Gray Level Co-occurrence Matrix Texture Research Articles

Accurate Estimation of Gross Primary Production of Paddy Rice Cropland with UAV Imagery-Driven Leaf Biochemical Model

Accurate estimation of gross primary production (GPP) of paddy rice fields is essential for understanding cropland carbon cycles, yet remains challenging due to spatial heterogeneity. In this study, we integrated high-resolution unmanned aerial vehicle (UAV) imagery into a leaf biochemical properties-based model for improving GPP estimation. The key parameter, maximum carboxylation rate at the top of the canopy (Vcmax,025), was quantified using various spatial information representation methods, including mean (μref) and standard deviation (σref) of reflectance, gray-level co-occurrence matrix (GLCM)-based features, local binary pattern histogram (LBPH), and convolutional neural networks (CNNs). Our models were evaluated using a two-year eddy covariance (EC) system and UAV measurements. The result shows that incorporating spatial information can vastly improve the accuracy of Vcmax,025 and GPP estimation. CNN methods achieved the best Vcmax,025 estimation, with an R of 0.94, an RMSE of 19.44 μmol m−2 s−1, and an MdAPE of 11%, and further produced highly accurate GPP estimates, with an R of 0.92, an RMSE of 6.5 μmol m−2 s−1, and an MdAPE of 23%. The μref-GLCM texture features and μref-LBPH joint-driven models also gave promising results. However, σref contributed less to Vcmax,025 estimation. The Shapley value analysis revealed that the contribution of input features varied considerably across different models. The CNN model focused on nir and red-edge bands and paid much attention to the subregion with high spatial heterogeneity. The μref-LBPH joint-driven model mainly prioritized reflectance information. The μref-GLCM-based features joint-driven model emphasized the role of GLCM texture indices. As the first study to leverage the spatial information from high-resolution UAV imagery for GPP estimation, our work underscores the critical role of spatial information and provides new insight into monitoring the carbon cycle.

A Hybrid Segmentation Algorithm for Rheumatoid Arthritis Diagnosis Using X-ray Images

Rheumatoid Arthritis (RA) is a chronic autoimmune illness that occurs in the joints, resulting in inflammation, pain, and stiffness. X-ray examination is one of the most common diagnostic procedures for RA, but manual X-ray image analysis has limitations because it is a time-consuming procedure and is prone to errors. A specific algorithm aims to a lay stable and accurate segmenting of carpal bones from hand bone images, which is vitally important for identifying rheumatoid arthritis. The algorithm demonstrates several stages, starting with Carpal bone Region of Interest (CROI) specification, dynamic thresholding, and Gray Level Co-occurrence Matrix (GLCM) application for texture analysis. To get the clear edges of the image, the component is first converted to the greyscale function and thresholding is carried out to separate the hand from the background. The pad region is identified to obtain the contours of it, and the CROI is defined by the bounding box of the largest contour. The threshold value used in the CROI method is given a dynamic feature that can separate the carpal bones from the surrounding tissue. Then the GLCM texture analysis is carried out, calculating the number of pixel neighbors, with the specific intensity and neighbor relations of the pixels. The resulting feature matrix is then employed to extract features such as contrast and energy, which are later used to categorize the images of the affected carpal bone into inflamed and normal. The proposed technique is tested on a rheumatoid arthritis image dataset, and the results show its contribution to diagnosis of the disease. The algorithm efficiently divides carpal bones and extracts the signature parameters that are critical for correct classification of the inflammation in the cartilage images.

Texture feature-aware consistency for semi-supervised honeycomb lung lesion segmentation

Accurate segmentation of honeycomb lung lesions from lung CT images is crucial for the clinical diagnosis of a wide range of lung diseases. Existing semi-supervised segmentation methods ignore the rich texture feature information in honeycomb lung CT images, resulting in inaccurate predictions in the lesion region. Therefore, this paper proposes a semi-supervised honeycomb lung lesion segmentation framework using texture feature-aware consistency. The framework consists of a shared encoder, a main decoder, and three auxiliary decoders. The different outputs produced by the decoders are used to compute the Grey Level Co-occurrence Matrix and extract texture features, respectively, and further to compute the loss of texture feature-aware consistency. In pseudo-labeling supervision, the uncertainty of each output is computed to bootstrap the pseudo-labeling supervision loss. For the small lesion recognition omission problem, a pseudo-label refinement module is constructed to reuse the multi-scale features of the encoder, focusing on small-scale lesions, and refining the pseudo-labels. Experimental results show that the proposed method outperforms other methods in the honeycomb lung segmentation task, achieving a Dice coefficient of 77.62 using 20% labeled data. Additionally, experiments on publicly available datasets further demonstrate the generalizability of the proposed method. The code is available at https://github.com/Oran9er/TFC-Net-main.

Machine Learning on Ultrasound Texture Analysis Data for Characterizing of Salivary Glandular Tumors: A Feasibility Study.

Objective quantitative texture characteristics may be helpful in salivary glandular tumor differential diagnosis. This study uses machine learning (ML) to explore and validate the performance of ultrasound (US) texture features in diagnosing salivary glandular tumors. 122 patients with salivary glandular tumors, including 71 benign and 51 malignant tumors, are enrolled. Representative brightness mode US pictures are selected for further Gray Level Co-occurrence Matrix (GLCM) texture analysis. We use a t-test to test the significance and use the receiver operating characteristic curve method to find the optimal cut-point for these significant features. After splitting 80% of the data into a training set and 20% data into a testing set, we use five machine learning models, k-nearest Neighbors (kNN), Naïve Bayes, Logistic regression, Artificial Neural Networks (ANNs) and supportive vector machine (SVM), to explore and validate the performance of US GLCM texture features in diagnosing salivary glandular tumors. This study includes 49 female and 73 male patients, with a mean age of 53 years old, ranging from 21 to 93. We find that six GLCM texture features (contrast, inverse difference movement, entropy, dissimilarity, inverse difference and difference entropy) are significantly different between benign and malignant tumors (p < 0.05). In ML, the overall accuracy rates are 74.3% (95%CI: 59.8-88.8%), 94.3% (86.6-100%), 72% (54-89%), 84% (69.5-97.3%) and 73.5% (58.7-88.4%) for kNN, Naïve Bayes, Logistic regression, a one-node ANN and SVM, respectively. US texture analysis with ML has potential as an objective and valuable tool to make a differential diagnosis between benign and malignant salivary gland tumors.

Surface roughness analysis of side-polished fiber based on the importance of texture features

In order to explore the use of side-polished fibre (SPF) for microprobe-type "lab-on-fibre", this study presents an analysis of the surface roughness in side-polished fiber (SPF) using the gray level co-occurrence matrix (GLCM) texture feature analysis method. Experimental results show that the flat areas of the SPF polished surface exhibit texture characteristics with high mean values in contrast and entropy, and low mean values in the angular second moment (ASM), homogeneity, and correlation. Employing the random forest (RF) feature importance ranking method based on the Gini coefficient and out-of-bag (OOB) error estimation, this study assesses the sensitivity of various GLCM texture parameters in classifying different roughness levels of the SPF polished surfaces. A feature subset comprising variance, ASM, entropy, and contrast is identified as optimal. Utilizing this subset, the paper conducts an RF classification validation experiment on the roughness of the SPF polished surfaces, with results showing an RF classification accuracy of 95.65%.This research provides evidence for exploring the impact of rough polished surfaces in SPF optic sensors on the light coupling mechanism with environmental materials and its influence on sensor sensitivity. It lays the foundation for exploring the precise identification of high-sensitivity areas on SPF polished surfaces.

Study on the EC prediction of cracked soda saline-alkali soil based on texture analysis of high-resolution images from ground-based observation and machine learning methods

The rapid and accurate measurement of soil salinity is essential for assessing the level of soil salinization. In natural conditions, the surface of cohesive soda saline-alkali soil experiences shrinkage and cracking phenomena during water evaporation. The propagation and development of these desiccation cracks can be effectively described using texture features due to their statistical randomness. This study aims to establish a relationship between electrical conductivity (EC) values and texture features derived from both gray-level co-occurrence matrix (GLCM) and wavelet decomposition, and to develop a prediction model for EC accordingly. To achieve these objectives, crack images on the surface of 200 soil samples with varying levels of salinity were obtained in the Songnen Plain field. GLCM was computed and wavelet decomposition was performed to extract texture features in different directions and scales. The results demonstrate a significant correlation between texture features and soil EC values. Among the various texture features, 12 GLCM texture features with a grayscale level of 2 and a step size of 1 pixel, and wavelet texture features derived from a 4th level orthogonal decomposition based on the coiflet-1 function were selected as the optimal parameters for establishing and comparing the predictive effects of two machine learning models on soil EC values. In comparison to the testing results of the BP neural network model (R2=0.83, RPD=1.64, RMSE=0.32 dS/m, MAE=0.18 dS/m), the random forest model exhibited higher accuracy and stability (R2=0.95, RPD=3.48, RMSE=0.21 dS/m, MAE=0.07 dS/m), indicating that although both machine learning models demonstrated rapid and nondestructive capabilities, the random forest method was better suited for EC prediction in soda saline-alkali soil due to its superior accuracy.

The object detection model uses combined extraction with KNN and RF classification

Object detection plays an important role in various fields. Developing detection models for 2D objects that experience rotation and texture variations is a challenge. In this research, the initial stage of the proposed model integrates the gray-level co-occurrence matrix (GLCM) and local binary patterns (LBP) texture feature extraction to obtain feature vectors. The next stage is classifying features using k-nearest neighbors (KNN) and random forest (RF), as well as voting ensemble (VE). System testing used a dataset of 4,437 2D images, the results for KNN accuracy were 92.7% and F1-score 92.5%, while RF performance was lower. Although GLCM features improve performance on both algorithms, KNN is more consistent. The VE approach provides the best performance with an accuracy of 93.9% and an F1-score of 93.8%, this shows the effectiveness of the ensemble technique in increasing object detection accuracy. This study contributes to the field of object detection with a new approach combining GLCM and LBP as feature vectors as well as VE for classification.

Enhanced convolutional neural network enabled optimized diagnostic model for COVID-19 detection

Computed tomography (CT) films are used to construct cross-sectional pictures of a particular region of the body by using many x-ray readings that were obtained at various angles. There is a general agreement in the medical community at this time that chest CT is the most accurate approach for identifying COVID-19 disease. It was demonstrated that chest CT had a higher sensitivity than reverse transcription polymerase chain reaction (RT-PCR) for the detection of COVID-19 illness. This article presents gray-level co-occurrence matrix (GLCM) texture feature extraction and convolutional neural network (CNN)-enabled optimized diagnostic model for COVID-19 detection. In this diagnostic model, CT scan images of patients are given as input. Firstly, GLCM algorithm is used to extract texture features from the CT scan images. This feature extraction helps in achieving higher classification accuracy. Classification is performed using CNN. It achieves higher accuracy than the k-nearest neighbors (KNN) algorithm and multi-layer preceptor (MLP). The accuracy of GLCM based CNN is 99%, F1 score is 99% and the recall rate is also 98%. CNN has achieved better results than MLP and KNN algorithms for COVID-19 detection.

Klasifikasi Rimpang Menggunakan Metode K-Nearest Neighbor dan Ekstraksi Ciri Gray Level Co-occurrence Matrix

Rhizome is a modification of plant stems that grow under the soil surface and function as a storage place for food reserves. This plants have internodes that function produce new shoots and roots. Rhizomes are commonly used by people as spices in cooking and herbal medicine. Rhizomes have many types, such as ginger, sand ginger, fingerroot, turmeric, galangal, and curcuma. These types have similarities to each other, such as texture, shape, and color. These similarities can cause problems such as difficulty in identifying the type of rhizome. The solution to this problem is a computer system that can classify the type of rhizomes. The system in this research was built using the K-Nearest Neighbor method and Gray Level Co-occurrence Matrix texture feature extraction. Research data amounted to 500 images with ginger, sand ginger, fingerroot, turmeric, and galangal classes. The stages of this research are data collection, image resizing, conversion to grayscale, GLCM feature extraction, storing the extraction results into dataframe, dividing data into train data and test data, classification with K-NN, and implement GUI to make operation easier. Accuracy results on this system get a value of 74% on test data and 64% on train data with value of K=11.

Textural indices of aerial images reveal urban landscapes temporal dynamics and their effects on plants and birds

Landscape temporal dynamics are a crucial concern in ecology to understand current biodiversity. But historical environmental data are often not easily available. This study assesses for the first time the potential of historical orthophotographs to describe past landscape conditions and determine the temporal lag between landscape changes and biotic communities responses.This method utilizes Gray level Co-occurrence Matrix (GLCM) texture indices computed from black and white orthophotographs to construct continuous metrics of landscape composition. Metrics were first developed using present-day data, i.e. 2015, and calibrated with categorical land cover maps of the Strasbourg Eurometropolis, France. Subsequently, these metrics were applied to historical orthophotographs from 1966, 1976, 1986, and 2000. Plant and bird data from research and citizen science programs were used to estimate the time delay in which these communities respond to evolutions in built areas and high vegetation.Obtained texture-based models reveal that built areas exhibit high contrast and homogeneity, depicted through a linear relation, and that high vegetation display low pixel brightness and high brightness diversity, better described via a nonlinear model. We successfully applied those findings to historical orthophotographs, and revealed dependencies on landscape composition up to 50 years ago for plants and up to 30 years ago for birds, with the time lag and the influence of built and high vegetation areas depending on the selected biodiversity indices.These results demonstrate the utility of archive black and white orthophotographs' texture indices for describing urban landscapes over the past five decades, making them valuable tools for ecological research. These indices are more accessible than categorical data like land cover maps for past years. They have the potential to greatly benefit future studies investigating time lags in landscape ecology, simplifying access to historical landscape features and contributing to sustainable urban planning efforts.

Mapping Maize Planting Densities Using Unmanned Aerial Vehicles, Multispectral Remote Sensing, and Deep Learning Technology

Maize is a globally important cereal and fodder crop. Accurate monitoring of maize planting densities is vital for informed decision-making by agricultural managers. Compared to traditional manual methods for collecting crop trait parameters, approaches using unmanned aerial vehicle (UAV) remote sensing can enhance the efficiency, minimize personnel costs and biases, and, more importantly, rapidly provide density maps of maize fields. This study involved the following steps: (1) Two UAV remote sensing-based methods were developed for monitoring maize planting densities. These methods are based on (a) ultrahigh-definition imagery combined with object detection (UHDI-OD) and (b) multispectral remote sensing combined with machine learning (Multi-ML) for the monitoring of maize planting densities. (2) The maize planting density measurements, UAV ultrahigh-definition imagery, and multispectral imagery collection were implemented at a maize breeding trial site. Experimental testing and validation were conducted using the proposed maize planting density monitoring methods. (3) An in-depth analysis of the applicability and limitations of both methods was conducted to explore the advantages and disadvantages of the two estimation models. The study revealed the following findings: (1) UHDI-OD can provide highly accurate estimation results for maize densities (R2 = 0.99, RMSE = 0.09 plants/m2). (2) Multi-ML provides accurate maize density estimation results by combining remote sensing vegetation indices (VIs) and gray-level co-occurrence matrix (GLCM) texture features (R2 = 0.76, RMSE = 0.67 plants/m2). (3) UHDI-OD exhibits a high sensitivity to image resolution, making it unsuitable for use with UAV remote sensing images with pixel sizes greater than 2 cm. In contrast, Multi-ML is insensitive to image resolution and the model accuracy gradually decreases as the resolution decreases.

Estimating Winter Wheat Plant Nitrogen Content by Combining Spectral and Texture Features Based on a Low-Cost UAV RGB System throughout the Growing Season

As prior information for precise nitrogen fertilization management, plant nitrogen content (PNC), which is obtained timely and accurately through a low-cost method, is of great significance for national grain security and sustainable social development. In this study, the potential of the low-cost unmanned aerial vehicle (UAV) RGB system was investigated for the rapid and accurate estimation of winter wheat PNC across the growing season. Specifically, texture features were utilized as complements to the commonly used spectral information. Five machine learning regression algorithms, including support vector machines (SVMs), classification and regression trees, artificial neural networks, K-nearest neighbors, and random forests, were employed to establish the bridge between UAV RGB image-derived features and ground-truth PNC, with multivariate linear regression serving as the reference. The results show that both spectral and texture features had significant correlations with ground-truth PNC, indicating the potential of low-cost UAV RGB images to estimate winter wheat PNC. The H channel, S4O6, and R_SE and R_EN had the highest correlation among the spectral indices, Gabor texture features, and grey level co-occurrence matrix texture features, with absolute Pearson’s correlation coefficient values of 0.63, 0.54, and 0.69, respectively. When the texture features were used together with spectral indices, the PNC estimation accuracy was enhanced, with the root mean square error (RMSE) decreasing from 2.56 to 2.24 g/kg, for instance, when using the SVM regression algorithm. The SVM regression algorithm with validation achieved the highest estimation accuracy, with a coefficient of determination (R2) of 0.62 and an RMSE of 2.15 g/kg based on the optimal feature combination of B_CON, B_M, G_DIS, H, NGBDI, R_EN, R_M, R_SE, S3O7, and VEG. Overall, this study demonstrated that the low-cost UAV RGB system could be successfully used to map the PNC of winter wheat across the growing season.

Frequency and Spatial Domain-Based Approaches for Recognition of Indian Sign Language Gestures

Objectives: The objective of this paper is to introduce and demonstrate an innovative approach for the recognition of Indian sign language gestures, with a focus on bridging communication gap between the deaf and hearing communities. The goal is to contribute to the development of effective tools and technologies that facilitate seamless communication between individuals using sign language and the people with no knowledge about sign language. Methods: The methodology consists of three key steps. First, data pre-processing involves resizing and contours extraction. Next, feature extraction employs Fourier descriptors for frequency domain analysis and gray-level-co-occurrence matrix for spatial domain analysis. Finally, various machine learning models including SVM, Random Forest, Logistic Regression, K-Nearest Neighbor and Naive Bayes are trained on a standard dataset. Findings: In our controlled experimental setup, we applied a diverse set of machine learning classifiers to evaluate the proposed approach for gesture recognition. Among the classifiers tested, K-Nearest Neighbors demonstrated the highest accuracy, achieving 99.82%. To validate the robustness of our approach, we employed k-fold cross-validation with 5 folds. Novelty: This study presents an innovative method for sign language recognition by employing a dual-domain fusion strategy that prominently emphasizes the frequency domain. Through the integration of Fourier descriptors, the research conducts a detailed frequency domain analysis to characterize the contour shapes of sign language gestures. The synergy with gray-level co-occurrence matrix texture features in the spatial domain analysis, contributes to the creation of a comprehensive feature vector. The proposed approach ensures a thorough exploration of gesture features, there by advancing the precision and efficacy of sign language recognition. Keywords: Indian Sign Language (ISL), Sign Language Recognition (SLR), Frequency domain, Spatial domain, Fourier descriptors, Gray level co­occurrence matrix (GLCM), K­ Fold

Optimizing window size and directional parameters of GLCM texture features for estimating rice AGB based on UAVs multispectral imagery.

Aboveground biomass (AGB) is a crucial physiological parameter for monitoring crop growth, assessing nutrient status, and predicting yield. Texture features (TFs) derived from remote sensing images have been proven to be crucial for estimating crops AGB, which can effectively address the issue of low accuracy in AGB estimation solely based on spectral information. TFs exhibit sensitivity to the size of the moving window and directional parameters, resulting in a substantial impact on AGB estimation. However, few studies systematically assessed the effects of moving window and directional parameters for TFs extraction on rice AGB estimation. To this end, this study used Unmanned aerial vehicles (UAVs) to acquire multispectral imagery during crucial growth stages of rice and evaluated the performance of TFs derived with different grey level co-occurrence matrix (GLCM) parameters by random forest (RF) regression model. Meanwhile, we analyzed the importance of TFs under the optimal parameter settings. The results indicated that: (1) the appropriate window size for extracting TFs varies with the growth stages of rice plant, wherein a small-scale window demonstrates advantages during the early growth stages, while the opposite holds during the later growth stages; (2) TFs derived from 45° direction represent the optimal choice for estimating rice AGB. During the four crucial growth stages, this selection improved performance in AGB estimation with R2 = 0.76 to 0.83 and rRMSE = 13.62% to 21.33%. Furthermore, the estimation accuracy for the entire growth season is R2 =0.84 and rRMSE =21.07%. However, there is no consensus regarding the selection of the worst TFs computation direction; (3) Correlation (Cor), Mean, and Homogeneity (Hom) from the first principal component image reflecting internal information of rice plant and Contrast (Con), Dissimilarity (Dis), and Second Moment (SM) from the second principal component image expressing edge texture are more important to estimate rice AGB among the whole growth stages; and (4) Considering the optimal parameters, the accuracy of texture-based AGB estimation slightly outperforms the estimation accuracy based on spectral reflectance alone. In summary, the present study can help researchers confident use of GLCM-based TFs to enhance the estimation accuracy of physiological and biochemical parameters of crops.

The effects of 20-hydroxyecdysone on nuclear shape, heterochromatin quantity and gray-level co-occurrence matrix texture analysis of adrenal zona fasciculata cells in an obese gerbil (Gerbillus tarabuli) model for metabolic syndrome: a correlational study.

The aim of this study was to reveal the effects of obesity and phytotherapy with 20-hydroxyecdysone (20E) on the nuclei of adrenal zona fasciculata (ZF) in the gerbil Gerbillus tarabuli by analyzing nuclear shape and gray-level co-occurrence matrix (GLCM) texture characteristics and by quantifying heterochromatin. Twelve gerbils were divided into three groups: control (C), HC and HC-20E (animals receiving a high-calorie-diet without or with a supplement of 20E, respectively). The adrenals were removed and fixed for histological and statistical analysis. Principal component analysis showed a positive correlation of area, perimeter and textural correlation in C. Nevertheless, a negative correlation was recorded for contrast and entropy. The obesity caused a disorder in nuclear texture; negative correlation was noted with heterochromatin fraction, which may be related to increased ZF activity. However, administration of 20E seems to improve the nuclear state by preserving circularity, uniformity and homogeneity of nuclei as well as the proportion of heterochromatin, which could be a sign of a downregulation of cell activity.Our results suggest that new techniques of image processing could contribute to the understanding of nuclear changes associated with obesity and its possible therapy in this gerbil model for metabolic syndrome.

Can texture analysis of T2-weighted MRI be used to predict extrathyroidal extension in papillary thyroid carcinoma?

Determining the presence of extrathyroidal extension (ETE) is important for established of different surgical protocol and postoperative patient management in patients with papillary thyroid carcinoma (PTC). The correlation relationship between texture features from T2-weighted imaging (T2WI) and ETE has not been explored extensively. This study aimed to explore the value of T2-weighted magnetic resonance imaging - based whole tumor texture analysis in predict extrathyroidal extension with PTC. In this retrospectively study, 76 patients with pathologically proven PTC were recruited, who received surgical resection and underwent preoperative thyroid magnetic resonance imaging. Based on histo-pathologically findings, patients were classified into ETE and no ETE groups. ETE group was further divided into 2 subgroups (minimal ETE and extensive ETE). Whole-tumor texture analysis was independently performed by 2 radiologists on axial T2WI images. Nine histogram and gray-level co-occurrence matrix (GLCM) texture features were automatically extracted. Univariate and multivariate analysis were performed to determine risk factors associated with ETE. Predictive performance was evaluated by receiver operating characteristic (ROC) analysis. Interobserver agreement, confirmed by intraclass correlation coefficients (ICCs) ranging from 0.78 to 0.89, was excellent for texture analysis between 2 radiologists. T2WI image derived entropy, standard deviation, energy and correlation have significant difference between PTC with and without ETE (all P < .05). Among these, entropy showed the best diagnostic efficiency with the area under ROC curve of 0.837, diagnostic threshold of 5.86, diagnostic sensitivity and specificity of 81.5% and 75.6%, respectively. Additionally, the multivariate analysis revealed that high entropy was an independent risk factor of ETE (odds ratio, OR = 19.348; 95%CI, 4.578-81.760; P = .001). The findings indicate a significant association between texture features of the primary tumor based on T2WI and the presence of ETE in PTC. These results have the potential to help predict ETE preoperatively in patients with PTC, offering valuable insights for clinical decision-making.

Above ground tree biomass modeling using machine learning algorithms in western Terai Sal Forest of Nepal

The monitoring of forest biomass is a crucial biophysical parameter in forest ecosystems, as it provides valuable information for managing forests sustainably and tracking carbon circulation statistics. To achieve sustainable forest management, it is essential to monitor and study forest resources, particularly biomass. This study aimed to model above ground tree biomass (AGTB) using Machine Learning Algorithms (MLAs) in the western terai Sal forest of Nepal. AGTB was calculated using a systematic inventory sample plot, while spectral and textural variables were processed and masked for the study area using Sentinel-2A satellite imagery. Three MLAs namely support vector machine (SVM), random forest (RF), and stochastic gradient boosting (SGB), were employed for modeling with eight categorized variable datasets. Among the MLAs, the RF algorithm with a combination of gray-level co-occurrence matrix (GLCM) and raw bands (RB) dataset variable demonstrated the best performance, with a low RMSE value of 78.81 t ha−1 in the test data. However, the AGTB range from this model ranged from 118.34 to 425.97 t ha−1. The study found that traditional indices, raw bands, and GLCM texture from near-infrared were important variables for AGTB. Nevertheless, the RF algorithm and the dataset combination of GLCM plus raw bands (RB) exhibited excellent performance in all model runs. Thus, this pioneering study on comparative MLAs-based AGTB assessment with multiple datasets variables can provide valuable insights for new researchers and the development of novel approaches for biomass/carbon estimation techniques in Nepal.

A comparative study of Machine Learning-based classification of Tomato fungal diseases: Application of GLCM texture features

Globally, agriculture remains an important source of food and economic development. Due to various plant diseases, farmers continue to suffer huge yield losses in both quality and quantity. In this study, we explored the potential of using Artificial Neural Networks, K-Nearest Neighbors, Random Forest, and Support Vector Machine to classify tomato fungal leaf diseases: Alternaria, Curvularia, Helminthosporium, and Lasiodiplodi based on Gray Level Co-occurrence Matrix texture features. Small differences between symptoms of these diseases make it difficult to use the naked eye to obtain better results in detecting and distinguishing these diseases. The Artificial Neural Network outperformed other classifiers with an overall accuracy of 94% and average scores of 93.6% for Precision, 93.8% for Recall, and 93.8% for F1-score. Generally, the models confused samples originally belonging to Helminthosporium with Curvularia. The extracted texture features show great potential to classify the different tomato leaf fungal diseases. The results of this study show that texture characteristics of the Gray Level Co-occurrence Matrix play a critical role in the establishment of tomato leaf disease classification systems and can facilitate the implementation of preventive measures by farmers, resulting in enhanced yield quality and quantity.

Physics-Informed Discretization for Reproducible and Robust Radiomic Feature Extraction Using Quantitative MRI.

Given the limited repeatability and reproducibility of radiomic features derived from weighted magnetic resonance imaging (MRI), there may be significant advantages to using radiomics in conjunction with quantitative MRI. This study introduces a novel physics-informed discretization (PID) method for reproducible radiomic feature extraction and evaluates its performance using quantitative MRI sequences including magnetic resonance fingerprinting (MRF) and apparent diffusion coefficient (ADC) mapping. A multiscanner, scan-rescan dataset comprising whole-brain 3D quantitative (MRF T1, MRF T2, and ADC) and weighted MRI (T1w MPRAGE, T2w SPACE, and T2w FLAIR) from 5 healthy subjects was prospectively acquired. Subjects underwent 2 repeated acquisitions on 3 distinct 3 T scanners each, for a total of 6 scans per subject (30 total scans). First-order statistical (n = 23) and second-order texture (n = 74) radiomic features were extracted from 56 brain tissue regions of interest using the proposed PID method (for quantitative MRI) and conventional fixed bin number (FBN) discretization (for quantitative MRI and weighted MRI). Interscanner radiomic feature reproducibility was measured using the intraclass correlation coefficient (ICC), and the effect of image sequence (eg, MRF T1 vs T1w MPRAGE), as well as image discretization method (ie, PID vs FBN), on radiomic feature reproducibility was assessed using repeated measures analysis of variance. The robustness of PID and FBN discretization to segmentation error was evaluated by simulating segmentation differences in brainstem regions of interest. Radiomic features with ICCs greater than 0.75 following simulated segmentation were determined to be robust to segmentation. First-order features demonstrated higher reproducibility in quantitative MRI than weighted MRI sequences, with 30% (n = 7/23) features being more reproducible in MRF T1 and MRF T2 than weighted MRI. Gray level co-occurrence matrix (GLCM) texture features extracted from MRF T1 and MRF T2 were significantly more reproducible using PID compared with FBN discretization; for all quantitative MRI sequences, PID yielded the highest number of texture features with excellent reproducibility (ICC > 0.9). Comparing texture reproducibility of quantitative and weighted MRI, a greater proportion of MRF T1 (n = 225/370, 61%) and MRF T2 (n = 150/370, 41%) texture features had excellent reproducibility (ICC > 0.9) compared with T1w MPRAGE (n = 148/370, 40%), ADC (n = 115/370, 32%), T2w SPACE (n = 98/370, 27%), and FLAIR (n = 102/370, 28%). Physics-informed discretization was also more robust than FBN discretization to segmentation error, as 46% (n = 103/222, 46%) of texture features extracted from quantitative MRI using PID were robust to simulated 6 mm segmentation shift compared with 19% (n = 42/222, 19%) of weighted MRI texture features extracted using FBN discretization. The proposed PID method yields radiomic features extracted from quantitative MRI sequences that are more reproducible and robust than radiomic features extracted from weighted MRI using conventional (FBN) discretization approaches. Quantitative MRI sequences also demonstrated greater scan-rescan robustness and first-order feature reproducibility than weighted MRI.

Texture feature analysis of MRI-ADC images to differentiate glioma grades using machine learning techniques

Apparent diffusion coefficient (ADC) of magnetic resonance imaging (MRI) is an indispensable imaging technique in clinical neuroimaging that quantitatively assesses the diffusivity of water molecules within tissues using diffusion-weighted imaging (DWI). This study focuses on developing a robust machine learning (ML) model to predict the aggressiveness of gliomas according to World Health Organization (WHO) grading by analyzing patients’ demographics, higher-order moments, and grey level co-occurrence matrix (GLCM) texture features of ADC. A population of 722 labeled MRI-ADC brain image slices from 88 human subjects was selected, where gliomas are labeled as glioblastoma multiforme (WHO-IV), high-grade glioma (WHO-III), and low-grade glioma (WHO I-II). Images were acquired using 3T-MR systems and a region of interest (ROI) was delineated manually over tumor areas. Skewness, kurtosis, and statistical texture features of GLCM (mean, variance, energy, entropy, contrast, homogeneity, correlation, prominence, and shade) were calculated using ADC values within ROI. The ANOVA f-test was utilized to select the best features to train an ML model. The data set was split into training (70%) and testing (30%) sets. The train set was fed into several ML algorithms and selected most promising ML algorithm using K-fold cross-validation. The hyper-parameters of the selected algorithm were optimized using random grid search technique. Finally, the performance of the developed model was assessed by calculating accuracy, precision, recall, and F1 values reported for the test set. According to the ANOVA f-test, three attributes; patient gender (1.48), GLCM energy (9.48), and correlation (13.86) that performed minimum scores were excluded from the dataset. Among the tested algorithms, the random forest classifier(0.8772 ± 0.0237) performed the highest mean-cross-validation score and selected to build the ML model which was able to predict tumor categories with an accuracy of 88.14% over the test set. The study concludes that the developed ML model using the above features except for patient gender, GLCM energy, and correlation, has high prediction accuracy in glioma grading. Therefore, the outcomes of this study enable to development of advanced tumor classification applications that assist in the decision-making process in a real-time clinical environment.