Greater Dry Matter Research Articles

Effects of dietary black cumin (Nigella sativa L.) meal on performance, blood metabolites, and digestibility in a rice straw-based diet of fattening Garut lambs.

During black cumin oil production, black cumin meal (BCM) is produced as a by-product. This study investigated the potential use of BCM to partly replace concentrate in a rice straw-based diet of fattening Garut lambs. Twenty-eight heads of male Garut lambs aged approximately10 months with an average initial body weight of 20.7 kg/head (coefficient of variation 12.9%) were used. A completely randomized design was used to compare four different levels (0% [control], 5% [BCM-5], 10% [BCM-10], and 15% [BCM-15]) of BCM in rice straw- and concentrate-based diets on the performance of Garut lambs during 35- and 70-day feeding trials using seven replicates (n = 7). Blood metabolites and nutrient digestibility were also measured after the performance study. BCM was rich in crude protein (36.8%) and tannins (21.6%). The BCM-10 and BCM-15 treatments increased (p < 0.05) average daily gain and feed efficiency compared with the control treatment in the 35-day feeding trial. All BCM treatments had greater dry matter (DM) intake compared with the Control at 70 days (p < 0.05). Furthermore, BCM-10 and BCM-15 had greater (p < 0.05) total protein, DM, and organic matter (OM) digestibility. BCM-15 had the highest (p < 0.01) blood triglyceride while BCM-10 tended to have higher (p < 0.1) blood glucose concentrations among the other treatments. BCM supplementation can partly replace concentrate and improve the overall quality of rice straw and concentrate-based diets, resulting in improved performance of fattening Garut lambs due to increased DM and OM digestibility, as well as protein and energy absorption. Approximately 10% of the BCM supplementation is suggested as the optimum level.

Effects of tillage and maturity stage on the yield, nutritive composition, and silage fermentation quality of whole-crop wheat.

Whole-crop wheat (Triticum aestivum, WCW) has a high nutritional value and digestibility. No-tillage (NT) can reduces energy and labor inputs in the agricultural production process, thus decreasing production costs. There are many studies on planting techniques of WCW at present, few being on no-tillage planting. This study aimed to compare the effects of different tillage methods and maturity stages on the yield, nutritive value, and silage fermentation quality of WCW. The experiment included two tillage methods (NT; conventional tillage, CT), two maturity stages (flowering stage; milk stage), and three years (2016-2017; 2017-2018; 2018-2019). Years had a strong influence on the yield and nutritional composition of WCW. This was mainly related to the amount of rainfall, as it affects the seedling emergence rate of wheat. Although tillage methods showed no significant effects on the yield, plant height, and stem number per plant of WCW (P > 0.05), compared to CT, the dry matter (DM) and crude protein (CP) yields of NT decreased by 0.74 t/ha and 0.13 t/ha. Tillage methods showed no significant effects on the nutritive composition of WCW (P > 0.05). The WCW at the milk stage had greater DM (5.25 t/ha) and CP (0.60 t/ha) yields than that at the flowering stage (3.19 t/ha and 0.39 t/ha) (P< 0.05). The acid detergent fiber concentration of WCW decreased by 34.5% from the flowering to the milk stage, whereas water-soluble carbohydrates concentration increased by 50.6%. The CP concentration at the milk stage was lower than that at the flowering stage (P< 0.05). The lactic acid concentration of NT (17.1 g/kg DM) silage was lower than that of CT (26.6 g/kg DM) silage (P< 0.05). The WCW silage at the milk stage had a lower NH3-N concentration (125 g/kg TN) than that at the flowering stage (169 g/kg TN) (P< 0.05). Wheat sown by NT and CT was of similar yield and nutritional value, irrespective of harvest stages. WCW harvested at the milk stage had greater yield and better nutritional composition and silage fermentation quality than that at the flowering stage. Based upon the results of the membership function analysis, no-tillage sowing of wheat was feasible and harvesting at milk stage was recommended.

Effects of feeding hybrid rye grain as a replacement for barley grain on dry matter intake, ruminal fermentation, and the site and extent of nutrient digestion in finishing beef heifers.

The objective of this study was to evaluate effects of increasing the inclusion of dry-rolled hybrid rye (HR) as a replacement for dry-rolled barley grain (DRB) on feed intake, ruminal fermentation, and the site and extent of nutrient digestion for finishing cattle. Eight ruminally and duodenally cannulated Hereford-cross heifers were used in a replicated 4 × 4 Latin square design with 21-d periods including 15 d of dietary adaptation and 6 d of data and sample collection. Dietary treatments included a control diet with 10.00% grass hay, 85.21% DRB, 4.51% of a vitamin and mineral supplement, and 0.28% of urea on a dry matter (DM) basis. Hybrid rye grain replaced 33%, 67%, or 100% of the DRB. Feed ingredients, feed refusals, ruminal pH, ruminal fluid, duodenal digesta, and fecal samples were collected from days 18 to 21 in each period. Data were analyzed using the Proc Glimmix procedure of SAS 9.4 (SAS Inst. Inc., Cary, NC) to evaluate the linear, quadratic, and cubic effects of increasing HR inclusion. Increasing HR inclusion as a substitute for DRB linearly decreased (P < 0.01) DM intake, linearly decreased mean ruminal pH (P < 0.01), and increased the duration (P < 0.01) and area (P = 0.02) that ruminal pH was < 5.5. There were no effects of HR inclusion on total short chain fatty acid and lactic acid concentrations in ruminal fluid. Likewise, the molar proportions of acetate and butyrate were not affected by HR inclusion. Propionate was cubically affected by HR inclusion (P = 0.02). Ruminal ash-free neutral detergent fiber (aNDFom) digestibility linearly increased (P = 0.03) with increasing HR, but there was no effect on ruminal starch digestibility averaging 71.1% (SEM = 3.611). Increasing HR inclusion linearly increased intestinal DM digestibility (% of flow to the duodenum; P = 0.03), tended to linearly increase intestinal digestibility of organic matter (P = 0.08), and tended to quadratically affect intestinal digestibility of aNDFom (P = 0.07). Increasing hybrid rye linearly increased apparent total tract DM, organic matter, crude protein, aNDFom, and starch digestibility (P ≤ 0.05). In addition, increasing HR inclusion linearly increased GE digestibility (P < 0.01) and the DE concentration (P < 0.01). Increasing the inclusion rate of HR grain as a substitute for DRB in finishing diets decreased DMI and increased risk for low ruminal pH, which may be influenced by greater digestible energy concentration arising from greater DM, OM, aNDFom, and starch digestibility.

Forage productivity and morphogenesis of Trachypogon vestitus as affected by phosphate fertilization levels in the Roraima’s savannas

The effects of phosphate fertilization (0, 40, 80 and 120 kg of P2O5 ha-1) on forage production and morphogenetic and structural characteristics of Trachypogon vestitus pastures were evaluated under natural field conditions in the savannas of Roraima. Phosphate fertilization positively and significantly (P&lt;0.05) affected green dry matter (GDM) production, absolute growth rate (AGR), number of tillers plant-1, number of leaves tiller-1 (NLT), average leaf size (ALS), leaf tiller area (LTA), appearance rates (LAR), expansion (LER) and leaf senescence rates. The maximum yields of GDM, AGR, LAR, LER, NLT, LTA and ALS were obtained with the application of 109.2; 106.4; 56.1; 81.5; 97.4; 58.6 and 63.7 kg of P2O5 ha-1, respectively. The efficiency of P utilization was inversely proportional to the P doses applied.

Phosphate fertilization of Mesosetum chaseae in Roraima’s savannas

The objective of this work was to evaluate the effect of phosphate fertilization (0, 30, 60 and 120 kg of P2O5 ha-1) on forage production and morphogenetic and structural characteristics of Mesosetum chaseae under natural field conditions in the savannas of Roraima. Phosphate fertilization positively and significantly (P&lt;0.05) affected green dry matter (GDM) production, absolute growth rate (AGR), number of tillers plant-1, number of leaves tiller-1 (NLT), average leaf size (ALS), leaf tiller area (LTA), appearance rates (LAR), expansion (LER) and leaf senescence rates. The maximum yields of GDM, AGR, LAR, LER, NLT, LTA and ALS were obtained with the application of 89.17; 112.7; 47.7; 51.9; 102.9; 101.8 and 94.8 kg of P2O5 ha-1, respectively. The efficiency of P utilization was inversely proportional to the P doses applied.

Agronomic performance and morphogenic and structural characteristics of Axonopus aureus pastures under phosphate fertilization levels in the Roraima’s savannas

The effect of phosphate fertilization (0, 30, 60 and 120 kg of P2O5 ha-1) on forage production and morphogenetic and structural characteristics of Axonopus aureus was evaluated under natural field conditions in the savannas of Roraima. Phosphate fertilization positively and significantly (P&lt;0.05) affected green dry matter (GDM) production, absolute growth rate (AGR), number of tillers plant-1, number of leaves tiller-1 (NLT), average leaf size (ALS), leaf tiller area (LTA), appearance rates (LAR), expansion (LER) and leaf senescence rates. The maximum yields of GDM, AGR, LAR, LER, NLT, LTA and ALS were obtained with the application of 110.4; 108.5; 54.1; 83.5; 96.6; 59.8 and 90.6 kg of P2O5 ha-1, respectively. The efficiency of P utilization was inversely proportional to the P doses applied.

Effect of physically effective neutral detergent fiber and undigested neutral detergent fiber on eating behavior, ruminal fermentation and motility, barrier function, blood metabolites, and total tract digestibility in finishing cattle.

This study evaluated the effects of physically effective neutral detergent fiber (peNDF) and undigested neutral detergent fiber (uNDF) on eating behavior, ruminal fermentation and motility, barrier function, blood metabolites, and total tract nutrient digestibility for finishing cattle. Six Simmental heifers (668 ± 28.4 kg BW) were used in a replicated 3 × 3 Latin square (21 d periods) balanced for carry-over effects. Treatments included a control (CON; Table 1) with no forage peNDF and minimal uNDF (peNDF: 0.0%, and uNDF: 4.88 ± 0.01; 95.15% barley grain, 4.51% vitamin and mineral supplement, and 0.34% urea on a DM basis). Pelleted wheat straw (PELL) was included at 10% of dietary DM by replacing barley grain to provide added uNDF but no forage peNDF (peNDF: 0.00%, and uNDF: 6.78 ± 0.02%). Finally, chopped wheat straw (STR) was included as a replacement for pelleted wheat straw to provide forage peNDF and uNDF (peNDF: 1.74 ± 0.06%, and uNDF: 6.86 ± 0.03%). Dry matter intake was not affected (P = 0.93) by treatments. Cattle fed CON spent less time ruminating (P = 0.010) and had less meals/d (P = 0.035) when compared with cattle fed STR, with those fed PELL being intermediate but not different from other treatments. Cattle fed CON had lesser ruminal pH (P = 0.020), and a greater duration that pH was < 5.5 (P = 0.020) as compared to cattle fed STR, with those fed PELL being intermediate but not different. Cattle fed CON and PELL had greater total short-chain fatty acid concentration (P = 0.003) and molar proportion of propionate (P < 0.001) when compared with cattle fed STR. Cattle fed STR had greater (P = 0.010) total ruminal pool size when compared with cattle fed CON and PELL. Cattle fed CON had greater (P = 0.043) duration between ruminal contractions when compared with cattle fed STR, with those fed PELL being intermediate. Cattle fed CON had greater serum amyloid A (P = 0.003) and haptoglobin (P < 0.001) concentration when compared with the other treatments. Cattle fed CON had greater dry matter (P < 0.001) digestibility when compared with the other treatments. In conclusion, inclusion of PELL and STR impacted eating behavior, but only STR affected ruminal fermentation, ruminal motility, systemic inflammation, and total tract nutrient digestibility in finishing cattle. These results are interpreted to suggest that the combination of peNDF and uNDF may be better than uNDF alone to adequately capture biological effects of NDF in high-concentrate diets.

Commercial Hard Squash Cultivars Exhibit Differences in Susceptibility to Phytophthora Crown Rot

Crown rot and fruit rot, caused by the oomycete Phytophthora capsici, limit the yield and quality of hard squash grown for processing. In a 2-year field study, we compared four Butternut types ( Cucurbita moschata), two Delicious varietal Hubbard types ( Cucurbita maxima), and six Kabocha types ( C. maxima) for crown rot resistance and fruit characteristics relevant to processing including mesocarp soluble solids, percentage of dry matter, and average fruit weight in 2019 and 2020. To evaluate crown rot, plants were inoculated in replicated field trials. The C. moschata cultivars Dickinson, Buckskin, New England Cheddar, and Ultra Butternut had significantly less plant death in both years (&lt;15%) than other cultivars. Significantly fewer Thunder plants died in 2020 (59.6%) compared with the Kabocha cultivars Sweet Mama (&gt;94.2%), Delica (≥92.3%), and Sunshine (≥90.4%) and had a lower relative area under the disease progress curve value (≤22.4) in both years. In noninoculated field trials, mature fruits were assessed for fruit characteristics. Of the resistant C. moschata cultivars, only Ultra Butternut exhibited similar °Brix to NK 580 in both years and had comparable or greater dry matter and fruit weight. Kabocha cultivars with moderate crown rot susceptibility (i.e., Thunder) exhibited higher °Brix and dry matter and smaller fruit weight compared with NK 580 each year. Integrated management programs can be advanced by selecting cultivars with crown rot resistance. Further evaluation of desirable yield and quality characteristics of the resistant cultivars could accelerate acceptance of these cultivars by growers and processors.

Forage productivity and morphogenesis of Mesosetum chaseae under defoliation frequency

The effect of defoliation frequency (14, 21, 28, 35, 42, 49 and 56 days) on forage production and morphogenic and structural characteristics of Mesosetum chaseae (Luces) was evaluated under natural field conditions during the dry season, in the savannas of Roraima. The reduction in pasture defoliation frequency resulted in higher green dry matter (GDM) yields, number of tillers plant-1, number of leaves tiller-1, tiller leaf area, leaf senescence rate and average leaf size. The relationship between defoliation frequency and GDM production was quadratic, with the maximum value estimated at 53.8 days of regrowth. Absolute rates of growth and leaf appearance and expansion were inversely proportional to defoliation frequencies. The highest rate of leaf expansion was obtained at 49.7 days of regrowth. Aiming to reconcile forage productivity with the optimization of the morphogenic and structural characteristics of the grass, the most appropriate period of use of its pastures is between 42 and 49 days of regrowth.

Ruminal kinetics and nutritive value of Zuri grass silage harvested at different ages and added with powder molasses.

This study aimed to evaluate the effect of regrowth ages and the use of powdered molasses on nutritional characteristics of Zuri grass (Megathyrsus maximus) silages. A completely randomized design was used, in a 3 × 3 factorial scheme: three regrowth ages (65, 80, and 95days) and three inclusion levels of powdered molasses of sugarcane (0, 20, and 40g), with four replications. The chemical composition, fermentation parameters, in vitro digestibility, and in vitro degradation kinetics of the silages were evaluated. Greater dry matter (DM) contents were observed in silages with 95days with 40g of molasses. The 65days of regrowth silages with 40g of molasses had greater in vitro digestibility of dry matter compared to other treatments. However, lower pH values, N-NH3, DM losses, gas losses, and density were observed in silages with 95days of regrowth with 40g of molasses. It is recommended to cut the Zuri grass in the range of 80 to 95days of regrowth with the addition of 40g/kg of molasses in powders for the production of silages.

Agronomic performance and chemical composition of genotypes and cultivars of Megathyrsus maximus in Roraima’s savannas

The agronomic performance of 23 genotypes and six commercial cultivars of Megathyrsus maximus was evaluated under natural field conditions at the Roraima´s savannas, during the period of June 2015 to September 2019. The evaluated parameters were green dry matter (GDM) yield, leaf, stem and dead DM yields, crude protein (CP) contents, concentrations of neutral detergent fiber and acid detergent fiber. The genotypes and cultivars evaluated affected the yields and the chemical composition of the forage. The most promising genotypes showed superior agronomic performance than the commercial cultivars evaluated. The genotypes B16; PM10; PM40 and PM14 showed the highest green dry matter yields and forage with better nutritional quality, higher CP and lower fiber contents. These showed average yields of 5,103 and 3,925 kg ha-1, for green DM and leaf DM, respectively, which represented increments of 20.7 and 12.8%, compared with those registered for the commercial cultivars. The genotype PM15 evidenced the highest productive performance during the dry season, constituting an option for regions with climatic restrictions, notably reduced rainfall. All genotypes and cultivars showed seasonal growth. The genotypes that showed the best seasonal distribution of forage production were PM11, PM36, PM30 and PM33, which provided 47.9; 46.8; 41.0 and 40.7% of GDM production during the dry season. In general, higher crude protein and lower fiber contents were directly correlated with the percentage of leaves in the available forage. The evaluated genotypes presented great genetic variability for the accumulation of forage and its chemical composition that can subsidize the generation of new commercial cultivars.

Forage productivity and morphogenesis of Mesosetum chaseae under potassium fertilization

The effect of potassium levels (0, 50, 100 and 200 kg of K2O ha-1) on green dry matter (GDM) yield and morphogenetic and structural characteristics of Mesosetum chaseae (Luces) was evaluated under natural field conditions. Potassium fertilization increased significantly (P&lt;0.05) GDM yields, absolute growth rate (AGR), number of tillers/plant, number of leaves plant-1, medium blade length, leaf area, leaf senescence rate, leaf appearance and elongation rates. Maximum GDM yields, AGR, number of tillers/plant, number of leaves/tiller, leaf area and medium blade length were obtained with the application of 167.8; 156.2; 82.7; 101.6; 101.2 and 77.5 kg of K2O ha-1, respectively. The potassium efficiency utilization was inversely proportional to the increased potassium levels.

Genetic Divergence and Spatial Configuration Influence the Weed Spectrum, Herbage Yield and Nutritive Quality of Temperate Cowpea

Under a changing climate, the biologically viable management of weeds and the exploration of the genetic divergence of spreading and towering cultivars of forage cowpea in different row configuration systems hold the potential to boost sustainable feed supply for dairy animals. A field study was undertaken to sort out the most nutritive and high-biomass-producing cultivar (Cowpea−,2007 and Rawan−,2010) of cowpea and optimize the row configuration (R × R of 15, 30, 45 and 60 cm) to manage the weed spectrum. The results revealed that Rawan-2010 remained superior in the 15 cm row configuration by recording 39% lesser weed density (WD) than the corresponding value recorded by the same cultivar sown in the 60 cm row configuration. The same treatment combination recorded a 20% lesser fresh weed weight than Cowpea−,2007 sown in the same row configuration, while it exhibited a 5.6 g m−2 lesser corresponding value of dry weed weight. In contrast, Cowpea-2010 sown in the 45 cm row configuration recorded the maximum yield attributes (stem girth, leaf and branch numbers, leaf area, fresh and dry weights per plant), except plant height (PH), which resulted in 7% and 13% higher green herbage yield (GH) and dry matter biomass (DM), respectively, than the same cultivar sown in the 30 cm row configuration. Pertaining to nutritional value, Rawan-2010 in the 45 cm row configuration yielded the maximum crude protein and minimum crude fiber content, while the same cultivar gave the greatest ash content in the wider row spacing. With GH, the correlation analyses indicated an antagonistic association for PH, a moderately linear relationship between stem girth and branch numbers and a strong direct association between leaf area and fresh plant weight.

Comparisons of Corn Stover Silages after Fresh- or Ripe-Corn Harvested: Effects on Digestibility and Rumen Fermentation in Growing Beef Cattle.

Simple SummaryCorn stover is an important agricultural byproduct, and represents an animal feedstuff. Waxy corn is harvested at milk stage or dough stage (20 d difference in harvest time) to meet the market demand for fresh corn and ripe corn in China, resulting in plenty of byproducts of corn stover after fresh- (CF) or ripe-corn (CR) harvested. This study was conducted to investigate the digestibility and rumen fermentation of these two corn stover silages in growing beef cattle. We observed that CR silage had greater dry matter and fiber contents, but with similar substrate degradation through in vitro ruminal fermentation in comparison with the CF silage. Further feeding trials indicated that feeding a CR diet (i.e., replaced 50% forage of CF silage with CR silage on a dry matter basis) increased feed intake and decreased fiber digestibility with reduction in the abundance of ruminal fiber degraders, together with similar growth performance in beef cattle, when comparing with CF diet.Both waxy corn stover after fresh- (CF) and ripe-corn (CR) harvested are important byproducts of corn cropping system and have 20 d difference in harvest time. The study aimed to investigate the effects of prolonging harvest time on the nutritive value of corn stover silage by comparing CF with CR silages. In vitro ruminal experiment was firstly performed to investigate substrate degradation and fermentation of CF and CR silages. The CR diet was formulated by replacing 50% forage of CF silage with CR silage on a dry matter (DM) basis. Fourteen crossbred steers (Simmental × Limousin × local Chinese) aged 13 months with an average weight of 318.1 ± 37.1 kg were selected and randomly allocated into two dietary treatment groups. Although the CR silage had greater DM and fiber contents than CF silage, it did not alter in vitro degradation (p > 0.05), but with lower molar percentage of propionate and acetate to propionate ratio (p < 0.05). The cattle fed CR diet had a higher DM intake and lower fiber digestibility with reduction in 18S rRNA gene copies of protozoa and fungi and 16S rRNA gene copies of Fibrobacter succinogenes (p < 0.05). Further 16S rRNA gene amplicon analysis indicated a similar diversity of bacteria community between CR and CF treatments (p > 0.05). Few differences were observed in the abundance of genera larger than 1% (p > 0.05), except for the reduction in abundance of genera Ruminococcaceae_NK4A214_group in CR treatment (p < 0.05). In summary, prolonging 20 d harvest time of corn stover silage increases the forage fiber and DM content, which promotes feed intake with decreased fiber degradation, although rumen fermentation and growth performance are not changed in growing beef cattle.

In natura residues from peach palm heart industry for ruminant feed

Palm heart processing generates a large amount of residues like leaves, sheath and stems that have potential for ruminant feeding. This study aimed to evaluate the effect of in natura peach palm heart (Bactris gasipaes Khunt.) residues on performance, dry matter (DM) digestibility and intake of ruminants. External sheath was the most suitable residue for sheep, cattle and buffaloes feeding, although sheep presented rejection of 43.5 g kg-1. Thereby, assays of apparent and in vitro dry matter digestibility were done under four feeding treatments, which were the exclusive peach palm sheath, and that one mixed with residues of banana and rice, besides citrus pulp. The experiment was carried out under randomized complete design, with five replications. Greater DM intake (p &lt; 0.05) were observed in animals fed with peach palm sheath mixed with rice (1.12 kg day-1) and mixed with citrus pulp (0.91 kg day-1), however there were no difference among treatments regarding the sheep final weight (p &gt; 0.05). Cattle and buffaloes accept different types of peach palm residues, unlike sheep that present a low rejecting for them. Citrus pulp and rice residue raise the roughage quality. Peach palm residues can be an alternative roughage source to feed ruminants.

140 Effects of Calcium Carbonate Level on Weanling Pig Growth Performance and Fecal Dry Matter

Abstract A total of 695 barrows (DNA 200×400; initially 5.9 ± 0.02 kg) were used in 2 groups in a 28-d study to evaluate the effects of dietary calcium carbonate level on nursery pig growth performance and fecal dry matter. Upon arrival to the research facility, pigs were randomly assigned to pens with 5 pigs/pen, and pens were allotted to 1 of 5 dietary treatments with 27 or 28 pens/treatment. Dietary treatments were formulated to provide 0, 0.45, 0.90, 1.35, and 1.80% calcium carbonate added at the expense of corn, which resulted in total Ca of 0.49, 0.66, 0.84, 1.01 and 1.18%, respectively. All diets were formulated to contain 0.68% total P and 0.59% STTD P. Diets were fed in 2 phases with treatment diets fed from d 0 to 14 and a common diet fed from d 14 to 28. Treatment diets were fed in meal (group 1) and pellet (group 2) form. Feces were collected from 3 pigs/pen and dried to calculate fecal dry matter. There was no evidence for a treatment×group interaction and thus, groups were combined. From d 0 to 14 (treatment period), ADG (P = 0.010), d 14 BW (P = 0.006), and G:F (P = 0.010) decreased linearly as calcium carbonate increased. There was no evidence for a difference in ADFI (P &amp;gt; 0.10) between treatments. For fecal dry matter, there was a tendency for a quadratic response (P = 0.091) with the highest and lowest calcium carbonate diets having the greatest dry matter. Subsequent performance from d 14 to 28 was not influenced by previous treatment (P &amp;gt; 0.10). In summary, increasing dietary calcium carbonate from 0 to 1.80% decreased ADG and G:F in pigs the first 14-d after weaning.

Dietary inclusion of ruminally protected linseed oil as a means to mitigate heat and slaughter-induced stress in feedlot cattle.

There is evidence of a relationship between increased energy intake and the development of metabolic inflammation and insulin resistance (IR), and between the aforementioned metabolic state and impaired tolerance to heat stress. Based on the anti-inflammatory properties and mitigating effects on IR and stress of n-3 polyunsaturated fatty acids (n-3 PUFA), an experiment was performed to evaluate the effect of n-3 PUFA supplementation to feedlot-finished steers during summer on animal performance, physiological and biochemical variables associated with glucose metabolism, heat and preslaughter-induced stress, and meat quality. A total of 48 Angus steers (388±2kg) were fed one of three corn-based finishing diets containing (dry matter basis) 0% added oil (CON; negative control), or 1.90% of sunflower oil-calcium salt (SUN; positive control), or 1.92% of linseed oil-calcium salt (LIN). There was a trend (P = 0.08) for greater dry matter intake (DMI) and greater (P = 0.02) average daily gain (ADG) in LIN-fed animals compared with the average between those that received the CON or SUN diets, whereas no differences (P ≥ 0.34) were observed between the latter. No other performance, physiological, or carcass variables were affected (P ≥ 0.12) by treatment. Blood glucose and insulin were similar (P ≥ 0.14), though the homeostatic model assessment (HOMA) which gauges IR tended (P = 0.06) to be reduced for LIN-fed animals compared with the average between those that received the CON or SUN diets. Blood insulin and HOMA increased linearly (P ≤ 0.01) with days on feed. An interaction between the study phase (feeding period or slaughter) and treatment was observed (P ≤ 0.05) for glucose and cortisol. While the magnitude of glucose increase (P < 0.01) from the end of the feeding period to slaughter was greater for CON- and SUN-fed animals compared with LIN-fed ones, cortisol increased (P < 0.05) only in animals that received CON or SUN diets. Meat quality attributes were not affected (P ≥ 0.16) by treatment. The concentration of n-3 PUFA was greater (P < 0.01) and n-6:n-3 ratio was lesser (P < 0.01) in meat from LIN-fed animals compared with that resulting from the average between the animals that received the negative (CON) or positive (SUN) control diets. Results suggest that n-3 PUFA supplementation mitigated metabolic alterations associated with IR and preslaughter-related stress. It may have also improved tolerance to heat, resulting in greater DMI and ADG of steers fed a high-energy diet during summer. Results also indicate that glucose metabolism and heat stress tolerance worsen with time when feeding concentrate-based diets.

Demanda nutricional e exportação de nutrientes por cultivares modernas de feijão-comum

Abstract The objective of this work was to quantify the extraction and export of nutrients by common bean (Phaseolus vulgaris) cultivars from different commercial groups and with different growth habits. The experiment was conducted using four common bean cultivars: TAA Gol, BRS FC104, IPR Tuiuiú, and TAA Dama. Total dry matter (DM) and nutrient accumulation in the V4 (with four, six, and eight three-leaflet leaves), R5, R7, R8, and R9 stages was determined, as well as grain yield and nutrient export. Differences were observed both in nutrient uptake and in DM and nutrient accumulation among the cultivars in the initial stages of development (V4 with six and eight three-leaflet leaves). The TAA Gol cultivar showed a greater accumulation of almost all nutrients in these two initial stages. In R7, the cultivar that had the greatest DM and nutrient accumulation was TAA Dama, but, from R8 onwards, there were no significant differences. Although nutrient export is similar among the evaluated cultivars, a lower percentage is translocated to the grain in TAA Dama.

Yield, zinc efficiencies and biofortification of wheat with zinc sulfate application in soil and foliar nanozinc fertilisation

Context Agronomic biofortification of wheat (Triticum aestivum L.) with zinc (Zn) is an effective approach to increase grain Zn concentration and productivity and alleviate Zn malnutrition in humans. Foliar Zn application is an alternative strategy to endorse soil Zn deficiency with better grain Zn partitioning. Aims This study aimed to better understand dose management of soil and foliar Zn application in wheat for biofortification. Methods The objectives was to evaluate the effect of foliar applied nano Zn doses (0, 0.75, 1.5, 3 and 6 kg/ha (zinc oxide, ZnO) 50% at tillering and 50% at grain filling in combination) with soil Zn application (0 and 8 kg/ha, as zinc sulfate) on growth, nutrition, Zn use efficiencies, intake and yield biofortification of wheat in 2019 and 2020 under Brazilian savanna. Key results Combined foliar and soil Zn application increased shoot and grains Zn concentration and accumulation with greater dry matter (9.8 and 10.6%) and grain yield (9.8 and 11%) of wheat as compared to control in 2019 and 2020 respectively. Zinc use efficiency (ZnUE), Zn utilisation efficiency and applied Zn recovery improved with soil Zn application and 2.5 kg/ha foliar nano Zn, but decreased with further increase in foliar Zn application. Zn sulfate stood out for increasing crop productivity while foliar spray with nano Zn for better grains biofortification of wheat. Conclusions Soil Zn application along with 3 kg/ha of foliar nano Zn increased plant and grains Zn concentration and accumulation, dry matter, grain yield, Zn partitioning index and Zn intake in wheat in tropical conditions of Brazil. Implications The combined application of soil and foliar Zn in harsh tropical savannah condition could better improve Zn nutrition, crop growth, and productivity with better Zn biofortification and intake of wheat.

Feeding rumen-protected lysine to dairy cows prepartum improves performance and health of their calves

Providing adequate concentrations of AA in the prepartum diet is pivotal for the cow's health and performance. However, less is known about the potential in utero effects of particular AA on early-life performance of calves. This experiment was conducted to determine the effects on dairy calves when their dams were fed rumen-protected lysine (RPL; AjiPro-L Generation 3, Ajinomoto Heartland Inc.; 0.54% dry matter of total mixed ration as top dress) from 26 ± 4.6 d (mean ± standard deviation) before calving until calving. Seventy-eight male (M) and female (F) Holstein calves were assigned to 2 treatments based on their dams' prepartum treatment, RPL supplementation (PRE-L) or without RPL (CON). At the time of birth (0.5-2 h after calving), before colostrum was fed, blood samples were collected. An initial body weight was obtained at 1 to 3 h after birth. Calves were fed 470 g of colostrum replacer (Land O'Lakes Bovine IgG Colostrum Replacer, Land O'Lakes, Inc.) diluted in 3.8 L of water. Calves were provided water ad libitum and fed milk replacer (Advance Excelerate, Milk Specialties Global Animal Nutrition; 28.5% crude protein, 15% fat) at 0600 h and 1700 h until 42 d of age. Calves were measured weekly, at weaning (d 42), and at the end of the experimental period (d 56). Plasma concentrations of AA were measured on d 0, 7, and 14 d using ultra-performance liquid chromatography-mass spectrometry (Waters) with a derivatization method (AccQ-Tag Derivatization). Final body weight was greater for M (87 ± 11 kg) than F (79 ± 7 kg). Calves in PRE-L tended to have greater dry matter (814 ± 3 g/d) and crude protein (234 ± 6 g/d) intakes than those in CON (793 ± 9 g/d and 228 ± 11 g/d, respectively). Calves in PRE-L had greater average daily gain (0.96 ± 0.04 kg/d) than calves in CON (0.85 ± 0.03 kg/d) during wk 6 to 8. Calves in PRE-L tended to be medicated fewer days than CON (4.7 ± 1.2 d vs. 6.2 ± 3.4 d, respectively). Calves in PRE-L-M and CON-F (2,916 ± 112 µM and 2,848 ± 112 µM, respectively) had greater total AA concentration in plasma than calves in PRE-L-F and CON-M (2,684 ± 112 µM and 2,582 ± 112 µM, respectively). Calves in PRE-L-F and CON-M (4.09 ± 0.11% and 4.16 ± 0.11%, respectively) had greater concentration of Lys as a percentage of total AA compared with calves in CON-F and PRE-L-M (3.91 ± 0.11% and 3.90 ± 0.11%, respectively). Calves in PRE-L tended to have greater percentage of phagocytic neutrophils (39.6 ± 1.59%) than calves in CON (35.9 ± 1.59%). In conclusion, increasing the metabolizable lysine provided to prepartum dairy cows had modest effect over offspring performance, with the major result being a greater average daily gain for calves in PRE-L during the preweaning phase (wk 6-8).