The smallest positive eigenvalue τ ( G ) of a simple graph G is the smallest positive eigenvalue of its adjacency matrix A ( G ) . In [F. J. Zhang and A. Chang, Acyclic molecules with greatest HOMO-LUMO separation, Discrete Applied Mathematics, 98:165–171, (1999).], the authors characterized all nonsingular trees with τ equal to 2 − 1 . We consider the same problem for bipartite unicyclic graphs with a unique perfect matching. Let U be the class of all connected bipartite unicyclic graphs with a unique perfect matching. In this article, we characterize all graphs U in U with the property that τ ( U ) = 2 − 1 . Further, we show that the largest limit point of the smallest positive eigenvalues of graphs in U is 2 − 1 , whereas the smallest limit point is 0.