens will be very important to retain the genetic diversity for future use and conservation.