Grand Park Research Articles

A Study on Estimating Theme Park Attendance Using the AdaBoost Algorithm Based on Weather Information from the Korea Meteorological Administration Web

The purpose of this study is to propose an efficient machine learning model based on five years of data for Seoul Grand Park in Republic of Korea, depending on the weather and day characteristics, and to increase its effectiveness as a strategic foundation for national theme park management and marketing. To this end, the AdaBoost model, which reflects the characteristics of the weather and the day of the week, was recently compared with the actual number of visitors and the predicted number of visitors to analyze the accuracy. The analysis showed 30 days of abnormal cases, and the overall annual distribution was found to show similar patterns. Abnormal cases required details of wind speed, average relative humidity, and fine dust concentration for weather information, and it was derived that more accurate predictions would be possible considering variables such as group visitors, new events, and unofficial holidays.

Pedobacter faecalis sp. nov., isolated from the faeces of eland, Taurotragus oryx.

Strain ELA7T, a novel Gram-negative, non-motile bacterium with a white pigment and rod-shaped morphology, was isolated from the faeces of an eland at Seoul Grand Park, a zoo in the Republic of Korea. The novel bacterial strain grew optimally in R2A medium under the following conditions: 0 % (w/v) NaCl, pH 8.0, and 34 °C. Based on phylogenetic analyses using 16S rRNA gene sequencing, strain ELA7T was found to have the closest relatedness to Pedobacter ginsengisoli Gsoil 104T (97.8 %), P. frigoris RP-3-15T (97.2 %), P. humi THG S15-2T (97.0 %), P. seoulensis THG-G12T (97.0 %), and P. foliorum LMG 31463T (96.9 %). The genome size and genomic DNA G+C content of strain ELA7T were 3.63 Mbp and 46.5 %, respectively. A whole genome-level comparison of strain ELA7T with P. ginsengisoli Gsoil 104T, P. frigoris RP-3-15T, P. africanus DSM 12126T, and P. psychroterrae RP-1-14T revealed average nucleotide identity values of 72.0, 71.8, 71.9, and 71.6 %, respectively. The major fatty acids were summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c) and MK-7 was the predominant respiratory quinone. The major polar lipids of strain ELA7T were phosphatidylethanolamine, sphingolipid, unidentified aminolipid, unidentified phosphoglycolipid, unidentified glycolipid, and eight unidentified lipids. Considering our chemotaxonomic, genotypic, and phenotypic findings, strain ELA7T (=KACC 23137T=JCM 36003T) is identified as representing a novel species within the genus Pedobacter, for which the name Pedobacter faecalis sp. nov. is proposed.

The Grand Park Hotel: Using Accountability and Inclusive Leadership to Save a Failing Business

When a business is not performing well, the biggest challenge of the leader is to determine the root cause of the issues and address each issue to turn performance around. This case study focuses on the deterioration of a hotel once considered one of the “grandest hotels in New England,” and the efforts undertaken by its new general manager, Barbara Jones, to return the hotel to its earlier stature of grandeur, superior customer service, and financial performance. The case study requires students to understand the role trust, accountability and inclusive leadership play to heighten employee engagement, manage conflict, and improve overall organizational performance.

서울기록원 소장 남산공원 기록물의 현황과 내용†

Namsan Park in Seoul was designated as a “grand park” in 1954 and is currently operated as an ‘Urban Nature Park Area’ and four ‘neighborhood parks.’ However, despite the park’s historical and cultural value as an urban park, it has been discussed mainly from a perspective revolving around notions of a mountain or a city wall. To ensure a comprehensive exploration of Namsan Park’s history, this study examined public records at the Seoul Metropolitan Archives (SMA), which houses the city’s permanent records for preservation and organization. To this end, documents in the SMA Database (DB) were analyzed, yielding 1,359 records concerning Namsan Park. Based on the contents, general characteristics of the urban park were identified through production periods, record types, and disclosure types. Then, essential keywords concerning organizations, people, geographical areas, subjects, and business functions were examined. Finally, the contents and characteristics of Namsan Park in public records were scrutinized, focusing on specific spaces. This research also uncovered important information, such as park drawings, photos, planting lists, plant parcel lists, and significant discussions and decisions regarding the operation and management of the park. Although the public records do not contain a comprehensive history of Namsan Park, it was possible to check the primary historical changes and deliberation processes pertaining to the park’s history. Therefore, continuous research intended to interpret and describe public records is expected to identify many implications. In addition, because the public records showed heterogeneous characteristics that center on specific periods and events, an essential task is to advance collaboration and networking with various related institutions, designers, researchers, and citizens.

The Effects of Particulate Matter Alert on Urban Park Visitation in Seoul, Korea: Using Segmented Regression.

To reduce the health burden from particulate matter (PM), the Korean government implemented a nationwide PM10 (particles less than 10 µg/m3 in diameter) alert system in 2015. The policy was intended to reduce PM exposure by advising people to refrain from outdoor activities on highly polluted days. The present study aimed to estimate the effect of the PM10 alert system on people's daily outdoor activity patterns using urban park (specifically, Children's Grand Park) visitation data from Seoul, South Korea, from 2014-2019. Segmented regression was fitted to estimate whether the number of visitors to the park decreased on the days with PM10 alerts. PM10 concentration of 80 µg/m3, the cut-off point for a "Bad" alert, was set as a threshold, and discontinuity at the threshold and change in the relative risk after the threshold was tested. Time series regression was used to estimate the dose-response line between the ambient PM10 concentration and the daily number of park visitors. The number of park visitors decreased by 11.8% (relative risk: 0.881, 95% confidence interval: 0.808, 0.960) when a "Bad" alert was issued (PM10 level above 80 µg/m3) compared to when the alert level was "Normal" (PM10 level less than 80 µg/m3). The present study found evidence that the PM10 alert influenced people's daily outdoor activities in Seoul, Korea. As the main purpose of the PM alert is to encourage people to refrain from outdoor activities, evaluating the relationship between PM alerts and behavior patterns can help to grasp the effectiveness of the policy. Further efforts should be made to investigate whether the observed behavioral change leads to reductions in health outcomes caused by PM.

Evaluation of the effects of particulate matter forecasting system on behavior patterns in Seoul, Korea

Background and aim: The particulate matter (PM) forecasting system is one of the most prominent political options that can help to reduce the risk of PM. Its goal is to urge people to take avoidance behaviors for upcoming pollution by issuing warnings. In Korea, the national PM10 forecasting system was launched on February 6, 2014. We aimed to estimate the effectiveness of the PM forecasting system in relation to the number of visitors to the Children’s Grand Park in Seoul, Korea. Methods: We used regression discontinuity design to estimate the effectiveness of the policy from 2014 to 2019. Since the PM forecasting results were not available, the measured PM10 concentration of Seoul was used as a proxy for treatment variable. The cut-off point of the intervention was set to 80ug/m3, the starting point for a "Bad” rating, and the bandwidth was set to 10ug/m3. We further adjusted the daily mean temperature. Results: When the PM10 concentration exceeds 80ug/m3, the number of visitors to the park decreased by about 15,000. In addition, there was a negative relationship between PM10 concentration and the number of park visitors where pollution levels were over 80ug/m3. The results were statistically significant at the 10% significance level. Conclusions: Our result indicates that the PM10 forecasting system seems effective in Korea. According to previous studies in Korea, the risk of PM10 on various health outcomes decreased in recent years. The introduction of the forecasting system and increment in awareness of PM might be possible causes for that change in the PM10 risk.

Mapping Susceptibility of Forest in Taman Raya Raden Soerjo Using Machine Learning Random Forest

The increasing incidence of forest fires in Raden Soerjo’s Grand Park over the last 5 years has become a concern for conducting research on fire predictions. The availability of free remote sensing data makes it easier to analyze forest fires using Geographic Information Systems (GIS). and remote sensing. Varying publicly available spatial datasets were used to classify forest fires affected areas with an approach of Machine Learning (ML) using Random Forest (RF). Nine (9) variables suspected as potential cause of forest fires (NDVI. ET. LST. Aspect. Slope. Altitude. Distance to Road (ED_Road). Distance to Built-up area (ED_BUA). and Distance to river (ED_River) were used as determinants. About 80% of the data were used as Trained data and 20% as a validation. The results of the model produced an accuracy of 0.96. The model result was checked for its sensitivity with the Area Under the Curve (AUC) AUC results with a value of 0.89. Findings show that Random Forest could be applied to map forest fire severity classes with a good result.

Individuals Matter: Dilemmas and Solutions in Conservation and Animal Welfare Practices in Zoos.

Simple SummaryReaching conservation objectives while upholding individual animal welfare standards presents a significant challenge for zoos, especially if some individual animal interests conflict with their conservation mission. However, the compassionate conservation movement offers a potential solution for facing such challenges by advocating for the consideration of individual animal interests as central to conservation decision making. Our objective is therefore to determine to what extent zoological parks recognize the intrinsic value of zoo animals, beyond being members of species or ecosystems, and how this recognition manifests. Through discourse analysis, interviews, and relevant printed sources, we analyze the discourses, or concepts and categorizations, by which actors—experts in the conservation, animal rights, welfare, and zoo fields—give meaning to zoo practices. We demonstrate ways in which these discourses shape the captivity, breeding, and culling practices of individual zoo animals in the name of conservation. We found that people justifying these practices within zoos fail to recognize the intrinsic value of individual animals beyond being members of species. However, within the zoo, welfare practices and education objectives increasingly focus on fulfilling individual animal interests.Compassionate conservation advocates for minimizing individual suffering in conservation practice and adheres to the principle “individuals matter”—intrinsically, in and of themselves. Our objective is to determine the extent to which, and how, zoos recognize the intrinsic value of wild individuals beyond their status as members of species or ecosystems. We analyzed discourses surrounding the Smithsonian National Zoo in the U.S.A., the zoos of the Muséum National d’Histoire Naturelle in France, and the Seoul Grand Park Zoo in South Korea. Using existing literature on zoos, conservation, animal welfare, and rights, we distilled two discourses (justificatory and abolitionist). Through interviews with professionals in the zoo, conservation, welfare, and animal rights communities, we demonstrate how actors frame individual zoo animals as (1) sentient persons, (2) reproductive components, and (3) species ambassadors. Our analysis shows how actors’ views shape three zoo practices related to ex situ conservation: (1) captivity, (2) captive breeding, and (3) culling. This analysis revealed two significant findings. First, actors representing the justificatory discourse fail to frame animals as intrinsically valuable individuals. Second, within the constraints of the zoo, the intrinsic value of individual animals is recognized through welfare practices and education focused on fulfilling animal interests.

Multi-Class Parrot Image Classification Including Subspecies with Similar Appearance.

Simple SummaryOwing to climate change and human overdevelopment, the number of endangered species has been increasing. To face this challenge, the CITES treaty has been adopted by many countries worldwide to prevent the extinction of endangered plants and animals. Additionally, since customs clearance inspections for goods at airports and ports take a long time, and due to the difficulty of distinguishing such species by nonexperts, smugglers have been exploiting this vulnerability to illegally import or export endangered parrot species. If these cases continue to increase, the extinction of species with fewer populations can be accelerated by illegal trade. To tackle this problem, in this study, we constructed an object detection model using convolutional neural networks (CNNs) to classify 11 endangered species of parrots. Utilizing artificial intelligence techniques, the procedures for inspection of goods can be simplified and the customs clearance inspection systems at airports and ports can be enhanced, thus protecting endangered species.Owing to climate change and human indiscriminate development, the population of endangered species has been decreasing. To protect endangered species, many countries worldwide have adopted the CITES treaty to prevent the extinction of endangered plants and animals. Moreover, research has been conducted using diverse approaches, particularly deep learning-based animal and plant image recognition methods. In this paper, we propose an automated image classification method for 11 endangered parrot species included in CITES. The 11 species include subspecies that are very similar in appearance. Data images were collected from the Internet and built in cooperation with Seoul Grand Park Zoo to build an indigenous database. The dataset for deep learning training consisted of 70% training set, 15% validation set, and 15% test set. In addition, a data augmentation technique was applied to reduce the data collection limit and prevent overfitting. The performance of various backbone CNN architectures (i.e., VGGNet, ResNet, and DenseNet) were compared using the SSD model. The experiment derived the test set image performance for the training model, and the results show that the DenseNet18 had the best performance with an mAP of approximately 96.6% and an inference time of 0.38 s.

Microsatellite characterization and development of unified STR panel for big cats in captivity: a case study from a Seoul Grand Park Zoo, Republic of Korea.

The zoos manage small populations of endangered big cat species like tiger, lion, and leopard for display, research, and conservation breeding. Genetic management of these populations is essential to ensure long term survival and conservation utility. Here we propose a simple and cost effective microsatellite based protocol for the genetic management of captive big cats. We sampled 36 big cat individuals from Seoul Grand Park Zoo (Republic of Korea) and amplified 33 published microsatellite loci. Overall, allelic richness and gene diversity was found highest for leopards, followed by lions and tigers. Twelve of the thirty-three markers showed a high degree of polymorphism across all target species. These microsatellites provide a high degree of discrimination for tiger (1.45 × 10-8), lion (1.54 × 10-10), and leopard (1.88 × 10-12) and thus can be adopted for the genetic characterization of big cats in accredited zoos globally. During captive breeding, zoo authorities rely on pedigree records maintained in studbooks to ensure mating of genetically fit unrelated individuals. Several studies have reported errors in studbook records of big cat species. Microsatellites are simple and cost effective tool for DNA fingerprinting, estimation of genetic diversity, and paternity assessment. Our unified microsatellite panel (12-plex) for big cats is efficient and can easily be adopted by zoo authorities for regular population management.

Flaviflexus ciconiae sp. nov., isolated from the faeces of the oriental stork, Ciconia boyciana.

A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea. Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively. Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids. The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids. The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine. The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %. Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp. nov. is proposed. The type strain is H23T48T (=KCTC 49253T=JCM 33282T).

COVID-19: Return to Youth Sports

COVID-19 has impacted all areas of life, and youth sports is no exception. States and counties are publishing their own unique guidelines for permitting youth sports to return over designated phases, creating a patchwork of guidelines and dates for returning to practice and games. Governing bodies, sports facilities, and event operators are creating modifications and adaptations for participants and spectators to ensure a safe environment. The Sports Innovation Institute at IUPUI, a partnership between Indiana and Purdue universities in Indianapolis, and Grand Park Sports Campus (Westfield, Ind.) collaborated to better understand how COVID-related adaptations are perceived by parents, athletes, coaches, officials, and administrators. The results provide youth sports facilities and event operators with data on how specific adaptions are received by these stakeholders who are looking to return to youth sports in a timely, but safe manner.
 Twelve adaptations were identified from a review of documents prepared by states, governing bodies, trade associations, media reports, and feedback from industry and academic experts. The survey questions were designed using the Kano Model (pronounced “kah-no”), which was selected due to its ability to determine how people feel about proposed adaptions during the COVID-19 pandemic. Each adaption comprised three questions that assessed the respondents’ feelings toward the adaptation (functional question), their feelings if the adaptation did not exist (dysfunctional), and their assessment of how important it is for the adaptation to occur (importance). The Kano Model is interpreted based upon these three scores, and each adaptation can be placed into one of five categories on a scatterplot.
 The survey was distributed to 40 organizations that circulated the survey to their members. The survey reached a national audience that represents the landscape of youth sports. A total of 10,359 people from 45 states completed the entire survey, representing at least 13 different sports. Nearly 92% of respondents were parents, but with the option to select multiple roles, coaches (25%), administrators (10%), athletes (9%), and officials (3%) were also represented. 
 Results indicated that venues and events should invest heavily and visibly in sanitization of the facility, playing areas, and equipment before, during, and after events. Venue operators and event managers can feel confident the recommendations provided by the Centers for Disease Control and Prevention (CDC) and National Federation of State High School Associations (NFHS) to sanitize playing areas and equipment after each use will be well-received and welcomed by users. Promotion and monitoring of social-distancing guidelines, limiting personal contact between players, limiting admission to those under age 65 with no CDC-indicated pre-existing conditions, and completing a health and contact-information questionnaire prior to entering are seen by users as must-be adaptations in order for players and spectators to feel comfortable returning to youth sports during the COVID-19 pandemic. This means their presence does not bring satisfaction, but their lack of presence brings significant dissatisfaction. Respondents felt indifferent toward changing arrival and departure routines, closing amenities, and minimizing the capacity and rearranging bench areas for athletes. The presence or absence of these adaptations do not make a real difference in users’ experiences. User sentiment regarding facemasks was mixed, with strong feelings about the use and non-use of facemasks. Finally, limiting entry to athletes and game personnel but excluding spectators was not well-received by survey respondents, especially parents. Youth sports venues and events should tread lightly when considering not allowing spectators into venues, and expect negative backlash from parents should such policies be adopted.
 Parents of recreational athletes viewed the adaptations in a more positive light and as a more necessary part of the youth sports experience than parents of travel athletes. A similar trend was found when comparing parents who are less willing to travel during the pandemic than those who do not expect their travel to be impacted. Travel sports parents demonstrated an increasing comfort level in traveling for competitions over the summer months, from 42% in May to 76% in August. The economic turmoil wrought by COVID-19 has touched nearly every component of American life. However, 59% of travel sports parents reported that the pandemic will not negatively impact their sports travel budget. Only 23% will experience a budget decrease greater than 25% related to youth sports travel.

The Real-Time Mobile Application for Classifying of Endangered Parrot Species Using the CNN Models Based on Transfer Learning

Among the many deep learning methods, the convolutional neural network (CNN) model has an excellent performance in image recognition. Research on identifying and classifying image datasets using CNN is ongoing. Animal species recognition and classification with CNN is expected to be helpful for various applications. However, sophisticated feature recognition is essential to classify quasi-species with similar features, such as the quasi-species of parrots that have a high color similarity. The purpose of this study is to develop a vision-based mobile application to classify endangered parrot species using an advanced CNN model based on transfer learning (some parrots have quite similar colors and shapes). We acquired the images in two ways: collecting them directly from the Seoul Grand Park Zoo and crawling them using the Google search. Subsequently, we have built advanced CNN models with transfer learning and trained them using the data. Next, we converted one of the fully trained models into a file for execution on mobile devices and created the Android package files. The accuracy was measured for each of the eight CNN models. The overall accuracy for the camera of the mobile device was 94.125%. For certain species, the accuracy of recognition was 100%, with the required time of only 455 ms. Our approach helps to recognize the species in real time using the camera of the mobile device. Applications will be helpful for the prevention of smuggling of endangered species in the customs clearance area.

3D SURVEYING AND 3D RECONSTRUCTION OF ARCHITECTURE OF THE ROYAL PARK OF TIRANA

Abstract. The research proposes the study of the Royal Park of Tirana in Albania, with particular reference both to the vegetation system, such as tree-lined avenues, ornamental gardens and vegetable gardens, and to the architectural elements such as the Villa, the Church, the gardener's house, the generator house and greenhouses. The few iconographic documents consist of the plans of the Grand Park of Tirana in 1957 and 1980, where some large buildings can be identified. The bibliography is relegated to the few citations found in the volumes illustrating the works of the two architects G. Bosio and G. Bertè. For the activities of knowledge of the Royal Park and of the architectural elements, in addition to the classical techniques of direct and indirect survey, we took into account the Ryobi laser instrumentation applied on portable computer support both tablets and smartphone. This digital system allows an immediate view of the relevant data on the photographic image taken by the support, transforming it into a dynamic datum. In the first phase, the relevant activity envisaged a basic knowledge extended to architectural organisms and green systems with a primitive geometric model; subsequently, measurements of architectural details, structures and the digital model were made. Appropriate photographic documentation was produced in addition to bibliographic, archival, and iconographic surveys.

EDITORIAL

It is with great honor that we edit the proceedings of “8th World Conference on Business, Economics and Management (BEM-2019)”, Grand Park Lara Hotel Convention Center, Antalya, Turkey, 26-28 April 2019. This privileged scientific event has contributed to the field of ELT for the eight year.
 As the guest editors of this issue, we are glad to have received a variety of articles focusing on Accounting, International Finance, Advertising Management, Labor Economics, Business & Economics, Labor Relations & Human Resource Management, Business Ethics, Law and Economics, Business Intelligence, Management Information Systems, Business Information Systems, Management Science, Business Law, Market Structure and Pricing, Business Performance Management, Marketing Research and Strategy, Business Statistics, Marketing Theory and Applications, Change Managementi Operations Research, Communications Management, Organizational Behavior & Theory, Comparative Economic Systems, Organizational Communication, Consumer Behavior, Prices, Business Fluctuations, and Cycles, Corporate Finance and Governance, Product Management, Corporate Governance, Production and Organizations, Cost Management, Production/Operations Management, Decision Sciences, Public Administration and Small Business Entrepreneurship, Development Planning and Policy, Public Choice, Economic Development, Public Economics and Finance, Economic Methodology, Public Relations, Economic Policy, Public Responsibility and Ethics, E-Bussiness, Regulatory Economics, E- Marketing, Resource Management, Economic Systems, Strategic Management, Entrepreneurship, Strategic Management Policy, Finance & Investment, Stress Management, Financial Economics, Supply Change Management, Global Business, Systems Management, Global Marketing, Systems Thinking, Growth; Aggregate Productivity, Taxes (related areas of taxes), Household Behavior and Family Economics, Technological Change; Research and Development, Human Resource, Technology & Innovation, Industrial and Manufacturing Engineering, Time Management, Information Systems, Total Quality Management, Information Technology Management, Travel/Transportation/Tourism, International Business, Welfare Economics, International Economics etc. Furthermore, the conference is getting more international each year, which is an indicator that it is getting worldwide known and recognized. Scholars from all over the world contributed to the conference. Special thanks are to all the reviewers, the members of the international editorial board, the publisher, and those involved in technical processes. We would like to thank all who contributed to in every process to make this issue actualized. A total of 45 full papers or abstracts were submitted for this conference and each paper has been peer reviewed by the reviewers specialized in the related field. At the end of the review process, a total of 12 high quality research papers were selected and accepted for publication.
 I hope that you will enjoy reading the papers.
 Best Regards
 
 Guest Editors
 Prof. Dr. Cetin Bektas, Gaziosmanpasa University, Turkey
 Editorial Assistant
 Zeynep Genc, Phd. Istanbul Aydin University, Istanbul, Turkey

Iodobacter ciconiae sp. nov., a bacterium isolated from feces of oriental stork, Ciconia boyciana.

A novel Gram-stain-negative, facultatively anaerobic, non-motile, non-violet-pigmented, rod-shaped bacterium, designated strain H11R3T, was isolated from the feces of Oriental stork, Ciconia boyciana, collected from Seoul Grand Park Zoo, Republic of Korea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that H11R3T formed a monophyletic clade with Iodobacter fluviatilisDSM 3764T, Iodobacter arcticusDSM 100243T, and Iodobacter limnosediminisDSM 103822T, with sequence similarities of 98.8, 98.6 and 98.4 %, respectively. H11R3T grew optimally at 15 °C, pH 8, with 0.5 % (w/v) NaCl. The predominant isoprenoid quinone was ubiquinone-8 (Q-8), and polar lipids included phosphatidylethanolamine, three unidentified lipids, four unidentified phospholipids, and two unidentified aminophospholipids. The major fatty acids were summed feature 3 and C16 : 0, and the DNA G+C content of the genome is 48.0 mol%. The average nucleotide identity (ANI) value between strains H11R3T and I. fluviatilis NCTC 11159T (=DSM 3764T) is 83.7 %. On the basis of phenotypic, genotypic, phylogenetic and chemotaxonomic characteristics, strain H11R3T represents a novel species of the genus Iodobacter for which the name Iodobacterciconiae sp. nov. is proposed. The type strain is H11R3T (=KCTC 62666T=JCM 33283T).

Phenotypic and Genomic Properties of Brachybacterium vulturis sp. nov. and Brachybacterium avium sp. nov.

Two strains, VM2412T and VR2415T, were isolated from the feces of an Andean condor (Vultur gryphus) living in Seoul Grand Park, Gyeonggi-do, South Korea. Cells of both strains were observed to be Gram-stain positive, non-motile, aerobic, catalase positive and oxidase negative. Growth was found to occur at 10-30°C, showing optimum growth at 30°C. The strains could tolerate up to 15% (w/v) NaCl concentration and grow at pH 6-9. The strains shared 99.3% 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 89.0-89.2% ANIb, 90.3% ANIm, 89.7% OrthoANI and 38.0% dDDH values calculated using whole genome sequences. Among species with validly published names, Brachybacterium ginsengisoli DCY80T shared high 16S rRNA gene sequence similarities with strains VM2412T (98.7%) and VR2415T (98.4%) and close genetic relatedness with strains VM2412T (83.3–83.5% ANIb, 87.0% ANIm, 84.3% OrthoANI and 27.8% dDDH) and VR2415T (82.8–83.2% ANIb, 86.7% ANIm, 83.9% OrthoANI and 27.2% dDDH). The major fatty acid of the two strains was identified as anteiso-C15:0 and the polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, presumptively phosphatidylethanolamine and three unidentified glycolipids. Strain VR2415T also produced an unidentified phospholipid. The cell walls of the two strains contained meso-diaminopimelic acid as diagnostic diamino acid and the whole cell sugars were ribose, glucose, and galactose. The strains contained MK-7 as their predominant menaquinone. The genomes of strains VM2412T, VR2415T, and B. ginsengisoli DCY80T were sequenced in this study. The genomic G+C contents of strains VM2412T and VR2415T were determined to be 70.8 and 70.4 mol%, respectively. A genome-based phylogenetic tree constructed using an up-to-date bacterial core gene set (UBCG) showed that the strains formed a clade with members of the genus Brachybacterium, supporting their taxonomic classification into the genus Brachybacterium. Based on phenotypic and genotypic analyses in this study, strains VM2412T and VR2415T are considered to represent two novel species of the genus Brachybacterium and the names Brachybacterium vulturis sp. nov. and Brachybacterium avium sp. nov. are proposed for strains VM2412T (=KCTC 39996T = JCM 32142T) and VR2415T (=KCTC 39997T = JCM 32143T), respectively.

Oceanisphaera avium sp. nov., isolated from the gut of the cinereous vulture, Aegypius monachus.

A Gram-stain-negative, aerobic, catalase- and oxidase-positive, rod-shaped, flagellated bacterial strain, designated AMac2203T, was isolated from the gut of the cinereous vulture, Aegypiusmonachus, collected from the Seoul Grand Park Zoo, Republic of Korea. Strain AMac2203T grew optimally at 15-25 °C, pH 7-8 and in the presence of 3-5 % (w/v) NaCl. Phylogenetic analysis revealed 97.4-97.9 % and 96.9-97.3 % sequence similarities of the 16S rRNA genes to its counterparts in Oceanisphaera profunda SM1222T and Oceanisphaera ostreae T-w6T, respectively. The predominant fatty acids (>10 %) of strain AMac2203T were summed feature 3 (C16 : 0ω7c and/or C16 : 1ω6c, 33.6 %), summed feature 8 (C18 : 1ω7c, 24.5 %) and C16 : 0 (19.9 %). The primary isoprenoid quinone was ubiquinone-8. Polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified amino lipid and an unidentified lipid. Based on complete genome sequencing of strain AMac2203T and the closest related type strain, O. profunda, the OrthoANI value is 77.5 %, which is below the 95 % cut-off for species demarcation. The genomic DNA G+C content of strain AMac2203T is 47.1 mol%. Thus, strain AMac2203T represents a novel species candidate of the genus Oceanisphaera. We propose the name Oceanisphaeraavium sp. nov., with strain AMac2203T (=KCTC 62118T=JCM 32207T) as the type strain.

An outbreak of toxoplasmosis in squirrel monkeys (Saimiri sciureus) in South Korea.

Toxoplasma gondii (T.gondii) is an intracellular protozoan parasite that can infect warm-blooded animals including humans. New World monkeys, such as squirrel monkeys, are more susceptible to T.gondii than Old World monkeys, often developing fatal disease. In this study, seven of thirteen dead squirrel monkeys at Seoul Grand Park were tested to find the cause of sudden death. The main histopathological findings included interstitial pneumonia, necrotizing hepatitis, and splenitis. Periodic acid-Schiff staining of liver, spleen, and lung revealed cyst structures consistent with bradyzoites. Amplification of the B1 gene was detected in the liver or spleen of all monkeys. Additionally, a restriction fragment length polymorphism assay and phylogenetic analysis of the GRA6 amplicon revealed a consistent clustering with the type II strain of T.gondii. This study is the first report of T.gondii infection of squirrel monkeys in Korea, and the first report of type II T.gondii based on GRA6 analysis in Korea.

Tumebacillus avium sp. nov., isolated from the gut of a cinereous vulture, Aegypius monachus.

A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T, was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea). Strain AR23208T grew optimally at 25-30 °C, at pH 7 and in the absence of NaCl. Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T, respectively. The predominant fatty acids (>10 %) of strain AR23208T were iso-C15 : 0, summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I) and anteiso-C15 : 0 and the primary isoprenoid quinone was menaquinone-7. The polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, six unidentified phospholipids, an unidentified aminophospholipid and ten unidentified lipids. The sugar components of the cell wall peptidoglycan were ribose and arabinose. The amino acids of the cell wall peptidoglycan were l-alanine, aspartic acid, meso-diaminopimelic acid, l-glutamic acid, glycine and l-lysine. The OrthoANI value based on the complete genome sequence of strain AR23208T and the closest related strain, T. algifaecis THMBR28T, was 80.4 %. The genomic DNA G+C content of strain AR23208T was 56.0 mol%. Based on the data presented in the current study, strain AR23208T is considered to represent a novel species of the genus Tumebacillus, for which the name Tumebacillus avium sp. nov. is proposed. The type strain is AR23208T (=KCTC 33929T=JCM 32188T).