The hydrological conditions of the Amarasi District, Kupang Regency have 3-4 wet months and 8-9 dry months according to Oldeman’s classification. Annual rainfall in the last ten years shows the lowest rainfall was 1,461mm and the highest was 2,688mm, the average annual rainfall was 1,859mm. The Amarasi region is included in zones D and E of the Oldeman climate classification. The practice of utilizing limited water resources by planting fodder crops in the form of legumes and grasses as well as food crops in integrated dry land agriculture has been carried out by the Amarasi community. This research examines the availability and demand for domestic, agricultural and livestock water and will produce an availability index as the carrying capacity of regional water availability. The method used to calculate the availability of water originating from rain is runoff analysis based on weighted coefficients for each land use, then analyzed in a monthly series and compared with the level of water demand for each use. Domestic water needs, livestock drinking and irrigation follow SNI 19-6728.1.2002. Then the analysis results are interpreted with tables and time series graphs. The research results show the following time series of water availability: January: 44,626,635.13m3; February: 31,210,646.01m3; March : 20,050,098.53m3; April: 11,726,074.47m3; May : 3,023,180.66m3; June: 1,747,689.52m3; July : 973,284.18m3; August : 168,880.36m3; September: 1,026,614.82m3. October 2,846,522.91m3; November 10,713,903.37m3; and December: 26,871,976.23m3. The amount of water demand in the time series is as follows: January: 1,929,491.70m3; February: 1,391,817.98m3; March: 1,697,543.42m3; April: 781,567.95m3; May : 883,736.22m3; June: 755,911.95m3; July : 284,777.42m3; August : 384,569.42m3; September: 387,871.95m3. October 355,448.02m3; November 3,242,283.15m3; and December: 2,631,159.26m3. Water availability index: January: 4.32 (good), February: 4.46 (good), March: 8.47 (good), April: 6.67 (good), May: 29.23 (slight critical), June: 43.25 (mild critical), July: 29.26 (mild critical), August: 227.27 (bad/severe critical), September: 37.78 (mild critical), October 12.49 (good), November: 30.26 (mild critical) and December 9.79 (good).