Grapefruit Oil Research Articles

Development and Evaluation of Oromucosal Spray Formulation Containing Plant-Derived Compounds for the Treatment of Infectious and Inflammatory Diseases of the Oral Cavity.

According to data in the literature, natural products and essential oils are often used in dental practice. To develop a new oromucosal spray for the treatment of infectious and inflammatory diseases of the oral cavity, clove CO2 extract and essential oils of lavender and grapefruit were used as active pharmaceutical ingredients. Clove extract was obtained by the method of subcritical extraction from various raw materials, the choice of which was based on the yield of the CO2 extract and the study of its phytochemical and microbiological properties. Based on the results of microscopic and diffraction analyses, the rational time of ultrasonic exposure for the emulsion of active pharmaceutical ingredients was established. Mucoadhesive polymers were used as stabilizers of the two-phase system and prolongators. This article discusses the impact of the type and concentration of mucoadhesive polymers on the stability of the emulsion system; the viscous, textural, adhesive, and film characteristics of oromucosal spray; and the parameters determining sprayability.

Determination of Limonene Chirality in Oils Obtained from Different Types of Citrus Waste Peels in Türkiye

Limonene constitutes a significant amount in citrus oils. It has a chiral structure and has two different optically active isomers, R-limonene and S-limonene, which are symmetrical to each other. Determining the chiral configurations of limonene plays an important role in determining the beneficial use areas of essential oils. Citrus oils are used in a wide variety of industrial areas, depending on their limonene content. This paper presents the analytical method optimization, validation, and chirality studies of limonene in the citrus oils acquired from different citrus waste peels in Türkiye. An inlet temperature of 250 °C and an injection volume of 2 µL were decided as the optimal conditions for the most accurate measurement of both limonenes in the citrus oil. This method produced results for linearity, sensitivity (LODs and LOQs), repeatability, and reproducibility that were acceptable within the scope of the validation studies. The chirality of limonene was investigated in twenty-six citrus oils (fifteen orange oils, six lemon oils, four mandarin oils, and one grapefruit oil) in Türkiye. While the content of R-limonene in orange oil varied between 56.39% and 72.85%, the content of S-limonene changed from 2.53% to 5.71%. Whereas the constituent of R-limonene in lemon oils ranged from 54.73% to 73.99%, the content of S-limonene varied between 3.78-4.79%. In mandarin oils, the content of R-limonene was determined to be 58.02% and 65.05%, while the content of S-limonene was found as 3.05% and 4.87%. In single grapefruit oil, R-limonene content was 60.69% and S-limonene content was 3.12%.

Citrus Essential Oils as Potential Insecticides Against Thrips flavus

Background: Essential oils (EOs) are recognized for their potent insecticidal activity and are widely considered promising agents for pest control. Methods: The insecticidal activity of seven commercial citrus EOs against the global phytophagous pest Thrips flavus was evaluated using bioassays conducted under laboratory conditions. Moreover, the chemical composition of these EOs was analyzed using gas chromatography-mass spectrometry (GC-MS). Results: Among the 45 identified compounds, the major constituents were d-limonene (23.77%– 95.10%), methyl jasmonate (38.76%), and linalyl acetate (34.55%). Orange flower oil, sweet orange oil, tangerine peel oil, bergamot oil, lime oil, lemon oil, and grapefruit oil exhibited good insecticidal activity against T. flavus without demonstrating significant repellent activity. Orange flower oil and sweet orange oil showed higher insecticidal toxicity, with LC50 values of 0.20 g/L and 0.37 g/L, respectively. In pot experiments, the higher control efficacy against T. flavus was found after 7 days of treatment for orange flower oil (91.14±1.27% at 720.00 g a.i.·hm−2 and 100% at 900.00 g a.i.·hm−2), sweet orange oil (91.14±5.52% at 900.00 g a.i.·hm−2), tangerine peel oil (96.20±2.19% at 720 g a.i.·hm−2 and 100% at 900.00 g a.i.·hm−2), lemon oil (96.20±2.19% at 900 g a.i.·hm−2), and lime oil (97.47±2.53% at 900.00 g a.i.·hm−2). Conclusion: Taken together, orange flower oil and tangerine peel oil, which were found to contain d-limonene and methyl jasmonate, demonstrated rapid and more effective insecticidal activity compared to the other EOs tested, which makes these two EOs promising alternatives to chemical insecticides.

A Comparative GC/MS Analysis of Citrus Essential Oils: Unveiling the Potential Benefits of Herb-Drug Interactions in Preventing Paracetamol-Induced Hepatotoxicity.

Our study aimed to test the potential of Citrus oils in protecting against paracetamol (PAR)-induced hepatotoxicity. The essential oils of Pineapple sweet orange (OO), Murcott mandarin (MO), Red grapefruit (GO), and Oval kumquat (KO) were investigated using gas chromatography coupled with mass spectrometry (GC/MS). Twenty-seven compounds were identified, with monoterpene hydrocarbons being abundant class. d-Limonene had the highest percentage (92.98 %, 92.82 %, 89.75 %, and 94.46 % in OO, MO, GO, and KO, respectively). Hierarchical cluster analysis (HCA) and principal components analysis (PCA) revealed that octanal, linalool, germacrene D, and d-limonene were the principal discriminatory metabolites that segregated the samples into three distinct clusters. In vitro antioxidant capacities were ranged from 1.2-12.27, 1.79-5.91, and 235.05-585.28 μM Trolox eq/mg oil for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic (ABTS), ferric-reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC), respectively. In vivo, citrus oils exhibited a significant reduction in alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and nitric oxide (NO). Additionally, there was an increase in glutathione reductase (GSH), and the liver architecture was nearly normal. Molecular docking revealed that d-limonene exhibited a good inhibitory interaction with cytochrome P450 (CYP450) isoforms 1A2, 3A4, and 2E1, with binding energies of -6.17, -4.51, and -5.61 kcal/mol, respectively.

Effect of Commonly Used Cosmetic Preservatives on Healthy Human Skin Cells

Cosmetic products contain preservatives to prevent microbial growth. The various types of preservatives present in skincare products applied on the skin induce many side effects. We tested several types of preservatives such as phenoxyethanol, methyl paraben, propyl paraben, imidazolidinyl urea (IU), the composition of gluconolactone and sodium benzoate (GSB), diazolidinyl urea (DU), and two grapefruit essential oils, one of which was industrially produced and a second which was freshly distilled from fresh grapefruit peels. This study aimed to find the relationship between preservative concentration, cell growth, collagen secretion, and cell viability. We hypothesized that these products induced a decrease in collagen secretion from human dermal fibroblasts. Our research, for the first time, addressed the overall effect of other preservatives on skin extracellular matrix (ECM) by studying their effect on metalloproteinase-2 (MMP-2) activity. Except for cytotoxicity and contact sensitivity tests, there are no studies of their effect on skin ECM in the available literature. These studies show potential antimicrobial activity, especially from the compounds IU and DU towards reference bacteria and the compounds methyl paraben and propyl paraben against reference fungi. The MTS test showed that fibroblasts are more sensitive to the tested group of preservatives than keratinocytes, which could be caused by the differences between the cells' structures. The grapefruit oils exhibited the most cytotoxicity to both tested cell lines compared to all considered preservatives. The most destructive influence of preservatives on collagen synthesis was observed in the case of IU and DU. In this case, the homemade grapefruit oil turned out to be the mildest one. The results from a diverse group of preservatives show that whether they are natural or synthesized compounds, they require controlled use. Appropriate dosages and evaluation of preservative efficacy should not be the only aspects considered. The complex effect of preservatives on skin processes and cytotoxicity is an important topic for modern people.

Data analysis of the corrosion protection behavior of ginger, tea tree and grapefruit essential oil extracts on low carbon steel in H2SO4 solution

Application of corrosion inhibitors are the most versatile corrosion prevention methods of carbon steels in corrosive environments. Research on non-toxic chemical compounds are ongoing and results from previous study have proven the effectiveness of the compounds. Data on corrosion inhibition of ginger (GG), tea tree (TR) and grapefruit (GF) oil extracts on low carbon steel in 0.5 M H2SO4 are presented. The extracts performed effectively with optimal values of 99.56 %, 98.17 % and 98.32 % at 2.5 % GG, 3.5 % TR and 3 % GF concentrations. Corresponding corrosion rate values are 0.28 mm/y, 1.16 mm/y and 1.49 mm/y. Corrosion rate at 0 % extract concentration is 63.33 mm/y. Performance of TR extract significantly varies with time and concentration with statistical value of 48.21 % and 23.02 %. GG and GF concentration where the only statistically relevant factors for GG and GF performance with statistical values of 68.42 % and 73.20 %. Standard deviation data for GG extracts varied minimally from mean values compared to TR and GF extracts. Results shows 92 %, 18 % and 63 % of GG, TR and GF extracts inhibition data are above 95 % inhibition value at margin of error of 6.99 %, 10 % and 12.19 %.

The Use of Biopolymers as a Natural Matrix for Incorporation of Essential Oils of Medicinal Plants.

The benefits of using biopolymers for the development of films and coatings are well known. The enrichment of these material properties through various natural additions has led to their applicability in various fields. Essential oils, which are well-known for their beneficial properties, are widely used as encapsulating agents in films based on biopolymers. In this study, we developed biopolymer-based films and tested their properties following the addition of 7.5% and 15% (w/v) essential oils of lemon, orange, grapefruit, cinnamon, clove, chamomile, ginger, eucalyptus or mint. The samples were tested immediately after development and after one year of storage in order to examine possible long-term property changes. All films showed reductions in mass, thickness and microstructure, as well as mechanical properties. The most considerable variations in physical properties were observed in the 7.5% lemon oil sample and the 15% grapefruit oil sample, with the largest reductions in mass (23.13%), thickness (from 109.67 µm to 81.67 µm) and density (from 0.75 g/cm3 to 0.43 g/cm3). However, the microstructure of the sample was considerably improved. Although the addition of lemon essential oil prevented the reduction in mass during the storage period, it favored the degradation of the microstructure and the loss of elasticity (from 16.7% to 1.51% for the sample with 7.5% lemon EO and from 18.28% to 1.91% for the sample with 15% lemon EO). Although the addition of essential oils of mint and ginger resulted in films with a more homogeneous microstructure, the increase in concentration favored the appearance of pores and modifications of color parameters. With the exception of films with added orange, cinnamon and clove EOs, the antioxidant capacity of the films decreased during storage. The most obvious variations were identified in the samples with lemon, mint and clove EOs. The most unstable samples were those with added ginger (95.01%), lemon (92%) and mint (90.22%).

The Future Packaging of the Food Industry: The Development and Characterization of Innovative Biobased Materials with Essential Oils Added.

The need to replace conventional, usually single-use, packaging materials, so important for the future of resources and of the environment, has propelled research towards the development of packaging-based on biopolymers, fully biodegradable and even edible. The current study furthers the research on development of such films and tests the modification of the properties of the previously developed biopolymeric material, by adding 10, respectively 20% w/v essential oils of lemon, grapefruit, orange, cinnamon, clove, mint, ginger, eucalypt, and chamomile. Films with a thickness between 53 and 102 µm were obtained, with a roughness ranging between 147 and 366 nm. Most films had a water activity index significantly below what is required for microorganism growth, as low as 0.27, while all essential oils induced microbial growth reduction or 100% inhibition. Tested for the evaluation of physical, optical, microbiological or solubility properties, all the films with the addition of essential oil in the composition showed improved properties compared to the control sample.

Grapefruit Oil and Cobalt Nitrate-Loaded Polyurethane Hybrid Nanofibrous Scaffold for Biomedical Applications

The goal of this work is to fabricate a new composite based on polyurethane (PU), grapefruit (GP) oil, and cobalt nitrate [Co(NO3)2] using an electrospinning technique. Morphology results revealed the reduction in the fiber diameter of the composites compared to pristine PU control. The interaction of PU with GP and Co(NO3)2 was confirmed by hydrogen bond formation evident in infrared analysis. The fabricated PU/GP composites depicted a more hydrophobic behavior, while PU/GP/Co(NO3)2 showed a hydrophilic behavior than the pristine PU. Atomic force micrographs (AFM) revealed that the developed composites showed a decrease in the surface roughness (Ra) compared to PU. The addition of GP and Co(NO3)2 improved the mechanical strength of the pristine PU. The blood compatibility assays concluded not only the increase in blood clotting levels but also the less toxic nature of the fabricated composites compared to the pristine PU. Hence, the newly designed composites possessing outstanding physicochemical and biological properties may be used as a potential candidate for scaffolding in tissue engineering applications.

Synergistic effect of essential oil distillates on the corrosion protection of GX4CrNiMo16-5-1 stainless in acid-chloride electrolyte

The inhibition capability of admixed rosemary oil with cinnamon cassia oil (RC), and grapefruit oil with lemongrass oil (GL) on GX4CrNiMo16-5-1 martensitic stainless steel (GX4ST) corrosion was studied in 6 M H2SO4/3.5% NaCl electrolyte by potentiodynamic polarization and optical microscopy characterization. Results show both admixed distillates performed effectively on GX4ST. The corrosion rate of GX4ST without the distillates at 2.82 mm/y significantly decreased to average value of 0.04 mm/y and 0.05 mm/y analogous to average inhibition efficiency of 98.64% and 98.23% for RC and GL compound. The oil distillates exhibit consistent anodic corrosion inhibition behavior at all concentrations studied. Corrosion thermodynamic calculations showed the distillates chemisorbed on the martensitic steel with Gibbs free energy values greater than -46.01 Kjmol−1 with respect to Langmuir isotherm equation which indicates negligible of lateral interaction effect among inhibitor molecules and surface coverage protection mechanism. Optical image representations of GX4ST after corrosion without the distillates showed severe surface degradation of general and localized corrosion. This contrast the images of the inhibited steel surface which were closely similar to the steel image before corrosion.

Isopulegol: A Promising Phytochemical with Potential Therapeutic Benefits

Phytochemicals are naturally occurring biologically active compounds that provide promising options to existing therapeutic approaches and serve as vital resources for novel drugs. Essential oils derived from various medicinal plants contain a wide array of phytochemicals that exhibit diverse pharmacological properties. Terpenes are low molecular weight, volatile compounds that are abundant in essential oil of various plants and possess useful biological activity for treating diseases and ailments. Isopulegol is a monoterpene alcohol present in the essential oils of grape fruit, mint and rosemary. This mini review summarizes recent progress on the health benefits of isopulegol and discusses its potential mechanisms in the prevention and treatment of diseases in various experimental models.

MICROENCAPSULATION of grapefruit oil with sodium alginate by gelation and ionic extrusion: optimization and modeling of crosslinking and study of controlled release kinetics.

Essential grapefruit oil with high concentrations of limonene is used in food, cosmetic and pharmaceutical industries due to its antimicrobial properties, fragrance, and flavor. To facilitate its manipulation and protect it from adverse environmental factors, the microencapsulation is used. The objective of this work was to optimize the microencapsulation process of grapefruit oil using external ionic gelation coupled to extrusion with sodium alginate and calcium chloride. We achieved the best encapsulation conditions with calcium chloride concentration at 7.4% w/v and a crosslinking time of 58 minutes, obtaining a yield of 62% and an efficiency of 100% with an oil loading capacity of 10% w/w. The chemical adsorption of calcium as well during the crosslinking process was studied, observing a significant fit with the Elovich equation. And an adjustment of the controlled release of the oil was obtained to the empirical kinetic model of Korsmeyer and Peppas.

Optimization of the endodontic retreatment

Aim. Optimization of an endodontic retreatment by performing the following tasks, including the selection of an optimal and safe solvent.Materials and methods. In order to choose the most suitable solvent for the problem, an experiment was conducted with clove, mint, eucalyptus, orange, and grapefruit oils. The surface tension coefficient was measured by the Rebinder apparatus. In order to justify the choice of an effective essential oil, as well as the dissolving ability and the absence of solvent and debris after irrigation with sodium hypochlorite in the root canal, a tube-shaped electron scanning microscopy was performed.Results. Grapefruit oil has the most dissolving activity (p < 0.05). In addition to the solubility of the filler and siller, grapefruit essential oil is able to dissolve the smear layer.Conclusion. Optimization of the endodontic retreatment with root canals previously filled with gutta-percha, is possible using essential oils, however, a grapefruit oil is the most effective.

Integrated Methods of Control of Body Weight, Appetitis and Food Behavior Using Short-Term Inhalations of High-Concentrated Oxygen with Adding of Grapefruit Oil

Integrated Methods of Control of Body Weight, Appetitis and Food Behavior Using Short-Term Inhalations of High-Concentrated Oxygen with Adding of Grapefruit Oil

Efficacy of eucalyptus oil (Eucalyptus globulus), sweet orange oil (Citrus sinensis), and grapefruit oil (Citrus paradisi) as bioceramic sealer solvents

Background: Essential oil–based gutta-percha solvents have been extensively studied due to their nontoxicity and effectiveness compared to conventional solvents. These solvents can also be used to remove different types of endodontic sealers. Bioceramic-based sealers have become increasingly popular; however, they are difficult to dissolve. Objective: The aim is to examine the differences in efficacy between eucalyptus oil (Eucalyptus globulus), sweet orange oil (Citrus sinensis), and grapefruit oil (Citrus paradisi) as bioceramic sealer solvents. Methods: Thirty-two bioceramic sealer samples were placed in cylindrical stainless steel molds 1.5 mm in height and 8 mm in internal diameter and were placed in an incubator to set. After 72 h, the sealer samples were weighed to obtain their initial weights. Then, they were divided into three groups of ten, which were immersed in 1 ml of eucalyptus, sweet orange, or grapefruit oil for 1 min, and one group of two, used as negative controls. The sealer samples were placed in an incubator for 24 h to dry and weighed once more to obtain their final weights. The differences in weight were calculated and analyzed using one-way analysis of variance. Results: The analysis of the initial and final weight differences of the sealer samples immersed in the three essential oils revealed no statistically significant differences between them. Conclusions: The three essential oils have comparable efficacies as bioceramic sealer solvents.

Evaluating the effect of aromatherapy on a stress marker in healthy subjects

Background/purposeChemotherapy is important for cancer treatment, but patients’ physical and mental stress may lead to unfavorable pain control, an increase in the risk of relapse, and a reduction in the quality of life (QOL). Recently, aromatherapy has been performed in addition to palliative care in many countries, such as Japan and the United States, but scientific evidence remains insufficient. To investigate the usefulness of aromatherapy as complementary and alternative medicine, we evaluated its influence on the immune and autonomic nervous systems.MethodsWe instructed healthy volunteers to inhale aroma oil at bedtime for 6 weeks, and measured changes in the salivary level of secretory immunoglobulin A (s-IgA). Furthermore, blood was collected in addition to saliva in some healthy volunteers, and the blood level of noradrenaline (NA) was measured to examine its relationship to changes in the salivary s-IgA level.ResultsAromatherapy with lavender and grapefruit oils significantly increased the salivary s-IgA level: lavender oil increased 3.5-fold (p = 0.03), and grapefruit oil increased 2.55-fold (p = 0.04). On lavender oil inhalation, there was a weak, positive correlation between changes in the salivary s-IgA level and those in the blood NA level (R2 = 0.24).ConclusionThe results showed that aromatherapy with lavender and grapefruit oils reduced stress by acting on the immune and autonomic nervous systems in healthy volunteers. In the future, its clinical usefulness must be investigated through similar examination in patients in whom the stress level may be high.

PREPARATION AND CHARACTERIZATION OF GRAPEFRUIT OIL BASE MICROEMULSIONS OF CAFFEINE

Objective: The objective of the present work was to prepare and characterize grapefruit oil base microemulsions loaded with caffeine as a model hydrophilic compound.
 Methods: The formulation ingredients were selected based on surfactant efficiency and solubility studies. Ternary phase diagrams of grapefruit oil were constructed using the water titration method. Nine O/W microemulsions were constructed and prepared by mixing surfactant system, grapefruit oil, water and caffeine together. The resulting microemulsions were investigated for viscosity using Brookfield viscometer, for pH value using a digital pH meter, and for average particle size and polydispersity index (PDI) using a Zetasizer Nano. Ex vivo skin permeation through porcine ear skin was conducted using a side-by-side diffusion cell. The amount of caffeine was analyzed using HPLC-UV method.
 Results: Tween 20 yielded the highest emulsification ability for grapefruit oil and the highest caffeine solubility. It was selected as a major surfactant. Caffeine was slightly soluble in ethanol and isopropyl alcohol, but sparingly soluble in propylene glycol (PG). These ingredients were used as the cosurfactants. Nine grapefruit oil base microemulsions were prepared and characterized. The pH of microemulsions was within the range of 4.48-5.96. Particle size was in the range of 10.81±0.03 to 62.18±21.04 µm with the PDI of 0.13±0.02 to 0.64±0.11. Viscosity and particle size of microemulsions increased significantly with increasing grapefruit oil or tween 20 content. Addition of PG as cosurfactant resulted in the increases of viscosity, particle size and PDI. Depending on the formulation parameters, the permeation fluxes of caffeine from grapefruit oil base microemulsions were in the range of 28.4±3.4-361.4±15.2 µg/cm2/h.
 Conclusion: The grapefruit oil base microemulsions were successfully formulated. The physical properties and caffeine permeation of these microemulsions were found to be dependent on the grapefruit oil content, tween 20 content, cosurfactant type and content, as well as caffeine loading. The optimal formulation of grapefruit oil base microemulsion suggested composition of 5% grapefruit oil, 50% surfactant system (tween 20 and ethanol at the ratio of 9:1), and water.

Biological Activities and Safety of Citrus spp. Essential Oils.

Citrus fruits have been a commercially important crop for thousands of years. In addition, Citrus essential oils are valuable in the perfume, food, and beverage industries, and have also enjoyed use as aromatherapy and medicinal agents. This review summarizes the important biological activities and safety considerations of the essential oils of sweet orange (Citrus sinensis), bitter orange (Citrus aurantium), neroli (Citrus aurantium), orange petitgrain (Citrus aurantium), mandarin (Citrus reticulata), lemon (Citrus limon), lime (Citrus aurantifolia), grapefruit (Citrus × paradisi), bergamot (Citrus bergamia), Yuzu (Citrus junos), and kumquat (Citrus japonica).

Effect of olfactory stimulation with essential oils on cardiovascular reactivity during the moving beans task in stroke patients with anxiety

ObjectiveTo investigate the effect of olfactory stimulation with essential oils on cardiovascular reactivity during the “moving beans” rehabilitation task in stroke patients with anxiety. MethodsTwenty-eight stroke patients participated in this study. Blood pressure and heart rate were measured before and after finger movement tasks (e.g., moving beans and the Purdue pegboard test). Olfactory stimulation with lavender oil, grapefruit oil, and distilled water were conducted during finger tasks. Anxiety was assessed using the State Trait Anxiety Inventory (STAI)-Y2 before the finger movement tasks. ResultsThere were no significant changes in blood pressure or heart rate activity in both finger movement tasks when stimulation of lavender oil, grapefruit oil, and distilled water was applied. However, the change values of Δ diastolic blood pressure (DBP) associated with the moving beans task indicated a significant interaction between olfactory stimulations and the groups of STAI-Y2 scores (high vs low) (p=0.03), without main effects in the olfactory stimulations and the groups of STAI-Y2 scores. ConclusionOlfactory stimulation with lavender and grapefruit oil may repress the exaggerated DBP response during the moving beans task in stroke patients with higher levels of trait anxiety symptoms.

Corrosion inhibition of carbon steel in acidic medium by Grapefruit oil extract

Carbon steel is widely used in various fields such as the field of agri-food their use in tins and materials used in the food producing seen this material and susceptible to corrosion phenomena are in their environmental which produces toxic compounds in the environmental medium of this material. To this end we investigated in this direction by study of the inhibition of the phenomenon of corrosion of this material. Grapefruit oil extract (GO) was tested as corrosion inhibitor for mild steel in 1M HCl. Electrochemical impedance spectroscopy (EIS) method are used. The inhibiting action increases with the concentration of the tested inhibitor. The highest efficiency 86.15% is obtained at 1g/L of GO. Effect of temperature is also studied between 298 K and 328 K; GO presents best inhibition efficiency even at elevated temperature, GO is adsorbed on the steel surface according to a Langmuir isotherm adsorption model. The thermodynamic data of activation are determined. In this study fatty acid composition contents of Grapefruit were determined. The used methods indicate that the Grapefruit oil extract is a good inhibitor in 1.0 M HCl. Some thermodynamic functions of the carbon steel dissolution process in acid medium were also determined and discussed.