Green Aphid Research Articles

Wing Plasticity Is Associated with Growth and Energy Metabolism in Two Color Morphs of the Pea Aphid.

The pea aphid, Acyrthosiphon pisum, is a major pest of legume crops, exhibiting distinct polymorphism in terms of wings and body color. We found that, under crowded conditions, the red morph A. pisum produced more winged offspring than the green morph. The signaling pathways involved in aphid wing determination, like insulin and ecdysone, also play important roles in regulating growth, development, and metabolism. Thus, here, we examined the association between the wing-producing ability and the growth rate, development time, reproductive capacity, and energy metabolism in these two color morphs. The growth rate of red morphs was significantly higher than that of green morphs, whereas green morphs produced more offspring during the first 6 days of the adult stage. Red morphs accumulated higher levels of glycogen and triglycerides and consumed more triglycerides during starvation; however, green aphids consumed more trehalose during food deprivation. Red aphids exhibited stronger starvation tolerance, possibly due to their higher triglyceride catabolic activity. Furthermore, the expression levels of genes involved in the insulin pathway, glycolysis, and lipolysis in red aphids were higher than those in green aphids. These results suggest that the wing-producing ability of the pea aphid may be associated with its growth and metabolism, which may be due to the shared regulatory signaling pathways.

Morphological and Molecular Identification of Novel Green Peach Aphids (Myzus persicae) (Hemiptera: Aphididae) and Their Microbiome Diversity in Taif Governorate

Background: The green aphid (Myzus persicae) found in Taif Governorate is a sap-sucking insect that occurs globally. These insects target all the plant parts in both their nymphal and adult stages, causing them to discolour and dry out. Methods: In this study, 250-300 aphids were widely collected from rose plants in different locations in the Taif Governorate. These insects were identified using morphological traits and mitochondrial gene sequencing. Results: According to morphological traits, these samples belonged to the species Myzus persicae. Considering that aphid species are very similar morphologically, the identification of samples that were similar to more than one species was performed using their genetic characteristics. Aphid strain characterization through Sanger sequencing with multilocus sequence typing (MLST) and phylogenetic analysis revealed significant diversity, and comparing mitochondrial gene sequences of aphids with extant sequences in GenBank showed high similarity and a new strains was recorded in the NCBI database for the first time in Taif Governorate. For microbiome isolation, five bacterial species were isolated from M. persicae (Bacillus spp., Serratia spp., Staphylococcus, Micrococcus spp., and Escherichia coli). Our results showed a significant correlation between M. persicae and microbial communities. Future research should focus on discovering new strains of M. persicae and understanding the eco-evolutionary patterns of aphid-symbiont interactions in the Taif Governorate, particularly in biological control.

Some Physiological Effects of Nanofertilizers on Wheat-Aphid Interactions.

The increasing use of nanofertilizers in modern agriculture and their impact on crop yield and pest management require further research. In this study, the effects of nano-Fe, -Zn, and -Cu (which are synthesized based on nanochelating technology), and urea (N) fertilizers on the antioxidant activities of wheat plants (cv. Chamran), and the wheat green aphid Schizaphis graminum (Rondani) are investigated. The authors observed the highest levels of phenolics in non-infested nano-Zn-treated plants (26% higher compared with control). The highest H2O2 levels are in the infested and non-infested nano-Zn-treated and infested nano-Fe-treated plants (in infested nano-Zn and nano-Fe treated plants, 18% and non-infested nano-Zn-treated plants, 28% higher compared with control). The highest peroxidase (POX) activity is observed in the infested and non-infested N-treated and non-infested water-treated plants (almost 14%, 37%, and 46% higher than control, respectively). The lowest activity is in the infested plants' nano-Zn and -Fe treatments (almost 7 and 5 folds lower compared to the control, respectively). The highest and lowest catalase (CAT) activity are in the infested N-treated plants (almost 42% higher than control) and water-treated plants, respectively. The infested nano-Zn, -Fe, -Cu and Hoagland-treated plants showed the highest superoxide dismutase (SOD) activity. Regarding the antioxidant enzyme activities of S. graminum, the highest POX activity is in the nano-Cu treatment (more than two folds higher compared with control); the highest CAT and SOD activities are in the nano-Cu and -Zn treatments. It can be concluded that the application of nanofertilizers caused increasing effects on the wheat plant's antioxidant system and its resistance to S. graminum.

Sistem Pakar Diagnosa Hama Penyakit Tanaman Tomat Menggunakan Metode Forward Chaining

The tomato (Solanum lyco persicum) is a plant from the solanceae family, native to Central and South America, from Mexico and Peru. Tomatoes have a short life cycle and height from I to 3 meters. Currently, farmers often ignore pests and diseases of tomatoes due to lack of knowledge. They also assessed that it finally happened at the time of planting, experts on pests and diseases of tomatoes were eliminated, there was a lack of socialization from agricultural officials about pests and diseases in tomatoes, but they did not know how to control them and to eliminate diseases quickly or decrease productivity. This study uses the Forward Chaining method, which is a description that starts from known facts and then is matched with IF and IF-THEN rules. Pests and diseases with a high prevalence in tomatoes are pests. Some pests are: fruit caterpillars, earthworms, green aphids, white flies, fruit flies (bractrotera) and further diseases namely: Fusarium wilt, leaf spot disease, bacterial spots, fruit rot. This expert system application provides information on tomato diseases and disease diagnosis, including control solutions that can be used to reduce the risk of damage to tomato plants. This study aims to build an expert system application that can diagnose tomato plant pests and diseases using the forward chaining method. This application is expected to help the community in diagnosing the types of tomato plant pests. The implementation of the application of an expert system for pests and diseases of tomato plants is expected to provide easy access for users and the wider community, through the use of web-based media.

Differential regulation of the Tor gene homolog drives the red/green pigmentation phenotype in the aphid Myzuspersicae

In some aphid species, intraspecific variation in body colour is caused by differential carotenoid content: whilst green aphids contain only yellow carotenoids (β-, γ-, and β,γ-carotenes), red aphids additionally possess red carotenoids (torulene and 3,4-didehydrolycopene). Unusually, within animals who typically obtain carotenoids from their diet, ancestral horizontal gene transfer of carotenoid biosynthetic genes from fungi (followed by gene duplication), have imbued aphids with the intrinsic gene repertoire necessary to biosynthesise carotenoids. In the pea aphid, Acyrthosiphon pisum a lycopene (phytoene) desaturase gene (Tor) underpins the red/green phenotype, with this locus present in heterozygous form in red individuals but absent in green aphids, resulting in them being unable to convert lycopene into the red compounds 3,4-didehydrolycopene and torulene. The green peach aphid, Myzus persicae, separated from the pea aphid for ≈45MY also exists as distinct colour variable morphs, with both red and green individuals present. Here, we examined genomic data for both red and green morphs of M. persicae and identified an enlarged (compared to A. pisum) repertoire of 16 carotenoid biosynthetic genes (11 carotenoid desaturases and five carotenoid cyclase/synthase genes). From these, we identify the homolog of A. pisum Tor (here called carotene desaturase 2 or CDE-2) and show through 3D modelling that this homolog can accommodate the torulene precursor lycopene and, through RNA knockdown feeding experiments, demonstrate that disabling CDE-2 expression in red M. persicae clones results in green-coloured offspring. Unlike in A. pisum, we show that functional CDE-2 is present in the genomes of both red and green aphids. However, expression differences between the two colour morphs (350–700 fold CDE-2 overexpression in red clones), potentially driven by variants identified in upstream putative regulatory elements, underpin this phenotype. Thus, whilst aphids have a common origin of their carotenoid biosynthetic pathway, two aphid species separated for over 40MY have evolved very different drivers of intraspecific colour variation.

EXPERT SYSTEM FOR DIAGNOSING TOMATO PESTS AND DISEASES USING FORWARD CHAINING METHOD

Tomato (solanum lyco persicum) is a plant from the solanceae family, native to Central and South America, from Mexico and Peru. Tomatoes have a short life cycle and a height ranging from 1 to 3 meters. Currently, farmers often experience disturbances from pests and diseases of tomatoes due to a lack of knowledge. They do not know how to control it and to eliminate pests that reduce productivity. In identifying diseases in tomato plants we use the Forward Chaining method which is a description that starts from knowing the facts and then matching them with IF and IF-THEN rules. Diseases with a high prevalence in tomatoes are: fruit worms, earthworms, green aphids, white flies, fruit flies (bractrotera) and the diseases are: fusarium wilt, leaf spot disease, bacterial spot, fruit rot. This expert system application provides tomato disease information and disease diagnosis, including control solutions that can be used to reduce the risk of tomato crop damage
 

Settling Preference of Two Coexisting Aphid Species on the Adaxial and Abaxial Surfaces of Walnut Leaves.

Walnut dusky-veined aphid Panaphis juglandis (Goeze) and walnut green aphid Chromaphis juglandicola (Kaltenbach) cause economic losses and co-occur on walnut trees, but they have separate niche. Panaphis juglandis feeds on the upper (adaxial) surface of leaves while C. juglandicola feeds on the lower (abaxial) surface. Field surveys and controlled experiments in the field and laboratory were conducted to determine microhabitat selection by P. juglandis and C. juglandicola and the factors associated with this behavior. In the field, the two aphid species colonized a leaflet as follows: P. juglandis only, 16.5%; C. juglandicola only, 44.5%; and both species on same leaflet, 39%. C. juglandicola settled on the abaxial surface earlier than P. juglandis settled on the adaxial surface. P. juglandis showed the highest reproduction rate when they were in the erect position on the adaxial surface. C. juglandicola exhibited the highest reproduction rate when they were inverted and on the abaxial surface. Under a light intensity of 50,000 lux, 60.5% of C. juglandicola remained on the illuminated surface, while P. juglandis did not move from the illuminated surface. Through field and laboratory experiments, we found that P. juglandis preferred to settle on the adaxial surface and C. juglandicola preferred to settle on the abaxial surface. Leaf surface, gravity, and light were three physical factors affecting microhabitat selection by the two aphid species but light intensity was the key factor. This information will help to better understand the habitats of two aphid species, which may be helpful for walnut aphids management strategies such as the usage of insecticides option and spraying.

Influence of Larval Diet on Pupal Period and Size of Cocoon of Chrysoperla zastrowi sillemi (Esben-Peterson)

A study was conducted in the Biocontrol Laboratory, Department of Entomology, College of Agriculture, OUAT, Bhubaneswar, Odisha during 2020-21 to record the influence of larval diets on pupal period and size of cocoon of Chrysoperla zastrowi sillemi (Esben-Peterson). Six larval diets i.e. T1 (Purple aphid, Aphis craccivora Koch.), T2 (Green aphid, Myzus persicae (Sulzer)), T3 (Mustard aphid, Lipaphis erysimi (Kalt.)), T4 (Papaya mealy bug, Paracoccus marginatus Williams and Granara de Willink), T5 (Pink mealy bug, Maconellicoccus hirsutus (Green)) and T6 (Corcyra cephalonica Stainton 1st instar larva) were tested along with one control (T7= Corcyra cephalonica egg). T1 was detected as the best larval diet which produced maximum pupal period of 8.90 days with 16.80% increased over control. Maximum cocoon diameter was also recorded in T1 i.e. 3.28 mm with 11.95% increased over control. T1 was closely followed by T3 with respect to pupal period (8.74 days with 14.70% increase over control) and diameter of cocoon (3.20 mm with 9.22% increase over control) of C. zastrowi sillemi.

Why Using Biological Control Approaches Against Sugar Beet Aphid Pests Will Not Control Virus Yellows – a Post-script

As an advocate of natural pest control wherever possible, the author has no quarrel with Professor Loxdale?s appeal for research funders to consider enhancing the resources already available in the natural environment. In an ideal world, this would be the most acceptable method for producing healthy crops for our benefit, not to mention encouraging biodiversity within the landscape. However, in the real world, a more pragmatic approach is sometimes required to avoid catastrophic yield losses, such as those which occurred in sugar beet in the mid 1970s, and most recently in 2020, when it was estimated that 25% of sugar beet yields were lost to the aphid-transmitted yellowing viruses that infected 38% of the national sugar beet crop. Controlling up to 50 % of aphids by parasitoids or other natural enemies, whilst commendable in a situation where aphids might cause significant yield loss by direct-feeding, is certainly not sufficient to prevent widespread virus infection within crops, simply because it does not take many aphids to spread viruses. The threshold for aphid control using insecticide sprays in sugar beet in the UK is, and has been for decades, one green wingless aphid per four plants. This level of infection is actually quite difficult for a human to detect to make the judgement to use a spray or not, and it is a population level that does not attract many parasitoids or predators, even though they are better at finding them than we humans. But that threshold is necessary to help reduce the spread of the yield-reducing viruses and protect the yield of sugar beet.

The Effect of Palizin, Tondexir and Pymetrozine on The Green Cotton Aphid Aphis gossypii

Moghadam, M., B. Amiri-Besheli and M. Sharifi. 2022. The Effect of Palizin, Tondexir and Pymetrozine on The Green Cotton Aphid Aphis gossypii. Arab Journal of Plant Protection, 40(4): 340-345. https://doi.org/10.22268/AJPP-40.4.340345 The aim of this study was to evaluate the effect of plant-based pesticides on the green aphid A. gossypii and also investigating the effect of adding citral oil to the plant pesticides. To carry out experiments on A. gossypii in nymph and adult stages, young soybean leaves of DPX cultivar were used (Katoul). For bioassay, first, the bulk solution was prepared and then, other concentrations (eight concentrations for each insecticide) were prepared and used. The results of this study showed that the palizin insecticide with citral oil at LC50 of 359.669 ml/l had the greatest effect on nymphs in-vitro, but when the same nymph stage was evaluated under greenhouse conditions, the highest efficacy was obtained with the mixture of pymetrozine and citral oil with LC50 of 295.242 ml/L. In the experiments performed on the adult stage under both greenhouse and laboratory conditions, the most desirable treatment was that of the mixture of herbal insecticide palizin and citral oil, and the LC50 obtained was 807.88 and 1249.14 ml/L under laboratory and greenhouse conditions, respectively. Keywords: Palizin, pymetrozine, tondexir, citral oil, cotton green aphid.

Assessment of pepper genetic resources for Verticillium wilt resistance

Study was carried out for determining the reaction of 83 peppers varieties, breeding lines, and local forms /landraces/ to fungal pathogen Verticillium dahliae Kleb. as they are the part of the Balkan Capsicum core collection which was deeply phenotyped in the recent years. The development of the disease was reported twice and high degree of resistance was reported for 41 accessions. The highest percentage of highly resistant group of genotypes was 72%, followed by resistant group with 23%. The infestation in the groups of medium sensitive, sensitive and highly sensitive was respectively 1%, 3%, and 1%. The highly sensitive (0 - 19%), sensitive (20 - 39%) and medium sensitive (40 - 59%) are consisted by the var. blocky, var. ratundum and var. kapia accessions. The group of resistant (60 - 79%) was dominated by var. ratundum and var. blocky. Materials from var. kapia prevail of highly resistant (80 - 100%). The group of pungent peppers accessions was more resistant to Verticillium infestation. On the base of current and previous results may concluded that four accessions were identified as resistant to TMV and non-infested by Verticillium while two ones possessed low infestation by green aphids, trips and cotton bollworm and non-attacked by Verticillium. Other seven genotypes combined lack of infestation by Verticillium wilt with high levels of three and more agronomic and biochemical traits.

Assessment of Non-Neonicotinoid Treatments Against Green Aphids on Sugar Beets

Assessment of Non-Neonicotinoid Treatments Against Green Aphids on Sugar Beets

Ecological Studies on the Peach Green Aphid, Myzus persicae and its Natural Enemies

Ecological Studies on the Peach Green Aphid, Myzus persicae and its Natural Enemies

The Impact of Ultraviolet-B Radiation on the Sugar Contents and Protective Enzymes in Acyrthosiphon pisum.

Simple SummaryThe decrease of stratospheric ozone contributes to a significant increase in solar ultraviolet (UV-B) radiation. This effect has led to investigation on the impact of increased UV-B radiation on insect physiology. Pea aphid is a worldwide important agricultural pest and is difficult to control due to its small size, high fecundity, and non-sexual reproduction. As such, there is a need for study of the effects of UV-B radiation on aphid physiology, to raise awareness of the mechanisms of aphid responses to UV-B stress. The results reported here revealed that UV-B radiation can lead to an increase in sugar contents in both red and green morphs of this aphid species, and confirmed the effects of UV-B radiation on aphid physiology by means of influencing protective enzyme activity.Natural and anthropogenic changes have been altering many environmental factors. These include the amount of solar radiation reaching the Earth’s surface. However, the effects of solar radiation on insect physiology have received little attention. As a pest for agriculture and horticulture, aphids are one of the most difficult pest groups to control due to their small size, high fecundity, and non-sexual reproduction. Study of the effects of UV-B radiation on aphid physiology may provide alternative control strategies in pest management. In this study, we examined the effects of UV-B radiation on protein and sugar contents, as well as the activities of protective enzymes, of the red and green morphs of the pea aphid over eight generations. The results indicated a significant interaction between UV-B radiation and aphid generations. Exposure of the pea aphids to UV-B radiation caused a significant decrease in the protein content and a significant increase in the glycogen and trehalose contents at each generation as measured in whole aphid bioassays. The enzyme activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) of the pea aphids changed significantly at each generation with UV-B treatments. The SOD activity increased over eight generations to the highest level at G7 generation. However, the enzyme activity of CAT first increased and then decreased with UV-B treatments, and POD mostly gradually decreased over the eight generations. Therefore, UV-B radiation is an environmental factor that could result in physiological changes of the pea aphid. Moreover, our study discovered that red and green aphids did not display a significant consistent difference in the response to the UV-B treatments. These results may prove useful in future studies especially for assessing their significance in the adaptation and management against UV-B radiation.

Toxicity of Varronia curassavica Jacq. Essential Oil to Two Arthropod Pests and Their Natural Enemy.

This study aimed to evaluate the toxicity of the Varronia curassavica Jacq. essential oil to two cosmopolitan and polyphagous pest species, the two-spotted spider mite (Tetranychus urticae Koch) and the green aphid (Myzus persicae Sulzer). Additionally, we tested the essential oil toxicity to a generalist predatory insect, the green lacewing Ceraeochrysa cubana Hagen. The treatments consisted of four essential oil concentrations (0.25, 0.5, 0.75 and 1.0%) and one control (Tween® 80 + water). At 0.75% concentration, the V. curassavica essential oil caused the highest mortalities for both pests. The concentration of 1.0% of the essential oil interfered mainly in the rates of oviposition and egg hatching and caused a decrease in the instantaneous population growth rate (ri) of T. urticae. Survival of the C. cubana was not affected by the highest tested concentration of the essential oil (1%). Tested concentrations V. curassavica essential oil were toxic to T. urticae and M. persicae, but not to the predator C. cubana.

The Impact of Phloem Feeding Insects on Leaf Ecophysiology Varies With Leaf Age.

Herbivore insects have strong impacts on leaf gas exchange when feeding on the plant. Leaf age also drives leaf gas exchanges but the interaction of leaf age and phloem herbivory has been largely underexplored. We investigated the amplitude and direction of herbivore impact on leaf gas exchange across a wide range of leaf age in the apple tree–apple green aphid (Aphis pomi) system. We measured the gas exchange (assimilation and transpiration rates, stomatal conductance and internal CO2 concentration) of leaves infested versus non-infested by the aphid across leaf age. For very young leaves up to 15 days-old, the gas exchange rates of infested leaves were similar to those of non-infested leaves. After few days, photosynthesis, stomatal conductance and transpiration rate increased in infested leaves up to about the age of 30 days, and gradually decreased after that age. By contrast, gas exchanges in non-infested leaves gradually decreased across leaf age such that they were always lower than in infested leaves. Aphids were observed on relatively young leaves up to 25 days and despite the positive effect on leaf photosynthesis and leaf performance, their presence negatively affected the growth rate of apple seedlings. Indeed, aphids decreased leaf dry mass, leaf surface, and leaf carbon content except in old leaves. By contrast, aphids induced an increase in leaf nitrogen content and the deviation relative to non-infested leaves increased with leaf age. Overall, the impacts of aphids at multiple levels of plant performance depend on leaf age. While aphids cause an increase in some leaf traits (gas exchanges and nitrogen content), they also depress others (plant growth rate and carbon content). The balance between those effects, as modulated by leaf age, may be the key for herbivory mitigation in plants.

Self and non-self recognition affects clonal reproduction and competition in the pea aphid.

The spatial interaction of clonal organisms is an unsolved but crucial topic in evolutionary biology. We evaluated the interactions between aphid clones using a colour mutant (yellow) and an original (green) clone. Colonies founded by two aphids of the same clone and mixed colonies, founded by a green aphid and a yellow aphid, were set up to observe population growth for 15 days. We confirmed positive competition effects, with mixed colonies increasing in size more rapidly than clonal colonies. In mixed colonies where reproduction started simultaneously, green aphids overwhelmed yellow aphids in number, and yellow aphids restrained reproduction. However, when yellow aphids started to reproduce earlier, they outnumbered the green aphids. To test whether aphids have the ability to control reproduction according to the densities of self and non-self clones, one yellow aphid or one antennae-excised yellow aphid was transferred into a highly dense green clone colony. Intact yellow aphids produced fewer nymphs in crowded green colonies, whereas the fecundity of antennae-excised aphids did not change. Thus, we conclude that aphid clones can discriminate between self and non-self clones, and can regulate their reproduction, depending on whether they are superior or inferior in number to their competitors.

Incidence of potato green aphid and Lyriomiza sp. in radish plants grown on different levels of organic fertilizer

Radish is a short cycle horticultural product from family Brassicaceae. Between the insects found on radish leaves, potato green aphid (Myzus persicae Sulzer) is one of the main pests of this culture, causing economic damage. Synthetic fertilizers used in conventional crops can promote changes in the pattern of amino acids in plants, leading to a higher insect damage. The source of fertilization is one of the factors that could influence in the incidence of pests. The objective of the study was to evaluate the incidence of potato green aphid and other pests on radish plants grown with different doses of organic fertilizer. The experiment was carried under semi-field conditions, in Fortaleza (Ceará State). A completely randomized design was used, with six replicates. The treatments consisted of five doses of organic compost (0; 45; 60; 75; 90 t.ha-1). For the population survey of pests and natural enemies, counts were made in three evaluations (7, 14 and 21 days after sowing). The counting data were subjected to deviance analysis using a generalized linear model (GLM). It was observed significant differences in the number of adults M. persicae in the treatments of 75 and 90 t.ha-1. Presence of leafminer (Lyriomiza sp.) was observed on plants without and with the lower doses of organic fertilizer. Even using organic fertilizers that promote a slower liberation of nutrients, high incidence of pests (Mainly M. persicae) was observed on radish plants.

Survey on Population Changes of Aphis gossypii (Glove.) on Cluster Cultivars in Ultra Narrow Row system in the Field Cotton Golestan Province of Iran.

In the planting system, the row spacing is very narrow, where the spacing of the planting rows is considered to be between 20 and 40 cm. In this system, the number of bolls per plant is reduced. Experiments to survey and compare populations Aphis gossypii on 3 cluster cultivars and one control, using the method of narrow planting distance of 20 × 20 and 20 × 80 cm as a factorial design in the form of a randomized complete block design with 3 replications in Hashemabad cotton research station in Gorgan during two years 2018- 2019 was evaluated. A sampling of different stages of cotton aphids was recorded weekly in the tested treatments and recorded in special tables. Based on the studies, the results of combined analysis of variance show that the yield in the cultivars tested in Sajedi cultivar with a distance of 80 cm with an average wash of 3392 kg/ha in the plot and Golestan cultivar 80 cm with 3245.8 kg/ha in the plot with the highest yield and T2 cultivar With a distance of 20 cm, 1694 kg/ha in Crete had the lowest yield. Infestation rate of cluster cultivars tested in very narrow cultivation of green aphid population density on Sajedi cultivars with a distance of 80 cm and the khorshid with a distance of 20 cm with densities of 41.39 and 40.36 aphids per leaf, respectively, with the highest aphids per leaf and cultivars T2 and the khorshid with a distance of 20 cm with 23.59 and 29.04 aphids, respectively. They had the least infestation in the leaves.

Population Changes of Important Sucking-Pests <i>Aphis gossypii</i> (Glove.) and <i>Bemisia tabaci</i> (G.) in the New Varieties Second Plant (After Canolai) of Iran

Investigating the population dynamic of important cotton pests on new cotton varieties is important. Cotton cultivars have important pests such as thrips, aphids, whitefly and bollworm. Considering the premature cultivars with proper yield for second cultivation in cotton-growing Golestan province at the north of Iran, this study seems necessary. This genotypes was cultivated with 9 treatments in 4 replicates. A completely randomized block design was carried out in Hashem Abad research station in a spray-free plot with the dimensions of 30 × 80 cm in 2018-2019 years. According to the results of this study, analysis of variance of two years showed that the yield of N2G80 with 1538 g in the plot with 41.7% lint percentage had the highest yield, and M13 with 685 g in the plot and 35.8% lint percentage in the lowest yield they had. The infestation rate of the genotypes tested in the population of green aphid of cotton in K880-2 and Golestan® cultivars was 48.88 and 40.31 in aphid with the highest density, and the SKT-133 and TBL-60 genotype respectively with 27.15 and 29.11 aphids per leaf had the least infestation. The amount of infestation of the genotypes tested to the population of whitefly cotton on the N2G80 and TBL-60 genotype was 90.76 and 68.86 whitefly in the leaf with the highest infestation and N2G80 and TBL-60 genotype respectively with 40.05 and 75.51 white fly in leaf were the least infested. Correlation coefficient between traits was equal to 0.55. The number of bolls per plant was not correlated with other traits including boll weight, lint percentage, aphid and white fly.